Laboratory Analysis Research Articles

Rotational Thromboelastometric Profile in Early Sepsis: A Prospective Cohort Study.

Coagulation abnormalities are common in sepsis patients and are associated with increased mortality. This study aimed to assess the hemostatic profile of sepsis patients using rotational thromboelastometry (ROTEM) and to find the ROTEM parameters best predicting short-term mortality. We conducted a prospective analysis of consecutive sepsis patients hospitalized in the intensive care unit. The inclusion criteria were diagnosis of sepsis or septic shock and pro-calcitonin concentration >0.5 ng mL-1. Clinical, standard laboratory, and ROTEM analyses were performed. The study group comprised 38 (49%) males and 40 (51%) females. Median Sequential Organ Failure Assessment (SOFA) score was 8 (interquartile range IQR 5-11) points. The most common primary sites of infection were pneumonia (n = 27/35%), intra-abdominal (n = 27/35%), urinary tract infection (n=20/26%), and others (n = 4/6%). The following parameters evaluating fibrinogen function were outside the reference range: clotting time (CT), clot amplitude (A) at 10 and 20 min, and maximal clot firmness (MCF). Out of 78 patients, 28 (36%) died in the intensive care unit. Significant differences between survivors and non-survivors of sepsis were present for the ROTEM parameters assessing fibrinolytic activity. ROTEM in the early phase of sepsis reveals increased coagulation mediated through the function of fibrinogen. Non-survivors showed slightly lower fibrinolytic activity than survivors; however, it was still within test reference values. The highest predicting value was obtained by a model incorporating, among others, extrinsic coagulation pathway fibrinolytic parameters.

Targeted quantification of per- and polyfluoroalkyl substances (PFASs) in livestock serum by liquid chromatography-high-resolution mass spectrometry.

Per- and polyfluoroalkyl substances (PFASs) have attracted increasing attention due to their persistence in the environment and potential adverse effects on human and animal health. The detection and quantification of PFASs in livestock could substantially contribute to monitoring their presence within the food chain. We developed a targeted quantification method for 34 PFASs in livestock serum by liquid chromatography-high-resolution mass spectrometry (LC-HRMS). We used protein precipitation for serum sample extraction and accurate mass measurement of targeted PFAS compounds for quantification. We validated the method with various analytical parameters, achieving accuracy of 70-120% and precision of <20%. The method also demonstrated good analytical sensitivity, with a limit of detection of <0.051 ng/mL and a limit of quantification of <0.175 ng/mL. When applying the developed method to actual serum samples from a variety of livestock, we successfully identified and quantified various PFASs in different livestock species. Our method has the potential to be a valuable tool for veterinary laboratory analysis of PFAS contamination in livestock.

Analysis of Clean Water Quality and Distribution in the Salate Area (RT 13/RW 03) of Passo Village, Baguala District, Ambon City

This study aims to analyze the quality of clean water and the distribution of clean water in the Shooting Range Complex (SalatE) RT 13 RW 03 Negeri Passo, Baguala District, Ambon City. This research uses a qualitative descriptive approach with a data collection period from August 21 to September 21, 2023. The method of taking physical samples was carried out on three water units representing water sources in the complex: distribution pipes from springs dug wells, and borehole water. Meanwhile, social aspect samples were taken from community members who own dug wells, bore wells, and those who use village government-owned water. In this study, samples were selected representatively from the population, namely 23 households (KK) out of 175 KK in the complex. The samples comprised 2 KK owners of dug wells, 8 KK owners of bore wells, and 12 KK users of village government-owned water. Data were obtained through observation, interviews, documents, and questionnaires. Data analysis was conducted based on the results of Laboratory Analysis. The results showed that the availability of clean water has yet to meet the needs optimally, both in terms of quality and quantity. In terms of quality, there is an indication that spring water in Desa Passo approaches the maximum requirements for clean water according to health regulations, especially in the Total Coliform parameter. It indicates the need for more attention to monitoring and managing water quality and improving infrastructure to ensure a safe and adequate supply of clean water for the people of Desa Passo.

