Laboratory-scale Study Research Articles

Nitrogen recovery from anaerobic digestate via ammonia stripping and absorbing with a nitrified solution

Anaerobic digesters produce renewable energy from organic wastes, including animal wastes. However, ammonia toxicity stemming from the nitrogen content of animal wastes reduces the feasibility of the process. Approaches for stripping ammonia and recovering it via the use of strong mineral acids have disadvantages. Here, we evaluate a process that includes ammonia removal coupled with a novel nitrogen recovery strategy using a solution from biological nitrification. This approach has the potential to reduce ammonia toxicity while providing valuable biofertilizer from nitrification. Since this integrated process couples sub-systems that have different operating conditions, an experimental laboratory-scale study and Aspen Plus model of the nitrogen removal and recovery system (consisting of stripping and absorption units) were used to assess process performance as a function of stripping unit temperature and feed pH, as well as of the absorption unit feed pH and nitrogen concentrations. Based on results of the experiments and modeling, the recommended conditions for the integrated stripper-absorber system were a feed pH of 10 and operating temperature of 50 °C for the stripping unit, and a feed pH of 7 and feed nitrogen concentrations of 7 g-N/L for the absorption unit. While the feed pH and temperature had a large impact on ammonia stripping, neither the feed pH nor the nitrogen concentration in the feed led to a significant difference in the ammonia absorption. These results support the use of this new approach of using nitrified solution as the ammonia absorbent in an integrated anaerobic digestion process.

Prospects for by-production of rare earths during uranium recovery from in-situ leaching solutions

Russia possesses huge and variable resources and reserves of rare and rare earth metals. At the same time, the existing methods of recovery of rare earth elements (REE) from the conventional sources are rather complicated, while the commercial production is impeded due to the lack of the appropriate infrastructure in the area of concentration of REE. By-production of rare earth metals at operating in-situ leaching mines with the mature infrastructure is also a promising way of REE recovery enhancement. Out of geochemical associates of hydrogenous uranium (selenium, rhenium, vanadium, molybdenum), only rhenium was extracted from process solutions. This article considers the by-production prospects of yttrium and lanthanides from pregnant solutions of in-situ leaching (ISL) during uranium production at the deposits in Khiagda ore field. Based on the lab-scale and full-scale studies, as well as the tests using a mobile research plant, the basic diagram is developed for the rare earth recovery from the in-situ uranium leaching solutions. In the long view, it is required to undertake semi-commercial trials of the proposed flowsheet for the recovery of rare earth elements from ISL solutions with production of bulk REE concentrate. The main areas of the further studies into the by-production of valuable components together with uranium from ISL solutions should be finding of new selective ion-exchangers for the preliminary concentration of valuable components (middlings), as well as effective extraction and precipitation technologies for deactivation and purification of middlings and final products. REE 227Ac recovery from concentrate improves the economic efficiency of by-production.

Modelling the Scaling-Up of the Nickel Electroforming Process

Electroforming is increasingly gaining recognition as a promising and sustainable additive manufacturing process of the “Industry 4.0” era. Numerous important laboratory-scale studies try to shed light onto the pressing question as to which are the best industry approaches to be followed towards the process’s optimisation. One of the most common laboratory-scale apparatus to gather electrochemical data is the rotating disk electrode (RDE). However, for electroforming to be successfully optimised and efficiently applied in industry, systematic scale up studies need to be conducted. Nowadays, well-informed simulations can provide a much-desired insight into the novelties and limits of the process, and therefore, scaling up modelling studies are of essence. Targeted investigations on how the size and geometry of an electroforming reactor can affect the final product could lead to process optimisation through simple modifications of the setup itself, allowing immediate time- and cost-effective adjustments within existing production lines. This means that the accuracy of results that any scaled up model provides, if compared to a successful, smaller scale version of itself, needs to be investigated. In this work a 3-D electrodeposition model of an RDE was used to conduct geometry and model sensitivity studies using a commercial software as is often done in industry. As a next step, a 3-D model of an industrial-scale electroforming reactor, which was 90 times larger in electrolyte volume compared to the RDE, was developed to compare, and identify the key model parameters during scale up. The model results were validated against experimental data collected in the laboratory for both cases to assess model validity.

