Nutrition Laboratory Research Articles

PRODUKSI DAN KANDUNGAN NUTRIEN HIJAUAN RUMPUT Pennisetum purpureum cv Mott, DENGAN PEMBERIAN PUPUK ORGANIK CAIR HASIL VERMICOMPOSTING LIMBAH SAPI POTONG

Fertilization is a very important effort for Dwarf Elephant Grass (Pennisetum purpureum cv Mott) to achieve forage production and high nutritional quality following its potential. This research aims to determine the yield of dry matter, crude protein content, and crude fiber of Dwarf Elephant Grass by application of liquid organic fertilizer (LOF) resulting from vermicomposting of beef cattle waste. The research was conducted at the Field Laboratory of Forage Crop, and the Ruminant Animal Nutrition and Feed Chemistry Laboratory, Faculty of Animal Husbandry, Padjadjaran University from January to June 2018. The research method used was the experimental method with a Completely Randomized Design using 3 treatments, namely P1 (5,000 L per Ha), P2 (10,000 L per Ha), and P3 (15,000 L per Ha), and 6 replications. Data were processed by analysis of variance and continued with Duncan's Multiple Range Test. The results showed that giving LOF increased the production, increased the content of crude protein, and decreased the crude fiber content of Dwarf Elephant Grass forage. Giving LOF from vermicomposting beef cattle waste at a dose of 15,000 L per Ha resulted in the highest forage dry matter yield (1,925 kg per Ha), one-time cutting at dry season, and the best nutrient content seen from the crude protein content (13.95%) and crude fiber content (28.53%).

EFFECTIVENESS OF LIQUID NPKSi AND N, P, K FERTILIZER ON NUTRIENT UPTAKE AND YIELD OF RICE PADDY IN ULTISOLS

Agricultural intensification and extensification need to be done to increase rice productivity. Silicon (Si) is a non-essential nutrient that plays a positive role in growth and yield. The application of liquid NPKSi fertilizer increases nutrient availability, particularly on Ultisols. The purpose of this study was to assess the effectiveness of liquid NPKSi fertilizer and straight fertilizer on nutrient uptake and yield of paddy rice on Ultisols. This research was conducted from September 2023 to June 2024 at the Soil Fertility and Plant Nutrition Experimental Farm and the Soil Chemistry and Plant Nutrition Laboratory. This study used a Randomized Block Design (RBD), which consisted of nine treatments and three replications. The treatments included control, recommended N, P, and K fertilizers, and various doses of liquid NPKSi fertilizer and straight fertilizer. The results showed that liquid NPKSi fertilizer and straight fertilizer were effective in increasing nutrient uptake and yield of paddy rice on Ultisols. The highest fertilizer effectiveness was found in the treatment of 6 L.ha-1 liquid NPKSi fertilizer and straight fertilizer (175 kg.ha-1 Urea, 37.5 kg.ha-1 SP-36, and 25 kg.ha-1KCl) with an RAE value of 218%.

POTENSI PANGAN FUNGSIONAL SUMBER VITAMIN C DAN ZINK: COOKIES DAUN KELOR DAN LABU KUNING

One of the efforts that have been made to deal with the challenge of micronutrient deficiencies is to utilize local food. Several studies have shown that the use of wheat flour from local food in the manufacture of cookies can increase the nutritional value of the product, so a study was conducted regarding the addition of moringa leaf and pumpkin flour to cookies. Analyze the content of vitamin C and zinc in cookies with the addition of moringa leaf and pumpkin flour. This study used an experimental research design which was carried out in two stages. The first stage: the manufacture of flour and cookie formulation which was carried out at the Stikes Salewangang Maros Nutritional Food Administration Laboratory. In the second stage, nutrient analysis was carried out at the Makassar Health Laboratory Center (BBLK).The results of the analysis of the content of micronutrients with 3 repetitions showed that the zinc content was 120.77 mg/g while the vitamin C was 1275.22 mg/g. So, by consuming 3 pieces of cookies can meet the daily zinc needs of children aged 6-11 months and children aged 1-3 years. Meanwhile, for vitamin C, consuming 3 pieces of cookies can meet the needs of vitamin C for children aged 1-3 years and consuming 4 pieces of cookies can meet the daily needs of children aged 6-11 months.The addition of moringa leaf flour and pumpkin flour to the cookies of this study increased the micronutrient content of vitamin C and zinc compared to cookies in general, but the results of the analysis of the content of vitamin C

