Laboratory Facilities Research Articles

Long short term memory networks for predicting resilient Modulus of stabilized base material subject to wet-dry cycles.

The resilient modulus (MR) of different pavement materials is one of the most important input parameters for the mechanistic-empirical pavement design approach. The dynamic triaxial test is the most often used method for evaluating the MR, although it is expensive, time-consuming, and requires specialized lab facilities. The purpose of this study is to establish a new model based on Long Short-Term Memory (LSTM) networks for predicting the MR of stabilized base materials with various additives during wet-dry cycles (WDC). A laboratory dataset of 704 records has been used using input parameters, including WDC, ratio of calcium oxide to silica, alumina, and ferric oxide compound, Maximum dry density to the optimal moisture content ratio (DMR), deviator stress (σd), and confining stress (σ3). The results demonstrate that the LSTM technique is very accurate, with coefficients of determination of 0.995 and 0.980 for the training and testing datasets, respectively. The LSTM model outperforms other developed models, such as support vector regression and least squares approaches, in the literature. A sensitivity analysis study has determined that the DMR parameter is the most significant factor, while the σd parameter is the least significant factor in predicting the MR of the stabilized base material under WDC. Furthermore, the SHapley Additive exPlanations approach is employed to elucidate the optimal model and examine the impact of its features on the final result.

Further experimental analysis of undershot water wheels towards the development of a prototype model

ABSTRACT The current research aims to analyse the effect of the number and shape of the blades and the curvature of the flume bottom on the performance curves of undershot water wheels, based on experimental tests conducted in a fully instrumented laboratory facility. Six wheels are tested: four wheels with plane blades (16, 24, 36, 48) and two with 24 curved blades for two flume bottom configurations. Torque, mechanical power and mechanical efficiency performance curves are determined for several rotational speed and flow rate values. Results demonstrate that the maximum efficiency is achieved for the 36-plane blade wheel, the curved flume bottom reduces water losses under the wheel and increases efficiency, and the blades’ shape strongly influences the wheel efficiency. Non-dimensional performance curves are provided to generalise the results. This research provides relevant contributions towards the development of a low-cost energy recovery solution to be applied in water infrastructures.

Board Performance of Fisheries Graduates: Logistic Regression Analysis for Curriculum Enhancement

This study investigates the factors affecting the board performance of Bachelor of Science in Fisheries (BSFi) graduates in the fisheries technologists licensure exams in a specific region of the Philippines. The impetus for this research arose from observed variations in exam performance among graduates, prompting an examination of educational and institutional influences on success. The study used a purposive sampling technique to gather data from 138 respondents across State Universities and Colleges (SUCs) who took their first licensure exam from October 2018 to October 2023. A validated online survey questionnaire assessed academic performance, teaching methods, curriculum, faculty qualifications, library resources, laboratory facilities, and student characteristics. Logistic regression analysis was employed to analyze the relationship between these independent variables and licensure exam performance. The findings of this study are of profound importance, as they reveal strong correlations between faculty qualifications and exam outcomes, emphasizing the need for continuous professional development. Moreover, the study identifies improved laboratory facilities and a robust curriculum as critical factors contributing to student success. These insights have the potential to significantly impact stakeholders, informing them about essential areas for intervention and improvement. Ultimately, they aim to elevate the educational experience and licensure performance of BSFi graduates, thus supporting their professional trajectories in the fisheries sector.

Evaluating the Teaching of Physical Science Practical Work at a Selected Resource-Constrained Secondary School in Rundu Circuit, Kavango East Region

This study evaluated the teaching of Physical Science practical work at a resource-constrained secondary school in the Rundu Circuit, Kavango East region in Namibia. The research objectives were to assess the impact of practical work on learners’ academic performance in a resource-constrained community, identify the challenges faced by both teachers and learners in conducting practical activities, and propose strategies to address these challenges and improve the overall quality of science education. A qualitative research design was employed, with data collected through semi-structured interviews involving four Physical Science teachers. The interviews were analyzed using thematic analysis, with themes aligned to the research objectives. The findings revealed that the state of science practical work was poor due to a lack of sufficient laboratory equipment and resources. Other significant challenges identified included limited laboratory space, inadequate time allocation, overcrowded classrooms, a lack of motivation among learners, and insufficient background knowledge on practical work for learners. These challenges collectively impeded effective teaching and learning of science practical work. To overcome these issues, the study recommended providing adequate laboratory materials and constructing an additional laboratory to alleviate overcrowding. The study also further recommended the employment of laboratory technicians to assist teachers in organizing, planning and conducting practical activities more efficiently. Furthermore, the study suggested forming partnerships between schools to share laboratory facilities, as per Key Area 7 of the National Standard and Performance Indicators for Schools in Namibia, which emphasizes the importance of collaborative linkages within educational communities. By implementing these strategies, it was concluded that the quality of Physical Science practical work in resource-constrained schools could be significantly enhanced, leading to improved learner outcomes.

