Laboratory Preparation Research Articles

The quantum pencil revisited

A quantum description is used to find the time limit for balancing an idealized pencil-like object on end. In agreement with some prior studies, analysis for this system shows that quantum effects are not observable in any foreseeable circumstances, either by casual experimentation or by extensive laboratory preparation. A direct solution of Schrodinger’s equation avoids an a priori use of the Heisenberg uncertainty relation in the classical equations of motion, which, although very much a proper usage, may lead to misunderstanding of the results. The classical equations of motion are adequate for the pencil in all circumstances.

Insights into human history from the first decade of ancient human genomics

Recent advancements in DNA sequencing technologies and laboratory preparation protocols have rapidly expanded the scope of ancient DNA research over the past decade, both temporally and geographically. Discoveries include interactions between archaic and modern humans as well as modern human population dynamics, including those coinciding with the Last Glacial Maximum and the settlement history of most world regions. This new type of data allows us to examine the deep past of human population dynamics and sharpen the current understanding of our present. The continued development in the ancient DNA field has transformed our understanding of human genetic history and will keep uncovering the further mysteries of our recent evolutionary past.

English

Removable prosthesis is probably one of the oldest types of dental therapy used to rehabilitate patients with complete or partial edentulousness. The main function of the edentulous alveolar ridge is to provide a major area of support for the dentures.1 Ill-fitting prosthesis leads to a condition where there is no longer adequate support of bone due to RRR. Hence, rehabilitation of such cases pose a clinical challenge, as there is a decreased denture foundation area for support, retention and stability.2 Residual ridge resorption (RRR) is the severe atrophy of alveolar bone underlying the mucoperiosteum. RRR affects the alveolar bone morphology, resulting in a decreased denture foundation area and increased inter-alveolar ridge space. The increase in inter-ridge distance provides a huge restorative space which results in a heavier complete denture due to greater volume of denture base material. This increase in restorative space amalgamates with the long lip length of the patient which gives rise to huge challenge to the success of the complete denture. Also, decreased denture foundation area increases the heaviness of the prosthesis and negatively affects the retention of the denture due to gravity.3 Various weight-reduction techniques have been used in the past using a rigid three-dimensional spacer, such as dental stone (Ackerman, 1955), cellophanewrapped asbestos, silicone putty during laboratory preparation to keep denture base content out of the intended hollow cavity of the prosthesis. The following case report describes a distinct lab technique for construction of hollow maxillary complete denture for a patient with severe RRR.

Danish national guideline for the treatment of Clostridioides difficile infection and use of faecal microbiota transplantation (FMT)

Aim: This Danish national guideline describes the treatment of adult patients with Clostridioides (formerly Clostridium) difficile (CD) infection and the use of faecal microbiota transplantation (FMT). It suggests minimum standard for implementing an FMT service. Method: Four scientific societies appointed members for a working group which conducted a systematic literature review and agreed on the text and recommendations. All clinical recommendations were evalluated for evidence level and grade of recommendation. Results: In CD infection, the use of marketed and experimental antibiotics as well as microbiota-based therapies including FMT are described. An algorithm for evaluating treatment effect is suggested. The organisation of FMT, donor recruitment and screening, laboratory preparation, clinical application and follow-up are described. Conclusion: Updated evidence for the treatment of CD infection and the use of FMT is provided.

Swelling pressure of compacted MX80 bentonite/sand mixture prepared by different methods

Compacted bentonite/sand mixture have been considered as possible sealing/backfilling material in the deep geological disposal of high-level radioactive nuclear waste. The swelling pressure of the compacted bentonite/sand mixture is of significance in the design of the geological repository, which requires good consistency between data obtained by laboratory and field measurement. In this work, a series of swelling pressure tests were performed on compacted MX80 bentonite/sand specimens prepared by methods commonly adopted in laboratory (As-compacted, Transferred) and those mimicking the real block manufacturing process (Trimmed, Inserted). Results shown that with identical dry density (especially when the dry density was larger than 1.70 Mg/m3), largest swelling pressure was obtained in specimens prepared by method Inserted, followed by method As-compacted, Transferred and Trimmed. The distinct difference between the swelling pressure could be largely attributed to the effects of residual post-compaction lateral stress. More interestingly, specimens prepared by methods Trimmed and Transferred followed a similar swelling pressure-dry density relationship. From this point of view, method Transferred without causing mass loss, change of bentonite content and possible technological voids effect was recommended in lieu of method Trimmed for specimen preparation in laboratory.