Physicochemical stability, microbial growth and sensory quality of refined wheat flour as affected by packaging materials during storage

Wheat flour is a hygroscopic product requiring efficient packaging materials to maintain shelf stability and desired quality during long storage periods. This study aimed to assess the effectiveness of four packaging materials (Paper bag, Polypropylene, woven polypropylene bag + Low-density polyethylene, and Purdue Improved Crops Storage) in preserving the physicochemical, microbial, and sensory quality of refined wheat flour over 6 months at room temperature (14.8−26.80 °C). The experiment was conducted under laboratory conditions at the Postharvest Management and Animal Nutrition Laboratory of Jimma University College of Agriculture and Veterinary Medicine. Results revealed that hermetic packaging materials, specifically Purdue Improved Crop Storage (PICS) and Polypropylene bag + low-density Polyethylene (PP + LDPE), were the most effective in maintaining flour quality compared to non-hermetic options such as woven polypropylene bag (PP) and paper bag. As storage duration increased, there was a gradual decline in physicochemical quality, albeit to varying degrees depending on the packaging material. Nutritional losses were significantly lower in hermetic packaging materials. For instance, crude protein losses were 1.82% in PICS and 2.81% in PP + LDPE, compared to 4.64% in woven PP and 3.81% in paper bags. Moisture content increased by 4.43% with PP bags and 3.92% with paper bags, while the slightest changes, 1.99%, and 2.03%, were observed in PICS and PP + LDPE bags, respectively. Additionally, hermetic packaging materials reduced microbial growth. They maintained bulk density, water, and oil absorption capacity, as well as the odor and color of the stored flour compared to non-hermetic packaging materials evaluated. It is recommended that supply chain actors and consumers utilize PICS or PP + LDPE bags to ensure better physicochemical stability, microbial control, and sensory quality of refined wheat flour.

Nutritional Value and Amino Acid Profile of Dried Seeds of Cucumeropsis mannii (Cucurbitaceae)

Aims: To assess the biochemical, phytochemical and amino acid content from Cucumeropsis mannii dried seeds flours grown in Côte d'Ivoire (West Africa). 
 Study Design: Experimental.
 Place and duration of study: Sample: Laboratory of Nutrition and Food Security, Nangui Abrogoua University
 Methodology: Cucumeropsis mannii seeds were obtained for this study from an experimental field in Gagnoa (Côte d’Ivoire). In March 2022, seeds were planted and after 7 months, the mature fruits that lead to the complete drying of the plant were harvested.
 Results: The biochemical composition of the seeds revealed that they contain varying proportions of carbohydrates, fibers, ash, total sugars with contents of 9.28 ± 0.79 %; 2.72 ± 0.30 %; 3.12 ± 0.38; 2.34 ± 0.05, respectively. High levels of lipids, proteins, dry matter and energy were observed in seed flour with values of 47.46 ± 1.55 %; 31.80 ± 1.16 %; 94.38 ± 0.6 %; 591.46 ± 1.17 kcal /100 g DM. The resulting flour reached a pH of 6.5. The study of phytochemicals showed varying levels of oxalate (0.32 g/100g DM), tannins (3.02 g TAE/ 100g DM), total polyphenols (8.12 mg GAE / g DM), total flavonoids (27.5 mg QE/g DM) and carotenoids (2.9 µg/100g DM). Furthermore, they were potential sources of essential amino acids, including histidine (905.6 mg/100g), isoleucine (844.5 mg/100g), threonine (730.1mg/100g), phenylalanine (550.0 mg/100g), valine (469.9 mg/100g), leucine (456.6 mg/100g), and methionine (358.5 mg/100g).
 Conclusion: The overall result implies that Cucumeropsis mannii seeds could be added to the food preparations of malnourished children.

Acute and Sub-chronic Toxicity Evaluation of the Ethanolic Extract of Coula edulis B., (Olacaceae) Stem Bark

