White Laboratory Rats Research Articles

The role of the tuftsin and Selank peptides in the regulation of primary and plasma homeostasis

Experimental data on the effect of the natural immunopeptide tuftsin and its synthetic counterpart on hemostasis parameters in vitro and in vivo when administered intranasally to animals (white laboratory rats) are given. It has been shown that in vitro both preparations induced a fibrin-depolymerization effect on fibrin that is not stabilized by factor XIIIa. For each preparation, it was noted that, when administered to rats, simultaneous antiplatelet, anticoagulant, and fibrin-depolymerization effects were observed in the blood plasma, with Selank having a more pronounced effect.

Endogenous ethanol and acetaldehyde in the mechanisms of adaptation of small mammals to northern conditions

Study results on the condition of endogenous ethanol—acetaldehyde system in northern red-backed vole (Myodes (=Clethrionomys) rutilus Pallas, 1779), common species of northern latitudes, are presented. Concentrations of endogenous ethanol (EE) and acetaldehyde (EA) in blood, activities of alcohol dehydrogenase in ethanol oxidation reactions (ADHI) and liver acetaldehyde (ADHII) and aldehyde dehydrogenase (ALDH) reduction, and catalytic parameters of these enzymes are determined for winter and summer periods. Changes in ethanol—metabolizing dehydrogenase enzyme system in northern red-backed vole leading to statistically significant (p < 0.01) increase in blood EE and EA concentrations compared to white nonpedigreed laboratory rats without adaptations to cold (especially in winter period) are determined to serve ecoadaptive, antistress functions, comprising one of the systems for regulation of metabolism intensity in small mammals inhabiting extreme northern conditions. Ecological—physiological functions of EE and EA system are increasing the organism’s resistance to the influence of cold temperatures and maintaining thermoregulation even under rapid, considerable environmental temperature drops.

The effect of hydroalcoholic extract of Mentha piperita on pentylenetetrazol-induced convulsion in mice

Background: Convulsion is one of the most important disorders of the central nervous system. On account of their lower side effects, the use of medical herbs for treating diseases, including convulsion, is on the rise. Aims and Objectives: The present study aimed at investigating the effect of peppermint extract on pentylenetetrazol (PTZ)-induced convulsion in small white (laboratory) rats. Materials and Methods: This study was conducted on 64 mice. The treatment groups received various (400, 600, and 800 mg/kg) doses of hydroalcoholic peppermint extract. The positive and negative control groups received Diazepam (1 mg/kg) and normal saline (10 ml/kg)30 min before intraperitoneal PTZ injection (85 mg/kg), respectively. Such factors as the onset, length, and severity of convulsion were then studied. To determine the best extract dose, the response times for min 15, 30, 45, and 60 before PTZ injection were studied. Results: Results from the administration doses of the extract revealed that it had a dose-dependent effect in that no convulsion was seen at 800 mg/kg. To investigate the effect of interval between appropriate extract dose injection (800 mg/kg) on PTZ levels, 15-, 30-, 45-, and 60-min intervals were chosen. Results showed that the best result of the 800 mg/kg extract dose was achieved 30 min before PTZ injection where no convulsion was observed. Conclusion: The overall result revealed that peppermint extract had preventive effects on PTZ-induced epileptic attacks in mice where the 800 mg/kg dose brought about the best result, i.e., no convulsion.

CHARACTERISTICS OF METABOLISM AND WORKING OUT THE METHODS OF DETERMINATION OF SYNTHETIC CANNABINOID THJ-2201 IN THE URINE OF LABORATORY ANIMALS

