Laboratory Scale Research Articles

Disintegration of commercial single-use plastics from synthetic and biobased origins and effects on plant growth

Many single–use plastic (SUP) options made of synthetic polymers, bio-based materials, and blends of both are available in the market and used in large quantities. The disintegration of eleven commercial SUP, marketed in Mexico as cups and plates, was investigated in an aerobic home compost environment at a laboratory scale over 180 days. An evaluation of chemical changes, surface morphology, and thermal and mechanical properties was conducted to ascertain the original composition of SUP and the progression of disintegration in samples that are challenging to clean from soil contamination. Furthermore, the impact of residual compost on barley (Hordeum vulgare) plant growth and its correlation with the leaching of heavy metals were explored. The bio-based SUP, but not those made of expanded polystyrene foam, showed a correlation between the disintegration degree (measured by weight loss into particles <2 mm) and a decrease in functional groups (observed by FT-IR), mechanical-thermal stability loss, and surface wear over disintegration time. For instance, the highest disintegration at 180 days was approximately 70% for wheat bran and palm leaf plates, followed by wheat plates and cellulose-PLA cups (60%). In addition to the components listed by the manufacturers, the FT-IR and DSC analysis revealed the presence of polyethylene and polypropylene in cellulose cups and sugarcane plates. These components, impede disintegration but contribute to preserving thermal resistance and hydrophobicity during utilization. Compost derived from expanded polystyrene foam SUP, with 90 days of disintegration, was rich in zinc and chromium and significantly decrease in the root length of the barley plant compared to the control. This demonstrates the necessity of considering the impact of the leaching of additives and secondary microplastics into the environment.

Optimizing Bleaching Process Parameters of Distillers Corn Oil for edible applications using a response surface methodology

The bleaching process is aimed at removing pigments, traces of metals, and residual phospholipids to improve the color, stability, and quality of the oil. Distillers corn oil (DCO) contains lots of β-carotene that are beneficial but no study has focused on optimizing the removal of pigments while minimizing the loss of β-carotene. Hence, a response surface methodology with the Box-Behnken design was used to study the effect of the process parameters during bleaching. The process parameters were dosage of bleaching clay (1 − 3%), time (5 − 25 min), and temperature (90 − 110 °C) for neutralized DCO on the laboratory scale. The optimal parameters were bleaching clay dosage of 1.2%, time of 23.2 min, and temperature of 99.1 °C. The β-carotene reduced from 0.2% to 36%, red color reduced from 27% to 88%, and yellow color reduced from 0% to 83%. The PV values of the bleached oil sample ranged from 1.7 to 9.4 meq O2/kg. The quality of DCO at the optimal bleaching level was compared with conventional edible corn oil. The models developed was used to identify the precise balance between enhancing color removal and maintaining beneficial nutritional values.

Examination of the Dry Density Importances in TDR Use and of the ASTM D6780 Standard Based on a New Calibration Procedure

Abstract The use of time domain reflectometry (TDR) to monitor soil water content at either field or laboratory scales is highly common. Regarding the soil dry density, ρd, effect on the TDR application, three main approaches are found in the literature: (1) Ignore the density issue and use calibration to evaluate the water content regardless of the density. This approach is basic, yet common. (2) Include soil density in the calibration equation and use calibration for the water content as a function of both the TDR output and soil density. In this approach, the soil density should be provided from an independent source. (3) Determined ρd from the TDR waveform. In this case both water content and ρd are set from the TDR analysis. In cases where the ρd is not available, in order to use TDR for water content measurement, a decision has to be made whether to address the density issue although it requires resources, or to neglect it. This article reviews this issue and validates the density effect using an efficient TDR calibration methodology. A calibration scheme that controls ρd of the specimen has been developed and applied. The methodology creates continuous calibration curves over a range of volumetric water content from a single test specimen. Based on the results, it has been demonstrated that the soil density has a major effect on the TDR output and it cannot be ignored. In addition, this article presents a unique examination of ASTM D6780, Standard Test Methods for Water Content and Density of Soil In Situ by Time Domain Reflectometry (TDR). The ASTM dry density estimation was found to provide reliable results; however, the gravimetric water contents estimation gives unsatisfactory results, especially for low densities.

