Laboratory Survey Research Articles

Harmonization of criteria and terminology in fetal rat skeletal evaluations.

A survey of laboratories in North American and Europe that routinely conduct fetal skeletal examinations was performed with the purpose of (1) understanding current terminology used for classifying skeletal findings in developmental toxicity (DT) studies and (2) understanding the criteria used to identify relatively common findings that sufficiently deviate from normal. The goal was to promote terminology harmonization and improve interlaboratory consistency in the criteria used to identify developmental anomalies. The survey, designed based on terminology for developmental anomalies recommended by an international collaboration (Makris et al., Congenital Anomalies, 2009;49(3):123-246), was conducted by a subgroup (authors of this publication) of the Royal Society of Biology's International Register of Fetal Morphologists (IRFM). Individual and summarized anonymized responses are provided here. The authors, who are expert fetal morphologists with experience performing fetal examinations, reviewed the responses and generated recommendations on preferred terminology and criteria for determining when morphological variations deviate from normal and warrant recording of the findings for skeletal observations in Sprague Dawley (SD) fetal rats. The objective of these recommendations is to complement Makris et al. (Congenital Anomalies, 2009;49(3):123-246). The broad application will improve interlaboratory harmonization of recording fetal skeleton findings in developmental toxicity studies intended for regulatory submissions, including SEND (Standard for Exchange of Nonclinical Data).

Subjective social status, race, and metabolic syndrome in women with breast cancer.

To evaluate the association of subjective social status (SSS) with metabolic syndrome (MetS) severity and its potential contribution to racial health disparities in women with breast cancer. Multicenter cross-sectional study (10 US hospitals) in women (n = 1206) with primary diagnosis of invasive breast cancer received during Mar/2013-Feb/2020. Participants, self-identified as non-Hispanic White or Black, underwent physical and laboratory examinations and survey questions assessing socioeconomic parameters, medical history, and behavioral risks. SSS was measured with the 10-rung MacArthur scale. MetS severity was measured with a validated Z-Score. Generalized linear mixed modeling was used to analyze the associations. Missing data were handled using multiple imputation. Average age was 58years. On average, the SSS of Black women, given equivalent level of income and education, was lower than the SSS of White women: 6.6 (6.1-7.0) vs 7.7 (7.54-7.79) among college graduates and 6.8 (6.4-7.2) vs 7.6 (7.5-7.8) among women in the high-income category (> $75,000). In multivariable analysis, after controlling for age, income, education, diet, and physical activity, increasing SSS was associated with a decrease in MetS-Zscore, -0.10 (-0.16 to -0.04) per every 2 rung increase in the MacArthur scale. Black women with breast cancer rank their SSS lower than White women with breast cancer do at each level of income and education. As SSS is strongly associated with MetS severity these results identify potentially modifiable factors that contribute to racial disparities.

CRISPR/Cas13a-assisted rapid and portable HBV DNA detection for low-level viremia patients

ABSTRACT Background & Aims The WHO declared to eliminate hepatitis B virus (HBV) by 2030. However, an increasing number of patients are presenting with low-level viremia (LLV) with the widespread use of antiviral medications. The diagnostic efficiency and coverage area of HBV infection are low. Hence, this study intended to drive the HBV infection detection to effectively adaptable for any small to medium-sized laboratory or field survey. Methods We established, optimized, and evaluated a colloidal gold test strip for detection of HBV DNA based on CRISPR/Cas13a combined with recombinase-aided amplification (RAA) technology. Furthermore, 180 HBV-infected patients (including patients with different viral loads, LLV patients and dynamic plasma samples of patients on antiviral therapy) were enrolled for clinical validation. Results The strip detection of HBV DNA was established based on RAA-CRISPR-Cas13a technology with a sensitivity of 101 copies/μL and a specificity of 100%. HBV DNA gradient concentration plasmids and clinical samples were effectively identified by this approach. The positive coincidence rate for LLV patients was 87%, while the negative coincidence rate was 100%. The positive coincidence rate reached 100% in LLV patients (viral loading >100 IU/mL). The sensitivity, specificity, positive predictive agreement (PPA) and negative predictive agreement (NPA) values of dynamic plasma detection in patients on antiviral therapy were 100%, 92.15%, 93.75%, and 100%, respectively. Conclusions We develop rapid and portable RAA-CRISPR/Cas13a-based strip of HBV DNA detection for LLV patients. This study provides a visual and faster alternative to current PCR-based diagnosis for HBV infection.