The proposed article touches upon the study of metabolism of new synthetic cannabinoids. In the work the data on synthetic cannabinoid 3-(Naftalin-1-yloxomethyl)-1-(5-fluoropentyl)-1H-indazole (THJ-2201), as well as the products of its metabolism in the laboratory rats of the Wistar line are given, i. e. Mass spectra and chromatograms of the native substance (THJ-2201) obtained by high-performance liquid chromatography with mass-selective detection (HPLC-MS) and gas chromatography with mass-selective detection (GC-MS) are given. The paper presents a complex technique for qualitative determination of cannabimimetics THJ-2201 and methods for obtaining a metabolic profile model for the test substance that can be useful for the tasks of qualitative detection and detection of new psychoactive substances in biological objects for the purposes of forensic analysis. The aim of this work was to develop methods for determination of the test substance (THJ-2201) and its metabolites in the urine of laboratory animals, as well as the study of the metabolic characteristics of synthetic cannabinoids on the whole. Materials and methods. The following equipment was used for the experiment: a liquid chromatograph from the firm “Shimadzu LCMC-8050” in combination with a mass-selective detector. The detector type is a triple quadrupole with a double ionization source (chemical ionization at atmospheric pressure and electrospray). The separation of the substances occurred in a chromatographic column (the material is stainless steel, the characteristics are: 150 * 3.0 mm, Luna 3uC18 (2), 100A). The Sorbent is reversed-phase. The investigations were carried out on Agilent 7890A gas chromatograph with Agilent 5975C mass spectrometer and a 103 polar HP-5ms column of 28 m × 0.25 mm. The animals were mature male white laboratory rats of the Wistar line, aged 4–6 months, weighing 190-230 grams. Results and discussion . As a result of the studies, a comprehensive methodology for determining the synthetic cannabimimetics of THJ-2201 and its metabolites in the urine of laboratory animals was developed, chromatograms and mass spectra of the metabolic profile of animals were obtained by HPLC-MS and GC-MS methods, and the similarity of the metabolic profiles of the studied substances in humans and animals was determined. Conclusion . The conclusions were made about the suitability of the proposed complex analysis methodology under the given laboratory conditions and the significant degree of interspecific similarity of human metabolic profiles and laboratory rats’ for substances chemically close to the studied.

The bioimpedance analysis of a parenchyma of a liver in the conditions of its extensive resection in experiment

The liver failure which is result of disharmony of functionality of a liver to requirements of an organism is the main reason for unsatisfactory results of an extensive resection of a liver. However, uniform effective criterion of definition of degree of a liver failure it isn’t developed now. One of data acquisition methods about a morfo-functional condition of internals is the bioimpedance analysis (BIA) based on impedance assessment (full electric resistance) of a biological tissue. Measurements of an impedance are used in medicine and biology for the characteristic of physical properties of living tissue, studying of the changes bound to a functional state and its structural features. In experimental conditions we carried out an extensive resection of a liver on 27 white laboratory rats of the Vistar line. The comparative characteristic of data of a bioimpedansometriya in intraoperative and after the operational period with the main existing methods of assessment of a functional condition of a liver was carried out. By results of the work performed by us it is possible to claim that the bioimpedance analysis of a liver on the basis of an invasive bioimpedansometriya allows to estimate morphological features and functional activity of a liver before performance of an extensive resection of a liver. The data obtained during scientific work are experimental justification for use of an impedansometriya during complex assessment of functional reserves of a liver. Preliminary data of clinical approbation at a stage of introduction of a technique speak about rather high informational content of a bioimpedansometriya. The subsequent analysis of efficiency of the invasive bioimpedance analysis of a liver requires further accumulation of clinical data. However even at this stage the method showed the prospect for further use in clinical surgical hepathology.

Histomorphometric analysis of stomach walls alternation under conditions of general body dehydration.