Pyrolysis of industrial hemp biomass from contaminated soil phytoremediation: kinetics, modelling transport phenomena and biochar-based metal reduction

Phytoremediation is the use of vegetation for the in situ treatment of contaminated environments. After plants have been used for phytoremediation of soils, their biomass can be used for example as value-added products or converted by thermochemical processes. Large-scale application of pyrolysis technology for phytoremediation biomass requires accurate predictive kinetic models and a characterization of the toxicity of the materials produced. The pyrolysis of industrial hemp (Cannabis sativa L.) was investigated on a laboratory scale by varying the process conditions and accurately modelled by considering four pseudo-components with first reaction order. The average value of the coefficients of determination is 0.9980. Biomass and biochar were characterized and the main components of the gas phase were monitored. We found Cd, Pb, and Zn in the roots, although in lower amounts than in the soil. Especially the leaves and stems showed negligible traces of these elements, so that these parts can be used directly, even if the hemp was grown on the polluted soil. After pyrolysis, the concentration of pollutants in the solid fraction decreased, which could be attributed to the reduction of metal oxides (or salts) to elemental form and subsequent evaporation. This pyrolysis process has the potential to treat heavy metal-rich biomass, with gas phase purification via condensation, yielding agricultural-grade biochar, CO-rich gas and a highly concentrated heavy metal stream in absorbent material.

Protective effects of lemon nanovesicles: evidence of the Nrf2/HO-1 pathway contribution from in vitro hepatocytes and in vivo high-fat diet-fed rats

The cross-talk between plant-derived nanovesicles (PDNVs) and mammalian cells has been explored by several investigations, underlining the capability of these natural nanovesicles to regulate several molecular pathways. Additionally, PDNVs possess biological proprieties that make them applicable against pathological conditions, such as hepatic diseases. In this study we explored the antioxidant properties of lemon-derived nanovesicles, isolated at laboratory (LNVs) and industrial scale (iLNVs) in human healthy hepatocytes (THLE-2) and in metabolic syndrome induced by a high-fat diet (HFD) in the rat. Our findings demonstrate that in THLE-2 cells, LNVs and iLNVs decrease ROS production and upregulate the expression of antioxidant mediators, Nrf2 and HO-1. Furthermore, the in vivo assessment reveals that the oral administration of iLNVs improves glucose tolerance and lipid dysmetabolism, ameliorates biometric parameters and systemic redox homeostasis, and upregulates Nrf2/HO-1 signaling in HFD rat liver. Consequently, we believe LNVs/iLNVs might be a promising approach for managing hepatic and dysmetabolic disorders.

Relationship between rheological parameters and structure formation in high moisture extrusion of plant protein biopolymers

High moisture extrusion (HME) is widely employed to texturize plant-derived protein ingredients, but its development is still much based on trial and error. In this project, it was hypothesized that the rheological properties of plant-based mixtures analyzed when in the molten state and during their cooling can aid in understanding their structure formation. To test this hypothesis, biopolymer formulations containing pea protein isolate (PPI) were examined at different moisture and starch contents, and their rheological properties were analyzed using a closed cavity rheometer (CCR) at temperatures relevant to those applied during HME processing. The results obtained with the CCR were then contrasted with the mechanical properties of HME obtained using a lab scale extruder, measured using oscillatory rheology, large deformation, and dynamic mechanical analysis. Low moisture (55 %) HMEs were stiff and brittle, while high moisture (65 %) HMEs formed more flexible and anisotropic structures. Addition of starch created softer structures. The viscoelastic properties of the biopolymer mixes measured with the CCR were correlated with the mechanical parameters of the final extrudates. Strong correlations were found between small deformation rheological parameters measured in the CCR and the hardness values, while non-linear viscoelastic parameters were correlated with anisotropy indexes. Results demonstrated that the material properties measured at the early cooling stages strongly influence the structural heterogeneity in HMEs. This study highlights the potential to use the viscoelastic properties of the biopolymer mix measured with the CCR to predict their structural features when processed by extrusion.