Evaluation of a Novel Mechanical Device for the Production of Microfragmented Adipose Tissue for Veterinary Regenerative Medicine: A Proof-of-Concept.

Therapies based on mesenchymal stromal cells (MSCs) have become one of the most significant advancements in veterinary regenerative medicine. The isolation of MSCs is usually performed by enzymatic digestion and requires variable times for cell expansion. In addition, these procedures need to be performed in specialized laboratory facilities. An alternative approach to in vitro-expanded MSC therapy is the use of microfragmented adipose tissue (microfat), which is a rich source of cells and growth factors from the stromal vascular fraction. Recent clinical studies support its safety and efficacy in the treatment of musculoskeletal disorders and wound healing. The aim of the present work was to characterize the microfragmented adipose tissue obtained by a new mechanical device, which provides sterile tissue that is ready for use in the clinic by the veterinarian, avoiding the need for specialized laboratory facilities. Microfat-derived MSCs were compared with enzymatically isolated MSCs in terms of their phenotypic characterization, growth rate and differentiation potential. Conditioned medium derived from microfat culture was evaluated for its ability to promote MSC vitality. No differences were observed between MSCs obtained through mechanical fragmentation and those derived from collagenase digestion of adipose tissue, suggesting that the device could serve as a practical source of microfragmented adipose tissue for use in veterinary clinics.

Manajemen Laboratorium IPA di Madrasah Tsanawiyah Negeri 02 Lombok Timur

A vital component of teaching science is laboratory administration, which is the endeavor to oversee a laboratory and learning activities. Science laboratory management is related to the managers and users of laboratory facilities and practical activities carried out in the laboratory. This study aimed to determine how planning, organizing resources and assets, implementation, supervision, and impacts arise in the management of science laboratories at MTs.N 02 East Lombok. In this study, documentation, observation, and interviews are the methods used to collect data. The results of the study are that the planning stage is quite good, the existence of short-term, medium-term, and long-term programs evidences this. Short-term programs are planned to be completed within a day, a week, or even a month. At the same time, the medium term is planned to be completed during a quarter to a semester. The Organizational Stage is the management of resources, tools, and materials available in the science laboratory. An organizational structure for managing science laboratories has been formed for the organization of resources. The Implementation Stage is the operational stage of the plan that has been scheduled. This stage is very urgent because it determines the success of achieving goals. Related to the implementation stage, the science laboratory manager at MTs. N. 02 East Lombok has done many things such as inventorying available tools and materials, making regulations, and utilization schedules that are synchronized with the madrasah program. The supervision stage functions as a controller and evaluator of the success of the implementation of laboratory management as a whole. The impacts that arise from laboratory management have been implemented well at MTs. N. 02 East Lombok.

Rapid reagent-free anaemia screening using plant-derived “HemoQR” paper-strips and smartphone: A study on 200 human subjects