Background. Changes of the composition of food and beverages intake by modern humans do not fulfill the nutritious, energetic and water needs of the body. This causes rapid growth of gastrointestinal diseases. The stomach is particularly susceptible to the influence of external factors. Water-salt metabolism disorders cause structural and functional alternations of the stomach as one of the digestive organs. Objective. The present study was conducted to identify changes of the fundus of the stomach wall of the rats under condition of general body dehydration of different severity level. Methods. The object of this research was a group of 29 adult white male laboratory rats with average weight of 150-170 g. The control group comprised of 7 rats which had the usual water intake in the course of the experiment. Light dehydration occurred on the 3rd day of the experiment, medium dehydration happened on the 6th day and severe dehydration – on the 9th day. The animals were sacrificed on the days stated above by means of euthanasia with anesthetic ethers. Sampling and fixation were carried out in accordance with the established procedures. To examine the structural components the histologic sections were stained with hematoxylin and eosin stain, using Van Gieson's staining and Mallory staining. Morphometric analysis included defining of the thickness of stomach walls of the animals of all the groups. Results. On the 3rd day from the experiment launch (mild dehydration) there was observed a fair decrease in the average layer thickness of the surface epithelium as well as other components of the stomach walls as compared to the results of the intact group of animals. Mallory staining determined a major thinning of the mucous layer on the surface of the gastric mucosa as well as mucus presence in cytoplasm of mucous cells. On the 6th day of the experiment (moderate dehydration) the average layer thickness of surface epithelium decreased compared to the control group. There was a tendency of thinning of the stomach wall thickness and all of its structural components as compared to the intact animal group. Mucous membrane had erosions which looked like surface defects on the gastric folds. On the 9th day of the monitoring (severe dehydration) a considerable decrease of thickness index of all of the gastric wall components. This was accompanied by irreversible dystrophic and necrobiotic cell changes mainly in the gastric glands necks and bodies which lead to total cytolysis. There is reported moderate vascular blood congestion, haemorheologic disorders which manifest themselves as stasis and thrombosis of microcirculatory bloodstream vessels. Conclusion. The obtained results indicate thinning of stomach walls, irreversible dystrophic changes and partial loss of gland cells function as well as local circulation failure and haemorheologic changes in microvascular vessels which progress from mild to severe general dehydration.

Стимуляция аутогенным костным мозгом остеорепарации в зоне смоделированного ложного сустава бедренной кости у крыс

Purpose To study the morphological features of bone regeneration by using a local injection of autologous red bone marrow in the conditions of an experimentally produced pseudarthrosis. Materials and methods The study was conducted on thirty (30) white laboratory rats that were bred at the Sitenko Institute for Spine and Joint Pathology of the Ukraine National Academy of Medical Sciences (6 months old, weight of 250-270 g). The experiment was conducted according to the international rules of experimental animals protection. The protocol of the experimental studies was approved by the ethics board of the Sitenko Institute for Spine and Joint Pathology of the National Academy of Medical Sciences of Ukraine. Results Culturing of mesenchymal stromal cells requires high-tech laboratory equipment and takes a long period of time. Therefore, their widespread clinical use is limited. Native bone marrow that contains mesenchymal stromal cells is easily available. The process of its preparation and transplantation is not technologically difficult. Therefore, it has advantages for being used for stimulation of reparative osteogenesis in bone fractures or in disorders of reparative osteogenesis. Conclusion The study found that autologous red bone marrow stimulated bone tissue development in the zone of pseudarthrosis. The area of bone tissue was 22.8 % larger as compared with the bone area in the untreated animals. There were no pseudarthrosis-related cavities in the regenerate structure in the cases of red bone marrow injection into the zone of traumatic injury. The features of reparative process were the following: angiogenesis activation, enchondral ossification accompanied by cancellous bone tissue formation with a network of bone trabeculae, and decrease in the destructive manifestations in the maternal bone parts located above and below the injury area.

Особливості структурної перебудови шлунка та їх морфометрична характеристика після видалення великих слинних залоз у щурів

Дослідження проведено на лабораторних білих щурах-самцях, яких було поділено на 2 групи: контрольну й експериментальну. Експериментальній групі тварин проводили одномоментну двобічну сіаладеномектомію привушних і нижньощелепних залоз. Результати проведеного дослідження свідчать про те, що після одномоментного двобічного видалення привушних і нижньощелепних залоз на тлі гіпосалівації в структурних елементах стінки шлунка щурів відбувається відчутна структурна реорганізація, яка полягає у прогресуючому розвитку дистрофічно-атрофічних процесів у слизовій оболонці шлунка з метапластичною трансформацією її залозистого епітелію та одночасними гіпертрофічно-гіперпластичними змінами м’язової оболонки.

