Laboratory-based Studies Research Articles

Going against the flow: bumblebees prefer to fly upwind and display more variable kinematics when flying downwind.

Foraging insects fly over long distances through complex aerial environments, and many can maintain constant ground speeds in wind, allowing them to gauge flight distance. Although insects encounter winds from all directions in the wild, most lab-based studies have employed still air or headwinds (i.e. upwind flight); additionally, insects are typically compelled to fly in a single, fixed environment, so we know little about their preferences for different flight conditions. We used automated video collection and analysis methods and a two-choice flight tunnel paradigm to examine thousands of foraging flights performed by hundreds of bumblebees flying upwind and downwind. In contrast to the preference for flying with a tailwind (i.e. downwind) displayed by migrating insects, we found that bees prefer to fly upwind. Bees maintained constant ground speeds when flying upwind or downwind in flow velocities from 0 to 2 m s-1 by adjusting their body angle, pitching down to raise their air speed above flow velocity when flying upwind, and pitching up to slow down to negative air speeds (flying backwards relative to the flow) when flying downwind. Bees flying downwind displayed higher variability in body angle, air speed and ground speed. Taken together, bees' preference for upwind flight and their increased kinematic variability when flying downwind suggest that tailwinds may impose a significant, underexplored flight challenge to bees. Our study demonstrates the types of questions that can be addressed with newer approaches to biomechanics research; by allowing bees to choose the conditions they prefer to traverse and automating filming and analysis to examine massive amounts of data, we were able to identify significant patterns emerging from variable locomotory behaviors, and gain valuable insight into the biomechanics of flight in natural environments.

Your suffering is my stressor: Proinflammatory gene expression rises with spousal distress in middle-aged and older couples

Marital quality shares ties to inflammation-related conditions like cardiovascular disease and diabetes. Lab-based studies implicate hostility during marital conflict as a mechanism via inflammatory reactivity, but little attention has been paid to the inflammatory aftermath of other marital exchanges. A spouse’s emotional distress is an important but overlooked context for middle-aged and older couples, as conflict declines and networks shrink. To examine the links of spousal distress to changes in proinflammatory gene expression, 38 adults ages 40–81 witnessed their spouse relive an upsetting personal memory aloud, rated their mood before and after, and provided blood samples at baseline and twice post-task; they also shared their own upsetting memory and discussed a marital problem in the interim. Those whose spouse disclosed their upsetting memory with greater emotional intensity showed larger elevations in proinflammatory gene expression 30–40 min and 80–90 min after the task. The association replicated for listeners whose negative mood increased more in response to spousal disclosure. Findings were robust to behavior in the other emotional tasks, race, gender, age, alcohol, smoking, comorbidities, and sagittal abdominal diameter. These novel results identify spousal distress as a key marital context that may escalate inflammation-related health risks.

Insights of fluid dynamics in an optimally shaped root canal system

Dear Editor, Fluid flow patterns are investigated and understood only at in vitro levels through laboratory-based studies.[1] Various physical parameters play a crucial role in enhancing the irrigant flow in a prepared canal space. Ideally, the physiological limit of the irrigant must be restricted within the canal space and also it might not be clinically possible to specifically determine the exact value when the irrigant exceeds the optimal flow rate. Laboratory-based studies proved 1–4 ml/min to be the optimal flow rate to prevent inherent extrusions and apical pressures.[2,3] Although the in vitro literature focused more on syringe needle irrigation,[1] in routine clinical practice with the use of manual needle irrigation, it might not be possible to obtain maximum disinfection. The current clinical literature is now more focused on the efficacy of activation systems with less preference to manual irrigation techniques.[4,5] Previous study showed 30G side vented needles to be the most frequently used[6] and induce the least pressure at a specified irrigant flow rate.[1] However still, the existing literature evidence has not given insights into the fluid dynamics of various activation systems and also the complex nature of the irrigant flow pattern is unexplored. As the irrigant flow rate cannot be standardized on activation, there is an urge for future research which specifically investigates the irrigation dynamics of using various activation devices. Hence, the tendency of irrigant extrusions with various activation devices and the complexity of fluid dynamics are yet to be investigated, especially considering the various physical parameters, including the velocity, turbulence, and shear wall stress considering the induced apical pressures. Financial support and sponsorship Nil. Conflicts of interest There are no conflicts of interest.