Augmentation of Soil-Moisture Retention with Natural Hydrogel Extracted from Fenugreek Seed Husk for Loamy Soil with Real-Time Monitoring Using FC-28 Sensors

In the industrial era, agriculture continues to be a strong force capable of altering any nation’s economy. In this context, the inflation will strive heavily upon its agricultural produce. In the looming threat of climate change induced draughts, the issue of water scarcity and consequent depreciation of soil-moisture levels continues to haunt farmers impacting both quantity and quality of produce. The present measures which are being employed to enhance retention levels of soil-moisture are chemical-based, and could lead to environmental consequences and health hazards. This research strives for a sustainable natural solution by studying the potential of Fenugreek seed husk extract as an alternative replacement. The study strives to take advantage of the presence of mucilage with hydrophilic galactomannan within fenugreek seeds. Soil-moisture sensors enabled with Wi-Fi modules and RTC timers were employed to facilitate smart monitoring and real-time determination in the lab-scale studies for loamy soil. The comparative performance analysis with synthetic hydrogel concludes the research highlighting benevolence of natural hydrogel in enhancing soil-moisture levels.

A laboratory-scale study of residential greywater treatment with sugarcane in a constructed wetland.

Due to India's population expansion, water recycling is critical to reducing water scarcity. The purpose of this study is to discuss the recycling and reuse of domestic greywater. The horizontal subsurface flow constructed wetland (HSSF-CW) was employed to treat greywater, with bioenergy crops replacing decorative plants. CO 86032 and CO 15027 sugarcane varieties were employed for phytoremediation. In a laboratory-scale HSSF-CW system with dimensions of 0.92m, 0.61m, and 0.45m, coarse aggregate (20mm), brick jelly (20mm), and red soil mixed with coir pith (1/3 of coir pith volume-based) were employed as filter materials. During a hydraulic retention time (HRT) of 2 to 48h, the maximum removal efficiency of biochemical oxygen demand (BOD), chemical oxygen demand (COD), total suspended solids (TSS), and total nitrogen (TN) was 77.78-90%, 69.92-81.20%, 82-91.06%, and 75.83-84.02%, respectively.

Challenges and opportunities in microwave-assisted catalytic pyrolysis of biomass: A review

Microwave-assisted catalytic pyrolysis (MACP) is one of the emerging technologies for efficiently converting the solid biomass residues to valuable products, including liquid bio-oil and solid biochar. However, due to the poor dielectric properties, low thermal-conductivity, and complex compositions of biomass feedstock, two major technical challenges of biomass MACP technologies are how to heat biomass efficiently by microwave, and how to tune the pyrolysis process to improve the quality of products by catalysis. In the literature, direct, indirect as well as hybrid (localized and segregated) heating modes have been explored to improve the heating performance of microwave reactors, and in-situ, ex-situ and hybrid (dual, layered and series) catalyst configurations have been applied using different microwave absorbents and catalysts. In this review, those heating and catalysis strategies are systematically and critically reviewed (data from about 80 lab-scale studies and 1 pilot-scale unit) to reveal their differences in terms of heating efficiency and product qualities. For some biomass feedstock with high dielectric properties or under high electromagnetic field, direct heating without the use of microwave absorbent could be used. Indirect heating, on the other hand, can be used for all feedstock in which heat is transferred to the biomass via conduction/convection from high temperature surfaces heated by microwave. Hybrid heating with the use of microwave absorbents can greatly increase the local heating rate, promote the pyrolysis reaction rate and tune the distribution of pyrolytic products. In-situ catalysis shows the capability to tune the quantity and quality of both bio-oil and biochar by promoting direct interaction of catalyst and biomass via the pyrolysis vapour, while ex-situ catalysis exhibits a better potential to adjust the bio-oil yield and selectivity of desired compounds in bio-oil, and extends the catalyst life by avoiding the direct contact between biochar and catalyst. Hybrid catalytic mode combines in-situ and ex-situ catalysis in a more complex setup, but shows a great potential to regulate the yield, composition and properties of multiple products. Techno-economic-environmental assessments were also reported on the benefits of MACP processes with different heating and catalytic strategies, mostly based on the lab and limited pilot scale performance data. Future research and development should be focused on the evaluation of different absorbent-catalyst integration, heating-catalysis synergistic effect, optimization of products yields and properties, and how to scale up the MACP reactor technologies toward commercialization with continuous biomass feeding.

