Labrador Retriever Dogs Research Articles

The Influence of Oxytocin on Maternal Care in Lactating Dogs.

Simple SummaryThe role of oxytocin in regulating uterine contractions and milk ejection has been largely outlined. However, its central functions in neuromodulating the onset and maintenance of maternal care in domestic dogs are still unclear. Since the mother–infant interactions have a severe impact in determining later behavior in mammals, this study intended to investigate the possible correlation between salivary oxytocin and maternal care in 25 lactating dogs. Only a negative correlation between salivary oxytocin and sniffing/poking behavior was found. Therefore, salivary oxytocin cannot be considered a strong predictive biomarker of the quantity of maternal care in dogs. Moreover, the percentage of male puppies showed a positive correlation with salivary oxytocin and lateral nursing, which suggests a sex-biased parental investment in this species. These findings can be considered the first piece of the oxytocin puzzle in regulating dog–pup interaction.In recent years, many studies on the role of oxytocin (OXT) in the onset of parental care, regulation of social bonding, and modulation of the emotional state have been published. However, its possible regulation of maternal behavior in lactating dogs has not been investigated yet. For this reason, the present study aimed at assessing potential correlations between salivary oxytocin and maternal behavior in 25 lactating Labrador Retriever dogs. Salivary concentrations of OXT (sOXT) were unrelated to the amount of maternal care except for a weak negative correlation with sniffing/poking behavior. Moreover, sOXT was positively correlated with the percentage of male puppies. Sniffing/poking behavior, in turn, was positively correlated with the duration of time the mothers spent out of the whelping box, while the number of male puppies showed a positive correlation with lateral nursing, a position known to provide puppies the easiest access to the milk. Taken together, these results suggest that sOXT may not be strongly associated with maternal care dynamics but could be correlated with sex-biased parental investment in dogs.

Platelet Indices and Erythrocyte Parameters in Healthy Police Dogs in Khartoum State – Sudan

Normal levels of platelets and erythrocytes parameters and the effect of breed and sex on them in healthy police dogs were determined. Forty-six dogs were used; 20 Labrador Retriever and 26 German Shepherd dogs comprising 21 males and 25 females, 20-83 months old. Five ml blood samples were collected from the cephalic vein; and analyzed using BK6100 Auto Hematology Analyzer. German Shepherd dogs showed significantly (p≤0.05) higher values than Labrador Retriever dogs for Mean platelet volume (MPV) (10.43±0.71vs. 9.9±0.73 FL), Red blood cells count (RBCs) (6.75±0.26 vs. 6.51±0.27 x1012 /L), and Hemoglobin concentration (HGB) (180.81±9.53vs.172.4±11.98 g/L), respectively. Highly positive significant correlations (p≤0.01) were found between Platelet count (PLT) and Plateletcrit (PCT) in males, females, and all dogs, and between MPV and Platelets distribution width (PDW) in all dogs. A highly positive significant correlation (p≤0.01) was found between MPV and PCT in all dogs. A highly negative significant correlation (p≤0.01) was found between PDW and RBCs and Red cell blood distribution width- index (RDW-CV) in males and all dogs. Also, a highly positive significant (p≤0.01) correlation was found between PDW and Mean corpuscular volume (MCV) in all dogs. A highly significant negative correlation (p≤0.01) was found between MPV and RBCs in males. A highly negative significant correlation (p≤0.01) was found between MPV and RDW (CV) in males. However, highly positive significant correlations (p≤0.01) were found between MPV and Hematocrit (HCT) in females. The breed may influence platelets and erythrocyte parameters and this should be considered in clinical interpretations.

Timoma en perro: Reporte de caso

Thymoma is a tumor that arises from cells of the thymus, an organ present in all mammals, responsible for the maturation of T lymphocytes and that regresses in size throughout life, until in adulthood it is gradually replaced by adipose tissue. These tumors are more common in elderly animals and the clinical signs associated with this type of neoplasia are often non-specific until they reach large proportions. A preliminary diagnosis of thymoma is often made based on the location of the tumor and aspiration cytology. Compared to other types of tumor that can occur in this region, thymomas usually have a good prognosis with treatment. This article reports the case of a 10-year-old Labrador Retriever dog, diagnosed with thymoma of epithelial origin, who was treated as a thymectomy through intercostal thoracotomy with rib resection. Thymoma was identified through imaging exams such as chest radiography and computed tomography, by aspiration cytology and a diagnosis was confirmed through histopathological examination. The patient had a favorable evolution in the postoperative period and until now, she is in excellent general condition.