Aims: Coula edulis Baill., (Olacaceae) is a non-lignified forest product not well known and widely used in sub-Saharan Africa as a phytomedicine or food additive. However, the toxicity of this plant remains unknown. This study aimed to assess the safety of the ethanolic extract of C. edulis stem bark (CEE).
 Study Design: Pharmacological study.
 Place and Duration of Study: Laboratory of Nutrition and Nutritional Biochemistry, Department of Biochemistry, University of Yaounde 1 (Cameroon), between June 2018 and July 2022.
 Methodology: Studies on the assessment of acute and subchronic toxicity were carried out by guidelines 423 of the Organization for Economic Co-operation and Development (OECD). Subacute toxicity of the sample was assessed over 28 days using repeated doses by OECD Guideline 407.
 Results: No cases of death and clinical signs of toxicity were observed in the treated rats, suggesting that the LD50 of C. edulis ethanolic extract is greater than 2000 mg/kg bw. Regarding the subacute toxicity study, the administration of CEE also did not result in any changes in the course of body weight. Only a significant decrease in the relative weight of the ovaries in females at the highest dose of 600 mg/kg was observed. In males and females, CEE did not affect lipid profile markers or transaminase levels (AST, ALT). In addition, a small but non-significant (p> 0.05) increase in creatinine was observed without kidney dysfunction. In males, CEE induced an increase in mean corpuscular volume number at 600 mg/kg, while at the same time, mean corpuscular hemoglobin concentration decreased at the 300 mg/kg dose. In females, a significant increase in the number of monocytes, red blood cells, and hemoglobin level were observed. No difference in the levels of urea, glucose, and lipid markers was observed nor histological changes in the organs studied.
 Conclusion: As would be expected, exposure to CEE did not cause significant toxic effects in treated rats. Therefore, this plant extract can be safely recommended for therapeutic use.

Effect of Different Levels of Aflatoxin B1 in Concentrated Livestock Diets on Some Characteristics of Rumen Fluid in Laboratory with Sodium Bentonite

This study was conducted in the nutrition laboratory of the Department of Animal Production in the College of Agricultural Engineering Sciences at the University of Baghdad, Al-Jadriya. The experiment lasted for 90 days to investigate the effects of adding sodium bentonite (SB) to a concentrated diet contaminated with aflatoxin B1 on some characteristics of rumen fluid in a laboratory setting. Four diets were prepared, each contaminated with different concentrations of aflatoxin B1 (0, 20, 40, 60 ppb), and four different percentages of sodium bentonite (0, 3, 5, 7%) were added to each ration. The results showed a significant increase in pH values among the different treatments, with the diet contaminated with 40 ppb aflatoxin B1 (T3) recording the highest acidity compared to the control treatment at 0% concentration. However, there were no significant differences in pH rates between the treatments. The pH rates of 7% sodium bentonite addition did not differ significantly from 5% and 0%, while 3% had the lowest pH rate. Regarding volatile fatty acids (VFA), there was a significant increase in their percentage with the increase in sodium bentonite concentrations. The second treatment contaminated with 20 ppb aflatoxin B1 recorded the highest VFA percentage among the treatments, while the fourth treatment (T4) at 0% concentration recorded the lowest VFA percentage. Significant differences in VFA percentages were observed among all the treatments, with the highest VFA percentage in T2, followed by T3 and T1, while T4 recorded the lowest percentage. The analysis of ammonia nitrogen values showed a significant increase among the different treatments, with T2 recording the highest value among the treatments, and T4 at 0% concentration recording the lowest ammonia nitrogen value. Significant differences in the average percentage of ammonia nitrogen were observed among all the experimental treatments, with the highest value in T2, followed by T1 and T3, while T4 recorded the lowest percentage. The total number of microorganisms showed a significant increase with the increase in sodium bentonite concentrations, with T2 at 7% concentration having the highest number of microorganisms, while T4 at 0% concentration recorded the lowest number. Significant differences in the average total number of microorganisms were observed among all the experimental treatments, with T2 having the highest number, followed by T1 and T3, while T4 had the lowest number.

Effect of Adding Fermented Juice of Epiphytic Lactic Acid Bacteria Prepared with Different Sources and Levels of Soluble Carbohydrates on Chemical Composition, Fermentation and Quality Characteristics of Wheat Straw Silages

This study was conducted in Nutrition laboratory belonged to Animal Production Department-Agriculture College - Al-Qasim Green University to investigate the effect of adding fermented juice of epiphytic lactic acid bacteria (FJLB) prepared with different sources and levels of water soluble carbohydrates (WSC) on chemical composition, fermentation and quality characteristics of wheat straw silages (WSS). The FJLB was prepared from wild reed plant with three sources of WSC including glucose (G) at 1 and 2% (w/v, FJLB-G1, FJLB-G2), cane molasses (M) at 3 and 5% (w/v, FJLB-M3, FJLB-M5) and date molasses (D) at 3 and 5% (w/v, FJLB-D3, FJLB-D5). According to the source and level of FJLB, six samples of WSS were prepared by adding each FJLB at level of 1%. For control WWS, distilled water was added at similar quantity as FJLB (WWS-C). Urea and molasses were added to all samples at level of 1 and 10% on dry matter (DM) basis. Samples of WSS were tightly packed in double plastic bags and ensiled anaerobically for 45 days. Color of WSS samples were characterized with acceptable smell. Results showed that there was a significant decrease (p<0.01) in DM content of WWS samples prepared with FJLB-M3 and FJLB-M5 as compared with WWS-C. However, crude protein (CP), ether extract (EE) and crude fiber (CF) contents in WWS were not affected by addition of FJLB. Addition of FJLB improved fermentation characteristics of WWS, lactic acid (LA) and volatile fatty acids (VFA) were significantly (p<0.01) increased. Moreover, lower (p<0.01) DM losses were estimated in WSS prepared FJLBs.