Detecting anaemia in resource-limited settings is challenging due to limited access to diagnostic tools, healthcare infrastructure, and trained personnel, often resulting in underdiagnosis and delayed treatment. This study aims to evaluate HemoQR, a novel point-of-care (POC) diagnostic method for haemoglobin (Hb) level detection using a smartphone-based system integrated with a paper strip without needing any reagent or additional auxiliary device. The goal is to assess the efficacy of this portable, user-friendly, rapid and accurate alternative for Hb measurement in large-scale intervention programmes such as Anaemia-mukt Bharat (AMB), which is crucial for diagnosing anaemia and monitoring various health conditions. The method involves the transfer of one drop of the blood sample to a paper strip that is made indigenously using plant based cellulosic sources for optimal functionality. The colour signal around the test spot is quantitatively analysed using a smartphone camera equipped with a custom application. The application processes the images through a machine learning algorithm to determine the Hb concentration. The system was evaluated in a clinical trial involving 200 participants, comparing its performance with the gold-standard laboratory Hb assay using automated haematology analyser. The smartphone-based method demonstrated high sensitivity (87.09 %) and specificity (96.11 %) for anaemia screening, as compared with laboratory results. The average detection time was significantly reduced to under q minute, and the system showed excellent user-friendliness and portability with no auxiliary device needed beyond the smartphone. Clinical trial results indicated that the device was accurate and reliable across different Hb levels. Summarily, HemoQR evidenced to be a promising tool for POC diagnostics. Its high accuracy, rapid results, and ease of use make it suitable for both clinical settings and remote areas with limited access to laboratory facilities. Future research will prioritize further validation and integration of the anaemia screening outcome with electronic health records from a large population cohort. This will help identify early indicators and signals of numerous emerging disease burdens that could escalate into secondary and tertiary health crises if not addressed in time.

Application ICP-OES to Multielement Analysis on Plastic Waste and Blends with Vacuum Gas Oil: Developing a Sample Preparation Protocol

This paper introduces a new methodology for a routine metal analysis of plastic waste (PW) and PW blended with petroleum feedstock such as vacuum gas oil and VGO (PW/VGO). For such purposes, recycled polyethylene and polypropylene plastic were selected to mimic the potential feeds to be integrated at the Fluid Catalytic Cracking unit (FCC) to produce valuable products. Elements such as P, Ca, Al, Mg, Na, Zn, B, Fe, Ti, and Si were included in the method development. Different sample preparation methods were evaluated, such as microwave-assisted acid digestion (MWAD) and dry/wet ashing, followed by a fusion of the ash with lithium borate flux. Some PW homogenization pretreatments, such as cryogenic grinding and hot press molding, were also covered. The finding of this work suggests that MWAD with HNO3 and H2O2 is adequate for both types of samples and is the quickest sample preparation; however, the sample needed to be homogenized, and recoveries for Si and Ti may be biased for PW due to the limited solubilities of these elements in the nitric acid media. Carbon removal is required before fusion sample preparation and analysis due to the amount of carbon in PW samples. The sample needed to be homogenized for wet ash fusion but not for the pre-ash (dry) method. A benefit to the damp ash pretreatment is that the ash for the sample was created in the same crucible used for fusion digestion, avoiding material loss during sample management. Fusion from wet ash or carbon removal allowed for better acid solubility for Si and Ti in PW. The results of the PW samples evaluated matched well with those of both sample preparation methodologies. For most elements, precision was <10% regardless of the sample preparation; however, Fe and P had some variation using wet ash fusion, possibly due to contamination in an open digestion system or variation due to being close to the method limit of quantification (LOQ). The methodology reported here is robust enough to be implemented as routine analysis in any laboratory facility.

Effects of Stretch-Bending Straightening on the Tensile Properties of Cold Rolled Packaging Steel

Achieving perfect flatness, tension-free surfaces, and exceptional resistance to spring back are important characteristics of packaging steel, setting the standard for high-quality material performance. To guarantee these crucial parameters, the implementation of the stretch-bending process at the final stage of the production route is indispensable. Besides improving the flatness properties, the induced plastic deformation results in an accompanying change in the mechanical properties. This investigation focuses on understanding this change in mechanical properties due to different stretch-bending straightening process parameters. A multivariate predictive model is created to calculate a process window for achieving the desired flatness and also mechanical properties in the production of packaging steel. This model is validated by experiments with a laboratory facility.

Bridging to Commercialization: Record-Breaking of Ultra-Large and Superior Cyclic Stability Tungsten Oxide Electrochromic Smart Window.

Electrochromic smart windows (ESWs) can significantly reduce energy consumption in buildings, but their cost-effective, large-scale production remains a challenge. In this study, the instability of black phosphorus is leveraged to induce the growth of the tungsten oxide film through its decomposition process, inspired by the 2D material-assisted in situ growth (TAIG) method. This approach results in the preparation of large-scale, high-performance WO3-x·nH2O (n<2) films. Characterization techniques and DFT calculations confirm efficient regulation ofstructural water and oxygen vacancies during TAIG preparation. The WO3-x·nH2O films exhibit excellent electrochromic (EC) properties, including high transmittance modulation (74.2%@1100nm), fast switching time (tc=5.5s, tb=3.8s), high coloration efficiency (124.7cm2C-1), and superior cyclic stability (transmittance modulation retained 94.7% after 20000 cycles). Ultra-largeWO3-x·nH2O film are prepared via a simple immersion process, and fabricated into a large-area ESW under facile laboratory conditions, demonstrating the economic and practical feasibility of this approach in industrial-scale production. Operated by the intelligent control circuit, the ESWexhibits remarkable EC properties and cyclic stability This research represents a milestone in improving the performance and industrial-scale production of ESWs, bridging the gap to the commercialization of EC technology.