Does the systemic administration of l-arginine affect dental implant stability in nicotine consumer dogs?

BackgroundNicotine can have detrimental effects on dental implant osseointegration. This study aimed to evaluate the influence of systemic l-arginine supplement on the osseointegration of dental implants in nicotine consumer dogs.MethodsTwelve 1-year Labrador Retriever dogs had their right and left third and fourth mandibular premolars removed, and the sockets were left to heal for 6 months. Dogs were randomly divided into three groups (n = 16): group 1—0.2 mg/kg nicotine was injected twice daily; group 2—0.2 mg/kg nicotine was injected twice daily in addition to 200 mg/kg l-arginine capsules taken orally; and group 3—placebo. Forty-eight dental implants were inserted into the healed sockets of the dog’s mandible and were assessed by implant stability quotient (ISQ) using resonance frequency analysis (RFA) during 4 weeks and insertion and removal torque value analysis.ResultsNo implant failure occurred during the study period. The change in torque value between insertion and removal was similar in the placebo and nicotine+arginine consumer dogs (p = 0.276), which shows a positive effect of arginine supplementation in nicotine consumers. There was a significant difference in torque value change between nicotine+arginine vs. nicotine consumers (p = 0.049) and placebo vs. nicotine (p = 0.003). After 4 weeks, the placebo had the most significant improvement in torque value (47.0 ± 16.9), followed by nicotine+arginine (25.1 ± 37.8), and the worst torque value was for the nicotine group (− 5.7 ± 24.0) pound per inch. The results show that except in the first week, there are significant differences in ISQ between the groups in different periods. ISQ in all of the groups has reduced at first but then increased over time. At the time of implant placement, insertion torque was significantly higher in the nicotine consumer group than the nicotine+arginine consumer group and placebo group (p = 0.020).ConclusionArginine supplementation promotes bone healing and implant primary stability by improving dental implant osseointegration biomechanical characteristics.

Correlation between sperm parameters and circulating thyroid hormones and testosterone concentrations in Labrador Retriever dog

Thyroid hormones physiologically regulate the semen quality, by altering testosterone concentrations, and different seminal parameters, as well as sperm motility, viability and semen volume. Serum thyroxine (T4), free thyroxine (fT4), thyroid-stimulating hormone (TSH) and testosterone (T) concentrations were measured in 26 adult Labrador Retriever dogs (n. 20 normospermic and n. 6 azoospermic groups), aged 3 ± 0.5 years old, to determine their specific references, by taking into account the related conventional and kinematic sperm parameters and clinical ultrasound evaluations. The mean values of T4, fT4, TSH and T concentrations, as soon as those of sperm parameters and clinical evaluations of normospermic group were in line with dog’s physiological range of literature data. Normospermic group showed significant correlations between T4 and T (r = −0.681; p < .01), sperm progressive motility (%) (r = −0.623; p < .01), and sperm non-progressive motility (%) (r = 0.625; p < .02). The azoospermic group showed higher T4, fT4, TSH and lower T concentrations, compared to normospermic group, and a positive correlation between T4 and T (r = 0.8548; p < .046). The use of breed-specific hormonal ranges and sperm parameters will improve the knowledge of their interaction in Labrador Retriever dogs, adding a new segment of scientific literature. Highlights Thyroxine (T4), free thyroxine (fT4), thyroid stimulating hormone (TSH) and testosterone (T) concentrations were measured in male dogs. Conventional and kinematic sperm parameters were also evaluated. Dogs were divided in 20 normospermic and n. 6 azoospermic Labrador Retrivers. Azoospermic group showed higher values of T4, fT4, TSH and lower values of T compared to normospermic Use of breed-specific hormonal ranges and sperm parameters will improve the knowledge of scientific literature.

Molecular characterization of exon region of type 2 diabetes associated gene (KCNJ11) in Labrador retriever dogs

Molecular characterization of exon region of type 2 diabetes associated gene (KCNJ11) in Labrador retriever dogs

Magnetic resonance imaging features of the biceps tendon rupture in a Labrador retriever dog

Magnetic resonance imaging features of the biceps tendon rupture in a Labrador retriever dog

Biomechanical comparison of standing posture and during trot between German shepherd and Labrador retriever dogs.

