Laboratory Facilities Research Articles

B-023 Workflow evaluation of the new Siemens Atellica CI Analyzer

Abstract Background The Atellica® CI Analyzer, Model 1900 is an integrated clinical chemistry and immunoassay analyzer that has the same user interface, general workflow, and planned test menu to that of the larger Atellica® Solution. Atellica CI Analyzer also shares the same reagents and consumables as the Atellica Solution. The purpose of this study was to evaluate workflow performance of the Atellica CI Analyzer in a routine-like clinical laboratory setting. Methods The study was conducted at four international laboratory facilities over a four-month period. Basic throughput of the Atellica CI Analyzer was evaluated at each site by testing quality control (QC) and remnant anonymized patient samples using an 8:2 mixture of chemistry (ion selective electrolytes [IMT], clinical chemistry analytes) and immunochemistry assays. Additionally, the impact of interrupting routine testing for STAT high-sensitivity cardiac troponin samples was also evaluated. Daily, weekly, and monthly maintenance activities were observed, and data collected through direct observations, time and motion studies, and system run logs. Results The Atellica CI Analyzer aspirated patient samples in as little as two minutes (mins) with the time to first result for the mixed assay worklist ranging from 1 to 12 mins from the time of first barcode read. Across the four sites, the time to last result for the mixed assay worklist (average n=495 test results per site) ranged from 52 to 65 mins. The average turn around time (TAT) for high-sensitivity troponin was 9 mins from barcode read. Daily maintenance and QC activities for the Atellica CI Analyzer are comprised of both automated and manual activities. The average daily maintenance hands-on time was 3.6 min, ranging from 1.6 to 6.4 mins across the four sites. Daily QC preparation required <5 mins of hands-on time. The total average hands-on time to complete weekly maintenance for the Atellica CI Analyzer was 7.5 min. Conclusions The Atellica CI Analyzer demonstrated consistent system performance across all four study sites. Implementation of the Atellica CI Analyzer has the potential to increase laboratory efficiency given the minimal hands-on time required for daily and weekly maintenance activities and short TATs.

Development of Project Based Learning Based Practicum Modules to Improve High School Students Understanding of Concepts

Abstract The aim of physics learning in high school is for students to physics concepts and their relationships and a scientific attitude to solve the problems they. Based on issues in independent curriculum and condition of SHS Country 8 ST being a newly established school still in the process of building its school premises, hence the laboratory facilities are not adequate, there is a need for the development of practicum modules for physics learning. Model in this research uses Analysis, Design, Development, Implementation, and Evaluation (ADDIE) model. Results of the trial on grade XII MIPA students obtained satisfactory results for use. By using a questionnaire several questions about understanding physics concepts for this research. The limited trial results obtained an average score of 3.9 or 78% the category good, and based on the expanded trial results, an average score of 4.04 or 80.08% the category good was obtained. In conclusion “the Project Based Learning physics laboratory module dynamic electricity materials” is suitable for learning use in conditions of new and inadequate school infrastructure for carrying out practicum activities with PHET Simulation.

A Comprehensive Review of the Occurrence, Distribution, Characteristics and Fate of Per- and Polyfluoroalkyl Substances in the African Continent