Peculiarities of hormonal and metabolic changes in offspring of the first generation from rats internally exposed by 131I

В експерименті досліджено віддалені біологічні ефекти у нащадків першого покоління білих лабораторних щурів, які зазнали впливу інкорпорованого 131І. Визначено дискоординацію у функціонуванні гіпофіз-тиреоїдної ланки ендокринної регуляції, посилення процесів ліпопероксидації, порушення ліпідного обміну та про-антиоксидантної рівноваги

Features of cells and extracellular matrix ratio in rat meniscus for motor activity formation during the first two weeks of postnatal life in norm and after intrafetal antigen injection.

Background. Diseases of the musculoskeletal system and connective tissue are included in the most common among school-age children. Undifferentiated connective tissue dysplasia syndrome is the morphologic basis for their progress. Previous researches showed that intrafetal antigen injection can be used for its modeling. Reactivity of the knee joint meniscus is not studied in this condition. Objective. To establish the features of the dynamics of cells and extracellular matrix ratio in the menisci of rat knee joint in norm and after intrafetal antigen injection from the 1st to the 14th day of life. Methods. 80 white laboratory rats were studied. 30 rats were intact. 30 rats underwent transuterine, transmembranous, intrafetal injection of liquid purified staphylococcal anatoxin (10-14 binding units in 1 ml, tenfold diluted, 0.05 ml) on the 18th day of prenatal period according to the method of M.A. Voloshyn. 20 rats after injection of saline solution served as control. Paraffin sections of left knee joint were made and stained with alcian blue (pH 2,6, MgCl2 critical concentration 0,2 M). In the sections the relative area of cells and matrix in the inner and outer zones of medial and lateral meniscus were measured. Zones were divided according to their cells types: fibroblast-like cells in the outer zone and chondrocyte-like cells in the inner one. Results were considered significant at p &lt; 0.05. The differences between the averages were evaluated using Student's t-test. Results. On the 1st day of experiment in both zones of the medial meniscus the relative area of cells increases as compared to the norm. During two weeks it decreases, except for the day 11th when it is significantly more in the outer zone. In the inner zone of the lateral meniscus cells relative area is less on the 11th day of the experiment, but more on the 1st and 14th. During the 1st week of postnatal life in the experiment the amount of extracellular matrix in the outer zone is significantly higher than in norm, and later, during the second week, it was not significantly different. Conclusion. It was established that the prenatal action of antigens leads to the disorder of the rate and terms of morphogenesis during the formation of motor activity, which is manifested by the changes in the ratio of cells and matrix as compared to the norm.

Preclinical Pharmacokinetic and Pharmacodynamic Studies of Pharmaceutical Compositions of the Dipeptide Anxiolytic GB-115

Four pharmaceutical compositions for oral use that contained the dipeptide anxiolytic GB-115 as the active ingredient underwent preclinical trials in male laboratory white rats. Various excipients added to the compositions and their preparation technologies had significant effects on the dipeptide pharmacokinetics. It was shown that compositions 2 and 4 had advantages according to the main pharmacokinetic characteristics reflecting the bioavailability of GB-115. The pharmacological activities of GB-115 compositions 1 – 4 at oral doses of 0.3 – 0.9 mg/kg were studied in an elevated plus-maze (EPM) test. It was established that composition 4, which included micronized substance and a hydrophilic matrix with controlled release of the active ingredient (hydroxypropylmethylcellulose), possessed the highest anxiolytic activity and increased the residence time in the EPM open arms at doses of 0.3 mg/kg (p < 0.01) and 0.9 mg/kg (p < 0.01) as compared with the placebo group. Pharmaceutical composition 4 could be recommended for creating a controlled-release solid dosage form based on the comprehensive studies.