HEMATOLOGY OF Osphronemus goramy FED CONTAINING FERMENTED TURI (Sesbania grandiflora) LEAF MEAL and CHALLENGED WITH Aeromonas hydrophila

Turi leaf (Sesbania grandiflora) is a natural ingredient with antibacterial compounds. This research was conducted from April to June 2022 at the Fish Nutrition Laboratory and the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine, Universitas Riau. This study aimed to obtain the best dose of fermented turi leaves in feed seen from hematology before and after the challenge test with A.hydrophila. The method used in this study is an experimental method using a one-factor Completely Randomised Design (CRD) with five treatment levels, namely Kn: (no treatment), Kp: (no cure and infected with A.hydrophila), P1: (fermented feed containing 15% turi leaves, 85% soy flour), P2: (30% turi leaves, 70% soy flour), and P3: (45% turi leaves, 55% soy flour). Fish were reared for 46 days. The challenge test was conducted on day 32 with A.hydrophila bacteria at a density of 108 CFU/mL, as much as 0.1 mL/head. The results showed that fermented feed contained 45% turi leaves and 55% soybeans (P3). Wheat flour obtained total erythrocytes 2.51x106 cells/mm3, hematocrit 40.33%, hemoglobin 10.40 g/dL, total leukocytes 10.13x104 cells/mm3, phagocytosis activity 21.13%, survival rate 90%, and absolute weight growth 9.52g. Water quality parameters were temperature ranging from 27-300C, pH 5.9-6.2, DO 5.5-5.9 mg/L, and NH3 0.015-0.017 m g/L. Based on the results of the study, it can be concluded that there is an effect of adding fermented turi leaves to feed on the hematology of gouramy before and after the A.hydrophila bacterial challenge test has a significant impact

Efektivitas Daun Kelor Terhadap Kadar Kadmium Dan LDL Kolesterol Dalam Darah Sebagai Indikator Penyempitan Pembuluh Darah (Aterosklerosis) Pada Tikus Putih Yang Terpapar Asap Rokok

Tobacco consumption in Indonesia is a health epidemic that has direct or indirect toxicity due to exposure of cigarette smoke which contains harmful substances, especially cadmium metal. Cadmium’s exposure can increase the risk of coronary heart disease due to increased LDL cholesterol. One of the cadmium toxicity treatment by utilizing Moringa oleifera leaves as a natural antioxidant considering the flavonoids, saponins, ascorbic acid, carotenoids, phenolics, interquinones as cadmium chelating agents and β-sitosterol content. Therefore, it was necessary to determine the effectiveness of Moringa leaves extract in lowering blood cadmium and LDL cholesterol levels as indicators of atherosclerosis in white rats exposed to cigarettes smoke. The type of research was an experiment with quantitative analysis, conducted at the nutrition laboratory of the Faculty of Public Health Airlangga University and the clinical chemistry laboratory of Surabaya Health Polytechnic in March-July 2023. The independent variable was the doses of Moringa leaf extracts, while the dependent variables were blood cadmium and LDL cholesterol levels of white rats. The analysis of blood cadmium levels used an atomic absorption spectrophotometer and used precipitation methods by a photometer for LDL cholesterol levels. Based on statistical analysis using one way anova showed that blood cadmium levels had a significant value of &lt; 0.05, followed by post hoc tests it was found that treatment group 2 had significant differences from normal groups. Moringa leaves extract dose of 1500 mg/kgbw has been effective in lowering cadmium and LDL cholesterol levels against the risk of atherosclerosis for 14 days. Keywords : Atomic Absorption Spectrophotometry, Cadmium, LDL Cholesterol, Moringa oleifera, photometer, Rattus norvegicus