Investigation of the flash vaporization technique as a novel anolyte separation method in a copper-chlorine thermochemical cycle for sustainable hydrogen production

Thermochemical water-splitting processes are a promising alternative to the conventional hydrogen production technologies. In this regard, the thermochemical copper-chlorine cycle is amongst the clean hydrogen production processes that have shown immense promise to achieve commercialization in the long term future through several investigations reported in the open literature. A primary reason for this is its moderate temperature requirements which can be harnessed through a wide variety of sources such as waste heat or solar thermal energy. More specifically, the four-step variant of the copper-chlorine cycle has exhibited the highest efficiencies compared to its three and five-step versions. However, certain aspects of the cycle need further attention. One such aspect is the fourth and the final step of the cycle – the anolyte separation step which ensures the complete replenishment of all chemical compounds within the cycle. The anolyte is a homogeneous solution that constitutes water, hydrochloric acid, copper (II) chloride, and copper (I) chloride. The existing methodology for the separation of all these chemicals considers a multi-step drying process that takes place at ambient pressure and temperatures over 100°C. These conditions have been concluded to contribute to lowering the energy efficiency and increasing the cost rate of hydrogen through various studies previously conducted and published by the authors. In this regard, the authors have investigated an alternative technique for the anolyte separation process – the flash vaporization method where the partial separation of various chemical species can be achieved under vacuum conditions resulting in lower required temperatures and ultimately making the anolyte separation process relatively less energy intensive. Thus, this paper develops a stand-alone experimental set-up to investigate the flash vaporization process on a lab scale to determine its feasibility and potential to be a replacement for the existing approach of anolyte separation in a four-step copper-chlorine thermochemical cycle. The experiments are conducted under a wide variety of conditions and the obtained results are quite promising. Further, this study also considers the development of an empirical model to enable an analytical quantitative prediction of the separated anolyte solution.

Non-equilibrium turbulent boundary layers in high reynolds number flow at incompressible conditions: effects of streamline curvature and three dimensionality

The physics and computational prediction of turbulent boundary layer flow over axisymmetric and three-dimensional bodies are examined. Three cases were considered for which extensive experimental results and companion Reynolds-averaged Navier Stokes (RANS) solutions were obtained and/or available in the open literature. These cases all have Reynolds numbers based upon the freestream velocity and body geometric scale on the order of 10 5 to 10 6 , which is large for laboratory scales but small compared to the maximum scales observed for full-scale vehicles. Despite significant differences in approach flow fields and geometries for these three cases, some common themes emerged in the findings. All cases involved complications due to pressure gradients combined with streamwise curvature, and all exhibited regions of turbulence reduction due to accelerated flow. These complications led to discrepancies in computed results even in attached flow regions where it is often assumed that RANS models provide reliable predictions. The authors recommend further work on modelling approaches that can capture rapid distortion effects on turbulence transport that can be incorporated into industry-useful frameworks. Two cases involving laterally symmetric, three-dimensional wall-mounted hills with aft-body separation revealed that asymmetric mean flow fields are likely to result. This finding has been observed in experiments conducted in multiple facilities and in computations using multiple solvers and turbulence models. It is concluded that non-unique and asymmetric global flow solutions are fundamental to flow cases with lateral geometric symmetry involving turbulent boundary layer separation. Further work is also needed to accurately predict low-frequency unsteadiness due to geometries that produce non-unique mean flow fields. For such flows, it remains to be definitively determined whether experimentally observed modes of the mean flow are equivalent, or nearly equivalent, to asymmetric mean flow solutions obtained using RANS approaches.

Seismic Response of Hectometer‐Scale Fracture Systems to Hydraulic Stimulation in the Bedretto Underground Laboratory, Switzerland

AbstractWe performed a series of hydraulic stimulations at 1.1 km depth in the Bedretto underground laboratory, Switzerland, as part of an overall research strategy attempting to understand induced seismicity on different scales. Using an ultra‐high frequency seismic network we detect seismic events as small as Mw &lt; −4, revealing intricate details of a complex fracture network extending over 100 m from the injection sites. Here, we outline the experimental approach and present seismic catalogs as well as a comparative analysis of event number per injection, magnitudes, b‐values, seismogenic index and reactivation pressures. In our first‐order seismicity analysis, we could make the following observations: The rock volume impacted by the stimulations in different intervals differs significantly with a lateral extent from a few meters to more than 150 m. In most intervals multiple fractures were reactivated. The seismicity typically propagates upwards toward shallower depth on parallel oriented planes that are consistent with the stress field and seem to a large extent associated with preexisting open fractures. This experiment confirms the diversity in seismic behavior independent from the injection protocol. The overall seismicity patterns demonstrate that multi‐stage stimulations using zonal isolation allow developing an extended fracture network in a 3D rock volume, which is necessary for enhanced geothermal systems. Our stimulations covering two orders of magnitude in terms of injected volume will give insights into upscaling of induced seismicity from underground laboratory scale to field scale.