Exposure to the persistent organic pollutant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, dioxin) disrupts development of the zebrafish inner ear

Dioxins are a class of highly toxic and persistent environmental pollutants that have been shown through epidemiological and laboratory-based studies to act as developmental teratogens. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), the most potent dioxin congener, has a high affinity for the aryl hydrocarbon receptor (AHR), a ligand activated transcription factor. TCDD-induced AHR activation during development impairs nervous system, cardiac, and craniofacial development. Despite the robust phenotypes previously reported, the characterization of developmental malformations and our understanding of the molecular targets mediating TCDD-induced developmental toxicity remains limited. In zebrafish, TCDD-induced craniofacial malformations are produced, in part, by the downregulation of SRY-box transcription factor 9b (sox9b), a member of the SoxE gene family. sox9b, along with fellow SoxE gene family members sox9a and sox10, have important functions in the development of the otic placode, the otic vesicle, and, ultimately, the inner ear. Given that sox9b is a known target of TCDD and that transcriptional interactions exist among SoxE genes, we asked whether TCDD exposure impaired the development of the zebrafish auditory system, specifically the otic vesicle, which gives rise to the sensory components of the inner ear. Using immunohistochemistry, in vivo confocal imaging, and time-lapse microscopy, we assessed the impact of TCDD exposure on zebrafish otic vesicle development. We found exposure resulted in structural deficits, including incomplete pillar fusion and altered pillar topography, leading to defective semicircular canal development. The observed structural deficits were accompanied by reduced collagen type II expression in the ear. Together, our findings reveal the otic vesicle as a novel target of TCDD-induced toxicity, suggest that the function of multiple SoxE genes may be affected by TCDD exposure, and provide insight into how environmental contaminants contribute to congenital malformations.

A narrative review of precision medicine in neonatal sepsis: genetic and epigenetic factors associated with disease susceptibility.

Neonatal sepsis is a dysregulated host response to an infectious agent that results in severe morbidity and mortality among neonates worldwide. Given the complex and heterogenous nature of neonatal sepsis, early diagnosis and individualized treatment remain challenges for clinicians despite clinical advance. Epidemiological studies on twins suggest that hereditary factors act in conjunction with environmental factors to affect neonatal sepsis susceptibility. However, little is known about hereditary risks at present. This review aims to elucidate neonatal hereditary predisposition to sepsis and outline thoroughly the genomic landscape underlying neonatal sepsis, which may, to a large extent, facilitate precision medicine in this area. PubMed was searched for all published literature relating to neonatal sepsis using Medical Subject Headings (MeSH) terms, with a focus on hereditary factors. Without any restriction on article type, articles published in English prior to June 1, 2022, were retrieved. Additionally, pediatric, adult, and animal- and laboratory-based studies were reviewed wherever possible. This review provides a detailed introduction regarding the hereditary risk of neonatal sepsis in terms of genetics and epigenetics. Its findings demonstrate the potential for translation to precision medicine, where risk stratification, early diagnosis, and individualized interventions might be matched to the certain population. This review delineates the comprehensive genomic landscape underpinning inherent susceptibility to neonatal sepsis, allowing future studies to integrate hereditary information into a routine protocol and drive precision medicine from the bench to the bedside.

Eyewitness confidence and decision time reflect identification accuracy in actual police lineups.