Botanical Composition and Forage Quality as Swamp Buffalo (Bubalus bubalis) Feed In Jembrana Regency, Bali Province– Indonesia

This study aimed to study the botanical composition and feed quality for swamp buffalo in Jembrana Regency, province of Bali - Indonesia. Research using the survey method use samples obtained by proportional stratified sampling for the five districts in Jembrana Regency. Measuring the production and botanical composition of forage was carried out by using the square in the “Actual Weight Estimate” method. Chemical analysis of the feed carried out at the Laboratory of Nutrition and Animal Feed Science, Faculty of Animal Husbandry, University of Udayana. The results showed that the botanical composition of forage for swamp buffalo consisted of Brachiaria reptans, Panikum repen, Lercia Hexandra, Cynodon dactylon, Ischainum sp, Digitaria sp, Alysicarpus vaginalis, and weed. Only Alysicarpus vaginalis as leguminous plants have been detected. The crude protein content of the ration was quite high, namely 12.94% with a digestibility of 66.73%. Based on the results of the study, it can be concluded that the botanical composition of forage for swamp buffalo in Jembrana Regency is quite diverse with good quality. Leguminous plant introduction needs to be done to improve feed quality.

Chemical and antioxidant characteristics on rice crackers fortified with seaweed Eucheuma cottoni and dayak onion

This study aims to observe the addition of seaweed and Dayak onions to rice crackers on the nutritional content and antioxidant activity. This study has been carried out at the Nutrition Laboratory, University of Borneo Tarakan. The seaweed used is Eucheuma cottoni. Making crackers consists of several steps, namely material preparation, dough making, molding, and drying. The results given information that the addition of seaweed and Dayak onions had an effect on the nutritional content and antioxidant activity. Puli crackers fortified with seaweed and Dayak onions contain 8.04% protein, 1.07% fat, 1.77% water, 5.96% ash, and 83.16% carbohydrates. The antibiotic activity of seaweed and Dayak onion fortified Puli crackers using the DPPH method was greater than that of commercial Puli crackers, which was 4.07%.

Correlation of Serum Albumin Levels With Laboratory Parameters in Automated Peritoneal Dialysis and Continuous Ambulatory Peritoneal Dialysis Patients: A Prospective Cohort Study.

Background Peritoneal dialysis (PD) is a treatment option for end-stage renal disease (ESRD) patients, with automated peritoneal dialysis (APD) and continuous ambulatory peritoneal dialysis (CAPD) being the two main modalities. APD has reported benefits such as reduced peritonitis rates, improved ultrafiltration, and enhanced quality of life. However, some studies have found potential negative consequences of APD, and the impact on survival outcomes is limited and contradictory. Selecting the appropriate PD modality for ESRD patients should be individualized based on various factors, including nutritional status, demographic factors, laboratory findings, and other outcomes. PD patients are at high risk of malnutrition, and serum albumin is commonly used as a marker of nutritional status. Continuous monitoring of laboratory values may be beneficial for identifying nutritional deficiencies in a timely manner. Methodology This prospective cohort study aimed to compare APD and CAPD modalities in relation to serum albumin levels, demographic factors, and other laboratory parameters. The sample consisted of patients with ESRD treated with PD, who were divided into two groups per baseline albumin level. The study collected data on demographic, clinical, and laboratory characteristics, as well as comorbidities. The data were analyzed using SPSS version 26 (IBM Corp., Armonk, NY, USA), and statistical tests, such as the chi-square test and repeated-measures analysis of variance (ANOVA), were conducted to determine significant associations and differences between variables. Results The study included a total of 85 patients with ESRD who required PD as a treatment modality. Among them, 71 patients were undergoing APD, and 14 patients were undergoing CAPD. The study found that there were no significant differences in demographic factors, laboratory parameters, or medical history parameters between APD and CAPD patients with different albumin levels. The patients were followed up for six months and laboratory parameters were evaluated. Repeated-measures ANOVA showed that there were no significant variationsin both APD and CAPD patients. However, Spearman's rank correlation test revealed statistically important correlations between albumin and some laboratory parameters in both APD and CAPD patients at different assessment stages, including hemoglobin, sodium, transferrin, uric acid, phosphate, total protein, cholesterol, and triglycerides (p < 0.05). Conclusions Serum albumin levels appeared to be unaffected by the choice of PD modality. There were significant correlations between serum albumin levels and specific laboratory findings, including total protein, across all assessment stages for both APD and CAPD patients. These findings underscore the importance of continuous laboratory monitoring for PD patients.