Sustainable Bioeconomy prospects of diatom biorefineries in the Indian west coast

The rapidly depleting fossil fuel reserves with rising greenhouse gas levels (GHGs) in the atmosphere necessitate exploring alternate sustainable energy options. Biofuels from microalgae are emerging as a viable renewable energy resource owing to their inherent characteristics of higher biomass and lipid yield per hectare compared to other terrestrial bioenergy feedstocks. In this context, the present communication highlights the prospects of microalgal biofuel and other value-added products produced in a decentralized microalgal biorefinery in the flood plains (gazani lands) of the west coast of India. The spatial extent of potential sites for diatom cultivation estimated in three districts along the Indian west coast was 1940 ha. The opportunities for establishing biorefineries using diatoms as renewable bioenergy feedstocks were investigated through species prioritization, seasonal availability, tolerance, and biochemical composition analyses. Nitzschia and Amphora sp. were prioritized for lab-scale productivity studies based on their tolerance and macromolecular composition. When cultivated in a prototype biofilms-based bioreactor designed using gravel stones as substrates, Amphora sp. Yielded 16 times more productivity (0.56 g L−1) than conventional shake flask cultures. Design of a diatom biorefinery and its mass budgeting considering 100 kg dry biomass yielded ∼15–24 kg of biodiesel. Techno-economic assessment of biodiesel with value-added products of glycerol, biogas, and biofertilizer demonstrated a biodiesel production cost of 30.08–59.52 INR/kg of biodiesel. Harvesting cost in a hybrid mode using mechanized scrubbers and manual labour was estimated as 20 INR/kg of biomass.

Toxicity and removal of pharmaceutical and personal care products: a laboratory scale study with tropical plants for treatment wetlands

Abstract The aim of the research was to evaluate the response of three tropical species (Heliconia psittacorum, Ciperus haspan, Hedychium coronarium), respect their tolerance and removal capacity of pharmaceutical and personal care products (PPCPs), namely acetylsalicylic acid, ibuprofen, methyl hydrojasmonate (cis – MDJM), galaxolide, tonalide, caffeine, naproxen, ketoprofen, and diclofenac. The study was undertaken in two stages (Stage I – Tolerance; Stage II – Removal) of 21 days each. In Stage I, it was found evidence that from 1,000 μg L−1 the plants show decaying responses, being C. haspan and H. psittacorum, the species with the best responses to tolerance and adaptation. The results of Stage II indicated that tonalide and ketoprofen compounds were 99% removed during the first 24 hours of exposure; acetylsalicylic acid, ibuprofen, galaxolide, and naproxen compounds were 80% eliminated, and caffeine and diclofenac products presented lower removal rates during same time. The study allowed the identification of two compound blocks, PPCPs that are sorbed by plants (acetylsalicylic acid, ibuprofen, MDJM, caffeine, galaxolide, and tonalide), and highly photodegradable compounds (ketoprofen, naproxen, and diclofenac). These findings open the possibility for further research about using plants adapted to tropical conditions, for PPCP removal from wastewaters in real scale nature-based systems such as treatment wetlands.