Same Question, Different Answers?

Abstract: Psychological scientists increasingly study web data, such as user ratings or social media postings. However, whether research relying on such web data leads to the same conclusions as research based on traditional data is largely unknown. To test this, we (re)analyzed three data sets, thereby comparing web data with laboratory and online survey data. We calculated correlations across these different data sets (Study 1) and investigated identical, illustrative research questions in each data set (Studies 2–4). Our results suggest that web and traditional data are not fundamentally different and usually lead to similar conclusions, but also that it is important to consider differences between data types such as populations and research settings. Web data can be a valuable tool for psychologists when accounting for such differences, as it allows for testing established research findings in new contexts, complementing them with insights from novel data sources.

Neurocognitive and psychiatric symptoms following infection with COVID-19: Evidence from laboratory and population studies

ObjectiveThe objective of the current investigation was to examine associations between symptomatic COVID-19 history, neurocognitive function, and psychiatric symptoms using cognitive task performance, functional brain imaging, and a prospective population survey. MethodsStudy 1 was a laboratory study conducted between 3 May 2022 and 16 Nov 2022 involving 120 fully vaccinated community dwelling adults between 18 and 84 years of age (Mage = 31.96 (SD = 20.71), 63.3% female). In this cross-sectional study we examined the association between symptomatic COVID-19 infection history and performance on three computer tasks assessing cognitive function (Flanker interference, delay discounting and simple reaction time) and measured oxygen saturation within the prefrontal cortex using functional near infrared spectroscopy (fNIRS). Study 2 was a 2-wave population survey undertaken between 28 September 2021 and 21 March 2022, examining the prospective relationship between symptomatic COVID-19 and self-reported symptoms of cognitive dysfunction, depressive symptoms, anxiety symptoms, and agitation at 6-month follow up. The sample (N = 2,002, Mage = 37.0, SD = 10.4; 60.8% female) was collected using a quota process to ensure equal numbers of vaccinated and unvaccinated individuals. Structural equation modelling with latent variables was performed on the population-level data, evaluating the fit of the proposed mediational model of symptomatic COVID-19 to psychiatric symptoms through cognitive dysfunction. ResultsFindings from Study 1 revealed significant effects of symptomatic COVID-19 history on Flanker interference and delay discounting. Effects on flanker performance were significantly stronger among older adult women (effect: 9.603, SE = 4.452, t = 2.157, p = .033), and were accompanied by task-related changes cerebral oxygenation at the right superior frontal gyrus (F (1, 143.1) = 4.729, p = .031). Additionally, those with a symptomatic COVID-19 infection history showed evidence of amplified delay discounting (coefficient = 0.4554, SE = 0.2208, t = 2.0629, p = .041). In Study 2, baseline symptomatic COVID-19 history was associated with self-reported cognitive dysfunction and a latent variable reflecting psychiatric symptoms of anxiety, depression and agitation at follow-up. Mediational analyses revealed evidence of cognitive mediation of clinically significant psychiatric outcomes: depression (indirect effect = 0.077, SE = 0.026, p = .003) and generalized anxiety (indirect effect = 0.060, SE = 0.021, p = .004). ConclusionsConverging findings from laboratory and population survey data support the conclusion that symptomatic COVID-19 infection is associated with task-related, functional imaging and self-reported indices of cognitive dysfunction as well as psychiatric symptoms. In some cases, these findings appear to be more amplified among women than men, and among older women than younger.