Nutritional Laboratory Studies Prior to Total Knee Arthroplasty: Practice Versus Publication.

Recent emphasis has been placed on nutritional status assessment prior to total knee arthroplasty (TKA), including multiple American Academy of Orthopaedic Surgeons publications recommending specific laboratory studies; however, the frequency with which surgeons obtain these laboratory studies remains unclear. We sought to assess the incidence of ordering nutritional laboratory studies in the 90 days prior to TKA, utilizing data from a large administrative claims database. With use of the PearlDiver database, we identified 557,670 patients undergoing primary TKA from 2011 to 2020 with a metabolic panel or blood cell count claim within 90 days prior to TKA. We then determined the incidence of prealbumin, transferrin, vitamin D, and zinc laboratory tests claimed 90 days prior to TKA. Associations between claims and the year of surgery, patient demographics, and clinical characteristics were assessed by comparing proportions and chi-square testing. Nutritional laboratory studies were infrequently claimed within 90 days prior to TKA, with studies for prealbumin being performed in 2.2% of patients; transferrin, 1.9%; vitamin D, 10.2%; and zinc, 0.2%. From 2011 to 2020, there was a moderate but steady increase in the proportion of patients with claims for prealbumin (change from 0.8% in 2011 to 3.4% in 2020; p < 0.001), transferrin (0.8% to 2.7%; p < 0.001), and vitamin D (7.6% to 9.4%; p < 0.001) laboratory tests but there was less of a change for zinc (0.1% to 0.2%; p < 0.001). There were weak-to-absent associations of age, gender, obesity, diabetes, and anemia with laboratory claims. Despite multiple publications and recommendations, nutritional laboratory studies are infrequently ordered prior to TKA. Although there has been a slight increase in the use of nutritional laboratory studies over the past decade, patient factors such as gender and obesity were not associated with this increase. Understanding current practice patterns may help target future areas for improvement. Diagnostic Level III . See Instructions for Authors for a complete description of levels of evidence.

Hand Grip Strength: A Tool of Nutritional Assessment in Prevalent Hemodialysis Patients

Abstract Background Malnutrition is a common problem in patients with end stage renal disease (ESRD) undergoing hemodialysis that is associated with increased morbidity and mortality. There are several clinical, nutritional, and biochemical parameters that may be indicative of PEW in patients using hemodialysis (HD). Handgrip strength is a simple, quick, non-invasive, inexpensive, rapid, and objective procedure. In addition, it has other advantages as a diagnostic/screening tool for nutritional assessment. However, HGS relies on the motivation of the subjects, and therefore it is imperative that researchers and clinicians are sufficiently trained in HGS assessment. L-Carnitine supplementation, levocarnitine is an amino acid derivative which is an essential cofactor of fatty acid metabolism, may improve several situations. Aim of the Work The aim of our study, a longitudinal observational study, is to assess HGS as an independent tool for assessment of the nutritional status in hemodialysis patients. This study can also show the significance of the commitment to high protein diet (1.2-1.4 g/ kg/ d) and receiving L-carnitine (10-20 mg/kg intravenously after each dialysis session) and its effect on the handgrip strength and other studied nutritional tools. Patients and Methods In our study, a longitudinal observational study, study duration was 3 months after washout period; our population is all End Stage Renal Disease patients in Kasr AlAiny Hemodialysis Unit, Cairo University. The unit provides hemodialysis and medical service for 104 patients from whom 50 were excluded and 54 patients were enrolled. Results the study showed comparison between both studied groups regarding the studied laboratory parameters and different nutrition assessment tools showed no statistically significant differences. The study showed Comparison between the effect of high protein diet and L Carnitine (pre and post) in patients of group B on the studied nutritional tools and laboratory parameters. Again HGS was the only tool that showed statistical significance. %BF(body fat) assessed by anthropometry was negativelycorrelated with HGS. Also they found lower HGSvalues in patients on HD with higher estimates of abdominal fatdeposition. Thus, it seems that low muscle mass can occurdespite fat accumulation, and one possible explication for thiswould be the proinflammatory phenotype presented by individualswith abdominal fat deposition. This confirms that subjects can have normal or higher BMI and have a depletionprocess, thus increasing the need for screening proceduresrelated to functional status. Conclusion In this study, we concluded that HGS was the only significant (p value: &amp;lt;.001), while all other nutrition assessment tools didn’t show any significant difference.The correlation between nPCR and HGS was statistically significant (p value:.001, r:.616). L-carnitine supplementation has no role over commitment to high protein diet only regarding improving the nutritional status and hemoglobin level in hemodialysis patients.