Standartization of Forensic Activities and Certification of Expert Laboratories as a Factor for the Development of Forensic Examination

The author has carried out the analysis of the perspectives for certification of domestic forensic laboratories for the compliance with international standards, which would contribute to the creation of an effective legal mechanism for the transition to a new level of development of forensic technologies. The main factors of administrative and legal regulation in the field of standardization have been studied. In accordance with the requirements of DSTU ISO / IEC 17025 the principles of organization and implementation of the quality management system (QMS) into forensic laboratories have been determined.
 It has been established that the execution of the DSTU ISO / IEC 17025 requirements needs significant resources and financial costs from the laboratory. The author has provided clarification to the main stages of preparation for certification, which include measures on developing management documentation (Guidelines, Regulations on forensic laboratory, Accreditation Area), on inventory of existing equipment, metrological support of laboratory activities, preparation and submission of applications to the accreditation agency. It has been noted that certified forensic laboratories should use certified expert methods within their activities that can ensure reliable results during the research. Methodologies should be tested in other reputable organizations or validated directly in the laboratory. Considerable attention has been paid to the quality of standard samples, reagents and materials. The emphasis has been placed on the need to participate in proficiency testing programs based on the results of interlaboratory comparative tests as an effective way to monitor the activities of the forensic laboratory.
 The author has substantiated the conclusion that the standardization processes of forensic activity and certification of forensic laboratories are aimed at increasing the level of metrological work to protect citizens and the national economy of Ukraine from the consequences of unreliable measurement results.

Use of Propionibacterium freudenreichii T82 Strain for Effective Biosynthesis of Propionic Acid and Trehalose in a Medium with Apple Pomace Extract and Potato Wastewater.

Propionic acid bacteria are the source of many metabolites, e.g., propionic acid and trehalose. Compared to microbiological synthesis, the production of these metabolites by petrochemical means or enzymatic conversion is more profitable. The components of microbiological media account for a large part of the costs associated with propionic fermentation, due to the high nutritional requirements of Propionibacterium. This problem can be overcome by formulating a medium based on the by-products of technological processes, which can act as nutritional sources and at the same time replace expensive laboratory preparations (e.g., peptone and yeast extract). The metabolic activity of P. freudenreichii was investigated in two different breeding environments: in a medium containing peptone, yeast extract, and biotin, and in a waste-based medium consisting of only apple pomace and potato wastewater. The highest production of propionic acid amounting to 14.54 g/L was obtained in the medium containing apple pomace and pure laboratory supplements with a yield of 0.44 g/g. Importantly, the acid production parameters in the waste medium reached almost the same level (12.71 g/L, 0.42 g/g) as the medium containing pure supplements. Acetic acid synthesis was more efficient in the waste medium; it was also characterized by a higher level of accumulated trehalose (59.8 mg/g d.s.). Thus, the obtained results show that P. freudenreichii bacteria exhibited relatively high metabolic activity in an environment with apple pomace used as a carbon source and potato wastewater used as a nitrogen source. This method of propioniate production could be cheaper and more sustainable than the chemical manner.

The application of reusable learning objects (RLOs) in preparation for a simulation laboratory in medication management: An evaluative study

To enhance the preparedness of undergraduate nursing and midwifery students to participate in the safe provision of medication administration on their clinical placements, an innovative blended learning strategy was designed and developed by the authors. The blended learning strategy included a suite of online reusable learning objects specific to medication management theoretical knowledge and psychomotor skills to prepare students for a 90-minute practical face to face simulation laboratory session. Students identified that the reusable learning objects had prepared them for the simulation laboratory session and was rated as a productive learning experience. The blended learning strategy implemented to teaching and learning medication management to undergraduate nursing and midwifery students can positively influence students’ acquisition of knowledge and psychomotor skills to safely administer medications prior to their practice placements in a clinical setting.