Анкетирование как один из методов оценки качества образовательной деятельности в медицинском учреждении.

The article deals with the issues of training highly qualified specialists (residents) at the Federal State Budgetary Scientific Institution “Central TB Research Institute” (FSBI CTRI). Major changes in the society and development of information technologies require improvement of methods used for education and training of highly qualified specialists and set up new requirements for managing the training process, namely: the use of digital technologies, including distance learning, development of new interactive teaching methods and practicing individualized approach to training. FSBI CTRI trains specialists in the field of phthisiology, pulmonology and surgery using its clinical and laboratory facilities. Through its self-assessment procedure that is part of the accreditation monitoring of educational activities, FSBI CTRI engages the main players of the educational process into the survey aimed at improving training of highly qualified specialists. The authors of the article analyze the results of the questionnaire survey from the CTRI teachers. The data obtained during the survey helped assess the degree of satisfaction with the CTRI system of education among the survey participants, identify the main challenges of educational activities and find solutions.

EXPERIMENTAL RESEARCH OF THE MAGNETIZATION AND DEMAGNETIZATION PROCESSES OF THE VECTOR-CONTROLLED INDUCTION MOTOR

Experimental research of the processes of magnetization and demagnetization for the motionless vector-controlled induction motor was performed, according to a linear law with a variation in the duration of these processes. The methodology of research during which the orthogonal components of the stator and rotor currents were recorded in the rotor flux reference frame, the rotor flux module and the energy of the total losses in the stator and rotor copper is described. The main characteristics and parameters of laboratory equipment and facilities are given. From the point of view of minimizing copper losses, the existence of the optimal duration of the investigated transient processes has been experimentally proven. The results of experimental studies with high accuracy coincide with calculations based on previously obtained analytical dependencies, which confirms the admissibility of the assumptions made during theoretical studies. References 10, figures 7, tables 2.

Sputum and tongue swab molecular testing for the in-home diagnosis of tuberculosis in unselected household contacts: a cost and cost-effectiveness analysis.