Proximate Analysis of Unconventional Feed Available in Boalkhali Upazila, Chattogram

The study was conducted to evaluate the nutritional status of unconventional feed resources (UCFR) available in the Chattogram district. Unconventional feed resources refer to all those feeds that have not been traditionally used in another feeding and or are not normally used in commercially produced rations for livestock and poultry. A total of four tree leaves namely Jackfruit (Artocarpus heterophyllus), Mango (Mangifera indica), Banyan (Ficus benghalensis), and Mandar (Erythrina fusca) were considered for proximate analysis in the Animal Nutrition Laboratory, Department of Animal Science and Nutrition, Chattogram Veterinary and Animal Sciences University, Bangladesh. The laboratory analysis was performed according to the standard analytical procedure of the Association of Official Analytical Chemists (AOAC). The results indicated that the highest Dry mater (DM %) was in Jackfruits leaves whereas the lowest Dry matter (DM %) was in Mandar leaves. The highest Moisture % was in Mandar leaves whereas the lowest Moisture % was in Jackfruits leaves. The highest Crude protein (CP %) was in Mandar leaves whereas the lowest Crude protein (CP %) was in Mango leaves. The highest Crude fiber (CF %) was in Mandar leaves whereas the lowest Crude fiber (CF %) was in Banyan leaves. The highest Ether extract (EE%) was in Mango leaves whereas the lowest Ether extract (EE%) was in Jackfruit leaves and the highest Total Ash (TA%) was in Banyan leaves whereas the lowest Total Ash (TA%) was in Mandar leaves. In conclusion, the nutritional status of all tree leaves showed a higher Crude protein, Crude fiber, Ether extract, and Total Ash content. Therefore, these tree leaves can be used as a potential source of nutrients in livestock and poultry feed as well.

Pemanfaatan Silase Ransum Komplit Berbasis Ampas Tebu (Bagasse), Indigofera dan Molases sebagai Pakan Alternatif

Bagasse has not been utilized optimally because it has low nutrient content, it was necessary to add feed ingredients such as indigofera which has high nutrition and molasses was needed for further processing utilizing anaerobic fermentation or silage. This study aims to determine the nutritional quality of complete ration silage based on bagasse, indigofera and molasses with different compositions. Silage was carried out at the Laboratory of Nutrition and Feed Technology, Faculty of Agriculture and Animal Sciences, State Islamic University of Sultan Syarif Kasim Riau. The proximate test was carried out at the Laboratory of the Center for Biological Resources and Biotechnology Research, Agricultural University of Bogor. This study used a completely randomized design with a factorial pattern consisting of 2 factors, each treatment unit was repeated 3 times. Factor A was the level of bagasse + indigofera, namely A1 = 100% bagasse + 0% indigofera; A2 = 50% bagasse + 50% indigofera; A3 = 75% bagasse + 25% indigofera and factor B was the level of molasses, namely B0 = 5% molasses and B1 = 10% molasses. The variables measured were crude fat, ash content and BETN. The data obtained were analyzed based on analysis of variance by using Duncan's test at 5% and 1%. The results of this study indicated that there was an interaction between factor A and factor B (P&lt;0.01) on crude fat content, ash content, and BETN. Factor A had a very significant effect (P&lt;0.01) on the crude fat content, ash content, and BETN. Factor B had a very significant effect (P&lt;0.01) on crude fat and BETN, but did not affect the ash content. It was concluded that treatment A2, namely the composition of bagasse 50% + 50% indigofera and treatment B0, namely molasses 5%, obtained the best results.

Predictive Validity of Mortality after Surgically Treated Proximal Femur Fractures Based on Four Nutrition Scores-A Retrospective Data Analysis.