Comparison of antioxidant activity and extraction techniques for commercially and laboratory prepared extracts from six mushroom species

Mushrooms are a potent source of dietary antioxidants and are often marketed for their capability of decreasing oxidative stress and preventing diseases. To date, however, little research has been done on the antioxidant activity of commercially available mushroom extractions, or their stability over time. Herein, the antioxidant capacity of six commercially available mushroom tinctures (hydro-alcoholic extracts of chaga, maitake, shiitake, reishi, lion's mane, and turkey tail) were evaluated using a panel of five antioxidant assays: Oxygen Radical Absorbance Capacity (ORAC), Nanoceria Reducing Antioxidant Capacity (NanoCerac), DPPH radical scavenging, Total Phenolic Content (TPC), and Ferric Reducing Antioxidant Power (FRAP). Commercial samples were compared to our own laboratory preparations of the same species, in a preliminary assessment of extraction technique and antioxidant stability. Mushroom extracts exhibited different antioxidant capacities depending on species and extraction method (e.g., 0.023 to 0.63 μmol TE/mg for commercial ORAC vs. 0.05 to 1.8 μmol TE/mg for laboratory ORAC for all species). In all cases, laboratory extracts exhibited considerably higher antioxidant activities than commercial extracts. In both extract types, chaga and maitake consistently demonstrated higher antioxidant activity than the other mushrooms. Preliminary antioxidant stability testing of our samples revealed stable ORAC values for one month at room temperature, followed by a 48% decrease after 4 months. Our results provide important insights regarding the effects of extraction techniques and storage conditions, on the antioxidant activity in mushroom extracts, particularly those made and sold commercially.

Field-Portable Microplastic Sensing in Aqueous Environments: A Perspective on Emerging Techniques.

Microplastics (MPs) have been found in aqueous environments ranging from rural ponds and lakes to the deep ocean. Despite the ubiquity of MPs, our ability to characterize MPs in the environment is limited by the lack of technologies for rapidly and accurately identifying and quantifying MPs. Although standards exist for MP sample collection and preparation, methods of MP analysis vary considerably and produce data with a broad range of data content and quality. The need for extensive analysis-specific sample preparation in current technology approaches has hindered the emergence of a single technique which can operate on aqueous samples in the field, rather than on dried laboratory preparations. In this perspective, we consider MP measurement technologies with a focus on both their eventual field-deployability and their respective data products (e.g., MP particle count, size, and/or polymer type). We present preliminary demonstrations of several prospective MP measurement techniques, with an eye towards developing a solution or solutions that can transition from the laboratory to the field. Specifically, experimental results are presented from multiple prototype systems that measure various physical properties of MPs: pyrolysis-differential mobility spectroscopy, short-wave infrared imaging, aqueous Nile Red labeling and counting, acoustophoresis, ultrasound, impedance spectroscopy, and dielectrophoresis.

In situ carbon and oxygen isotopes measurements in carbonates by fiber coupled laser diode-induced calcination: A step towards field isotopic characterization

Natural stable isotopes ratios (δ13Ccarb and δ18Ocarb) of carbonates archived in the geological record are routinely used to reconstruct local and global paleo temperatures and the secular evolution of the biogeochemical carbon cycle. The state-of-the-art technique, employed since the mid 20th century, to measure these isotopic ratios starts with field sampling followed by several steps of physical and chemical laboratory preparation including: (i) microdrilling and/or sawing and crushing, (ii) CO2 release by wet acid digestion, (iii) gas equilibration, purification and transfer, before (iv) gas phase IRMS measurements. While these steps are time and resource consuming, they provide accurate measurements of δ13Ccarb, δ18Ocarb and carbonate contents. This study presents a new protocol involving a compact and modernized laser calcination system that decreases drastically the analyses time by reducing the number of preparations steps together with offering the possibility of performing spatially resolved analysis at the mm scale. This new method is based on the use of a fiber coupled laser diode device emitting 30 W in the near infrared at 880 nm. The energy provided by the laser source induces the decomposition of calcium carbonate into lime and carbon dioxide. In this work, the CO2 was collected in sample tubes under a controlled atmosphere for offline analysis, however additional developments should permit online analysis in the near future.We analyzed 9 different types of carbonate minerals encompassing a range of isotopic compositions VPDB between +3.3 and − 18.2‰ and between −1.7 and − 14.6‰ for δ13Ccarb and δ18Ocarb, respectively. A comparison of isotopic results was performed for carbonate zones analyzed both by classic methods (micro-drilling followed by acid digestion) and laser calcination. This isotopic cross-calibration exercise shows a direct positive co-variation between both methods with a correlation coefficient of 0.99 and a regression slope of 1 within uncertainties for the δ13Ccarb values. The δ18Ocarb values also compared well with a correlation coefficient of 0.96, suggesting a constant gas-solid phase isotopic equilibrium between carbon dioxide and lime. The reproducibility of our laser calcination method performed on replicate analyses of dolomite, siderite and malachite shows a 1σ standard deviation of 0.31 and 0.77 for δ13Ccarb and δ18Ocarb, respectively. These reproducibilities are within the observed isotopic natural inhomogeneity of samples (up to 1.3 and 0.57‰ for the δ13Ccarb and δ18Ocarb, respectively) as assessed by microdrilling and acid digestion.Based on the suit of samples analyzed in this study, we demonstrate that (i) fiber coupled laser diode calcination enables accurate and reproducible C and O isotopic characterization of natural carbonates, (ii) physical effects during calcination do not introduce any isotopic fractionation for C and is accompanied by a constant isotopic offset for O over a range of isotopic compositions and mineral matrices. These findings pave the way for a new range of possibilities for carbonate δ13C and δ18O measurements directly in the field using rapid, portable, and easy to manipulate laser preparation devices paired with CRDS/IRIS optical-mass spectrometers.