Особливості просторової і структурної організації шлунка білих лабораторних щурів у нормі

&lt;p&gt;Резюме. Дослідження проведено на 12 інтактних білих лабораторних щурах. Макрометрично вимірювали довжину, ширину і товщину шлунка з наступним обчисленням його об’єму. Для гістологічного дослідження забирали шматочки тканин із різних відділів шлунка. Морфометричні дослідження включали визначення товщини слизової, м’язової, серозної оболонок та підслизової основи, площі епітеліоцитів, діаметра ядер епітеліоцитів, ядерно-цитоплазматичних співвідношень (ЯЦС). Середні розміри шлунка у інтактних тварин: довжина – (3,93±0,13) см, ширина – (2,20±0,09) см, товщина – (1,45±0,08) см, об’єм шлунка у середньому складає (6,72±0,81) см³. Найбільший питомий об’єм має дно шлунка. При зовнішньому макроскопічному огляді дно шлунка за його білуватим забарвленням легко можна відрізнити від червоно-сіруватих тіла та пілоричної частини. Ще чіткіше дно шлунка можна віддиференціювати при візуальному огляді його слизової оболонки: слизова оболонка дна відмежована від тіла шлунка чіткою дугоподібною складкою або складчастим краєм. При гістологічному дослідженні складчастого краю встановлено, що його основу складає дуплікатра підслизової основи, яка зі сторони дна шлунка покрита багатошаровим плоским епітелієм, а зі сторони тіла – залозистим епітелієм. Морфометричні дослідження стінки шлунка інтактних білих лабораторних щурів дозволили встановити наступні кількісні параметри її структурних компонентів. Товщина серозної оболонки у середньому складала (7,83±0,31) мкм, м’язової – (40,17±0,75) мкм, підслизової – (24,00±0,78) мкм і слизової – (96,00±0,97) мкм. Площа ядер гладком’язових клітин (ГМК) дещо більша, ніж площа ядер залозистого епітелію, водночас питомий об’єм цитоплазми у ГМК, навпаки, менший. Відповідно до цього і показник ЯЦС у ГМК вищий, ніж у епітеліоцитів слизової.&lt;/p&gt;&lt;p&gt; &lt;/p&gt;&lt;p&gt;Ключові слова: шлунок щура, будова, морфометрія.&lt;/p&gt;

Preparation and Bioavailability Evaluation of Micronized Steroidal Mecigestone Drug Substance

Microcrystals of 6α-methyl-16α,17α-cyclohexapregn-4-ene-3,20-dione (mecigestone) that differed from the starting drug substance by decreased sizes and altered morphologies were produced using a Nano Spray Dryer B-90 (Buchi, Switzerland) that allowed operation with organic solvents in a closed cycle. The bioavailability of starting crystalline and micronized mecigestone drug substance was studied by oral administration to laboratory white rats. Mecigestone was extracted from rat blood serum using solvent extraction. The extraction coefficient was 64%. The detection limit of the drug substance was 0.08 ng/mL. The content of unaltered mecigestone in the extracts was determined using HPLC with high-resolution mass spectrometric detection. Micronized mecigestone was 7.5 times more bioavailable than the starting drug substance.

The Investigation of the protective effects of hydro-alcoholic extract of sea buckthorn (Hippophaerhamnoides L.) in spermatogenesis of rat after exposure of Wi-Fi radiation

Male infertility is a distressingly common condition affecting about 1 in 20 of the male population. In a majority of the cases, the male partner produces sufficient numbers of spermatozoa to achieve fertilization but there are functional defects in these cells that prevent conception from occurring. In this study, the effects of Wi-Fi radiation on spermatogenesis, testes and testosterone of large white male rats were studied, and also hydro alcoholic extract of sea buckthorn fruit were used as a protective agent against the effects of Wi-Fi radiation, and the comparative study was done . For this study 60 white male laboratory rats weighing 200-250 g were selected then tested in good conditions of light and food. The mice were randomly divided in terms of Wi-Fi Radiation and received gavage Sea buckthorn into 6 out of groups of 10.It was found that in the groups that had received Wi-Fi radiation, waves did not affect sperm count, testes weight, testosterone and testicular tissue, but it caused a significant decrease in fertilized sperm motility, and sea buckthorn extract could work as a protective agent against the radiations. According to the results of this research, it can be the probability that Sea buckthorn extract, having antioxidant substance can prevent destructive effects of waves that cause free radicals and having vitamin E improves sperm quality.

Fetal Nerve Cell Transplantation in Early Post-Resuscitation Period in Rats.