Delayed and missed diagnosis are a persistent barrier to tuberculosis control, partly driven by limitations associated with sputum collection and an unmet need for decentralized testing. Household contact investigation with point-of-care testing of non-invasive specimens like tongue swabs are hitherto undescribed and may be a cost-effective solution to enable community-based active case finding. In-home, molecular point-of-care testing was conducted using sputum and tongue specimens collected from all household contacts of confirmed tuberculosis cases. A health economic assessment was executed to estimate and compare the cost and cost-effectiveness of different in-home, point-of-care testing strategies. Incremental cost effectiveness ratios of strategies utilizing different combination testing algorithms using sputum and/or tongue swab specimens were compared. The total implementation cost of delivering the standard of care for a 2-year period was $84 962. Strategies integrating in-home point-of-care testing ranged between $87 844 - $93 969. The cost-per-test for in-home, POC testing of sputum was the highest at $20·08 per test. Two strategies, Point-of-Care Sputum Testing and Point-of-Care Combined Sputum and Individual Tongue Swab Testing were the most cost-effective with ICERs of $543·74 and $547·29 respectively, both below a $2,760 willingness-to-pay threshold. An in-home, point-of-care molecular testing strategy utilizing combination testing of tongue swabs and sputum specimens would incur an additional 10.6% program cost, compared to SOC, over a 2-year period. The increased sample yield from tongue swabs combined with immediate result notification following, in-home POC testing would increase the number of new TB cases detected and linked to care by more than 800%. Evidence before this study: We searched PubMed for original research published between January 1, 1950 and June 30, 2024 that evaluated the cost-effectiveness of in-home POC molecular testing, as part of HCI strategies for tuberculosis. PubMed search terms used included ["household contact investigation" OR "household contact tracing"] AND "tuberculosis" AND "cost-effectiveness". The search revealed 8 studies, of which one was removed as HCIs were leveraged for the provision of short course preventative therapy and not tuberculosis testing. None of the studies were conducted in South Africa. All seven remaining studies relied on a hub-and-spoke model of sputum collection and transportation with sputum tested at a centralized laboratory facility. Although active case finding strategies like HCIs are endorsed by the WHO to improve early case detection and treatment initiation, limited research has been done to assess its cost-effectiveness in low- and middle-income countries.Added value of this study: To our knowledge, this is the first example of in-home molecular point-of-care (POC) testing as part of HCI. The use of primary data to estimate and compare the incremental cost effectiveness of different combination, in-home testing strategies utilizing alternative sample types equips policy makers with a selection of strategy options to choose from. The tradeoff between sample types with high collection yield and those with increased accuracy becomes evident in the economic analysis, highlighting the need to consider both yield and accuracy in effective clinical decision making and use-case development. The success of in-home, POC tongue swab testing of all contacts, irrespective of symptom presentation shows great promise for universal testing programs.Implications of all available evidence: Results from our economic modeling provide evidence in support for the integration of in-home, POC tuberculosis (TB) testing during HCI. The use of less invasive tongue swab samples to increase sample yield in the absence of sputum expectoration highlights the value of combination testing strategies. Immediate result notification resulting from rapid, in-home POC testing shows great promise for increasing early case detection and improving treatment uptake. In-home, POC testing strategies, when incorporated into HCI could curb ongoing community transmission and reduce the overall burden of TB. Considerations for adopting novel POC testing strategies in future active case finding programs like HCI should strongly be considered. We evaluated the cost-effectiveness of in-home, point-of-care TB testing of household contacts. The findings indicate that combined testing strategies using tongue swab and sputum specimens could significantly increase TB case detection, with modest additional program costs.

Application of Data Mining to Measure the Level of Satisfaction with Public Facilities and Services at STMIK Kaputama Binjai Using Linear Regression Method

This study aims to analyze the level of satisfaction of STMIK Kaputama Binjai students with physical facilities (classrooms, laboratories, prayer rooms, wifi) and general services (administration, academic guidance, library, security, campus cleanliness) using multiple linear regression methods. Data were collected through questionnaires from students in the 2022/2023 academic year. The results showed that both variables have a significant effect on student satisfaction, with a regression coefficient of physical facilities of 0.40 and general services of 0.59, indicating that general services have a greater impact. Prediction of student satisfaction reached an accuracy level of 98% with a Mean Absolute Percentage Error (MAPE) value of 2%. Laboratory facilities and internet access (wifi) are the dominant factors affecting satisfaction. Based on these findings, improvements in both aspects are recommended to increase student satisfaction and institutional competitiveness.

Management of Emergency Department Services at a Secondary Level Hospital

Background: The primary purpose of the Emergency Department (ED) is to provide immediate medical attention and resuscitation to seriously ill patients. Prompt treatment and efficient service management play crucial roles in preventing fatalities and disabilities. Objective: To evaluate the management of emergency department services at a secondary-level hospital in Bangladesh. Methodology: A descriptive cross-sectional study was carried out in the emergency department at Narayanganj General Hospital from January to December 2018. The study involved 229 participants, including both service recipients and providers. Data was collected using a pre-tested, semi-structured questionnaire administered through face-to-face interviews. Results: The majority of participants easily identified the ED room (82.3%) and waited ≤15 minutes (91.2%) to see a doctor. They also felt that doctors listened attentively to their complaints (88.9%) and behaved well (92.5%). Service providers' behavior was also positively rated by patients, with 90.8% expressing satisfaction. Most patients did not receive all prescribed medicines from the pharmacy (81.4%), expressed dissatisfaction with the cleanliness of the environment (52.1%), and facilities in the waiting room (60.0%). Despite these concerns, they mostly stated satisfaction with the treatment received (51.6%) and the overall management of the ED (45.1%). Service providers identified several shortcomings across multiple areas, such as laboratory facilities (78.6%), staffing levels of doctors (78.6%), nurses (64.3%), and supporting staff (85.7%). Despite these challenges, they expressed satisfaction (64.3%) with their roles. Conclusion: The study demonstrated that both service recipients and service providers were satisfied with the management of medical services. However, hospital administration and health service providers should prioritize resolving deficiencies in order to improve patient satisfaction more substantially. Journal of Monno Medical College June, 2024; 10 (1):36-42