Integration of in-situ chemical oxidation and permeable reactive barrier for the removal of chlorophenols by copper oxide activated peroxydisulfate

In-situ chemical oxidation (ISCO) and permeable reactive barrier (PRB) have been used in field practices for contaminated groundwater remediation. In this lab-scale study, a novel system integrating ISCO and PRB using peroxydisulfate (PDS) as the oxidant and copper oxide (CuO) as the reactive barrier material was developed for the removal of 2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP). The influences of chlorophenol concentration and flow rate on the system performance were first evaluated using synthetic solutions. The removal efficiencies of target chlorophenols were greater than 90% when sufficient PDS was supplied ([PDS]/[chlorophenol]>1). It was also found that the removal efficiencies decreased with the increasing chlorophenol concentrations (10–150 μM) and flow rates (1.8–14.4 mL/min). When three real groundwaters were employed, the removal efficiencies of 2,4-DCP and 2,4,6-TCP slightly reduced to 90% and 85%, respectively. For PCP, the removal efficiency dropped to 20% in two groundwaters with relatively high levels of alkalinity. The influences of pH and TOC were found to be insignificant for the range investigated (pH 6.5–8.7 and TOC = 0.4–1.5 mgC/L). The reduced removal efficiency could be due to the formation of weaker radicals and the stronger competition between bicarbonate ions and PDS for the activation sites on the CuO surfaces.

New Facility for Membrane Fouling Investigations under Customizable Hydrodynamics: Validation and Preliminary Experiments with Pulsating Cross-Flow.

Flux reduction induced by fouling is arguably the most adverse phenomenon in membrane-based separation systems. In this respect, many laboratory-scale filtration studies have shown that an appropriate use of hydrodynamic perturbations can improve both performance and durability of the membrane; however, to fully understand and hence appropriately exploit such effects, it is necessary to understand the underpinning flow processes. Towards this end, in this work we propose and validate a new module-scale laboratory facility with the aim of investigating, at very well-controlled flow conditions, how hydrodynamics affects mass transport phenomena at the feed/membrane interface. The proposed facility was designed to obtain a fully developed and uniform flow inside the test section and to impose both steady and pulsating flow conditions. The walls of the facility were made transparent to grant optical accessibility to the flow. In this paper, we discuss data coming from particle image velocimetry (PIV) measurements and preliminary ultrafiltration tests both under steady and pulsating flow conditions. PIV data indicate that the proposed facility allows for excellent flow control from a purely hydrodynamic standpoint. Results from filtration tests provide promising results pointing towards pulsating flows as a viable technique to reduce fouling in membrane systems.

Evaluation of sludge discharge methodologies in aerobic granular sludge reactors

We performed a comparison of different aerobic granular sludge (AGS) discharge methodologies in sequencing batch reactors (SBRs), evaluating the system efficiency, physical and microbiological characteristics of granules, and resource recovery of the discharged biomass. In R1, there was a selective discharge (30% top sludge and 70% bottom sludge, based on biomass weight) with SRT control (10–20 days). The same selective discharge was applied to R2, but with control of food to microorganisms (F/M) ratio (0.22–0.44 gCOD/gVSS·day). In R3, the discharge was conventional (similar to activated sludge reactors), i.e., mixed liquor during the aerobic period. Additionally, the best protocol of the lab-scale studies was used in a pilot-scale reactor (R4) treating municipal wastewater. R1 protocol was the best strategy for AGS formation, maintenance, and performance, achieving better removal efficiencies of COD (95%), total nitrogen (85%), and phosphorus (80%). Sludge discharge improved the system's performance and resource recovery, especially alginate-like exopolysaccharides (ALE).