Unmasking the Structure of Gross Anatomy Laboratory Sessions During Covid‐19

INTRODUCTION/OBJECTIVECovid‐19 created immediate challenges to anatomy education. The traditional format of gross laboratory sessions experienced a direct impact and few reports documented curricular delivery changes specific to laboratory format. The purpose of this study was to assess the adaptations incorporated in gross anatomy laboratories by anatomists, during May‐August 2020, in response to Covid‐19.MATERIALS/METHODSData were collected through the IRB‐approved Virtual Anatomy During Covid‐19 survey that consisted of 20 questions, including open‐ended prompts asking participants to describe the structure of a “typical” laboratory session during Covid‐19. Responses were solicited from professional anatomy associations during June 2020. Open‐ended responses describing anatomy laboratory teaching methods used during Covid‐19 were coded. Descriptive codes were applied to the data according to published methods to summarize verbatim responses. Responses were tabulated and converted to frequencies and percentages. Chi square test assessed differences among the responses when applicable. Alpha<5%.RESULTSDescriptions of gross anatomy lab teaching during Covid‐19 were coded into four categories : (1) delivery format, (2) format of laboratory practice, (3) type of anatomy digital resources used, and (4) format of student teaching groups. In the first category, synchronous (46.7%), asynchronous (15.6%), and/or a combination of the two (18.8%) were the most frequent laboratory delivery formats (P<0.001). In the second category, student‐led dissection (17.2%), prosection (10.9%), and/or utilization of commercial and/or in‐house anatomical resources (26.2%) were the most frequent laboratory practices (P<0.001). Within this category, a subcategory was discovered in which physical distancing and personal protective equipment practices were reported (15.6%). Concerning the third category, anatomy digital resources (26.2%) were used for asynchronous laboratory preparation and laboratory sessions. In the final category, student small groups (29.7%) were used in remote sessions where “breakout rooms” permitted students to meet with peers and/or faculty. Large groups (9.4%) were used for faculty to review and present the assigned laboratory topic.CONCLUSIONAnatomists largely taught through a remote, synchronous delivery format that relied on cadaveric specimens and digital anatomy resources, as well as small group learning.SIGNIFICANCE/IMPLICATION: This study shows that anatomists were able to adapt the gross anatomy laboratory sessions to synchronous, virtual mediums; however, the impact of these changes to the learner during this unconventional time remains to be determined.

PREVALENCE OF MICROBIOTA ASSOCIATED TO VAGINAL DISCHARGE AMONG REPRODUCTIVE AGE GROUP BASED ON SOCIOECONOMIC STATUS AND DEMOGRAPHIC BACKGROUND

In women of reproductive age, vaginal discharge is one of the most frequent signs. Vaginal discharge may be physiological or pathological; thus, this study was conducted to ascertain the microbial diversity of vaginal discharge cases in relation to socioeconomic status and demographic history among women of reproductive age attending a tertiary care hospital. To diagnose the organism, simple wet laboratory preparation, 10% KOH, Gram staining, and pH paper were used, while to classify and categorise the pathogens, various differential agar media such as CHROM agar, Blood agar, and Sabouraud Dextrose agar were used, followed by some biochemical tests and the use of the Nugent Score. The majority of the patients were between the ages of 22 and 28. The patients were all from the lower middle class 41. (37.61 percent ). Microbial diagnosis revealed that 45 percent of patients had Bacterial Vaginosis, 41 percent had Candidiasis, 5% had Trichomoniasis, and 9% had both Bacterial Vaginosis and Candidiasis..