IntroductionFetal cell transplantation is a promising biomedical approach for disease treatment; however, the use of fetal cell therapy is still experimental. This research was deemed a necessity to provide evidence-based research for the application of cell transplantation as a treatment method. The aim of this study was to evaluate the effect of fetal nerve cell transplantation in rat survivors (and non-survivors) after clinical death by mechanical asphyxia.Methods68 white laboratory rats were divided into two groups of identical age and sex: a control group of 12-month adult male rats (n = 26) and an experimental group (n = 42). Rats were fixed under ether anesthesia. We then blocked the oral and nasal regions with cotton wool soaked in saline solution. A four-minute clinical death though acute mechanical asphyxia was simulated by applying the method of N. Shim. After the 4-minute clinical death, we resuscitated the rats using external cardiac massage and artifical respiration. Suspension of the fetal nerve cells was injected intraperitoneally at 1mm3 per 25g at the time of cardiac activity restoration. Lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) levels were examined in the homogenate cerebral cortex of reanimated animals. We recorded the survival rate during the post-resuscitation period and analyzed the integrative brain functions using anxiety-phobic status and latent inhibition.ResultsAfter fetal nerve cell transplantation, the enzymatic reactions in the experimental group became normal with a significant decrease in LDH and an increase in CPK levels compared to the control group. In the control group, 10 rats died and 16 lived (62% survival rate), while 7 rats died and 35 lived (83% survival rate) in the experimental group during the first 7 days. Rats that did not receive the treatment tended to die sooner than those in the experimental group. As a result of transplantation, the anxiety level in the experimental group was less than in the control group. Moreover, cell therapy improved the reflexes in the experimental animals.ConclusionsThe study revealed the positive neuroprotective effect of the fetal nerve cells on the recovery in the early post-resuscitation period. This was confirmed by the normalization of enzymatic reactions, improvement reflective activity, and increase in the survival rate of the resuscitated animals in the group treated with fetal nerve cell transplantation. These findings warrant future research on the mechanisms associated with reflex improvement.

Impact peculiarities of long-term gamma-irradiation with low-dose rate on the development of laboratory rats and their sperm production

To study the influence of long-term gamma-irradiation with low dose rate (13×10-6 cGy/s) on the body, testes and ventral prostate, development of laboratory rats, as well as quantitative parameters of sperm production along with kinetic characteristics of spermatozoa. The experiments were performed on laboratory white rats of 2,5 months in age. Animals were irradiated in gamma-field of "Ethalon" device in a dose range 0.1-1.0 Gy. Testicles, epididymises, ventral prostate were retrieved from decapitated animal, each organ weight being determined for every exposure dose. Sperm quantities in testicles and epididymises were identified with aid of phase-contrast microscopy after tissue homogenization in saline containing Troton X-100 and NaN3. Kinetic characteristics of spermatozoa were analyzed by video recording at 37 ºC. The long-term gamma-irradiation with low dose rate was shown to cause no effect on the dynamics of animal weight and weight of epididymis changes. However the testes weight was noticed to diminish at doses 0.1, 0.3, 0.6 and 1.0 Gy, the latter dose being stimulative for the ventral prostate growth and weight accumulation. Total sperm quantities in testicles and epididymices along with daily sperm production declined in gamma-irradiated rats compared to control. However curvilinear and straight line spermatozoid velocity as well as the frequency of tail oscillations tended to increase. Long-term gamma-irradiation of rat whole body with low dose rate just insignificantly affects the development of testes and ventral prostate. Apart from this, radiation effects showed up in sperm production slight suppression, from the on hand, and sperm velocity along with tail oscillations intensification, from the other hand.

The role of bile acids in morphological changes оf gastric mucosa in rats

The present study was conducted on 60 white male laboratory rats in order to investigate the role of bile acids in morphofunctional changes of gastric mucosa in rats with due regard to exposure of bile and its concentration. It was found that in the early stages of duodenogastric reflux in experiment bile in low concentrations carries protective adaptive-trophic function and promotes cell renewal of the surface epithelium. At the same time long-term exposure to the bile acids regardless of its concentration can lead to the significant dystrophic changes in gastric mucosa, pilorization and atrophy of the gastric glands. In the case of the oral administration of bile followed by «immobilization and cold stress» for one hour (I group) experiment’s results can confirm that the combined effects of several aggressive factors lead to the more severe pathological changes in the gastric mucosa: first erosive and later ulcerative lesions.