Bestandsaufnahme der verfügbaren und aktuell eingesetzten Typisierungsmethoden einschließlich genombasierter Verfahren von Zoonoseerregern am Beispiel von Salmonella enterica

ZusammenfassungHintergrundIn den vergangenen Jahren hat sich die Gesamtgenomsequenzierung („whole genome sequencing“; WGS) in Kombination mit bioinformatischen Analysen zum Stand der Technik bei der Bewertung des Pathogenitäts- und Resistenzpotenzials sowie der Verwandtschaftsgrade zwischen Bakterien entwickelt. Die WGS-Analyse stellt somit ein zentrales Instrument bei der Typisierung von Erregern und der Untersuchung von Krankheits- und Ausbruchsclustern im Rahmen der molekularen Epidemiologie dar. Ziel der Studie war die Generierung eines Überblicks der in Deutschland auf Landes- und Bundesebene verfügbaren Erregertypisiermethoden von Salmonellen und Shiga-Toxin-bildenden bzw. enterohämorrhagischen Escherichia coli (STEC/EHEC) und den angewandten geno- und phänotypischen Methoden sowie über die Verfügbarkeit der genombasierten Typisierung und entsprechenden Analyseverfahren.MethodenIm Zeitraum vom Februar bis Juni 2020 wurde eine elektronische Umfrage bei Laboratorien durchgeführt, die für den öffentlichen Gesundheitsschutz und gesundheitlichen Verbraucherschutz tätig sind.Ergebnisse und FazitDie Ergebnisse der Umfrage zeigten, dass viele der teilnehmenden Laboratorien über eine große Auswahl an phänotypischen und molekularbiologischen Methoden verfügen. Molekularbiologische Typisierungen werden am häufigsten für die Speziesidentifizierung von Salmonellen herangezogen. WGS-Verfahren sind vielfach schon bei Einrichtungen auf Bundes- und Landesebene etabliert oder befinden sich im Aufbau. Die Illumina-Sequenzierung ist dabei die am weitesten verbreitete Technologie. Die Umfrage bestätigt die Bedeutung von molekularbiologischen und genombasierten Typisierungstechnologien für die Laboratorien bei der Diagnostik von bakteriellen zoonotischen Erregern.

Intergroup relationships with people who use drugs: A personal network approach

Positive and meaningful intergroup contact between people who use drugs and those with the potential to provide positive social interactions has been identified as an important pathway to address the burden of drug use by reducing stigmatizing views and behaviors. Traditional approaches to intergroup contact typically rely on laboratory experiments or survey vignettes to examine the consequences of variation in contact conditions and relationships. Although seldom measured, contact occurs naturally through individuals' personal social networks. Here, we apply this latter approach to examine how the characteristics of drug use and social roles are associated with positive and meaningful intergroup contact in daily life. We leverage unique data from a state representative sample of Indiana residents aged 18 or older (n = 926) that completed a personal network interview and separately reported people they know who have a drug use problem. We first identified the respondents who nominated a person who uses drugs as a member of their core personal network and then evaluated the relationship, disease, and individual characteristics that were associated with that person's inclusion in the personal network. We find that primary relationships (e.g., having a spouse or child who uses drugs) are associated with meaningful contact with people who use drugs but that intense manifestations of disease characteristics (severe or problematic, danger to self) can limit the likelihood of contact. These findings demonstrate how the nature of intergroup contact can shape the types of relationships that have been shown to help reduce stigmatizing attitudes and the behavioral barriers to recovery, such as social isolation. Thus, core networks present a valuable approach to defining the factors that likely contribute to effective intergroup contact.

2061. High Rates of Viremic HIV, Risky Sexual Behaviors, and Interest in Gender Affirming Hormone Therapy among Transgender Individuals Who Engage in Transactional Sex