Although there are many lab-based studies demonstrating the utility of confidence and decision time as indicators of eyewitness accuracy, there is almost no research on how well these variables function for lineups in the real world. In two experiments, we examined confidence and decision time associated with real lineups that had been conducted using research-based recommendations. We expected that how confident an eyewitness sounded and how quickly that eyewitness made their identification would be associated with whether that eyewitness identified a suspect or a filler. We also hypothesized that people's interpretations of eyewitness confidence could be easily influenced by additional, biasing information. Using audio recordings of these lineups, we examined (a) participants' subjective ratings of how confident an eyewitness sounded at the time of the identification and (b) objective data regarding how quickly the eyewitness made the identification decision. We also manipulated what additional information, if any, participants received in Experiment 2. In both experiments, decision time and confidence predicted whether the eyewitnesses identified the suspect or a known-innocent filler, and when decision time and confidence diverged, it is likely that the eyewitness identified a filler. In Experiment 2, we found that people's interpretations of eyewitness's confidence statements could be biased. When observers believed that the witness picked a filler rather than a suspect, or vice versa, this changed how confident they thought the witness sounded. Confidence and decision time should both be collected when administering real lineups, but objective decision time data may be the most useful because people's perceptions of confidence are easily altered. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Continuous cultivation of the lithoautotrophic nitrate-reducing Fe(II)-oxidizing culture KS in a chemostat bioreactor.

Laboratory-based studies on microbial Fe(II) oxidation are commonly performed for 5-10 days in small volumes with high substrate concentrations, resulting in geochemical gradients and volumetric effects caused by sampling. We used a chemostat to enable uninterrupted supply of medium and investigated autotrophic nitrate-reducing Fe(II)-oxidizing culture KS for 24 days. We analysed Fe- and N-speciation, cell-mineral associations, and the identity of minerals. Results were compared to batch systems (50 and 700 mL-static/shaken). The Fe(II) oxidation rate was highest in the chemostat with 7.57 mM Fe(II) d-1 , while the extent of oxidation was similar to the other experimental setups (average oxidation of 92% of all Fe(II)). Short-range ordered Fe(III) phases, presumably ferrihydrite, precipitated and later goethite was detected in the chemostat. The 1 mM solid phase Fe(II) remained in the chemostat, up to 15 μM of reactive nitrite was measured, and 42% of visualized cells were partially or completely mineral-encrusted, likely caused by abiotic oxidation of Fe(II) by nitrite. Despite (partial) encrustation, cells were still viable. Our results show that even with similar oxidation rates as in batch cultures, cultivating Fe(II)-oxidizing microorganisms under continuous conditions reveals the importance of reactive nitrogen intermediates on Fe(II) oxidation, mineral formation and cell-mineral interactions.

Do biotransformation data from laboratory experiments reflect micropollutant degradation in a large river basin?

Identifying a chemical's potential for biotransformation in the aquatic environment is crucial to predict its fate and manage its potential hazards. Due to the complexity of natural water bodies, especially river networks, biotransformation is often studied in laboratory experiments, assuming that study outcomes can be extrapolated to compound behavior in the field. Here, we investigated to what extent outcomes of laboratory simulation studies indeed reflect biotransformation kinetics observed in riverine systems. To determine in-field biotransformation, we measured loads of 27 wastewater treatment plant effluent-borne compounds along the Rhine and its major tributaries during two seasons. Up to 21 compounds were detected at each sampling location. Measured compound loads were used in an inverse model framework of the Rhine river basin to derive k’bio,field values – a compound-specific parameter describing the compounds’ average biotransformation potential during the field studies. To support model calibration, we performed phototransformation and sorption experiments with all the study compounds, identifying 5 compounds that are susceptible towards direct phototransformation and determining Koc values covering four orders of magnitude. On the laboratory side, we used a similar inverse model framework to derive k’bio,lab values from water-sediment experiments run according to a modified OECD 308-type protocol. The comparison of k’bio,lab and k’bio,field revealed that their absolute values differed, pointing towards faster transformation in the Rhine river basin. Yet, we could demonstrate that relative rankings of biotransformation potential and groups of compounds with low, moderate and high persistence agree reasonably well between laboratory and field outcomes. Overall, our results provide evidence that laboratory-based biotransformation studies using the modified OECD 308 protocol and k’bio values derived thereof bear considerable potential to reflect biotransformation of micropollutants in one of the largest European river basins.