One-year results of the first road surface with the addition of sunflower oil porous capsules

This paper presents findings from the first surface course in the UK employing sunflower oil-filled porous capsules as a self-healing additive. The capsules, designed to mitigate ravelling, were tested on a road constructed at an asphalt plant and subjected to a year of traffic in temperatures ranging from below 0°C to 35°C. Spherical in shape with a diameter of about 1.5 mm, the capsules possess a porous structure filled with sunflower oil. Each tonne of asphalt contained 5 kgs of capsules. The material used was Stone Mastic Asphalt (SMA), one of the UK's most utilised surface course materials. Eight sections were built, with four varying binder contents (6.4 %, 5.9 %, 5.5 % and 5.2 %), each with and without capsules. The road performance was evaluated by measuring two indicators: rut depth and surface macrotexture. Results show that the capsules delay fretting progression in wearing courses. Fretting could have resulted from the complex interaction between early life wearing and asphalt compaction by traffic that caused the mastic to move to the road surface. In addition, no considerable difference in rutting was observed between the different materials. Moreover, core samples were extracted from the road before and after traffic and age exposure for further laboratory analysis to assess the long-term performance. Results showed that stone loss resistance in asphalt-containing capsules was 30.02 % lower before ageing and 25.92 % lower after ageing. Furthermore, the stiffness ageing factor was 15–20 % lower in materials with capsules, while the fatigue life remained consistent across all materials.

Grain textural bias in detrital single-mineral provenance studies

Detrital single-mineral geochemistry and geochronology are strong tools in provenance studies and indicate great potentials in addressing issues in earth sciences. Various biases (both natural and artificial) exist objectively and may mislead provenance interpretations. Both the sedimentary sorting process and hand-picking in-laboratory processing may lead to analyzed grain textural (e.g., size and shape) variability and thus may introduce biases in single-mineral provenance analysis. Here, we take the Mesozoic–Cenozoic Qaidam basin, northeastern Tibet, as an example to investigate the relationship between single-mineral grain texture and detrital zircon geochronological and detrital tourmaline, rutile and garnet geochemical data and to explain how grain texture affects detrital single-mineral provenance interpretations. Results indicate that Precambrian zircons take less proportions in coarse (>125 μm), subrounded and high aspect ratio (>2) fractions than Phanerozoic zircons. Parent rock lithology discrimination results of detrital tourmaline and garnet in different grain size fractions show significant differences. Zr-temperature values of detrital rutile have an increasing trend with increasing grain size. The geochemistry of detrital tourmaline, rutile and garnet shows no dependence with grain aspect ratio and roundness. We suggest that inheritance of grain texture features from parent rocks is the major reason. Detrital zircons from recycled (meta)sedimentary rocks tend to be smaller and more rounded than those from igneous rocks. Detrital tourmaline, rutile and garnet grains from different parent rock types vary in size. Grain textural bias may cause the underestimated contributions of the Qilian Shan to the Cenozoic Qaidam basin if small detrital zircons were not involved in the analysis. Quantitative description of the source-to-sink system of the Cenozoic Qaidam is also influenced by grain textural bias. This study highlights the underestimated grain textural bias in single-mineral provenance studies. We suggest that a comprehensive understanding of potential sedimentary sources, depositional processes, sample petrographic features and laboratory analysis procedures is important to reliable provenance interpretations and to related implications in earth sciences.

An Insight into Practices Associated with the Control of Internal Parasites in the Dairy Goat Herds of Romania: A Questionnaire Survey.

The widespread and uncontrolled use of anthelmintic products has contributed to the emergence of anthelmintic resistance (AR). This phenomenon globally threatens the productivity and welfare of small ruminants. A questionnaire consisting of 34 questions was handed to 234 goat farmers across Romania to gain insight into control practices against internal parasites and the farmers' perception of the parasitic infections present in their herds and the efficacy of anthelmintic treatments. The majority of farmers (88.5%) admitted they had never submitted fecal samples for parasitological laboratory analysis, and 77.4% had treated the animals on their own. In general, the farmers dewormed their goats based on visual body weight estimation. Prophylactic anthelmintic treatment was practiced by more than 85% of the farmers. A traditional control approach based on treating the entire herd at fixed time intervals is widespread among Romanian goat and sheep farmers. The most commonly used anthelmintic drugs in the previous 3 years (2021-2023) were benzimidazoles (85.5%) and macrocyclic lactones (81.6%). Poor anthelmintic efficacy was suspected by 14.5% of farmers, and the minority (18.0%) considered internal parasites as a problem in their herds. Regarding the farmers' perception of the presence of parasites, there was a significant level of uncertainty. This is the first survey carried out in Romanian goat herds, and it provides up-to-date information on practices aimed at controlling internal parasites.

The effects of drone transportation on routine laboratory, immunohematology, flow cytometry and molecular analyses.