AbstractPer- and polyfluoroalkyl substances (PFAS) represent synthetic organic compounds extensively utilized in a variety of domestic and industrial products due to their distinctive characteristics. Designated as persistent organic pollutants (POPs) by the Stockholm Convention, PFAS are recognized for their long-lasting presence, widespread prevalence, and adverse impacts on both the environment and human health. Recently, the United States Environmental Protection Agency (US EPA) revised the guideline limits for perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) in drinking water, setting them at 0.02 and 0.004 ng/L, respectively. Despite the global emphasis on the necessity for monitoring and treating PFAS in environmental media, there is a scarcity of information on PFAS studies in Africa. This gap may be attributed to a lack of modern laboratory facilities, weak governance, and the enforcement of environmental regulations. This study comprehensively reviews PFAS, focusing on key areas such as global guidelines and regulations, sources and distribution in the environmental matrix in Africa, characteristics, environmental fate, reported methods for sampling and analysis in Africa, and the role of government in the National Implementation Plan (NIP) on the continent. Additionally, the study offers recommendations and identifies knowledge gaps. In Africa, PFAS have been detected in various environmental compartments, including drinking water ranging from 0.03 to 200 ng/L, surface water ranging from 0.0254 to 788 ng/L, sediment ranging from 0.50 to 248.14 ng/g, wastewater ranging from 0.9 to 507 ± 257.6 ng/L, seafood ranging from 0.12 to 179.2 ng/g, sludge ranging from 0.01 to 0.098 ng/g, plants ranging from 0.160 to 29.33 ng/g, and indoor dust ranging from 1.3 to 69 ng/g. Notably, perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) are prevalent in samples from various environmental matrices across Africa. Reported sources of PFAS in African countries include municipal waste, hospital waste from medical equipment, discharge from industries, and wastewater treatment plants. Urgent attention is required from decision-makers and researchers in Africa to address PFAS monitoring, regulation, and treatment within the continent. Graphical abstract

Assessing Implementation of Strengthening Mathematics and Science in Secondary Education (SMASSE): In the Case of Selected Secondary School of Kellem Wollega Zone, Oromia Regional State, Ethiopia

This study aimed to identify the methods teachers use to integrate science and mathematics. These factors affect the implementation of integrated science and mathematics education and students' attitudes toward this integration in secondary schools in the Kellem Wollega zone of Ethiopia. Both open-ended and closed-ended questionnaires, along with structured interviews, were designed for the selected sample population. This targeted population was chosen from five different sites within the zone, comprising a total of 506 samples, of which 392 were students and 114 were teachers and school principals. The study found that teachers integrate science and mathematics using the PDSI approach, the ASEI approach, a student-centered approach, and generally the SMASSE approach. They employ various strategies, including assigning projects, conducting practical work with students, providing assignments and worksheets, and fostering team spirit among students. Several factors hindering the implementation of SMASSE in the study area were identified, including the teacher-student ratio, the disproportion between class size and the number of students, students' attitudes toward science and mathematics, a lack of laboratory facilities and equipment, an absence of a planned schedule, insufficient access to computers, inadequate assessment practices, a lack of sufficient time to complete the curriculum, and an inadequate quality assurance system. On the other hand, students exhibited a negative attitude toward the integration of science and mathematics education, which was influenced by factors such as their perceptions of mathematics, student-teacher interactions, feedback from teachers, teaching methods, course content, teachers' competencies, school management, and limited student participation in the classroom.

Effects of Hands-on Laboratory Experiment on Students’ Skills Achievement in Physics in Day Secondary Schools in Rwanda: A Case of Gicumbi District

This research investigated the effect of a hands-on laboratory experiment on students’ skills achievement in physics in day secondary schools in Gicumbi district, Rwanda. Specifically, this research examined the hands-on laboratory practices that affect students’ skills and achievement in physics in day secondary schools in Rwanda, analyze the level of students’ skills achievement in physics that is due to the hands-on laboratory experiment in day secondary schools in Rwanda, and establish the relationship between the hands-on laboratory experiment and students’ skills achievement in physics in day secondary schools in Rwanda. The study adopted a descriptive survey design with a mixed approach. The targeted population of the study consisted of 72 headteachers, 5416 students, and 1750 teachers, making the total population of 7238, selected across Gicumbi district. The researcher used purposive sampling techniques to choose physics teachers and students and simple random sampling to choose schools and headteachers, a descriptive and correlational research design was used in combination with a mixed methodology. Quantitative data were collected using questionnaires, while qualitative data were acquired using interviews. The findings were analyzed through descriptive statistics such as frequency, percentage, mean, and standard deviation, as well as inferential statistics such as correlation and regression analysis. The findings revealed that 72.2% of students felt confident in applying theoretical knowledge to practical experiments, showcasing the effectiveness of structured hands-on practices. However, 67.6% expressed dissatisfaction with the clarity of laboratory activities, indicating a need for improvement. Additionally, 78.1% of students reported increased confidence in performing physics calculations after lab experiments, while 60% felt their understanding of complex concepts could be enhanced through hands-on activities. Furthermore, 75% of students believed practical experiments helped them retain physics concepts better than theory alone, and 87% of teachers agreed that regular exposure to laboratory activities boosts student performance in assessments. Collectively, these findings highlight the need to enhance hands-on laboratory practices to improve student engagement and achievement in physics. The research recommends curriculum developers to create structured hands-on activities aligned with theoretical concepts and incorporate diverse instructional strategies. NESA needs to integrate practical assessments into evaluations, while the Ministry of Education should allocate resources for improving laboratory facilities and provide professional development for teachers.