Weak gene–gene interaction facilitates the evolution of gene expression plasticity

BackgroundIndividual organisms may exhibit phenotypic plasticity when they acclimate to different conditions. Such plastic responses may facilitate or constrain the adaptation of their descendant populations to new environments, complicating their evolutionary trajectories beyond the genetic blueprint. Intriguingly, phenotypic plasticity itself can evolve in terms of its direction and magnitude during adaptation. However, we know little about what determines the evolution of phenotypic plasticity, including gene expression plasticity. Recent laboratory-based studies suggest dominance of reversing gene expression plasticity—plastic responses that move the levels of gene expression away from the new optima. Nevertheless, evidence from natural populations is still limited.ResultsHere, we studied gene expression plasticity and its evolution in the montane and lowland populations of an elevationally widespread songbird—the Rufous-capped Babbler (Cyanoderma ruficeps)—with reciprocal transplant experiments and transcriptomic analyses; we set common gardens at altitudes close to these populations’ native ranges. We confirmed the prevalence of reversing plasticity in genes associated with altitudinal adaptation. Interestingly, we found a positive relationship between magnitude and degree of evolution in gene expression plasticity, which was pertinent to not only adaptation-associated genes but also the whole transcriptomes from multiple tissues. Furthermore, we revealed that genes with weaker expressional interactions with other genes tended to exhibit stronger plasticity and higher degree of plasticity evolution, which explains the positive magnitude-evolution relationship.ConclusionsOur experimental evidence demonstrates that species may initiate their adaptation to new habitats with genes exhibiting strong expression plasticity. We also highlight the role of expression interdependence among genes in regulating the magnitude and evolution of expression plasticity. This study illuminates how the evolution of phenotypic plasticity in gene expression facilitates the adaptation of species to challenging environments in nature.

Tooth Bleaching: A bibliometric analysis of the top 100 most-cited papers.

This study analyzes the characteristics of the top 100 most-cited papers related to tooth bleaching. A literature search was performed on the Web of Science up to March 2022. The number of citations was cross-matched with the citation count on Scopus and Google Scholar. The following data were collected: number and density of citations; authorship; year and journal of publication; study design and thematic; keywords; institution and country of origin. Spearman's correlation and Poisson regression were used to determine associations between the number of citations and study characteristics. The VOSviewer software was used to generate collaborative network maps for the authors and keywords. The number of citations ranged from 66 to 450. Papers were published between 1981 and 2020. The most frequent study design and topic were laboratory-based studies and 'interaction of the bleaching agent with dental tissues', respectively. Cochran M, Loguercio AD, Matis B, Reis A, and Suliman M were the authors with the highest number of papers. The countries with the most papers were the United States of America (USA) (28%) and Brazil (20%). Indiana University and State University of Ponta Grossa were the institutions with the most papers (6% each). There was a very strong correlation among the number of citations of the three databases. The 100 most-cited papers related to tooth bleaching were mainly published by the USA and Brazil, with laboratory-based studies addressing topics related to the effects of bleaching agents on tooth structure being the most prevalent.

Lime slurry treatment of soils developing on abandoned coal mine spoil: Linking contaminant transport from the micrometer to pedon-scale