Abstract Background In the U.S., HIV transmission persists largely through sexual transmission in gender and sexual minorities. Transactional sex (TS) is a known risk factor for HIV transmission, yet risk behaviors and engagement in HIV treatment and other medical services among transgender (TG) individuals who have TS are poorly understood. Methods PATCH is a natural history study of TG individuals in Washington, DC. Participants complete laboratory testing and surveys, including assessment of TS – defined as previous year sex in exchange for drugs, money or shelter. Fisher’s exact test was used for statistical analysis. Results Of 54 participants assigned male at birth (AMAB), 21(39%) endorsed TS, the majority of whom were female (81%), Black (95%), median age 35, and HIV+ (76%; Table 1). Of those with HIV, 12 (75%) were prescribed ART, though only 8 (50%) had HIV VL < 200. The majority of TG people with TS had sex weekly or more (75%), with > 5 partners/year (62%) and used their penis (80%) and anus (90%) during TS. A minority consistently used condoms in receptive anal sex (29%), penetrative anal sex (25%), penetrative vaginal sex (17%), and chem sex (8%). While 95% had ever taken gender affirming hormone therapy (GAHT), 62% were not prescribed GAHT at screening, of whom, 54% were interested in seeing a provider for GAHT. When compared to non-TS participants, TG individuals with TS were more likely to have chem sex (p< 0.01), use drugs daily or more (p=0.04), use amphetamines (p< 0.01) and cocaine (p< 0.01), and less likely to have GAHT prescribed by a provider (p=0.03). HIV+ patients with TS were less likely to have HIV VL< 200 (p=0.02). Table 1:Participant Characteristics and Associations with Transactional Sex Conclusion This cohort of AMAB TG individuals with TS had high rates of viremic HIV, and multiple risk factors for HIV transmission, including frequent condomless sex with multiple partners. Additionally, those with TS were more likely to have high risk drug use, indicating that novel strategies to decrease risk associated with chem sex, particularly stimulant use, should be prioritized. Further, nearly all TG participants with TS had taken GAHT, yet were less likely to get GAHT from a provider. As many had interest in seeing a provider for GAHT, co-locating GAHT with HIV treatment and prevention services could help providers engage this population and address HIV-related risks. Disclosures Shyam Kottilil, MD, PhD, Arbutus Pharmaceuticals: Grant/Research Support|Gilead: Grant/Research Support|Merck: Grant/Research Support|Regeneron Pharmaceuticals: Advisor/Consultant|Silverback Therapeutics: Advisor/Consultant|The Liver Company: Advisor/Consultant|Yufan Biotechnologies: Advisor/Consultant Sarah Kattakuzhy, MD, Gilead: Grant/Research Support Elana S. Rosenthal, MD, Gilead: Grant/Research Support|Merck: Grant/Research Support.

Characteristics of Peat with Different Depths in Supporting Growth and Productivity of Oil Palm

The potential for oil palm production on peatlands is very diverse; different types of peat, maturity, and depth significantly influence yields. This study aims to determine the characteristics of peat with different depths in supporting the growth and productivity of oil palm. The research was conducted at the PT Kaswari Unggul Palm Oil Plantation in Jambi Province using laboratory surveys and test methods. Observations done were Plant height, Number of Fresh Fruit Bunches (FFB), Stem Circumference, FFB Weight, Midrib Length, Palm Oil Productivity, Light Intensity. Soil analysis were available-P, total-Fe-, total N, organic-C and CEC, pH, Al-exchange, total-K, Soil Moisture, and Peat Maturity. In shallow peat, the soil is more completely decomposed and provides nutrients that can be absorbed by plants so that plants grow better. The results showed that the shallow peat has a maturity level of sapric peat (very ripe) and peat in hemic maturity (slightly mature). Also, the depth of peat will affect the value of total-N, pH, available-P, and K-exchange and does not affect organic-C, total-Fe, CEC, and Al-exchange. Moreover, the growth and production of oil palm on shallow peat were better than on deep peat.

Positive predictive values and outcomes for uninformative cell-free DNA tests: An Italian multicentric Cytogenetic and cytogenomic Audit of diagnOstic testing (ICARO study).

To establish the positive predictive values (PPV) of cfDNA testing based on data from a nationwide survey of independent clinical cytogenetics laboratories. Prenatal diagnostic test results obtained by Italian laboratories between 2013 and March 2020 were compiled for women with positive non-invasive prenatal tests (NIPT), without an NIPT result, and cases where there was sex discordancy between the NIPT and ultrasound. PPV and other summary data were reviewed. Diagnostic test results were collected for 1327 women with a positive NIPT. The highest PPVs were for Trisomy (T) 21 (624/671, 93%) and XYY (26/27, 96.3%), while rare autosomal trisomies (9/47, 19.1%) and recurrent microdeletions (8/55, 14.5%) had the lowest PPVs. PPVs for T21, T18, and T13 were significantly higher when diagnostic confirmation was carried out on chorionic villi (97.5%) compared to amniotic fluid (89.5%) (p<0.001). In 19/139 (13.9%), of no result cases, a cytogenetic abnormality was detected. Follow-up genetic testing provided explanations for 3/6 cases with a fetal sex discordancy between NIPT and ultrasound. NIPT PPVs differ across the conditions screened and the tissues studied in diagnostic testing. This variability, issues associated with fetal sex discordancy, and no results, illustrate the importance of pre- and post-test counselling.