A rapid tick exposure test for monitoring acaricide resistance in Rhipicephalus sanguineus sensu lato ticks on dogs

BackgroundBrown dog ticks (Rhipicephalus sanguineus sensu lato) are vectors of pathogens adversely affecting the health of dogs in many regions of the world. The three-host life cycle of R. sanguineus s.l., with all stages feeding on dogs, can lead to an uncontrolled build-up of large tick populations if not controlled by acaricides. However, frequent tick control on dogs using acaricides has led to the emergence of resistance to permethrin and fipronil. Currently, the larval packet test (LPT) is the standard tick resistance test, which is laborious, requires laboratory facilities, and takes at least 6 weeks before larvae derived from engorged female ticks can be tested. Our novel approach is to expose semi-engorged adult ticks to acaricides immediately after removing them from dogs, obtaining results within 24 h.MethodsAdult ticks from three laboratory colonies of R. sanguineus s.l. were tested in RaTexT®, a rapid tick exposure test in which ticks were confined to small compartments and exposed to an acaricide-impregnated, specially designed matrix. Resistance was confirmed by testing larvae derived from the same laboratory colonies using the LPT. RaTexT® was also used to determine the susceptibility of R. sanguineus acaricides in dog shelters.ResultsRaTexT® detected resistance to permethrin in adult R. sanguineus s.l. ticks from two Brazilian laboratory colonies compared to a susceptible laboratory strain originating in Greece. Resistance was confirmed by LPT testing of larvae from the same colonies with resistance factors between 2.2 and 3.1. All laboratory strains were susceptible to fipronil. A suspected case of fipronil resistance at a dog shelter in Caxias do Sul, Brazil, was resolved within 24 h by testing adult ticks in RaTexT® and could be attributed to improper treatment.ConclusionsRaTexT® is a valuable tool for monitoring the development of resistance to synthetic pyrethroids or phenylpyrazoles in tick-infested dogs.Graphical

Precise mass measurements of A = 133 isobars with the Canadian Penning Trap: Resolving the [formula omitted] anomaly at 133Te

We report precision mass measurements of 133Sb, 133g,mTe, and 133g,mI, produced at CARIBU at Argonne National Laboratory's ATLAS facility and measured using the Canadian Penning Trap mass spectrometer. These masses clarify an anomaly in the 133Te β-decay. The masses reported in the 2020 Atomic Mass Evaluation (M. Wang et al., 2021) produce Qβ−(133Te)=2920(6) keV; however, the highest-lying 133I level populated in this decay is observed at Ei=2935.83(15) keV, resulting in an anomalous Qβ−i=−16(6) keV. Our new measurements give Qβ−(Te133)=2934.8(11) keV, a factor of five more precise, yielding Qβi=−1.0(12) keV, a 3σ shift from the previous results. This resolves this anomaly, but indicates further anomalies in our understanding of the structure of this isotope.

Fill, Fold, Photo: Preconcentration and Multiplex Detection of Trace Level Heavy Metals in Water.