Historical and abandoned coal mine spoil continues to generate acid- and metal(loid)-rich porewaters and represents a geographically large and diffuse non-point source of contamination to local watersheds. A potentially inexpensive approach to treat these materials and soils developing on them is through the application of lime slurries, to neutralize acidity and encourage the (co)precipitation of metal(loids) with Fe(III)-(oxy)hydroxides, and potentially other metal-oxides and/or Ca-bearing phases. The efficacy of this approach was evaluated through parallel field application and laboratory-based flow-through column experiments. The field site is Huff Run sub-watershed 25 located in Tuscarawas County, Ohio and was chosen in part because it was previously classified as one of the most highly AMD-impacted sub-watersheds in the region. Two locations with historical spoil were chosen and suction lysimeters were installed at 25 and 75 cm depth to monitor porewater composition on two sides at the base of each pile. Half of each slope received seven lime slurry treatments from June through October of 2017. A suite of aqueous (ICP-OES, IC, and TOC-L) and solid phase geochemical and mineralogical approaches (quantitative SEM-EDS and synchrotron μ-XRF) were used to determine how composition, texture, morphology, and spatial distribution of mineral coatings differ in pre- and post-lime treated soils, and how that impacts the distribution and transport of trace metal(loid)s. Mine spoil porewater at site 1 was slightly less alkaline (pH ranging from 7.04 to 7.37) than at site 2 (ranging from 7.55 to 7.71), and average electrical conductivity values at site 1 (316–405 μS cm−1) were slightly lower than at site 2 (358–464 μS cm−1), although differences between the sites were not significant. Porewater pH and electrical conductivity in all lysimeters decreased over the course of the field season but there was no obvious response to lime treatment at either site or any depth. At site 1, both treatment and depth were significant factors affecting Ca, K, Ni, SO42−, and DOC concentrations while only treatment effects were significant for dissolved Al and Cu (p < 0.05). For all soils, there were no trends in metal concentration observed over time although DOC and SO42− decreased over the field season. Pedon-scale changes in metal porewater concentrations in response to treatment were linked to micrometer-scale changes in mineral surface coatings; specifically, higher concentrations of Ca, Fe, Mn, and Zn were observed in the coatings and no changes were observed in Fe redox speciation, whereas total S decreased likely due to oxidation of S in coal fragments. In contrast to the field experiment, the column experiments exhibited a much greater response in effluent composition with respect to lime treatment. The untreated columns had approximately an order of magnitude more H+ leached over the course of the experiment (p < 0.001) and resulted in greater Ca, Al, Cu, and DIC leached and less Mn, Zn, and SO42−. Soils treated with the lime slurry in the column experiments exhibited larger and thicker secondary Fe-coatings, including the addition of Fe-sulfates. Despite clear trends in the laboratory-based column experiment where the lime-to-soil ratio was higher, the effects were either muted or undetected in the field pilot project, suggesting that a higher application rate of lime in the field is needed to achieve a similar effect. This work provides evidence that a less alkaline lime slurry could be a practical and inexpensive method of treating coal mine spoil-impacted soils and represents an important step in linking laboratory-based remediation studies to implemented field-based studies.

Comparing online versus in-person auditory experiments on talker adaptation

Online experiments are increasingly used as an alternative to in-person lab-based experiments. While online experimental platforms offer convenience, conducting auditory experiments online also presents several challenges, including variations in latency and jitter in auditory stimulus presentation across participants’ devices. Therefore, it is important to carefully examine whether response time data collected online are comparable to the reliability and precision of data collected in well-controlled laboratory settings. Here, we directly compared whether well-documented context- and talker-adaptation effects on response times are replicable between the laboratory and an online platform. Participants (N = 58) performed an established word identification task measuring response time in a 2 × 2 manipulation of context (isolated words versus continuous speech) and talker variability (single versus mixed talkers). The results from the laboratory session, but not the online platform, reliably replicated all main and interaction effects found in prior laboratory-based studies. While mean response times were comparable between environments, the variance of online data was significantly greater than in the lab, even after discarding outliers. These results suggest that careful considerations are needed when implementing online experiments that depend on precise auditory timing, particularly in considering how greater measurement error will impact power, sample sizes, and statistical inferences.

A Case for Micromachines in Laboratory-Based DFIG Wind Turbine Systems for Fault Studies

For laboratory-based systems, the selection of components is critical to ensure proper representation of the actual system. In past investigations, researchers made use of small-scale off-the-shelf wound rotor induction machines (WRIMs) to perform laboratory-based studies. However, there are no reports validating their applicability in comparison to using the actual machines. In this regard, this paper investigates the correlation between the dimensional parameters and the electrical asymmetry indicators for a large-scale wind generator and laboratory-based generators. For the latter, a comparison is made between using a standard small-scale off-the-shelf WRIM and a micromachine. It is shown that the harmonic frequencies in a healthy machine and those due to inherent asymmetries appear at the same positions on the stator current spectrum for the three machines. However, there is a difference in the amplitudes of these components, given their dependence on the winding factors for a specific machine. The dimensional analysis techniques used to scale down the large-scale generator into a micromachine ensure that these parameters are related. However, this is not the case for the standard small-scale WRIM. Further work demonstrates the correlation of the fault-related harmonics between the micromachine and the large-scale machine for a rotor inter-turn fault condition. Micromachines are designed to ensure that the time constants are proportional representations of the larger machine, thus providing an accurate reflection of what is expected under transient operating conditions of wind turbines. This would assist in the development of condition monitoring strategies that are suited for the actual systems in the industry.