Optimizing Science Process Skills Through the Use of Simple Steam Power Plant Models in Middle School Physics Education

This study aims to evaluate the effectiveness of using a simple steam power plant prop in improving the science process skills of eighth grade students at Pesantren Guppi Samata Junior High School. The importance of this study lies in the need for innovative teaching methods that can improve students' engagement and understanding in science education, especially considering the limitations of traditional laboratory resources. A quasi-experimental design with a pretest-posttest control group was used, involving purposive sampling of two groups: an experimental group that used a simple steam power plant prop and a control group that did not use the prop. Data were collected through a structured multiple-choice test that assessed various aspects of science process skills and were analyzed using descriptive statistics, normality tests, and independent t-tests. The findings showed that the experimental group significantly outperformed the control group in the posttest (p = 0.000), indicating that the simple steam power plant prop effectively facilitates better understanding of scientific concepts through hands-on learning. The novelty of this study lies in its focus on the use of simple and accessible teaching props to improve practical skills in science education. The results of this study suggest that incorporating these tools into teaching can improve educational outcomes, and offer a valuable resource for educators facing limited laboratory facilities.

Detection of Avian Leukosis Virus Subgroup J (ALV-J) Using RAA and CRISPR-Cas13a Combined with Fluorescence and Lateral Flow Assay.

Avian Leukosis Virus (ALV) is a retrovirus that induces immunosuppression and tumor formation in poultry, posing a significant threat to the poultry industry. Currently, there are no effective vaccines or treatments for ALV. Therefore, the early diagnosis of infected flocks and farm sanitation are crucial for controlling outbreaks of this disease. To address the limitations of traditional diagnostic methods, which require sophisticated equipment and skilled personnel, a dual-tube detection method for ALV-J based on reverse transcription isothermal amplification (RAA) and the CRISPR-Cas13a system has been developed. This method offers the advantages of high sensitivity, specificity, and rapidity; it is capable of detecting virus concentrations as low as 5.4 × 100 copies/μL without cross-reactivity with other avian viruses, with a total testing time not exceeding 85 min. The system was applied to 429 clinical samples, resulting in a positivity rate of 15.2% for CRISPR-Cas13a, which was higher than the 14.7% detected by PCR and 14.2% by ELISA, indicating superior detection capability and consistency. Furthermore, the dual-tube RAA-CRISPR detection system provides visually interpretable results, making it suitable for on-site diagnosis in remote farms lacking laboratory facilities. In conclusion, the proposed ALV-J detection method, characterized by its high sensitivity, specificity, and convenience, is expected to be a vital technology for purification efforts against ALV-J.

Predictor of low academic achievement among Dilla university students, southern Ethiopia, 2024

IntroductionIn Ethiopia, despite its growing higher education sector, student achievement rates remain concerningly low. Understanding the multifaceted factors influencing academic performance is crucial for improving educational equity and quality. This study delves into potential predictors of academic achievement among Ethiopian higher education students, examining individual characteristics, institutional elements, and broader socioeconomic influences.MethodologyThis survey enrolled 362 respondents and was conducted from December 7, 2023 till January 22, 2024. Simple random sampling, validated assessment tools and online data collection methods were employed to select and collect information from respondents. Data entry and analysis was done using Epi-info 7.0 and SPSS 25, respectively. Logistic regression analysis method was used to determine the association between the outcome and independent variable.ResultThe current results show that 166 (45/9%) of participants have GPAs below 3.18. Gender, social sciences/humanities or business/economics majors, suboptimal class environments, inadequate laboratory facilities, chronic illness, class sizes, low emotional coping skills, poor academic self-perception, and high social media use emerged as significant predictors of low academic achievement.ConclusionThis study identified factors associated with academic achievement. Female students, optimal learning environments, and smaller class sizes were linked to better performance, while social sciences/humanities or business/economics, inadequate facilities, and high social media use increased the risk of low achievement. Personal characteristics like emotional coping, self-perception, and chronic illness also played a role. These findings suggest interventions targeting individual and environmental factors could improve student outcomes.