Heavy metal contamination is an increasing global threat to human and environmental health, particularly in resource-limited areas. Traditional platforms for heavy metal detection are labor intensive and expensive and require lab facilities. While paper-based colorimetric sensors offer a simpler approach, their sensitivity limitations prevent them from meeting legislative requirements for many metals. Existing preconcentration systems, on the other hand, can achieve lower detection limits but typically focus on analyzing only one metal, making comprehensive monitoring difficult. We address these limitations by introducing a low-cost preconcentration system coupled with colorimetric analysis for the simultaneous detection of seven metal ions at low ppb levels without the need for external equipment outside a smartphone. The system achieved detection limits of 15 ppb (Ni(II)), 7 ppb (Cu(II)), 2 ppb (Fe(III)), 20 ppb (Cr(VI)), 13 ppb (Pb(II)), 26 ppb (Hg(II)), and 15 ppb (Mn(II)) with six out of seven limits of detection values falling well below EPA regulatory guidelines for drinking water. The user-friendly Fill, Fold, Photo approach eliminates complex pretreatment steps. Smartphone-based detection offers portable quantification within seconds. Employing masking strategies ensured higher selectivity for each assay on the card, while our packaging protocols enable system stability for over 4 weeks of study, facilitating mass production and deployment within a realistic time frame. To validate the sensor's performance in real-world scenarios, the sensor was tested with environmental water samples. The sensor demonstrated good recovery, ranging from 77% to 94% compared to the standard ICP-MS method. Furthermore, spike recovery analysis confirmed the sensor's accuracy, with a relative standard deviation (RSD) of less than 15%. This technology holds significant promise for future development as a convenient, portable solution for field-based monitoring of a broad spectrum of water contaminants, including pesticides, PFAS, fertilizers, and beyond.

Longline and mesocosm cultivation of serrated wrack Fucus serratus (Phaeophyceae)

ABSTRACT Seaweed aquaculture is becoming increasingly important as the global seaweed market is set to exceed £20 billion by 2030, leaving natural populations at risk of overexploitation. Fucoids, such as Fucus serratus, are intertidal seaweeds hand-harvested for their use in agricultural, pharmaceutical, and biomedical industries. Despite many aspects of the biology of fucoids being well researched, there have been very few studies on their aquaculture. In this study, we grew F. serratus biomass from gamete life stages in the Queen’s University Marine Laboratory for 8 months and monitored their growth, amino acid and phenolic contents in a subtidal field trial. Gametes of F. serratus were successfully fertilized in vitro and attached to substrata: Tiles (mesocosm) and Dyneema Twine (subtidal). The growth of F. serratus on each substrate was monitored for 6 months in the laboratory facility with juveniles reaching 2–4 mm in length. The juveniles on twine were then deployed at the Strangford Lough test site and growth was compared against the juveniles on tiles after an additional two months. Prior to field deployment, individuals grown on tiles were significantly longer (~17%) than those on twine growing to ~3 cm, however, after the two-month deployment, the twine thalli grew significantly longer than those on the tiles (~33%) reaching ~6 cm in length. Subtidal F. serratus grown on twine had significantly higher amino acid content, whereas the phenolic content was significantly lower (~62 mg PGE g−1) than seaweed grown on the tiles (~128 mgPGE g−1). Growing F. serratus successfully in subtidal areas is highly important for providing sustainably sourced biomass to reduce reliance on wild harvesting. Further, cultivating in the subtidal zone will help to avoid competition for space from other aquaculture industries operating in the intertidal zone.

Combination of control factors influencing application uniformity of a low-pressure pivot sprinkler spray unit

