Simple SummaryAlthough mechanisation has markedly reduced animal labour demand in agriculture, draught animals are still used in small production units located on terrains that do not favour agriculture mechanisation. Especially in Africa, Latin America, and Asia, they represent one of the main sources of sustenance for thousands of families who utilise animal labour in numerous agricultural tasks, such as ploughing and harvesting, as well as means of transport and hauling. Depending on the geographic area, the species involved are equids (horses, donkeys, and mules) and bovids (buffaloes and cattle). Draught animals proved to be sustainable in terms of global warming and the use of non-renewable energy as compared with agricultural machinery. However, critical points are the quality of human–animal interaction, due to the close contact between animals and humans while working, and the welfare of draught animals when transported and slaughtered, due to the high prevalence of injuries they suffer when subjected to these practices. Therefore, their use should be promoted in rural marginal areas where only low investments are usually feasible, and the energy of the animals can be obtained at a low cost by feeding them harvest residues and by-products.This study discusses scientific findings on the use of draught animals such as equids (i.e., horses, mules, and donkeys) and bovids (i.e., cattle and water buffaloes) in rural labours. Relevant peer-reviewed literature published between 1980 and 2021 was retrieved from CAB s, PubMed, ISI Web of Knowledge, and Scopus databases. Although animals were used to produce draught power since their domestication and are still being used for this purpose, mechanisation has markedly reduced animal labour demand in agriculture. However, the process was uneven across continents according to economic constraints, and draught animals are currently concentrated in small production units located on terrains that do not favour agriculture mechanisation in Africa, Latin America, and Asia. Generally, equids can work at rates similar to those of bovids or faster but can sustain the work for shorter periods of time. In addition, buffaloes possess tough hooves and resistance to disease that make them suitable for working in wetlands and clay soils. Draught animals allow a marked reduction of both GHG emissions and non-renewable energy consumption as compared with agricultural machinery. In addition, they may allow obtaining profits from otherwise non-usable lands. Therefore, their use should be promoted in rural areas where low investments are usually the only ones feasible, and the energy of the animals can be obtained at a low cost by feeding them harvest residues and by-products. However, more attention should be paid to the quality of human–animal interactions—due to the close contact between animals and humans while working—and to the welfare of draught animals when transported and slaughtered—due to the high prevalence of injuries they suffer when subjected to these practices.