Study of void space structure and its influence on carbonate reservoir properties: X-ray microtomography, electron microscopy, and well testing

Many carbonate reservoirs containing significant hydrocarbon resources are characterized by complex pore structures and, as a result, heterogeneous distributions of fluids in their pore networks. Accurately assessing the microscopic pore structure characteristics of carbonate rocks and their influence on reservoir properties is essential for the successful development of oil fields. In the studied carbonate reservoirs of three oil fields in the Perm Krai, Russia, complex tectonic activity and multi-stage diagenetic modification have resulted in the formation of a heterogeneous pore network spectrum ranging from macropores to nanometer-scale (<1 μm) pores. Thin sections and computed tomography were applied to obtain the petrography of carbonate rocks, 2D images of a wide range of pores, and a 3D representation of the pore network. Well testing was used to study the pore network structure at the macrolevel. Combining well testing and laboratory-based core studies allowed the structural features of the reservoirs of the three fields under consideration to be characterized in high detail. The presence of magnesium in the mineral composition of the studied limestone decreased its capacitive characteristics. We identify three fundamentally different void space scenarios: (i) void spaces formed only by primary intercrystalline pores, (ii) much larger secondary pores are also present, and (iii) primary and secondary voids connected by a network of partially healed fractures. Such variations in the structure of void space contribute to varying reservoir behavior. The presence of larger voids and, accordingly, higher initial permeability contributes to intense deformation of the void space and permeability decreases with decreasing pressure. The established patterns in this work explain the characteristic dynamics of well flow rates during field development.

Military tactical adaptive decision making during simulated military operational stress is influenced by personality, resilience, aerobic fitness, and neurocognitive function.

The present investigation sought to determine the impact of a 48-h simulated military operational stress (SMOS) on military tactical adaptive decision making, and the influence of select psychological, physical performance, cognitive, and physiological outcome measures on decision making performance. Male (n = 48, 26.2 ± 5.5 years, 177.7 ± 6.6 cm, 84.7 ± 14.1 kg.) subjects currently serving in the U.S. military were eligible to participate in this study. Eligible subjects completed a 96-h protocol that occurred over five consecutive days and four nights. Day 2 (D2) and day 3 (D3) consisted of 48-h of SMOS wherein sleep opportunity and caloric needs were reduced to 50%. Differences in SPEAR total block score from baseline to peak stress (D3 minus D1) were calculated to assess change in military tactical adaptive decision making and groups were stratified based on increase (high adaptors) or decrease (low adaptors) of the SPEAR change score. Overall, military tactical decision-making declined 1.7% from D1 to D3 (p < 0.001). High adaptors reported significantly higher scores of aerobic capacity (p < 0.001), self-report resilience (p = 0.020), extroversion (p < 0.001), and conscientiousness (p < 0.001). at baseline compared to low adaptors, while low adaptors reported greater scores in Neuroticism (p < 0.001). The present findings suggest that service members whose adaptive decision making abilities improved throughout SMOS (i.e., high adaptors) demonstrated better baseline psychological/self-reported resilience and aerobic capacity. Further, changes in adaptive decision-making were distinct from those of lower order cognitive functions throughout SMOS exposure. With the transition of future military conflicts placing higher priority on enhancing and sustaining cognitive readiness and resiliency, data presented here demonstrates the importance of measuring and categorizing baseline measures inherent to military personnel, in order to change and train one's ability to suffer less of a decline during high stress conditions.

Motion Tracking of Daily Living and Physical Activities in Health Care: Systematic Review From Designers' Perspective.