The objective of any pivot irrigation system is to ensure uniformity of application of water which can be achieved when responsible factors such as pressure, drop tube lengths, nozzle size etc. are adequately designed. This indoor experiment conducted in a 44m2 laboratory facility tends to evaluate the extent of the influence of some sprinkler design parameters on the performance of a low pressure single pivot sprinkler spay unit. The parameters considered are; pressures of 9,12, 15 and 19Psi, nozzle sizes of 4.17, 5.56, 6.95 and 8.14 mm and drop tube lengths of 1, 1.2 and 1.5m. In this experiment an inverted U-shaped frame designed to support a spray sprinkler at different heights was used. The hydraulic installation with manual throttle valves for controlling water distribution was used to supply pressurized water to the spray model sprinkler. A number of 64 graduated catch-cans with 0.16 m diameter and 0.2m height were used. The cans were arranged in eight radial legs with a distance of 1m apart. The system was run for one hour then the caught volumes were measured manually using a graduated cylinder with a capacity of 500 mL. The catch-cans data were used to determine uniformity coefficients. Line graphs were used to show relationships between the parameters measured. The experiment shows that there is strong relationship between the parameters considered. It indicated that the coefficient of uniformity (CU) and distribution uniformity of lower quarter DUlq increases with increase in nozzle size and pressure. It also shows that the largest nozzle size and pressure within the limits of this experiment gave the best CU and (DUlq) values of 96% and 90% at 1.5m height. Lager nozzle sizes with lower pressure reduces the CU and DUlq values. It is concluded from this experiment that though pressure, nozzle size and height of application influences the uniformity of application, larger nozzle sizes accompanied by higher pressure and height of application resulted in good uniformity of application. It is therefore imperative that while operating Pivot Sprinkler systems, careful selection of nozzle diameters, operating pressure and drop tube heights, sprinkler can be used to apply the ideal amount of irrigation water needed to refill the crop root zone that can neither cause runoff nor harm the crop and also provide the best uniformity possible under the prevailing wind and management conditions.

Evaluation of an Enzyme-Linked Magnetic Electrochemical Assay for Hepatitis a Virus Detection in Drinking and Vegetable Processing Water

Globally, waterborne viral infections significantly threaten public health. While current European Union regulations stipulate that drinking water must be devoid of harmful pathogens, they do not specifically address the presence of enteric viruses in water used for irrigation or food production. Traditional virus detection methods rely on molecular biology assays, requiring specialized personnel and laboratory facilities. Here, we describe an electrochemical sandwich enzyme-linked immunomagnetic assay (ELIME) for the detection of the hepatitis A virus (HAV) in water matrices. This method employed screen-printed electrodes as the sensing platform and utilized commercially available pre-activated magnetic beads to provide a robust foundation for the immunological reaction. The ELIME assay demonstrated exceptional analytical performance in only 185 min achieving a detection limit of 0.5 genomic copies per milliliter (g.c./mL) and exhibiting good reproducibility with a relative standard deviation (RSD) of 7% in HAV-spiked drinking and processing water samples. Compared with the real-time RT-qPCR method described in ISO 15216-1, the ELIME assay demonstrated higher sensitivity, although the overall linearity of the method was moderate. These analytical attributes highlight the potential of the ELIME assay as a rapid and viable alternative for HAV detection in water used for agriculture and food processing.