Laboratory preparation of monochloramine for environmental research: A comparison of four commonly used protocols

Monochloramine (MCA) is often used as a secondary disinfectant in drinking water distribution systems (DWDS) for controlling the regrowth of microorganisms. Laboratory experiments to study the efficacy of MCA and formation of disinfection byproducts in DWDS all require the preparation of MCA. Different protocols for the preparation of MCA have been used in the literature. In this study, we compared four published protocols that are frequently used to prepare MCA in the environmental research community. Our results showed that the choice of protocol could result in substantial differences in decay kinetics and disinfection efficacy of the prepared MCA. The discrepancies highlight an immediate need for the standardization of MCA preparation protocols for laboratory-scale drinking water research.

Laboratory preparation of fruit, vegetable wine and physicochemical study comparison

Study mainly focused on the process monitoring of homemade wine during its fermentation period. The experimental investigation was aimed to study the variation in each Parameter during the fermentation period. The final analysis of wine of various parameters-alcohol content, pH specific gravity were conducted. These studies were compared with the Apple, Beetroot, Wine. The study concludes that pH showed a decreasing trend and then attains minimize then increases. The sugar concentration of wine decreases with increasing in the number of days. It has been studied that as the number of day’s passes, the specific gravity and volume percentage of alcohol also increases gradually. The Titrable acidity of wine showed a fluctuating trend as the number of days passes. Apple showed a pH range of 4.6 - 2.4,viscosity showed the thickness of the wine 0.17 specific gravity ranges from 1.9-1.09 and alcohol content was 14.7.Beetroot showed a pH range of 5.4-2.1,viscosity showed the thickness of the wine 1.2-0.12, specific gravity ranges from 1.93-1.06 and alcohol content was 12.3. The results of process monitoring and final analysis the physi cochemical parameters both wines having approximately equal values. But beetroot wine having low alcohol content so it would be more potent then apple wine.

Entry and Disposition of Zika Virus Immune Complexes in a Tissue Culture Model of the Maternal-Fetal Interface.

Zika virus (ZIKV) infections have been associated with an increased incidence of severe microcephaly and other neurodevelopmental disorders in newborn babies. Passive immunization with anti-ZIKV neutralizing antibodies has the potential to become a feasible treatment or prophylaxis option during pregnancy. Prior to clinical use, such antibodies should be assessed for their ability to block ZIKV passage to the fetus. We used human placental and mammalian cell monolayers that express FcRn and laboratory preparations of anti-ZIKV antibodies as a model system to investigate the disposition of ZIKV/antibody immune complexes (ICs) at the maternal-fetal interface. We further characterized solution properties of the ICs to evaluate whether these are related to in vitro effects. We found that both ZIKV and ZIKV envelope glycoprotein can enter and passage through epithelial cells, especially those that overexpress FcRn. In the presence of ZIKV antibodies, Zika virus entry was bimodal, with reduced entry at the lowest (0.3–3 ng/mL) and highest (µg/mL) antibody concentrations. Intermediate concentrations attenuated inhibition or enhanced viral entry. With respect to anti-ZIKV antibodies, we found that their degradation was accelerated when presented as ICs containing increased amounts of ZIKV immunogen. Of the two monoclonal antibodies tested, the preparation with higher aggregation also exhibited higher degradation. Our studies confirm that intact Zika virus and its envelope immunogen have the potential to enter and be transferred across placental and other epithelial cells that express FcRn. Presence of anti-ZIKV IgG antibodies can either block or enhance cellular entry, with the antibody concentration playing a complex role in this process. Physicochemical properties of IgG antibodies can influence their degradation in vitro.

Spray Drying of Bottle Gourd Juice: Effect of Different Carrier Agents on Physical, Antioxidant Capacity, Reconstitution, and Morphological Properties

In this study, the effects of different carrier agents like maltodextrin, whey protein isolate, and soy protein isolate on the laboratory preparation of spray-dried bottle gourd powder were studied...