Hip fractures are becoming a growing concern due to an aging population. The high costs to the healthcare system and far-reaching consequences for those affected, including a loss of independence and increased mortality rates, make this issue important. Poor nutritional status is a common problem among geriatric patients and is associated with a worse prognosis. Nutritional screening tools can help identify high-risk patients and enable individualized care to improve survival rates. This retrospective study investigates four nutritional scores and laboratory parameters' predictive significance concerning postoperative mortality after surgical treatment of proximal femur fractures at 1, 3, 6, and 12 month/s for patients over 60 years using the chi-square test, Cox regression analysis, and receiver operating characteristics (ROC). The European Society for Clinical Nutrition and Metabolism (ESPEN) guidelines were used as part of the screening of the respective nutritional status of the patients, in particular to filter out malnutrition. A total of 1080 patients were included in this study, whereas 8.05% suffered from malnutrition, defined as a body mass index (BMI) below 18.5 kg/m2. The Mini Nutritional Assessment (MNA) screening tool identified the highest proportion of malnourished patients at 14.54%. A total of 36.39% of patients were at risk of malnutrition according to three nutrition scores, with MNA providing the most significant proportion at 41.20%. Patients identified as malnourished had a higher mortality rate, and MNA screening was the only tool to show a significant correlation with postoperative mortality in all survey intervals. The MNA presented the best predictive significance among the screening tools, with a maximum area under the curve (AUC) of 0.7 at 12 month postoperatively. MNA screening has a solid correlation and predictive significance regarding postoperative mortality-therefore routine implementation of this screening in orthopedic/traumatology wards is recommended. Moreover, nutritional substitution therapy can offer a relatively inexpensive and easy-to-implement measure. The Graz malnutrition screening (GMS) shows moderate predictive power and could be considered as an alternative for patients under 60 years of age. A higher albumin level is associated with improved survival probability, but cannot be indicative of nutritional status.

Monitoring the effect of perioperative nutritional care on body composition and functional status in patients with carcinoma of gastrointestinal and hepatobiliary system and pancreas.

The significance of nutritional care in the management of cancer, particularly in the surgical treatment of abdominal cancer, is increasingly acknowledged. Body composition analysis, such as the Bioelectric impedance assay (BIA), and functional tests, e.g., handgrip strength, are used when assessing nutritional status alongside general and nutritional history, clinical examination, and laboratory tests. The primary approach in nutritional care is individually adjusted nutritional counselling and the use of medical nutrition, especially oral nutritional supplements. The aim of the study was to investigate the effects of perioperative nutritional care on body composition and functional status in patients with carcinoma of the gastrointestinal tract, hepatobiliary system, and pancreas. 47 patients were included, 27 received preoperative and postoperative nutritional counselling and oral nutritional supplements (Group 1), while 20, due to surgical or organisational reasons, received nutritional care only postoperatively (Group 2). The effect of nutritional therapy was measured with bioimpedance body composition and handgrip measurements. Group 2 had a higher average Nutritional Risk Screening (NRS) 2002 score upon enrolment (3 vs. 2 points); however, there was no difference when malnutrition was assessed using Global Leadership in Malnutrition (GLIM) criteria. There was a relative increase in lean body mass and fat-free mass index (FFMI) 7 days after surgery in group 1 (+4,2% vs. -2,1% in group 2). There was no difference in handgrip strength. Our results indicate that combined preoperative and postoperative nutritional care is superior to only postoperative nutritional care. It seems to prevent statistically significant lean mass loss 7 days after surgery but not after 14 days or 4 weeks.

Manifestaciones dermatológicas en una lactante con síndrome de kwashiorkor: Claves para el diagnóstico y tratamiento oportuno

Kwashiorkor syndrome is characterized by protein malnutrition and edema, risk factors are recent weaning, infections, and diets based on corn and rice. This malnutrition can lead to skin manifestations such as thin, dry skin, hyperpigmentation, confluent areas of scaling, dry, hypopigmented, and detachable hair, as well as erosive dermatitis, predominantly in skin folds. The diagnosis is based on a nutritional evaluation exam, physical examination and laboratory finding, the treatment is based on early nutritional rehabilitation. Clinical case: 8-month-old female infant who attended the Instituto Nacional de Pediatría, presenting a scaling dermatosis of 1 months of evolution that was treated with topical ketoconazole. The mother reported exclusive feeding with corn gruel due to the diagnosis of “lactose allergy” and commented not enough resources to buy hydrolyzed formula. The patient presented a disseminated dermatosis to all- body segments, characterized by well-defined, irregularly shaped, hyperpigmented plaques with scaling in thick sheets in the perioral region and extremities, as well as areas of eroded skin and paleness and edema of extremities. Laboratory tests were taken, where anemia (Hb 11.2 g/dl) and hypoalbuminemia (3.3 g/dl) were documented, the diagnosis of Kwashiorkor syndrome was integrated. The clinical and laboratory findings were reported to the Gastroenterology and Nutrition service, who performed a nutritional assessment, and began nutritional treatment, emollients and general skin care were documented; twenty days later, the dermatosis and edema had subsided. Keywords: Malnutrition; dermatoses; edema; feeding; desquamation.