Out-of-Pocket Expenditures for Health: A Case Study

Background of the Study: Health is a prime component in rural population who uses their muscle power for doing economic activities. Meanwhile according to the Economic Survey of India’s 2021 report, India was among the 10 worst performing countries in terms of prioritising health in government budgets, at both state and central levels. India was placed or ranked in 67th in the list of 189 countries in Out of Pocket Expenditure per capita in PPP (in $) for 2018. World Health Organisation report (March 2022) stated that high of Out-Of-Pocket Expenditure (OOPE) on health is impoverishing some 55 million Indians annually, with over 17 per cent households incurring catastrophic levels of health expenditures every year. The Economic Survey 2023 reported that the share of government expenditure in Total Health Expenditure (THE) has increased over time from 28.6% in financial year 2014 to 40.6% in Financial Year2019. However, OOPE is still higher at 48.2% of the total expenditure. Objectives: To explore the Out of Pocket Expenditure (OOPE) for health in India, Interstate and rural villages of Theni district, Tamil Nadu Methodology: Purposive non random sampling was used to reach the sample element. Sample elements or respondents were from rural Theni District. Sample size was 300. Data was collected using an interview schedule in the catchment area of Primary Health Centre, taluk hospital and district healthcare hospital. Interview was conducted in Tamil and then the interview output was transcribed and translated into English, followed by a descriptive analysis. Ordinary Least Square estimation was used to find the implications of individual income on pocket out expenditure for health. Findings of the Study: The secondary data from the website of World Health Organisation and from the reports of National Health Accounts reflects a difference in data information for every concerned period at least two to three per cent exist. The interstate data information for the period 2019 to 2020 revealed that the spending from government health care expenditure and out of pocket expenditure is not equal to total health care expenditure. Hence, the researcher made an attempt to explore the out of pocket expenditure among the rural population and their willingness to spend from their pocket. Furthermore, the impact of annual income and annual healthcare expenditure is examined by simple linear regression analysis, for to understand the role of income in shaping healthcare spending behaviour. The R Square value is very poor, and reflects the role of explanatory variable income is not impacting the out of pocket health care expenditure. The Keynesian theory of psychological law of consumption is invalid in out of pocket healthcare expenditure with respect to income of the respondents. 13 percentages of respondents were willing to do out of pocket expenditure for their health ailing. The 39.7percentages of respondents were disagreeing to do out of pocket expenditure for their health. Conclusion: Overall, this study emphasizes the importance of understanding out-of-pocket health expenditures is increasing in rural (feckless management of public healthcare) with attitude of unwilling to pay from their pocket because of existing zero priced healthcare system, which is available in their near locality. Policy makers needs to concentrate on enhancing the healthcare supply such as medical personals, drugs, lab facility, beds, and in general in all aspects for to cater the demand of rural inhabitants and protect them from pushing into impoverishment.

Lower Microscopy Sensitivity with Decreasing Malaria Prevalence in the Urban Amazon Region, Brazil, 2018-2021.

Malaria is increasingly diagnosed in urban centers across the Amazon Basin. In this study, we combined repeated prevalence surveys over a 4-year period of a household-based random sample of 2,774 persons with parasite genotyping to investigate the epidemiology of malaria in Mâncio Lima, the main urban transmission hotspot in Amazonian Brazil. We found that most malarial infections were asymptomatic and undetected by point-of-care microscopy. Our findings indicate that as malaria transmission decreases, the detection threshold of microscopy rises, resulting in more missed infections despite similar parasite densities estimated by molecular methods. We identified genetically highly diverse populations of Plasmodium vivax and P. falciparum in the region; occasional shared lineages between urban and rural residents suggest cross-boundary propagation. The prevalence of low-density and asymptomatic infections poses a significant challenge for routine surveillance and the effectiveness of malaria control and elimination strategies in urbanized areas with readily accessible laboratory facilities.

Use of Science Laboratory Facilities and Students Academic Performance in Chemistry Subject in Rwanda, A Case of Kayonza Public Secondary Schools

Use of Science Laboratory Facilities and Students Academic Performance in Chemistry Subject in Rwanda, A Case of Kayonza Public Secondary Schools

Scurvy with Musculoskeletal Pain in Indonesian Pediatric Patient: A Case Report

Background: Scurvy is a potentially fatal disease caused by a severe deficiency of vitamin C, which can manifest as a musculoskeletal problem. Early diagnosis and treatment of vitamin C deficiency are crucial to prevent severe symptoms. Unfortunately, vitamin C level evaluations in Indonesia are very limited due to the lack of laboratory facilities. As a result, patients with scurvy are often underdiagnosed and do not receive adequate management. Case Presentation: 5-year-old boy with musculoskeletal pain and inability to walk. Radiographic findings showed a pathognomonic line of Fränkel, Trümmerfeld zone, and Wimberger ring sign, with laboratory evaluation showing an anemia and vitamin D deficiency. We did not perform a laboratory examination for vitamin C due to a lack of test facilities but the patient was given oral 250 mg of vitamin C daily instead. The patient showed significant improvement in symptoms and laboratory results after three months of vitamin C supplementation. To summarize, that vitamin C deficiency should be considered among patients with related musculoskeletal manifestations supported by imaging results.