Evaluation of the effectiveness of VOC-contaminated soil preparation based on AHP-CRITIC-TOPSIS model

Currently, several methods have been adopted for the laboratory preparation of artificial volatile organic compound (VOC) contaminated soils (VCSs). However, it remains unclear whether the prepared contaminated soils are homogenous. In this study, two representative VOCs, toluene and perchloroethylene, were separately mixed with a kaolin-based soil using six preparation methods. Thereafter, the homogeneity and recovery of the contaminated kaolin prepared using these methods were determined and analyzed. The six procedures were quantitatively assessed according to the comprehensive evaluation mathematical model (AHP-CRITIC-TOPSIS), and the final score order of the different procedures was: A > C > E > B > F > D. Additionally, the qualitative evaluation of the procedures was performed based on the phase transformation and mass transfer during the mixing processes. Based on these discussions, method A, which was considered to be optimal, was then adopted for further investigations with various natural soils. The results showed that this optimal method could be applied to natural soils and revealed that the adsorption-related characteristics of natural soils, including total organic carbon, specific surface area, pore volume, pH, plastic limit, particle size, and mineral composition, influenced the homogeneity and recovery through mass transfer. In addition, it was also observed that the chemical properties of VOCs, including molecular structure, vapor pressure, and the octanol/water partition coefficient, could also affect the effectiveness of sample recovery. Through this study, researchers can prepare VCSs with excellent homogeneity and low loss rates to conduct standardized tests for technology development.

Box-Behnken Assisted Validation and Optimization of an RP-HPLC Method for Simultaneous Determination of Domperidone and Lansoprazole

A highly specific, accurate, and simple RP-HPLC technique was developed for the real-time quantification of domperidone (DOMP) and lansoprazole (LANS) in commercial formulations. Chromatographic studies were performed using a Luna C8(2), 5 μm, 100Å, column (250 × 4.6 mm, Phenomenex) with a mobile phase composed of acetonitrile/2 mM ammonium acetate (51:49 v/v), pH 6.7. The flow rate was 1 mL·min−1 with UV detection at 289 nm. Linearity was observed within the range of 4–36 µg·mL−1 for domperidone and 2–18 µg·mL−1 for lansoprazole. Method optimization was achieved using Box-Behnken design software, in which three key variables were examined, namely, the flow rate (A), the composition of the mobile phase (B), and the pH (C). The retention time (Y1 and Y3) and the peak area (Y2 and Y4) were taken as the response parameters. We observed that slight alterations in the mobile phase and the flow rate influenced the outcome, whereas the pH exerted no effect. Method validation featured various ICH parameters including linearity, limit of detection (LOD), accuracy, precision, ruggedness, robustness, stability, and system suitability. This method is potentially useful for the analysis of commercial formulations and laboratory preparations.

Microbiological Evaluation of Laboratory Prepared Suya and Vendor Samples in Ilaro, Ogun State Nigeria

Aims: Suya is a popular spicy roasted meat product in Nigeria. The aim of the work is to investigate the microbial safety and effect of Lactobacillus species on some pathogens when used as a biopreservative on suya.&#x0D; Methodology: Five suya group samples were used. Two samples were prepared in the laboratory, one of them was inoculated with Lactobacillus plantarum while the other was un-inoculated. Three other group samples coded C, D and E were bought from different vendors at different locations in Ilaro. All the samples were subjected to microbial analysis using standard method.&#x0D; Results: The result of microbiological analysis shows that significant difference exist in all the samples. Total viable counts of the samples ranged from 16.0 ±2.5 x 103 cfu/g to 50.0 ±1.5 x 103 cfu/g, coliform counts ranged from 8.0±3 x 103 cfu/g to 44 ±3.0 x 103 cfu/g, Staphylococcus counts ranged from 10.0 ± 2.0 x 103 cfu/g to 45.5 ±3.5 x 103 cfu/g, and fungi counts ranged from 10.0 ± 2.5 x 103 cfu/g to 34.02 ±4.0 x 103 cfu/g, while Salmonella had no growth. The prepared samples harbored Streptococcus spp., Pseudomonas spp. and Staphylococcus spp. Four bacteria and three fungi species were isolated from vendor suya meat. The isolates were Staphylococcus spp., Pseudomonas spp., Escherichia coli and Streptococcus spp., while the fungi were Rhizopus spp, Penicillium spp. and Saccharomyces spp.&#x0D; Conclusion: The sample inoculated with Lactobacillus plantarum had the lowest microbial count; however, the isolation of potential pathogens from suya samples is of public health significance.