A lab-scale study on the influence of the compost-dewatering process on moisture removal and pathogen inactivation in pre-sanitized fecal sludge

Abstract In many developing cities, fecal sludge management has become a serious environmental issue. As the increasing urban settlement results in the generation of fecal matter that causes environmental problems, recovering and recycling fecal waste for soil amendment could be beneficial. In this study, pre-sanitized fecal sludge (FS) was collected from the ongoing FS treatment process at the University of Science and Technology Beijing and subjected to a compost-dewatering process to mitigate pathogen and moisture content (MC). Biochar and dry leaves were added to reactors 1, 2, and 3 (1:1, 2:1, and 3:1) at 10% to facilitate the degradation process. The result shows that the final MC from the 45-day experiment has values of 35.1, 37.3, 38.9, and 65% in reactors 1, 2, 3, and the control, respectively. The indicator organism (fecal coliform) was completely mitigated in reactors 1 and 2. However, fecal coliform was merely reduced from 7.2 to 5.7 log10 CFU/100 mL in reactor 3 and remained available in the control reactor. This phenomenon of pathogen inactivation and MC removal from FS was attributed to the concentration of lactic acid bacteria (LAB) in the pre-sanitized FS. The addition of LAB to the treatment process enhanced the acidification process and resulted in pathogen inactivation. Biochar and dry leaves also played an important role in mitigating moisture and enhancing the fast composting process. Given the hygienic condition of the compost, it is suitable for soil amendment in agriculture.

Preparation of a Beverage Containing Freeze-Dried Watercress for a Clinical Trial of Carcinogen and Toxicant Detoxification

This study describes the preparation of a beverage containing freeze-dried watercress suitable for consumption in a clinical trial to determine whether a constituent of this beverage-PEITC, which has cancer prevention properties-can enhance detoxification of common environmental carcinogens and toxicants such as benzene, which may have a role in environmentally induced cancer. See related Spotlight, p. 139.

Fully printed and flexible multi-material electrochemical aptasensor platform enabled by selective graphene biofunctionalization

The demand for flexible biochemical sensors has increased with advances in computational functionality and wireless communication. Advances in materials science and biochemistry have enabled the development and fabrication of biosensors for selective detection of biological analytes leveraging ink-printed technologies, including in flexible form-factors. However, despite these advances, minimal effort has been devoted to translating the multi-material, three-electrode electrochemical cell, which is widely regarded as the standard for laboratory-scale studies, into a flexible form-factor for use in immunosensors, especially in a manner that is compatible with rapid and scalable additive manufacturing. Here, we report a fully printed and flexible electrochemical non-enzymatic immunosensor platform that integrates four chemically compatible inks and a non-covalent, two-step biofunctionalization scheme. The robustness of the platform is demonstrated using a model aptasensor that enables lysozyme detection using both electrochemical impedance spectroscopy and square wave voltammetry. The flexible, fully ink-printed aptasensor shows competitive performance to commercially available rod/disc electrodes in a bath cell. Overall, this work establishes a methodology for high-throughput fabrication of robust, flexible, multi-material, three-electrode immunosensors that can be generalized to a range of biosensor applications.

Study on Nitrogen Transfer in the Symbiotic Ecosystem between Tubificidae and Microbes

A lab-scale study of the symbiotic ecosystem between Tubificidae and microbes (SETM) was carried out. The sludge reduction ability and ammonia removal performance of the system were investigated, and the nitrogen transfer pathway in the system was studied. In SETM, the substrates, microbes, and Tubificidae interacted with each other and reached equilibrium. During the experimental period (50 days), the amount of SETM sludge reduced by 30% compared to the control group (without Tubificidae). A considerable amount of nutrients was released in the presence of Tubificidae. Conceptual models of nitrogen flow were proposed, and the nitrogen transfer in the systems was described. In SETM, 21% more nitrogen was removed via assimilation compared with the control group, and the net nitrogen removal (including endogenous nitrogen) was higher than in the control group. The effective ammonia removal in SETM was 13% higher than in the control group. This result was supported by fluorescent in situ hybridization data showing that ammonia-oxidizing bacteria were 5% more prevalent in SETM than in the control group.