RaTexT®: a novel rapid tick exposure test for detecting acaricide resistance in Rhipicephalus microplus ticks in Brazil

BackgroundAcaricide resistance in cattle ticks is a significant concern in (sub)tropical regions, particularly Brazil. The Larval Packet Test (LPT) is the standard laboratory bioassay for resistance diagnosis, which requires triplicates of seven acaricidal dilutions plus controls to cover larval mortalities ranging between 0 and 100%. The value of the LPT lies in providing resistance ratios based on the ratio between the LC50 calculated with potentially resistant and susceptible ticks. However, LC50 ratios are difficult to translate into practical advice for farmers. Moreover, LPT requires laboratory facilities to maintain susceptible tick colonies, and it takes 6 weeks to obtain the larvae to be tested by LPT derived from engorged female ticks collected from cattle in the field. Our novel approach was twofold: first, we upgraded the LPT to the Resistance Intensity Test (RIT) by adopting the latest WHO guidelines for resistance detection in mosquitoes, which combines a 1 × recommended dose with 5 × and 10 × concentrated doses to reveal low, moderate and high resistance intensity, respectively. This reduced the number of test papers and tick larvae and, more importantly, provided relevant information on the resistance level. Our second innovative step was to abolish testing larvae entirely and expose partly engorged adult ticks to the same acaricidal doses immediately after removing them from cattle in the field. This resulted in the Rapid Tick exposure Test (RaTexT®), wherein partly engorged adult ticks were exposed to an acaricide-impregnated, specially designed matrix providing test results within 24 h. This approach directly compared resistance detection in tick larvae in the RIT with resistance in adult ticks in RaTexT®.MethodsLaboratory validation was conducted in Brazil with resistant and susceptible colonies of Rhipicephalus microplus ticks. For field validation, adult R. microplus ticks collected from different cattle farms in Brazil were evaluated for resistance to RaTexT®, and the results regarding their larval progenies were compared with those for the RIT. Partly engorged adult ticks derived from cattle infested with laboratory and field strains of R. microplus were exposed to deltamethrin in RaTexT® containers, which contained six rows of four interconnected compartments, accommodating five to eight semi-engorged female ticks with a preferred size ranging between 5 and 8 mm. The corresponding larvae of each strain were exposed in the RIT to the same deltamethrin concentrations in filter papers.ResultsIn RaTexT®, mortality in adult ticks from a resistant strain of R. microplus from Seropédica in Brazil was 38.4%, 54.2% and 75.0% at the 1 ×, 5 × and 10 × doses of deltamethrin, respectively. In RIT, mortality of larvae from the same resistant strain was 2.0%, 4.9% and 19.5% at 1 ×, 5 × and 10 × doses, respectively. The results of RaTexT® and RIT agreed since both tests identified a high level of resistance based on a cut-off of 90% mortality.In RaTexT®, mortality of adult ticks from a susceptible strain originating from Porto Alegre was 73.8%, 92.9% and 97.6% at the 1 ×, 5 × and 10 × doses, respectively. In RIT, mortality of larvae from the susceptible strain was 95.2%, 95.2% and 96.8% at the 1 ×, 5 × and 10 × doses, respectively. Interestingly, both tests identified a low number of unexpected resistant individuals in the susceptible strain since the mortality of neither larvae nor adults reached 100%. This effect remained unnoticed in the LPT, wherein a resistance ratio of 159.5 was found based on the LC50 of the resistant strain divided by the LC50 of the susceptible strain. Next, RaTexT® was compared with RIT using adult and larval ticks derived from three field strains of R. microplus in Brazil. RaTexT® detected high levels of resistance to deltamethrin in adult ticks in all strains, which was confirmed in larvae tested by the RIT. Both tests agreed on the same resistance level with significantly lower mortality rates in larvae than in adult ticks.ConclusionsRaTexT® is a novel rapid pen-site test for detecting acaricide resistance in adult livestock ticks. It potentially replaces laborious tests using larval ticks and provides results within 24 h relevant to acaricide resistance management of livestock ticks.Graphical

The Digital Lab Facility Manager: Automating operations of research laboratories through “The World Avatar”