Facile methods for reusing laboratory plastic in developmental biology experiments

Plastic pollution negatively affects ecosystems and human health globally, with single-use plastic representing the majority of marine litter in some areas. Life science laboratories prefer pristine conditions for experimental reliability and therefore make use of factory standardized single-use plastic products. This contributes to overall plastic waste in the United States and globally. Here, we investigate the potential of reusing plastic culture dishes and subsequently propose methods to mitigate single-use plastic waste in developmental biology research laboratories. We tested the efficacy of bleach and ethyl alcohol in sterilizing used dishes. We then tested the feasibility of washing and reusing plastic to culture Xenopus laevis embryos subjected to various manipulations. Cleaning and reusing laboratory plastic did not affect the development or survival of X. laevis, indicating that these cleaning methods do not adversely affect experimental outcome and can be used to sterilize plastic before reuse or recycling. Lastly, we performed a survey of various life science laboratories to estimate both waste reduction and savings associated with recycling single-use plastics. Standardization of these procedures would allow research laboratories to benefit economically while practicing environmentally conscious consumption.

The Abundance and Diversity of Plankton in the Waters of the Tasilaha Lagoon Helpano Village, South Banawa, Donggala Regency

Plankton are organisms (animals and plants) that live floating or floating in the water column, their movement is influenced by currents. The role of plankton is very important in aquatic ecosystems, especially phytoplankton which are primary producers. This research is a quantitative descriptive study using purposive sampling method with a field and laboratory survey approach. The purpose of the study was to describe the abundance and diversity of plankton in the waters of the Tasilaha Lagoon. This research was conducted in June – July 2022. The results of the study were 31 types of plankton consisting of 17 types of phytoplankton and 14 types of zooplankton. The abundance of phytoplankton species obtained ranged from 366–5,597 cells/liter and zooplankton 623–5,515 individuals/liter. The value of the phytoplankton diversity index was 2.44–2.55 and the zooplankton ranged from 2.19–2.31.

Industrial wastewater as a source of external organic carbon for the biological nutrient removal.

Addition of external organic carbon source for denitrification is generally used in wastewater treatment plants (WWTPs) to intensify nitrogen removal processes. The aim of the laboratory survey was to measure the composition of concentrated industrial wastewater, determine the possibilities of its use as an external denitrification substrate, and assess its overall impact on WWTP. The obtained results demonstrate that the analysed industrial wastewater is biodegradable, and can be used as a denitrification substrate without special adaptation of biomass. The denitrification rates with tested wastewater were in the range of 1.6 to 1.9mgN/g·h. Negative influence of long-term dosing of industrial wastewater on activated sludge were not confirmed. The effect of imported wastewater on WWTP must be assessed comprehensively, including the impact of heavy metals from wastewater on the sludge quality. The instructions on how to calculate this balance are provided in the article.

Drugged driving investigation workflow and oral fluid testing used around the world: Survey results of SOFT/AAFS Drugs and Driving and Oral Fluid Committees