Synergistic effects of organic matter degrading and electroactive biofilms for rapid startup of sediment microbial fuel cells: From laboratory scale to in situ application

Synergistic effects of organic matter degrading and electroactive biofilms for rapid startup of sediment microbial fuel cells: From laboratory scale to in situ application

Small scale laboratory monotonic and cyclic pull out testing on grout and resin encapsulated cable bolts

Axial studies on cable bolts can be conducted using various scale testing apparatuses. Large scale testing, while providing a powerful platform for testing, is expensive and time consuming. This study presents details of a small scale pull out testing campaign on cable bolts and investigates the results achieved. Six popular types of cable bolts were studied using an anti rotation apparatus while encapsulated in cementitious grout and resin. The resin samples were tested under both monotonic and cyclic loading patterns. The results showed that grouted bulbed cables require higher displacement to reach their maximum load capacity which is lost at failure, while plain cables tend to hold lower loads for a longer time. Resin samples provided strain softening behaviour with low capacities, particularly in absence of cable indentation or bulbs. Cyclic loading tended to adversely affect the post peak behaviour of the resin samples, especially in the bulbed cables. Failed samples inspected after the testing suggested a non-uniform damage profile along the cable with extensive damage at the exit point transitioning into almost no damage at the entry point.

Silica-Based Composite Sorbents for Heavy Metal Ions Removal from Aqueous Solutions.

Weak polyelectrolyte chains are versatile polymeric materials due to the large number of functional groups that can be used in different environmental applications. Herein, one weak polycation (polyethyleneimine, PEI) and two polyanions (poly(acrylic acid), PAA, and poly(sodium methacrylate), PMAA) were directly deposited through precipitation of an inter-polyelectrolyte coacervate onto the silica surface (IS), followed by glutaraldehyde (GA) crosslinking and extraction of polyanions chains. Four core-shell composites based on silica were synthesized and tested for adsorption of lead (Pb2+) and nickel (Ni2+) as model pollutants in batch sorption experiments on the laboratory scale. The sorbed/desorbed amounts depended on the crosslinking degree of the composite shell, as well as on the type of anionic polyelectrolyte. After multiple loading/release cycles of the heavy metal ions, the maximum sorption capacities were situated between 5-10 mg Pb2+/g composite and 1-6 mg Ni2+/g composite. The strong crosslinked composites (r = 1.0) exhibited higher amounts of heavy metal ions (Me2+) sorbed than the less crosslinked ones, with less PEI on the surface but with more flexible chains being more efficient than more PEI with less flexible chains. Core-shell composites based on silica and weak polyelectrolytes could act as sorbent materials, which may be used in water or wastewater treatment.

Formulation and evaluation of herbal shampoo

The main objective of these study is to evaluate and formulate herbal shampoo. Shampoo are one of the cosmetic products used in daily life. Synthetic preservatives and detergents have sometimes been the cause of adverse effects among consumers. A more radical approach in reducing the synthetic ingredients is by incorporating natural ingredients whose functionality is comparable with their synthetic ingredients. The ingredients used in formulation of Herbal Shampoo are Ficus Religiosa leaves (Powder), Hibiscus Leaves (Powder), Reetha (Powder), Shikakai (Powder), Aloevera, then prepared by mixing with each other and evaluated for its organoleptic and physio-chemical characteristics and performance tests in terms of wetting time test, pH, solid contents, surface tension, dirt dispersion, foam Stability, and Viscosity was performed. The combination of several such ingredient of herbal origin has made it possible to secure highly effective dry powder shampoo. The formulation at laboratory scale was done and evaluated for number of parameters to ensure its safety and efficacy The Formulated Herbal Shampoo was clear and good appealing. It demonstrated good froth stability, detergency, good cleansing, small bubble size, low surface strain, and execution of good conditioning property. The physicochemical evaluation of the formulated shampoo showed ideal results. However, to improve its quality, product performance, and safety, further development was required.

Impact of Natural Phytosanitary Product Residuals on Yeast Fermentation Performance and Wine Composition.