It is widely accepted that canine breeds stand and move differently. The prevalence of various musculoskeletal disorders such as hip and elbow dysplasia is also different between breeds. German shepherd dog (GSD) and Labrador retriever dog (LRD) are two large breeds with different conformations that have high prevalence of these disorders. This study quantifies the movement and standing posture of twelve healthy GSDs and twelve healthy LRDs to identify biomechanical similarities and differences that may be linked to sub-optimal hip and elbow mechanics. A pressure walkway and a motion capture system obtained measures of kinetics, kinematics and conformation during standing and trot. During standing, LRDs carry a greater percentage of the weight on the forelimbs (69%±5% vs. GSDs: 62%±2%, p<0.001) and their body Centre of Pressure (CoP) is located more cranially (p<0.001). GSDs had a greater pelvic tilt (79°±8 vs. 66°±9°, p = 0.004), more flexed stifles (44°±9° vs. LRDs: 34°±10°, p<0.05) and hocks (58°±11° vs. 26°±9°, p<0.01) and more extended hips (-10°±11° vs. 30°±12°, p<0.001). During trot, the GSDs’ CoP had a longer anterior-posterior trajectory (151%±22% vs. LRDs: 93%±25% of the withers height, p<0.001). Stride parameters and loading of limbs were similar when normalised to the size and weight of the dog, respectively. The LRDs had a more extended thoracolumbar angle (p<0.001) and a less flexed lumbosacral angle (p<0.05). The LRDs’ hip remained flexed during trot whereas the GSDs’ hip joint was less flexed during swing (p<0.001) and more extended in late stance and early swing (p<0.001). In conclusion, the LRDs and GSDs differ in the way they stand and move and this would result in different loading pattern of the joints. Further investigation is required to determine the extent to which biomechanical differences are linked to musculoskeletal problems presented clinically.

The Effect of a Moderate Exercise Program on Serum Markers of Bone Metabolism in Dogs.

Simple SummaryIn this article, we investigate the long-term (four months) effects of a 25 min moderate-intensity treadmill exercise, three times per week, on serum markers of bone metabolism (bone alkaline phosphatase and osteocalcin as bone formation markers and C-terminal telopeptide as bone resorption marker) in dogs. Twenty healthy dogs (Labrador and Golden Retrievers), median age 16.2 (11.7–24.4) months underwent the exercise program. Blood samples were collected as a baseline, mid-term (after two months) and at the end of the study. The values of bone formation markers significantly decreased following two months of exercise program; after which, bone alkaline phosphatase increased while osteocalcin concentration continued to decrease towards the end of the study. Bone resorption marker did not significantly change through the exercise duration. In overall, moderate exercise resulted in no change in bone resorption, and a mild bone formation in young developing dogs.The beneficial effect of physical activity on the musculoskeletal health in dogs is well recognized, but the level of intensity, duration, and frequency of exercise is not fully described. Measurement of serum markers of bone metabolism (bone alkaline phosphatase and osteocalcin as bone formation markers and C-terminal telopeptide as bone resorption marker) during four months of organized moderate-intensity physical training in Labrador retriever and Golden retriever dogs aged between 11.7–24.4 months, showed variations of bone metabolism. Dogs were included in treadmill running sessions for 25 min, three times per week. Blood samples were taken at the beginning of the program (baseline), after two months (mid-term) and at the end of the study after four months. The values of bone alkaline phosphatase and osteocalcin significantly decreased following two months of exercise program. Bone alkaline phosphatase increased by the end of four-month training cycle, but did not reach baseline value. Osteocalcin levels continued to decrease towards the end of the study. C-terminal telopeptide concentrations did not significantly change throughout the study duration. The results of this study show that aerobic exercise of moderate-intensity caused an initial decrease in bone formation followed by an increase of bone alkaline phosphatase and a further decrease of osteocalcin concentration. The response of two formation markers can be explained by the different stage of osteoblast activity that they express. In summary, moderate exercise resulted in no change in bone resorption, and a mild bone formation in young developing dogs.