Survey the international forensic toxicology community to assess approaches to drugged driving investigations and testing, increase awareness of drugs frequently detected among drivers, and promote future partnerships between TIAFT, the Society of Forensic Toxicologists (SOFT), and the American Academy of Forensic Sciences (AAFS) members. In the United States, alcohol, cannabinoids, and opioids were found in 25%, 33%, and 15% of seriously injured or killed drivers, respectively, during the second quarter of 2020. Oral fluid drug testing has gained traction in the US, but is not currently used to the same extent as in parts of the world such as Europe and in countries such as Australia. The SOFT/AAFS Drugs and Driving (DD) and Oral Fluid (OF) Committees are focused on provided professional support and development in the area of drugs and driving impairment and oral fluid drug testing for forensic toxicologists and collaborating organizations. We introduced the mission of our Committees at the virtual 58th Annual meeting of TIAFT. We hope the survey results included herein will further future joint projects with TIAFT. A survey was developed by the SOFT/AAFS DD and OF Committees to collect information on drugged driving investigation and analytical workflows, laws and policies, commonly tested specimen types, and the most prevalent drugs detected in DUID testing. The survey consisted of 20 questions distributed using SurveyMonkey to over 100 TIAFT representatives from different countries and jurisdictions. Participant responses were reviewed and summarized. Specific comprehensive programs with selected for detailed follow-up and featured in spotlight workflows to provide guidance and models to less developed programs. Cannabinoids and ethanol were the most commonly detected drugs in driving cases with methamphetamine/amphetamine, MDMA, fentanyl, 6-MAM/morphine, and cocaine frequently encompassing the top five most prevalent drugs. Blood was the specimen of choice for DUID testing and traffic fatalities followed by oral fluid and urine. Oral fluid confirmation devices included Quantisal® and Oral-Eze®. Roadside oral fluid devices included Draeger DT5000®, Alere SoToxa®, and Drugwipe®. The most common per se limit for ethanol was 0.05%. The practice of stop testing limits, where drugs are not analyzed if a blood alcohol concentration reaches a specific concentration (e.g., 0.10%), is less common outside the US. SOFT/AAFS DD and OF Committees are actively engaged in various projects to support its mission including offering special sessions at the SOFT and AAFS annual meetings, sponsoring drugs and driving and oral fluid drug testing related workshops, providing literature lists on the SOFT website, and conducting surveys of laboratories to gather information and facilitate advancement of the drugged driving investigations and testing. Our goal is to collaborate with TIAFT membership in sharing drug trends and laboratory practice while supporting prevention efforts to reduce drug-impaired driving. The survey results highlight differences and similarities among countries in their drugged driving investigation processes. This project will encourage future partnerships and continuing education opportunities.

Plant Water Use Efficiency for a Sustainable Agricultural Development

The rising shortage of water resources worldwide in crop-producing regions and the need for irrigation optimisation call for sustainable water savings. That is, the allocation of irrigation water will be an ever-increasing source of pressure because of vast agricultural demands under changing climatic conditions. Consequently, irrigation has to be closely linked with water-use efficiency with the aim of boosting productivity and improving food quality, singularly in those regions where problems of water shortages or collection and delivery are widespread. The present Special Issue (SI) contains 19 original contributions addressing water-use efficiency under challenging topic of sustainable irrigation management to meet water scarcity conditions. These papers cover a wide range of subjects, including (i) interaction mineral nutrition and irrigation in horticultural crops, (ii) sustainable irrigation in woody fruit crops, (iii) medicinal plants, (iv) industrial crops, and (v) others devoted to remote sensing techniques and crop water requirements, genotypes for drought tolerance, and agricultural management platform. The studies have been carried out in both field and laboratory surveys, as well as modelling studies, and a wide range of geographic regions are also covered. The collection of these manuscripts presented in this SI updates and provides a relevant knowledge contribution for efficient saving water resources.

Validation of a commercial line blot for the detection of serum anti-Ro60 autoantibodies

Serum anti-Ro60 is one the most frequently encountered autoantibodies in the diagnostic immunopathology laboratory and in clinical practice. A large variety of assays are available to detect this including the popular multiplex line immunoblot (IB) assay. We evaluated the analytical performance of the IB for anti-Ro60 detection, using the counterimmunoelectrophoresis (CIEP) method as the 'gold standard'. We also undertook a survey of international laboratories, who use the IB, about their reporting practices for anti-Ro60. Using the manufacturer's reported cut-off of 15 units, the IB has an analytical sensitivity of 90.9% and specificity of 99.3% for anti-Ro60 detection. The optimal cut-off to balance sensitivity and specificity was determined to be 5 units with a sensitivity of 100% and specificity of 97.4%. Most laboratories use the manufacturer's specified cut-off (15 units) when determining a positive anti-Ro60 result. Whilst the commercial IB generally performs well, laboratorians need to be mindful of the limitations of IB in detecting antibodies that recognise conformational epitopes and what cut-offs they use. A vast majority of laboratories could potentially miss detection of this clinically important autoantibody.