Although phytosanitary treatments are necessary to protect grapes from parasitic diseases, consumers are increasingly concerned about the use of synthetic phytosanitary products and their possible residues in wine. Pre-harvest phytosanitary treatments are often inevitable, and consequently downtime is required to avoid possible residues on the grapes. Instead, natural phytosanitary products, such as essential oil (EO)-based products, can be applied close to the harvest without specific restrictions, with results that are not only technically convenient but also more attractive for the consumers. Because of the high antimicrobial activity of EO products, in the present study we evaluated the effect of different residual amounts of two new EO-based phytosanitary products on the alcoholic fermentation and the chemical composition of the final fermented products. In particular, two EO-based new formulations, exploitable in organic viticulture management, were evaluated. Increasing concentrations of each formulation were tested during laboratory scale fermentations and in comparison with synthetic and natural commercial phytosanitary products. Growth and fermentation kinetics of a commercial yeast strain of Saccharomyces cerevisiae and the chemical and sensory profiles of the final products were evaluated. Both new formulations showed no significant impact on the growth and fermentation kinetic of S. cerevisiae at any of the concentrations tested. In all trials, alcoholic fermentation was completed in 15 days. Instead, a different chemical composition of the final products was observed. Therefore, these new products might represent an interesting alternative tool to the conventional phytosanitary treatments, being applicable close to the harvest without negative impacts on the kinetics of alcoholic fermentation and also being more acceptable to wine consumers.

Screening of Cation Exchange Membranes for an Anthraquinone‐Ferrocyanide Flow Battery

AbstractThe disodium salt of 9,10‐anthraquinone‐2,7‐disulphonic acid (2,7‐AQDS) is an interesting platform for developing anthraquinone derivative negolytes for aqueous organic flow batteries. Recently, ammonium sulphate supporting electrolytes have been considered for improved stability and solubility. This work advances the 2,7‐AQDS/ferrocyanide flow battery with an ammonium sulphate supporting electrolyte (pH 5) by studying the suitability of six commercially available cation exchange membranes: E‐620, NR‐212, FS‐930, F‐1075‐PK, F‐1850 and N‐115. Cell cycling under galvanostatic regime plus potential hold was performed to determine coulombic efficiency, energy efficiency and accessible capacity for each membrane as well as capacity fade rate for three selected membranes under extended operation. Cell cycling under galvanostatic control only was carried out to observe transient membrane behavior alongside accessible capacity and apparent capacity fade rate. It was found that the capacity set by the limiting negolyte is consistent with 1.5 electrons per 2,7‐AQDS molecule and that energy efficiency shows a simple direct relationship to membrane thickness, with one exception. Meanwhile, four membranes displayed similar apparent capacity fade rates at this laboratory scale irrespective of their thickness, with capacity loss explained in terms of crossover. The best overall performance was attained by the thinnest membranes, E‐620 and NR‐212.

Supercritical mechano-exfoliation process

The intricate balance among cost, output, and quality has substantially hindered the practical application of graphene within the downstream industry chain. Here we present a scalable and green supercritical CO2-assisted mechano-exfoliation (SCME) process that omits the use of organic solvents and oxidants throughout the production lifecycle, including exfoliation, separation, and purification. The SCME process achieves graphene powder space-time yields exceeding 40 kg/(m³·day) at laboratory (0.06–0.2 kg) and pilot scales ( > 4 kg), with resultant free-standing films showing conductivities up to 5.26 × 10⁵ S/m. Further kinetic investigations propose general guidelines for grinding-assisted exfoliation: (1) the macroscopic optimizing ability of mechanotechnics for mass transfer frequency and stress distribution and (2) the microscopic multiplication ability of exfoliation medium for shear-delamination. The comprehensive techno-economic analysis also underscores the economic viability of the SCME process for large-scale production.

The Re-Crushing Spatiotemporal Evolution Law of Broken Coal in the Goaf for Sustainable Utilization of Abandoned Mines

The broken coal samples’ (BCS) re-crushing characteristics in the goaf during roof compaction directly affect the mechanics and seepage characteristics of the caving zone. This will further affect the safety of coal mining and the sustainable utilization of abandoned mines. Thus, the experiment of BCS compaction is carried out with the help of an acoustic emission (AE) monitoring system. The Hurst exponent changes of the AE counts at different stages were obtained using the R/S analysis method. The results indicate that the compaction and re-crushing of the BCS at the laboratory scale have long-term memory. When providing sufficient stress, the AE activity of BCS will continue to develop according to the current trend. Based on the AE breakage location technology, the spatial distribution re-crushing characteristics of the BCS are obtained. Re-crushing of the BCS demonstrates uniform breakage in the horizontal direction and layered breakage in the vertical direction. In the horizontal direction, the boundary area first began to break, and the damage gradually spread evenly to the central area. In the vertical direction, the upper layer was the first to be broken, and then the damage began to shift to the middle and lower layers.