Study of bilateral elbow joint osteoarthritis treatment using conditioned medium from allogeneic adipose tissue-derived MSCs in Labrador retrievers

Canine elbow dysplasia is a common cause of forelimb lameness in dogs and can lead to development of osteoarthritis (OA). A potential alternative to pain management is the use of a safe cell-free based therapy through trophic and paracrine factors of mesenchymal stem cells (MSCs). The aim of study was to identify the profile of selected mediators of potential clinical relevance in synovial fluid (SF) samples of dogs with elbow OA and analyse the range of motion (ROM) before and after cell-free MSCs-based treatment. In this study, conditioned medium from allogeneic canine adipose tissue - derived MSC (CM-AD-MSC) was prepared and administered into both elbow joints with OA in six Labrador retriever dogs (n = 6) on day 0 and 14 without creating a control group with a placebo. The SF of the elbow joints was analysed for the presence of several biomolecules (IL-6, IL-10, IL-8, IL-2, IL-12, TNF-αIFN-γ, MMP-3TIMP-1) before and after intraarticular applications of CM-AD-MSC. Kinematic analysis was used to assess the clinical effect of CM-AD-MSC. Analyses of SF and ROM were performed on days 0, 14 and 42. Concentration levels of MMP-3, TIMP-1, IL-6 and TNF-α in SF showed significant differences before and after the treatment (P < .05). There was a significant improvement in ROM between day 0 and 42 (P < .001). No severe adverse events were observed during the study. Results support the potential supportive effect of CM-AD-MSC as a noninvasive therapeutic tool for pain management of OA elbow joints in dogs.

Bayesian and Machine Learning Models for Genomic Prediction of Anterior Cruciate Ligament Rupture in the Canine Model.

Anterior cruciate ligament (ACL) rupture is a common, debilitating condition that leads to early-onset osteoarthritis and reduced quality of human life. ACL rupture is a complex disease with both genetic and environmental risk factors. Characterizing the genetic basis of ACL rupture would provide the ability to identify individuals that have high genetic risk and allow the opportunity for preventative management. Spontaneous ACL rupture is also common in dogs and shows a similar clinical presentation and progression. Thus, the dog has emerged as an excellent genomic model for human ACL rupture. Genome-wide association studies (GWAS) in the dog have identified a number of candidate genetic variants, but research in genomic prediction has been limited. In this analysis, we explore several Bayesian and machine learning models for genomic prediction of ACL rupture in the Labrador Retriever dog. Our work demonstrates the feasibility of predicting ACL rupture from SNPs in the Labrador Retriever model with and without consideration of non-genetic risk factors. Genomic prediction including non-genetic risk factors approached clinical relevance using multiple linear Bayesian and non-linear models. This analysis represents the first steps toward development of a predictive algorithm for ACL rupture in the Labrador Retriever model. Future work may extend this algorithm to other high-risk breeds of dog. The ability to accurately predict individual dogs at high risk for ACL rupture would identify candidates for clinical trials that would benefit both veterinary and human medicine.

Pharmacokinetics of acetaminophen after intravenous and oral administration in fasted and fed Labrador Retriever dogs.

Acetaminophen (paracetamol) is used in dogs to manage fever and mild pain. The aim of this study was to assess the pharmacokinetics of acetaminophen in both fed and fasted Labrador Retrievers after a single intravenous and oral administration (20mg/kg). Six healthy dogs underwent three treatments in a randomized block study (a, n=2; b, n=2; c, n=2). In phase one, group a received acetaminophen intravenously, group b and c orally after being fasted and fed, respectively. In phase two and three, groups were swapped, and the experiment was repeated. At the end of the trial, each dog received the same treatment. Acetaminophen plasma concentrations were detected using a validated HPLC-UV method. The pharmacokinetic analysis was performed using a noncompartmental model. Clearance, volume at steady state and half-life of acetaminophen in Labrador Retrievers were 0.42L/kghr, 0.87L/kg and 1.35hr, respectively. No significant statistical differences were found between fasted and fed dogs regarding maximum plasma concentration, time at maximum concentration and bioavailability as measured by the AUC. Feeding does not significantly affect the acetaminophen oral pharmacokinetics.

Dog-Human Play, but Not Resting Post-Learning Improve Re-Training Performance up to One Year after Initial Task Acquisition in Labrador Retriever Dogs: A Follow-On Study.