LoVoCCS. I. Survey Introduction, Data Processing Pipeline, and Early Science Results

We present the Local Volume Complete Cluster Survey (LoVoCCS; we pronounce it as “low-vox” or “law-vox,” with stress on the second syllable), an NSF’s National Optical-Infrared Astronomy Research Laboratory survey program that uses the Dark Energy Camera to map the dark matter distribution and galaxy population in 107 nearby (0.03 < z < 0.12) X-ray luminous ([0.1–2.4 keV] L X500 > 1044 erg s−1) galaxy clusters that are not obscured by the Milky Way. The survey will reach Vera C. Rubin Observatory Legacy Survey of Space and Time (LSST) Year 1–2 depth (for galaxies r = 24.5, i = 24.0, signal-to-noise ratio (S/N) > 20; u = 24.7, g = 25.3, z = 23.8, S/N > 10) and conclude in ∼2023 (coincident with the beginning of LSST science operations), and will serve as a zeroth-year template for LSST transient studies. We process the data using the LSST Science Pipelines that include state-of-the-art algorithms and analyze the results using our own pipelines, and therefore the catalogs and analysis tools will be compatible with the LSST. We demonstrate the use and performance of our pipeline using three X-ray luminous and observation-time complete LoVoCCS clusters: A3911, A3921, and A85. A3911 and A3921 have not been well studied previously by weak lensing, and we obtain similar lensing analysis results for A85 to previous studies. (We mainly use A3911 to show our pipeline and give more examples in the Appendix.)

MO190: Experiences of Being a Living Kidney-Donor-Perceived and Measured Stress Levels before and after Kidney Donation

Abstract BACKGROUND AND AIMS Living kidney donation is the preferred kidney replacement therapy considering graft function and survival rates. To donate a kidney is a life-changing experience, and there is a diverse spectrum of emotions connected to this process. The aim of this study was to investigate kidney donors' perceived and measured stress levels before and after donation. Furthermore, to investigate if the level of perceived stress could be linked to the degree of metabolic stress measured using saliva cortisol, serum glucose and insulin levels. METHOD A prospective observational study including 83 patients (46 ± 10.9 years, 63% women), who donated a kidney at Sahlgrenska University Hospital, Gothenburg, during 2009–2010. Three self-rating surveys concerning stress (Everyday Life Stress Scale), vital exhaustion (Maastricht Questionnaire of vital exhaustion) and depressive symptoms (Depressive Mood Scale) were answered on the day before surgery, and then 2 and 6 months after kidney donation (see score details, Table 1). Blood samples of insulin, glucose and saliva cortisol were collected at the same time points. Statistical analyses were carried out to investigate changes over time, potential gender differences, and correlations between laboratory data and survey results. RESULTS Kidney donors generally scored low in self-rating stress mean 15.5 and depression symptom scales mean 10.8 pre-donation. The Maastricht's questionnaire showed a trend toward significant gender difference at 2 months; women mean score was 32.1 ± 9.3 and men 35.8 ± 6.9, P = .092. At 6 months, there was a significant gender difference in self-reported vital exhaustion, where women scored higher 36.9 ± 7.1 than men 30.7 ± 11.6; P = .037 and a near significant difference on the Depressive Mood Scale, 12.2 ± 12.5 versus 6.4 ± 8.2 at 6 months; P = .058. No significant difference over time in experienced well-being (Everyday Life Stress Scale) was found, 15.6 ± 8.2 vs 14.3 ± 7.6, P = .554. Data are displayed in detail in Table 1 and Figure 1. No correlations between metabolic stress measured as salivary cortisol (morning, noon and evening) and HOMA-IR, and perceived stress were found. CONCLUSION Kidney donors self-reported stress level and depressive symptoms did not change pre-donation to 6 months after donation. However, women reported more feelings of exhaustion and stress than men did at 6 months post-donation. In the future, a more structured psychosocial follow-up of kidney donors after at least 6 months is suggested.