This paper presents a novel framework for digitalizing and automating the management of specialized research laboratories using The World Avatar, a general all-encompassing dynamic knowledge graph. Semantic Web technology empowers users to effectively utilize data across various systems and formats without restricting them to a single software or protocol. Employing agents and ontologies, it enables seamless data sharing, computational reasoning, and gradual automation of tasks, addressing obstacles in interoperability and adaptability. Showcasing the capabilities of this approach, we tackle some common challenges in lab facility management, including cost-effective Internet of Things (IoT) sensor network integration with an existing building management system and efficient airflow management for fume hoods via “human-in-the-loop” interventions. We also develop unified platform-agnostic interfaces that complement the framework. These advancements represent a significant stride in the holistic digitalization and automation of research laboratories at the nexus of fundamental science and smart building applications, setting a foundation for future research facilities to achieve operational excellence and sustainability.

Exploring the Life and Academic Experiences of International Students at the University of Jambi, Indonesia: A Case Study

The increasing number of international students in Indonesia's higher education sector urges institutions to improve the quality of their services, facilities, and education. The teaching and learning environment is critical, encompassing the effectiveness of instructional methods, classroom interactions, and the academic support systems provided by the university. Additionally, their socio-experiences, including integration into the campus community and interactions with peers, play a crucial role in their overall well-being and academic success. This study aims to explore the life and academic experiences of international students in undergraduate International Programs at the University of Jambi in Indonesia. This research employed a mixed-method approach, combining a questionnaire adapted from the Strategy Inventory for Language Learning (SILL) and in-depth interviews, with a total of 13 international students from international classrooms participating in the study. The research highlights significant challenges for international students at the University of Jambi, including inadequate classroom and laboratory facilities, poor lighting, and insufficient resources, particularly in Animal Sciences and Agriculture. Language barriers due to Indonesian being the primary medium of instruction further hinder learning. Students express high dissatisfaction with services such as insurance, health facilities, extracurricular activities, and counseling, though library services are well-regarded. Recommendations include adopting English for instruction, providing bilingual training for lecturers, and improving infrastructure and student services to enhance the overall academic experience for international students

On the model flexibility of the geographical distributed real-time co-simulation: The example of ENET-RT lab

The decarbonization of the energy sector represents a challenge that requires new tools and approaches of analysis. This paper aims to demonstrate the fundamental role that geographical distributed real-time co-simulations (GD-RTDS) can play in this regard. To this end, three different case studies have been analyzed with GD-RTDS, covering a wide range of applications for the energy sector decarbonization: (a) implementation of Renewable Energy Communities for supporting the share increase of Renewable Energy Sources, (b) the integration and management of Onshore Power Supply, and (c) the integration of a forecasting tool for the management of the Electric Vehicle charging. The performed experiments included fully simulated components, together with (power) hardware-in-the-loop and software-in-the-loop elements. These components have been simulated in different laboratory facilities in Italy and Germany, all operating in a synchronized manner under the presented geographically-distributed setup. The results show that the proposed architecture is flexible enough to be used for modeling all the different case studies; moreover, they highlight the significant contribution that the GD-RTDS methodology can give in informing and driving energy transition policies and the fundamental role of power systems to spearhead the complete decarbonization of the energy sector.

Behavioral science labs: How to solve the multi-user problem

When lab resources are shared among multiple research projects, issues such as experimental integrity, replicability, and data safety become important. Different research projects often need different software and settings that may well conflict with one another, and data collected for one project may not be safeguarded from exposure to researchers from other projects. In this paper we provide an infrastructure design and an open-source tool, labManager, that render multi-user lab facilities in the behavioral sciences accessible to research projects with widely varying needs. The solutions proposed ensure ease of management while simultaneously offering maximum flexibility by providing research projects with fully separated bare metal environments. This solution also ensures that collected data is kept separate, and compliant with relevant ethical standards and regulations such as General Data Protection Regulation (GDPR) legislation. Furthermore, we discuss preconditions for running shared lab facilities and provide practical advice.