Motion tracking technologies serve as crucial links between physical activities and health care insights, facilitating data acquisition essential for analyzing and intervening in physical activity. Yet, systematic methodologies for evaluating motion tracking data, especially concerning user activity recognition in health care applications, remain underreported. This study aims to systematically review motion tracking in daily living and physical activities, emphasizing the critical interaction among devices, users, and environments from a design perspective, and to analyze the process involved in health care application research. It intends to delineate the design and application intricacies in health care contexts, focusing on enhancing motion tracking data's accuracy and applicability for health monitoring and intervention strategies. Using a systematic review, this research scrutinized motion tracking data and their application in health care and wellness, examining studies from Scopus, Web of Science, EBSCO, and PubMed databases. The review used actor network theory and data-enabled design to understand the complex interplay between humans, devices, and environments within these applications. Out of 1501 initially identified studies, 54 (3.66%) were included for in-depth analysis. These articles predominantly used accelerometer and gyroscope sensors (n=43, 80%) to monitor and analyze motion, demonstrating a strong preference for these technologies in capturing both dynamic and static activities. While incorporating portable devices (n=11, 20%) and multisensor configurations (n=16, 30%), the application of sensors across the body (n=15, 28%) and within physical spaces (n=17, 31%) highlights the diverse applications of motion tracking technologies in health care research. This diversity reflects the application's alignment with activity types ranging from daily movements to specialized scenarios. The results also reveal a diverse participant pool, including the general public, athletes, and specialized groups, with a focus on healthy individuals (n=31, 57%) and athletes (n=14, 26%). Despite this extensive application range, the focus primarily on laboratory-based studies (n=39, 72%) aimed at professional uses, such as precise activity identification and joint functionality assessment, emphasizes a significant challenge in translating findings from controlled environments to the dynamic conditions of everyday physical activities. This study's comprehensive investigation of motion tracking technology in health care research reveals a significant gap between the methods used for data collection and their practical application in real-world scenarios. It proposes an innovative approach that includes designers in the research process, emphasizing the importance of incorporating data-enabled design framework. This ensures that motion data collection is aligned with the dynamic and varied nature of daily living and physical activities. Such integration is crucial for developing health applications that are accessible, intuitive, and tailored to meet diverse user needs. By leveraging a multidisciplinary approach that combines design, engineering, and health sciences, the research opens new pathways for enhancing the usability and effectiveness of health technologies.

A review of evidence-based management of infectious ocular surface disease in shelter-housed domestic cats.

Infectious ocular surface disease (IOSD) is a common problem in shelter-housed domestic cats and has a widespread negative impact on animal welfare. While the common etiological agents are well-described, addressing IOSD in large groups of animals presents a management challenge to the clinician and logistical challenges to shelter employees. Treatments, diagnostics, and prevention strategies that are effective in privately owned or experimental animals may be impractical or ineffective in the shelter environment. This review article focuses on the relative prevalence of etiological agents in feline IOSD, practical diagnostic testing protocols, prevention strategies, and treatment of IOSD in shelter-housed cats. Discrepancies between experimental laboratory-based studies and clinical trials assessing therapeutics for treatment of feline herpes virus are highlighted. Further high-quality clinical trials are necessary to determine optimal preventative and therapeutic protocols for IOSD in shelter-housed cats.

The Use of Toothpastes Containing Different Formulations of Fluoride and Bioglass on Bleached Enamel