Simple SummarySituations that are emotional and arousing can have an effect on the memorability of events. The beneficial effect of dog–human play immediately after learning a new task has recently been shown to improve training performance in companion dogs (Labrador Retrievers) when required to solve the same task 24 h later. This follow-on study re-trained the same dogs after a period of one year in the same two-choice discrimination paradigm. Analyzed factors included: the age of the dogs, the effect of the trainer identity, training performance in the previous study, heart rate, and the number of trials and errors to meet the re-training criterion. The results show that all dogs relearned the task; however, dogs from the dog–human play group needed significantly fewer trials and made significantly less errors when compared to the control group. To the author’s knowledge, this is the first evidence that post-training activity may influence memory in dogs up to 1 year after the initial task acquisition. However, when interpreting the overall results, the limitations due to the low sample size must be taken into account.Arousing and emotional situations can improve cognitive performance and the memorability of events. Recently, the enhancement of training performance in Labrador Retriever dogs through 30 min of dog–human play immediately after acquiring a novel task, when compared to a resting period, was demonstrated. This follow-on study used the same pseudo-randomized, counterbalanced, between-subject study design, and 11 Labrador Retrievers were re-trained in the identical two-choice discrimination paradigm after a period of 1 year. The playful activities group needed significantly less trials and made significantly less errors to successfully reach the re-training criterion (Mann–Whitney U test, critical value of U at p < 0.05 is 5, U = 5, Z = 1.73, p = 0.04 and U = 4.5, Z = 1.8, p = 0.03, respectively). Following model simplification of a multiple factor/covariate general linear model analysis, the type of intervention, the number of trials needed to re-learn the task after 24 h, the average heart rate during the intervention a year ago, and age were significantly correlated to the number of trials and errors needed to resolve the task. A significant difference due to intervention allocation (heart rate during the intervention, trials needed to re-learn the task after 24 h) between the groups was confirmed. Age did not significantly differ between the groups; nevertheless, the effects of ageing cannot be fully excluded, given the low sample size. No effects of the trainer and of the cortisol concentrations (of the previous year) were observed. This is the first evidence that post-training activity may influence memory up to 1 year after task acquisition.

Assisting Decision-Making on Age of Neutering for 35 Breeds of Dogs: Associated Joint Disorders, Cancers, and Urinary Incontinence.

Neutering (including spaying) of male and female dogs in the first year after birth has become routine in the U.S. and much of Europe, but recent research reveals that for some dog breeds, neutering may be associated with increased risks of debilitating joint disorders and some cancers, complicating pet owners' decisions on neutering. The joint disorders include hip dysplasia, cranial cruciate ligament tear or rupture, and elbow dysplasia. The cancers include lymphoma, mast cell tumor, hemangiosarcoma, and osteosarcoma. In previous studies on the Golden Retriever, Labrador Retriever and German Shepherd Dog, neutering before a year of age was associated with increased risks of one or more joint disorders, 2–4 times that of intact dogs. The increase was particularly seen with dogs neutered by 6 months of age. In female Golden Retrievers, there was an increase in one or more of the cancers followed to about 2–4 times that of intact females with neutering at any age. The goal of the present study was to expand and use the same data collection and analyses to cover an additional 29 breeds, plus three varieties of Poodles. There were major breed differences in vulnerability to neutering, both with regard to joint disorders and cancers. In most cases, the caregiver can choose the age of neutering without increasing the risks of these joint disorders or cancers. Small-dog breeds seemed to have no increased risks of joint disorders associated with neutering, and in only two small breeds (Boston Terrier and Shih Tzu) was there a significant increase in cancers. To assist pet owners and veterinarians in deciding on the age of neutering a specific dog, guidelines that avoid increasing the risks of a dog acquiring these joint disorders or cancers are laid out for neutering ages on a breed-by-breed and sex basis.

Pathogenic variants in COL6A3 cause Ullrich-like congenital muscular dystrophy in young Labrador Retriever dogs

The collagen VI-related muscular dystrophies in people include a broad spectrum of diseases ranging from the severe Ullrich congenital muscular dystrophy to the mild Bethlem myopathy. Clinical features are attributable to both muscle and connective tissue and include progressive muscle weakness and respiratory failure, hyperlaxity of distal joints, and progressive contracture of large joints. Here we describe two different COL6A3 pathogenic variants in Labrador Retriever dogs that result in autosomal recessive or autosomal dominant congenital myopathies with hyperlaxity of distal joints and joint contracture, similar to the condition in people.