A Survey of Human Gross Anatomy Laboratories in DPT Programs across the United States

Purpose: The purposes of this study were to 1) describe the current teaching methodology used in Doctor of Physical Therapy (DPT) human gross anatomy (HGA) labs, 2) examine the demographics and perceptions of HGA instructors and compare responses based on years of experience, 3) determine the utilization and instructor perceptions related to cadaver dissection and other methods of instruction, and 4) determine which safety/security protocols are used in HGA laboratories. Method: All DPT programs (N=250) in the United States (US) accredited by the Commission on Accreditation of Physical Therapy Education (CAPTE) were eligible to participate. The anonymous, 89-item online survey was completed by either an HGA instructor or DPT program director in March of 2020. Seventy-four individuals responded for a response rate of 29.6%. Results: Respondents represented programs from 65.2% private and 34.8% public institutions. Fifty percent of respondents dedicated 31-60% of their HGA course to face-to-face lab time, with 68% reporting an instructor to student ratio in lab of 1:15 or smaller. Seventy percent of instructors were US licensed PTs, and 78% of those PTs held an academic doctorate. The average years of anatomy teaching experience was 11.3. Ninety-six percent of programs utilized cadavers. Most programs (86%) had students perform hands-on cadaver dissection. Overall, 90% of instructors incorporated learning activities into lab beyond dissection. Ninety-four percent of instructors reported enjoyment teaching HGA, and a majority felt they had adequate teaching support and academic preparation. Sixty percent of respondents felt that cadavers were the only way to teach lab, while 90% felt that cadavers were the best way to teach lab. Regarding safety, 38% of instructors had concerns regarding chemical exposure in lab, and 11% believed their health was at risk. Comparative analyses found significant differences in instructor perceptions based on years of anatomy teaching experience (+/- 10 years). Less experienced faculty were more likely to believe that a non-cadaver approach to teaching HGA can be as effective as using cadavers given the right technology, while more experienced faculty were more likely to believe that teaching HGA with cadavers was the best way to teach lab. Conclusions: DPT program directors and instructors may find this study valuable to compare their HGA course(s) to other programs in the US. Although there is a clear preference for including cadavers in HGA laboratories, it is evident that most instructors are incorporating other learning approaches in their HGA laboratories.

Search for familial hypercholesterolemia patients in an Italian community: A real-life retrospective study

Background and aimsFamilial hypercholesterolemia (FH) is a common inherited disorder of low-density lipoprotein (LDL) catabolism that causes elevated LDL-cholesterol (LDL-C) and premature atherosclerotic cardiovascular disease (ASCVD). Despite the availability of effective treatments, FH remains underdiagnosed and undertreated. The aims of the study were to identify putative FH subjects using data from laboratory and cardiology databases, genetically characterize suspected FH patients referred to the Lipid Clinic and monitor attainment of treatment goals in identified patients. Methods and resultsWe retrieved the electronic health records of 221,644 individuals referred to laboratory for routine assessment and of 583 ASCVD patients (age ≤65) who underwent percutaneous transluminal coronary angioplasty (PTCA). We monitored the lipid profiles of subjects with LDL-C ≥ 250 mg/dl identified by laboratory survey (LS-P), PTCA patients and patients from the Lipid Clinic (LC-P). The laboratory survey identified 1.46% of subjects with LDL-C ≥ 190 mg/dl and 0.08% with LDL-C ≥ 250 mg/dl. Probable/definite FH was suspected in 3% of PTCA patients. Molecularly-confirmed FH was found in 44% of LC-P subjects. Five new LDLR mutations were identified. The 50% LDL-C reduction target was achieved by 70.6% of LC-P patients. Only 18.5% of PTCA patients reached the LDL-C < 55 mg/dl target. ConclusionBy using a combined approach based on laboratory lipid profiles, documented ASCVD and Lipid Clinic data, we were able to identify subjects with a high probability of being FH. Attainment of LDL-C goals was largely suboptimal. Efforts are needed to improve FH detection and achievement of lipid targets.