EFFECT OF TEMPERATURE ON RUMEN MICROBES ACTIVITY TO PRODUCE METHANE FROM COAL

This paper is intended to show the effect of temperature on utilization microbes of rumen Àuid to produce methane from coal (lignite, sub-bituminous and bituminous). By considering to field applications, where the CBM reservoir temperature is higher than the microbes natural living conditions in the cow's body, the experiment is carried out at a temperature of 30ºC and 50ºC with an incubation period time of 105 days. Laboratory scale study was conducted by mixing the coal, formation water and rumen Àuid (1: 1: 1). This mixture were inserted into a closed chamber equipped with a hose to measuring the volume of gas produced. The experiment results showed that the microbes of the rumen Àuid can grow on different type of coal and temperatures. The volume of gas production increased during the incubation time for all treatments. Obtained the highest methane production occurs at a temperature treatment of 50ºC for the sub-bituminous coal with a volume of 0.627liters/kg (22.14 cf/ton) from the total volume of gas produced as much as 7.25liters/kg (256 cf/ton).

THE REMOVAL EFFICIENCY OF ORGANIC LOADING, PHOSPHATES AND DETERGENTS IN WASTEWATER OF CAR WASH SERVICE BY CONSTRUCTED WETLAND SYSTEM

The car wash wastewater has a proportion of detergent  and it can cause considerable impacts to pollute the environment and health if they are not processed in well. SSVF CW is one of  an alternative wastewater treatment, because it has the advantage such as wastewater flowing below the surface of the media so it  reduces the smell of wastewater. The objective of this study was to determine the  removal efficiency of reducing pollutants in the wastewater of treated car wash by Constructed Wetland and the influence of Chrysopogon zizanioides rhizosphere depth in supplying DO distribution. This experimental laboratory scale study used 2 PVC for reactor. Each pipe has 4 sampling points. The DO was measured from different depth, while the other parameters were from the effluent. The ratio of the media used was  1:3:2:4 from the top to the bottom. The SSVF CW system had high TSS removal efficiency of 94.74%, while the removal efficiency of COD, Detergent, and Phosphate was76.21%, 54.54%, 30.65%, respectively. DO distribution at a depth of 84 cm in both pipes increased even though the root zone was only 26.5 cm. Â

A Hybrid Experimental and Theoretical Approach to Optimize Recovery of Rare Earth Elements from Acid Mine Drainage Precipitates by Oxalic Acid Precipitation

The development of processing techniques for the extraction of rare earth elements and critical minerals (REE/CM) from acid mine drainage precipitates (AMDp) has attracted increased interest in recent years. Processes under development often utilize a standard hydrometallurgical approach that includes leaching and solvent extraction followed by oxalic acid precipitation and calcination to produce a final rare earth oxide product. Impurities such as Ca, Al, Mn, Fe and Mg can be detrimental in the oxalate precipitation step and a survey of the literature showed limited data pertaining to the REE precipitation efficiency in solutions with high impurity concentrations. As such, a systematic laboratory-scale precipitation study was performed on a strip solution generated by the acid leaching and solvent extraction of an AMDp feedstock to identify the optimal processing conditions that maximize REE precipitation efficiency and product purity while minimizing the oxalic acid dosage. Given the unique chemical characteristics of AMDp, the feed solution utilized in this study contained a moderate concentration of REEs (440 mg/L) as well a significant concentration (>7000 mg/L total) of non-REE contaminants such as Ca, Al, Mn, Fe and Mg. Initially, a theoretical basis for the required oxalic acid dose, optimal pH and predicted precipitation efficiency was established by solution equilibrium calculations. Following the solution chemistry calculations, bench-scale precipitation experiments were conducted and these test results indicate that a pH of 1.5 to 2, a reaction time of more than 2 h and an oxalic acid dosage of 30 to 40 g/L optimized the REEs recovery of at ~95% to nearly 100% for individual REE species. The test results validated the optimal pH predicted by the solution chemistry calculations (1.5 to 5); however, the predicted dosage needed for complete REE recovery (10 g/L) was significantly lower than the experimentally-determined dosage of 30 to 40 g/L. The reason for this discrepancy was determined to be due to the large concentration of impurities and large number of potential metal complexes that cause inaccuracies in the solution equilibrium calculations. Based on these findings, a hybrid experimental and theoretical approach is proposed for future oxalic acid precipitation optimization studies.