PNA- and SBA-positive lymphocytes content in the major salivary glands’ structures during early postnatal period after intrauterine antigenic action

Aim – to determine the Peanut agglutinin- and Soybean agglutinin-positive (PNA+, SBA+) lymphocytes content in the major salivary glands’ structures in early postnatal period after intrauterine antigenic action.Methods and results. 224 submandibular salivary glands of white laboratory rats were investigated through the lectinhistochemical and statistical methods.Conclusions. In newborn animals after intrauterine antigen action the increased content of PNA+ intraepithelial lymphocytes and PNA+ stromal lymphocytes of major salivary glands was determined as compared to control group. That tendency remains till the 14th day, gradually decreasing till the 45th day of postnatal formation. Quantity of SBA+ lymphocytes decreased in antigenpremium animals as compared with control group animals. In addition, the quantity of SBA+ lymphocytes in experimental group remains lower during two weeks after birth. On the 45th day ofpostnatal formation the findings do not differ among experimental animals.

Морфолого-статистическая характеристика взаимодействия периферических эндокринных желез в экспериментальной модели отдаленных последствий γ-облучения

A promising approach for objectification of the results of research is experimental simulation of conditions of influence of radiation factors, with the subsequent processing of data using new computer technologies and programs, statistical and mathematical analysis. Experiment was made on laboratory white rats males (351 individuals) in the conditions of single and fractioned γ - radiations in doses 5, 10, 25, 50 mZv with a power of 50 sGr / h The objects of study are the thyroid gland and beam zone adrenal cortex. The criterias of morphological and functional state of peripheral endocrine glands are estimated after 12, 18, 24 months of exposition of the specified doses γ- radiations by means of complex of general histological, special, histochemical, statistical methods and mathematical modeling of the influence parameters. Modeling of experimental conditions the radiation damage under γ-irradiation in the range of doses allowed to conducting morphological and statistical analysis of remote consequences of exposure and to reveal data about the causality of interaction of the thyroid gland and beam zone adrenal cortex, with the increasing correlation in prolongation of post timing observations. This shows the increase in direct proportion to the effect of defeat on the background of the formation of neoplasms of the thyroid gland.

Regeneration of bone tissue under implantation of sapphire with titanium coating

Hip replacement is the most effective method of treatment of coxarthrosis of stages III–IV allowing patients to improve quality of life and to restore the ability to work. However, term of service of the prosthesis is limited. Objective: To study regeneration of bone tissue using sapphire implants with titanium coating. Methods: Samples of pure titanium coated sapphire implanted into the distal metaphysis of the femur to 9 white laboratory rats, and without any coating — to 9 animals. At 7, 14 and 30 days after surgery the area of implantation were examined by standard methods of histology with morphometry. Results: Microscopic analysis of zone of the implantation of samples showed the formation of high-grade bone regeneration according to stage-temporal characteristics of reparative osteogenesis. After 7 days connective tissue and young bony trabeculae with high density of osteoblasts on the surface formed around implants. After 14 days in the animals of both groups there was mature connective tissue in the area of defect. The newly formed bone trabeculae in some places fitted snugly to the area of implantation. On the 30th day around zone of implantation we revealed mature bone tissue adherent to the maternal bone. In some areas between the bone and the implant we determined a thin layer of connective tissue. There were not found any toxic effects of the materials used. Morphometric analysis revealed a greater (to 1.99 times) area of bone regeneration around titanium coated sapphire implants compared to uncoated samples. Conclusions: High osteoinductive properties of titanium after implantation into the distal metaphysis of the femur of the rats were confirmed. However, the presence of the connective tissue layer around the testing samples with coating and without it does not allow to say about appearance of a complete bony fusion between the bone and the implant.