Optimizing Science Laboratory Management for Enhanced Student Learning Outcomes

Background: Laboratory management is vital for improving science education, yet many institutions struggle to optimize these resources. Specific Background: Effective management enhances student interest and practical skills in natural sciences, but inadequate management leads to underutilization of laboratory facilities, negatively impacting learning experiences. Knowledge Gap: While the importance of laboratory management is acknowledged, studies on specific practices and their effects on student engagement in natural science remain limited. Aims: This study explores management practices in a natural science laboratory and their implications for educational outcomes. Results: Findings reveal that planning, organization, implementation, supervision, and evaluation are poorly executed, resulting in incomplete documentation and minimal follow-up. Although practical activities spark student interest, systemic issues hinder their effectiveness. Novelty: The research provides new insights into the operational challenges of laboratory management in a specific educational context. Implications: Enhanced management strategies are necessary to improve resource allocation and laboratory utilization, fostering student engagement and skill development in natural sciences. Highlights : Critical Role: Effective laboratory management enhances student interest and practical skills in science education. Systemic Issues: Inadequate management practices lead to underutilization of laboratory resources, negatively impacting learning experiences. Need for Improvement: Enhanced strategies are essential for optimizing resource allocation and fostering student engagement in natural sciences. Keywords: laboratory management, science education, student engagement, practical skills, educational outcomes

A-059 Validation of self-collected capillary dried blood spot collection for clinical HbA1c testing

Abstract Background The increased prevalence of diabetes in socially disadvantaged populations requires solutions that are amenable to these communities. Unmanaged diabetes can cause cardiovascular disease, kidney damage, blindness, and limb amputation. Diabetes is traditionally diagnosed using fasting/oral glucose tolerance or hemoglobin A1c (HbA1c) tests; both of which require access to medical and laboratory facilities. Self-collected capillary dried blood spot (DBS) testing for HbA1c could alleviate health disparities by offering access to low cost and minimally invasive remote collection. The objective of this study was to validate self-collected capillary DBS specimens for clinical HbA1c testing. Methods DBS HbA1c was measured using the hemolysate application of the Roche cobas c513 Tina-quant Hemoglobin A1cDx Gen.3 assay. Calibration was performed according to the manufacturer’s package insert. Patient samples were prepared by collecting two 3mm DBS punches. Non-biological standards were prepared by placing two blank 3mm DBS punches into an empty tube and adding 10uL of standard. After 24 hours, samples were resuspended in 750uL of hemolyzing reagent and incubated for 75 minutes at room temperature (RT) while shaking at 800 RPM. Preliminary data showed that non-biological standards spotted onto DBS cards did not behave like real patient samples. A correction factor (CF) was derived (n=134) and challenged (n=20) to account for the systematic bias observed between matched patient DBS and venous specimens. Imprecision was established by running two patient DBS specimens as controls. Accuracy was evaluated using residual College of American Pathologists (CAP) proficiency testing (PT) material (n=32). Linearity was confirmed by running 5 levels of Maine Standards Linearity HbA1c Kit in triplicate. Stability of the DBS specimen and the hemolysate supernatant extracted from the DBS specimen were observed at RT. Results Specimens tested between venous and DBS HbA1c to derive the CF (n=134) had a bias of 0.26% (4.32%) and a linear equation of (y=0.938x +0.631; R=0.996). The resulting CF is: reported HbA1c% = (DBS HbA1c% - 0.631)/0.938. Matched venous and DBS specimens that challenged the CF (n=20) had a bias of -0.10% (-1.83%) and a linear equation of (y=1.06x -0.41; R=0.988). Imprecision was 2.3% and 1.3% for patient DBS control specimens with mean concentrations of 5.3% and 5.9%, respectively. Residual CAP PT samples (n=32) had an average bias of -0.05% (-0.40%). DBS HbA1c concentrations from 4.2-15.5% were linear (y=0.903x +1.15; R=0.999). The DBS specimen is stable for 60 days at RT and the hemolysate supernatant is stable for at least 10 hours at RT. Conclusions Self-collected capillary DBS specimens are acceptable for the clinical measurement of HbA1c, and effectively increase accessibility to diabetes screening and monitoring. Applying a CF to DBS patient samples removes the systematic bias observed between matched venous and DBS samples. Non-biological standards and capillary whole blood do not behave the same on DBS cards. Alternative PT for DBS specimens should be investigated as at-home collection advances.