Risk factors for injury among military working dogs deployed to Iraq

There is limited literature concerning the types of injuries that military working dogs (MWDs) face while in a deployed theater of operations and associated risk factors. To summarize injuries and identify injury risk factors in MWDs during their first deployments to Iraq, demographic and medical data were collected for 794 MWDs from the U.S. Army, Air Force, Navy, and Marine Corps that deployed to Iraq between March 20, 2003 and December 31, 2007. Sixty-two percent (n = 490) had a medical encounter during deployment. Injuries were categorized as traumatic or musculoskeletal. MWD demographics, characteristics, and injury types were summarized. Injury risk factors were assessed using multivariable logistic regression.A majority of the population were German Shepherds (56 %), intact males (49 %), and dogs certified in both patrol and explosives detection (73 %). During their first deployment to Iraq, 20 % (n = 156) experienced an injury. Risk factors included breed, age, and occupational certification. Belgian Malinois and Labrador Retriever dogs had greater odds of injury compared to German Shepherds (p = 0.04 and p = 0.02) and the oldest MWDs had about a 50 % higher risk of injury compared to the youngest (p = 0.01), especially for musculoskeletal injuries. MWDs with Specialized Search certification were at increased injury risk (p = 0.02). Training, equipment, and supplies for veterinary service personnel, MWD handlers, and MWDs should be tailored with consideration of the injury risks of the MWD population. Further study is needed to investigate chronic injuries in military working dogs to better understand causation and prevention.

Steinmann Pins and Polymethylmethacrylate Repair of Fourth Lumbar Vertebral Fracture and Dorsocranial Luxation in PuppySteinmann Pins and Polymethylmethacrylate Repair of Fourth Lumbar Vertebral Fracture and Dorsocranial Luxation in Puppy

Background: Acute spinal traumas can lead to irreversible damage associated with vascular and inflammatory changes in neural tissue. Since spine and spinal cord traumas have an unfavorable prognosis in small animals, and reports of the use of Steinmann pins and polymethylmethacrylate repair of lumbar vertebra fracture-luxation in puppies are rare in the literature, the present paper aimed to report the surgical treatment of transversal fracture through the body of the fourth lumbar vertebra, with dorsocranial displacement of the caudal fragment by using Steinmann pins and polymethylmethacrylate in a 7-month-old Labrador Retriever male dog.Case: A 7-month-old intact male Labrador Retriever dog, weighing 24.0 kg was attended at School Veterinary Hospital with a history of hit by car and paraplegia of the hind limbs. On neurological examination was observed no proprioception and no deep pain sensitivity on the both pelvic limbs. The lumbar spinal palpation showed intense pain, and the motor function and patellar reflexes were reduced. The values of haematological and biochemical analysis remained within the reference values for the species. Radiographs revealed a transversal fracture through the body of the fourth lumbar vertebra, with dorsocranial displacement of the caudal fragment, and was decided to perform a surgical treatment by open reduction and internal fixation of the fracture. The dog was positioned in ventral recumbency for surgery, and a dorsal midline incision was made from the second lumbar vertebra to the sixth lumbar vertebra. Two crossed 1.5 mm Kirschner wires were placed through the caudal articular facets of the fourth lumbar vertebra to provide initial stability. Two 2.0 mm Steinmann pins were placed at 60° angle of the bodies of the second and fifth lumbar vertebrae, and third and fourth lumbar vertebrae. This procedure was repeated on the other side of the vertebral bodies. Sixty grams of polymethylmethacrylate bone cement was applied and the fixation was checked for stability. Muscular, subcutaneous tissue and skin was closed routinely. The dog was paraplegic without any pain and used a wheelchair to assist in locomotion, although it still had urinary and faecal incontinence; no tail control; and the implants were not removed.Discussion: Lumbar vertebral injuries, as in the present case frequently, occur secondary to severe trauma (e.g. hit by car) and seem to have a characteristic fracture pattern. The patient became paraplegic due to spinal cord injury and failure of sensitivity and elimination of urine and feces which can be associated with progressive destruction of neuronal tissue due to secondary vascular and inflammatory events. The radiographic exam was used to confirm the spinal injury and determine whether by conservative or surgical treatment. The treatment choice is related to the number of fractured compartments, and the presence or not of compression. Fractures in two or more compartments are indicative of surgical treatment, similar as the present case. Use of Steinmann pins was to make a rigid fixing system compared with block plates used for the same function. Use of polymethylmethacrylate may also become a complication due to the infection, for this reason was prescribed an antibiotic for 15 days. This procedure proved to be effective since the dog showed no signs of infection. According to the characteristics of the present case report, the use of Steinmann pins and polymethylmethacrylate for repair of transversal fracture through the body of the fourth lumbar vertebra, with dorsocranial displacement of the caudal fragment provided an effective and practical means of stabilisation, promoting decompression, and thus, improving the patient's quality of life.