To investigate the application of toothpaste either containing calcium sodium phospho-silicate bioglass (NovaMin) or calcium fluorosilicate bioglass (BioMinF) on the surface mineral composition and morphology of enamel after bleaching procedure. Methods: Thirty extracted noncarious human teeth were allocated into five groups (n = 6). Group 1: Bleaching using 40% hydrogen peroxide (HP) and fluoridated toothpaste containing bioactive glass (1450 ppm fluoride). Group 2: Bleaching using 40%HP and toothpaste containing calcium fluorosilicate bioglass (540 ppm fluoride). Group 3: Bleaching using 40%HP and fluoridated toothpaste (1450 ppm fluoride). Group 4: Bleaching alone using 40%HP. Group 5: Negative control with distilled water alone. The surface morphology was evaluated using Scanning Electron Microscope (SEM) and Scanning Probe Microscope (SPM). The concentration of elements as atomic percentages were determined by X-ray Photoelectron Spectroscopy (XPS) and Energy-Dispersive X-ray Spectroscopy (EDS). Results: This laboratory-based study reported that SPM and SEM detected minor changes on the surfaces of all toothpaste-treated enamel samples (Groups 1-3) after 45 days. Bioactive glass deposits were observed on enamel surfaces in Groups 1 and 2, whilst the bleaching-alone samples (Group 4) had rough enamel surfaces. XPS reported that toothpaste containing calcium fluorosilicate bioglass (Group 2) had a high atomic% of calcium and phosphate, whilst silicon values were high in the toothpaste containing bioactive glass and 1450 ppm fluoride (Group 1) after bleaching procedure when compared to other groups (p < 0.05). In addition, EDS detected the highest %F in Groups 1, 2 and 5. Conclusions: Within the limitations of this laboratory-based study, there was no significant decrease in the Ca%, P% values and surface properties of enamel after the bleaching procedure following the use of different formulations of toothpastes for a period of 45 days. However, the Ca% and P% values were significantly high for the toothpaste containing calcium fluorosilicate bioglass (BioMinF) on the bleached enamel. Clinical relevance: The bleaching process can provide optimum aesthetic outcomes, but the effect of peroxides on hard tissues is still in question. Toothpastes containing different formulations of fluoride and bioactive glass might have the potential to prevent mineral loss on bleached enamel. However, further laboratory-based studies and controlled double-blind randomised clinical trials are required to interpret the effects of toothpastes with different fluoride and bioactive glass formulations on enamel surfaces following bleaching procedures.

Laboratory-based measures of temperature preference and metabolic thermal sensitivity provide insight into the habitat utilisation of juvenile California horn shark (Heterodontus francisci) and leopard shark (Triakis semifasciata).

Laboratory-based studies examining fish physiological and behavioural responses to temperature can provide important insight into species-specific habitat preferences and utilisation, and are especially useful in examining vulnerable life stages that are difficult to study in the wild. This study couples shuttle box behavioural experiments with respirometry trials to determine the temperature preferences and metabolic thermal sensitivity of juvenile California horn shark (Heterodontus francisci) and leopard shark (Triakis semifasciata). As juveniles, these two species often occupy similar estuarine habitats but display contrasting behaviours and activity levels - H. francisci are relatively sedentary, whereas T. semifasciata are more active and mobile. This study shows that juvenile H. francisci and T. semifasciata have comparable thermal preferences and occupy similar temperature ranges, but H. francisci metabolism is more sensitive to acute changes in temperature as expressed through a higher Q10 (H. francisci=2.58; T. semifasciata=1.97; temperature range: 12-24°C). Underlying chronic temperature acclimation to both warm (21°C) and cool (15°C) representative seasonal temperatures did not appear to significantly affect these parameters. These results are discussed in the context of field studies examining known distributions, habitat and movement patterns of H. francisci and T. semifasciata to better understand the role of temperature in species-specific behaviour. Juvenile H. francisci likely target thermally stable environments, such as estuaries that are close to their preferred temperature, whereas juvenile T. semifasciata metabolism and behaviour appear less dependent on temperature.

Flower symmetry in Saxifraga revisited

Monosymmetry in flowers has evolved many times from ancestors with polysymmetrical flowers. Flower symmetry characteristics often lack intraspecific variability. The genus Saxifraga represents an exception with high variations of floral symmetry between and within species and even within individual plants. Lab-based studies could show that this variation is to some extent caused by the flowers’ response to gravity and thus varies regularly between horizontally and vertically oriented flowers. Here, we investigate inter- and intra-individual variation in flower size, flower colouration, and floral symmetry in three species, Saxifraga stellaris, S. rotundifolia, and S. paniculata. In all three species, the stamen that initiates the sequential stamen movement is mostly downward pointing. In S. stellaris and S. paniculata, the terminal flowers are larger than the other flowers. In S. stellaris and S. rotundifolia, the size of floral guides is larger and the colour is stronger on the upward pointing 3 petals of diagonally and vertically oriented flowers, whereas the other two petals have paler or smaller floral guides. The septum between the two carpels is mostly oriented horizontally. We discuss the evolution of flower monosymmetry in regard to the morphological symmetry referring to symmetry defined by the mirror plane between carpels, petals and moving stamens, and visual symmetry defined by the pollinators view on the floral colour pattern and visible morphological features.