Scalable electrospinning using a desktop, high throughput, self-contained system

Electrospinning is a specialized processing technique for the formation of submicron diameter fibers of polymeric and ceramic materials using an electrostatic field. The process has multiple advantages over other nano- and micro- fiber synthesis methods; however, generally suffers from very low fabrication speeds, making it undesirable for scalability. This work assesses the performance of a needle-less, self-contained, high throughput electrospinning system. It further compares the fiber fabrication rates obtained versus two single needle setups with different collectors: (i) a conventional single needle and flat plate geometry, and (ii) a single needle with a rotating collector geometry. Polyvinylpyrrolidone (PVP) in ethanol was used as the model material. The fabrication rate of the high throughput system “HTES” was measured at about 2.6 g/h and was about 15 times that collected of the flat plate. Comparing it to other systems reported in the literature also proved it to be a viable option for high throughput, lab scale electrospinning.

Considerations for digitalisation of nickel electroforming

ABSTRACT This paper is a ‘follow-on’ from a paper previously published in this journal dealing with the laboratory to pilot scaling up approach using Industry 4.0 manufacturing methods. In particular, the paper reports a strategy for developing a model for the electroforming of nickel from a sulphamate electrolyte at laboratory scale which could subsequently provide an educated approach for transferring the process to a larger scale. At the laboratory scale, a rotating disc electrode assembly was used, which is a standard instrument to determine electrochemical parameters. Thereafter, small scale nickel discs were electroplated using this equipment, and a model of this process was developed and validated against those experimental results. These parameters were then used to actually produce electroforms in a prototype, 18 L tank system. Cross-validation between practical experiments and simulations followed which allowed for fine-tuning the model until it was consistently predicting the real process results within an acceptable error. Overall, it was found that a secondary current distribution model could be used for reasonably accurate description for the electroforming process, and could provide a quick virtual tool at a production facility.

Laboratory-Scale Implementation of Standardized Reconstituted Geothermal Water for Electrochemical Investigations of Carbon Steel Corrosion

Currently, the demand for heat production by geothermal energy is increasingly strong amid the controversy surrounding non-renewable forms of energy. In France, the Dogger aquifer in the Paris Basin (DAPB) produces saline geothermal waters (GWs) that are hot (70–85 °C), anaerobic, and slightly acidic (pH 6.1–6.4), and are characterized mainly by the presence of Cl−, SO42−, CO2/HCO3−, and H2S/HS. These GWs are corrosive, while the well casings used are carbon steel. GWs have been continuously treated since the 1990s by corrosion inhibitors at the bottom of production wells to reduce water–steel interactions and scaling issues. Electrochemical experiments to optimize inhibitors were carried out on site, protected from the ambient atmosphere, with actual geothermal water, using water tapping at the wellhead. Currently, carbon steel corrosion/scaling, corrosion inhibition phenomenology, and kinetics evaluation remain important challenges. These issues are, of course, linked to the durability of installations. The novelty of our work consists of our validation of a modus operandi that properly reproduces, at the laboratory scale, operating conditions similar to those encountered on the types of geothermal installations. Particular attention was paid to characterizing waters and gases from 13 production wellheads that were modelled with PhreeqC® Version 3 hydrogeochemical software and the Thermoddem thermodynamic database for implementing standardized reconstituted geothermal water (SRGW), a well-balanced water representative of the major elements and dissolved gases of actual DAPB geothermal waters. The developed electrochemical setup enabled us to analyze corrosion mechanisms such as those observed on site and to investigate corrosion inhibition using petrosourced and biosourced inhibitors. The modus operandi constitutes a reference for further investigations, at the laboratory scale, of corrosion inhibition. These investigations may include screening and optimizing the formulas of petrosourced and biosourced inhibitors for use in DAPB waters.