Effects of 0.02% netarsudil ophthalmic solution on intraocular pressure of normotensive dogs.

To evaluate the effect of QD or BID 0.02% netarsudil ophthalmic solution (Aerie Pharmaceuticals) on intraocular pressure (IOP) in normotensive dogs and to describe any adverse effects. Normotensive Labrador retriever dogs were included in this study: 10 received netarsudil in one eye and artificial tears in the contralateral eye QD, and 10 received netarsudil in one eye and artificial tears in the contralateral eye BID. Intraocular pressure curves were acquired over a 3-day acclimation period, 5-day dosing period (QD or BID-10 dogs/group), and 3-day recovery period. Toxicity was assessed daily using slit-lamp biomicroscopy and the semiquantitative preclinical ocular toxicology scoring system. Once-daily dosing did not lower IOP over the entire 5-day dosing period (95% CI 0.1 to -0.9mmHg, P=.20) or on the last day of dosing (95% CI 0.4 to -0.9mmHg, P=.65). Twice-daily dosing resulted in a statistically significant, but clinically unimportant, IOP reduction over the entire 5-day dosing period (-0.6mmHg; 95% CI 0.05 to -1.1mmHg, P=.02) and on the last day of dosing (-0.9mmHg; 95% CI 0.2 to -1.5mmHg, P=.003). Adverse events were limited to transient mild-to-moderate conjunctival hyperemia during the dosing phase in eyes receiving netarsudil vs control (P<.0001). Netarsudil 0.02% ophthalmic solution twice daily resulted in a small, statistically significant, but clinically unimportant, IOP reduction in normotensive dogs. Future studies should investigate efficacy in glaucomatous dogs.

The effects of breed, age, sex, and body weight on electrocardiographic parameters in military working dogs

Aim:This study aimed to investigate the effects of breed, sex, age, and body weight on different electrocardiographic parameters in military working dogs (Labrador Retriever and German Shepherd).Materials and Methods:Electrocardiographic recordings (paper speed = 25 mm/s and calibration = 10 mm/mV) were performed to obtain all the standard bipolar limb leads (leads I, II, and III) and unipolar augmented limb leads (leads aVR, aVL, and aVF). A total of 16 Labrador Retrievers and 14 German Shepherds were restrained manually in the right lateral recumbency without any tranquilizer or anesthetic drug. Amplitude and duration of P, QRS, and T wave, PR and QT interval, mean electrical axis, and heart rate were measured in each recording.Results:There was no significant difference in electrocardiographic parameters across breed and sex. However, QRS duration tended to alter by breed (p<0.1) in Labrador Retrievers (0.04±0.005 s), which is lower than German Shepherds (0.05±0.005 s). PR interval was influenced by sex (p<0.1). PR interval was higher in females (0.13±0.005 s) than males (0.11±0.008 s). In addition, electrocardiographic values were not significantly affected by age and body weight, except that the amplitude of R wave was statistically affected by age (p<0.05). A correlation was found between the decrease in R wave amplitude and increase in age of dogs.Conclusion:Different electrocardiographic parameters were within the normal range. A significant effect of age was seen on amplitude of R wave. However, the effect of breed, sex, and body weight was not significant on different electrocardiographic parameters in Labrador Retriever and German Shepherd dogs.

Renal failure in a Labrador Retriever dog – A case study

Renal failure in a Labrador Retriever dog – A case study