Revealing the mechanisms of irreversible fouling during microfiltration – The role of feedwater composition

While microfiltration has seen wide acceptance in water treatment, the irreversible fouling of microfilters remains as the biggest impediment to their long-term performance. Thus, developing a better understanding of the mechanisms of irreversible fouling is crucial. To this end, we investigated the mechanism of effluent organic matter (EfOM)-membrane interactions responsible for irreversible fouling of polyvinylidene fluoride (PVDF) microfiltration membranes during wastewater effluent filtration by evaluating the backwash efficacy of different backwashing solutions (ultrapure water, NaCl, CaCl2, HCl, NaOH, and NaClO). The backwash efficiency followed the order HCl < salt solution ≈ Ultrapure water < NaOH < NaClO. A combined analysis of these results suggests that electrostatic interactions (divalent cation influenced EfOM-membrane bridging) play a minimal role in effecting irreversible fouling and non-electrostatic direct EfOM-membrane interactions like hydrophobic interactions dominantly influence irreversible fouling. Since model organic matter species (bovine serum albumin, alginate, and humic acid) are regularly used to simulate wastewater effluent, we also evaluated the mechanism of irreversible fouling during filtration of model organic matter solution using salt solution backwashing. The efficiency followed the order – CaCl2 < Ultrapure water < NaCl, indicating electrostatic interactions as the dominant fouling mechanism. Thus, results from lab-scale studies employing model organic matter species should be interpreted cautiously and conservatively.

Isolation and Screening of Odor-Reducing Microbes from Swine Manure and Its Role in Reducing Ammonia Release in Combination with Surfactant Foam

Swine farming facilities have increased the production of malodorous gases, which negatively affects people. Hence, we developed a new feasible bio-foam technology wherein long-lasting surfactant foam, including bacteria, were sprayed on swine manure. The surfactant foam acted as a physical barrier, suppressing NH3 release, and the aqueous-phase bacteria formed after foam breaking infiltrated in manure and degraded NH3. In this study, we first isolated NH3-degrading bacteria from swine manure. A bacterial consortium was prepared using the effective NH3-degrading strains Saccharomyces cerevisiae NRRL Y-12632 (99.88%) (TP1), Lactococcus lactis subsp. hordniae NBRC100931T (99.93%) (TP3), and Lactobacillus argentoratensis DSM 16365T (100%) (TP5). The surfactant foam used in this study was a dry foam (foam quality 98.5–99.0% and foam density 0.025–0.026 g/cm3), with a foam expansion of 110–112 and high foamability. Large bubbles were generated with a bubble density of 1 bubble/cm2 and a foam lamella thickness of 0.12 mm. In a lab-scale study, foam was sprayed onto NH3-contaminated soil or real swine manure, which reduced the NH3 emission from the source (soil/manure) almost completely (97–100%), but NH3 was re-emitted after foam breaking (5 h: open reactor, 7 h: closed reactor). After loading the bacteria on the foam, the initial NH3 odor suppression was similar to that of the foam alone. However, NH3 was effectively reduced by microbial degradation even after foam breaking. Complete odor degradation was observed after 3 days (72 h; 90–100% reduction) for the NH3-contaminated soil, and 97.7% NH3 in the swine manure was reduced in 24 h. Furthermore, the reagent cost for preparing stable foam was reasonable, indicating its possible field extension.