Transportation of medical samples between laboratories or hospital sites is typically performed by motorized ground transport. Due to the increased traffic congestions in urban environments, drone transportation has become an attractive alternative for fast shipping of samples. In accordance with the CLSI guidelines and the ISO 15189 standard, the impact of this transportation type on sample integrity and performance of laboratory tests must be thoroughly validated. Blood samples from 36 healthy volunteers and bacterial spiked urine samples were subjected to a 20-40 min drone flight before they were analyzed and compared with their counterparts that stayed on the ground. Effects on stability of 30 routine biochemical and hematological parameters, immunohematology tests and flow cytometry and molecular tests were evaluated. No clinically relevant effects on blood group typing, flow cytometry lymphocyte subset testing and on the stability of the multicopy opacity-associated proteins (Opa) genes inbacterial DNA nor on the number of Abelson murine leukemia viral oncogene homolog 1 (abl) housekeeping genesin human peripheral blood cells were seen. Forthreeofthe 30 biochemistry and hematology parameters a statistically significant difference was found: gamma-glutamyl transferase (gamma-GT), mean corpuscular hemoglobin (MCH) and thrombocyte count. A clinically relevant effect however was only seen for potassium and lactate dehydrogenase (LDH). Multi-rotor drone transportation can be used for medical sample transportation with no effect on the majority of the tested parameters, including flow cytometry and molecular analyses, with the exception of a limited clinical impact on potassium and LDH.

Considerations for laboratory analysis and interpretation of results

Considerations for laboratory analysis and interpretation of results

Modeling of Soil Cation Exchange Capacity Based on Chemometrics, Various Spectral Transformations, and Multivariate Approaches in Some Soils of Arid Zones

Cation exchange capacity is a crucial metric for managing soil fertility and promoting agricultural sustainability. An alternative technique for the non-destructive assessment of important soil parameters is reflectance spectroscopy. The main focus of this paper is on how to analyze and predict the content of various soil cation exchange capacities (CEC) in arid conditions (Sohag governorate, Egypt) at a low cost using laboratory analysis of CEC, visible near-infrared and shortwave infrared (Vis-NIR) spectroscopy, partial least-squares regression (PLSR), and Ordinary Kriging (OK). Utilizing reflectance spectroscopy with a spectral resolution of 10 nm and laboratory studies with a spectral range of 350 to 2500 nm, 104 surface soil samples were collected to a depth of 30 cm in the Sohag governorate, Egypt (which is part of the dry region of North Africa), in order to accomplish this goal. The association between the spectroradiometer and CEC averaged values was modeled using PLSR in order to map the predicted value using Ordinary Kriging (OK). Thirty-one soil samples were selected for validation. The predictive validity of the cross-validated models was evaluated using the coefficient of determination (R2), root mean square error (RMSE), residual prediction deviation (RPD), and ratio of performance to interquartile distance (RPIQ). The results indicate that ten transformation methods yielded calibration models that met the study’s requirements, with R2 &gt; 0.6, RPQ &gt; 2.5, and RIQP &gt; 4.05. For evaluating CEC in Vis-NIR spectra, the most efficient transformation and calibration model was the reciprocal of Log R transformation (R2 = 0.98, RMSE = 0.40, RPD = 6.99, and RIQP = 9.22). This implies that combining the reciprocal of Log R with PLSR yields the optimal model for predicting CEC values. The CEC values were best fitted by four models: spherical, exponential, Gaussian, and circular. The methodology used here does offer a “quick”, inexpensive tool that can be broadly and quickly used, and it can be readily implemented again in comparable conditions in arid regions.

Functional Cyperus esculentus L. Cookies Enriched with the Probiotic Strain Lacticaseibacillus rhamnosus SL42.

This study presents for the first time functional cookies for diabetics made with 100% organic Cyperus esculentus L. flour, either plain or enhanced with 5% polyfloral honey syrup containing the probiotic strain Lacticaseibacillus rhamnosus SL42. The flour's chemical composition and rheological and functional properties were analyzed, and 33 diabetic and semi-naive panelists assessed the cookies' sensory properties. MRS-cys agar cultures and SEM analysis evaluated SL42 survival and adhesion capacity over 21 days at 25 °C. Results showed that the flour and its extracts are rich in polyphenols and flavonoids, indicating strong antioxidant and antibacterial properties. Both cookie types met international standards when compared to commercial cookies and had similar physical properties. Sensory evaluation on day 1 revealed higher quality indicators for honey syrup-enriched cookies, but after 15 days, control cookies were preferred. The CIE LAB analysis confirmed the dietetic flour's typical dark color, with honey syrup-enriched cookies being darker. Despite textural differences, both cookies maintained detectable crispness over storage. Honey syrup-enriched cookies effectively carried L. rhamnosus SL42, remaining viable at 6.43 Log CFU per cookie after 21 days and adhering to the cookie's surface, as confirmed by SEM analysis. Further research is recommended to better understand the therapeutic value of these cookies.

Acute hepatitis E virus infection presenting as meningo-encephalitis.

Acute hepatitis E infection (HEV), with its high incidence in Europe, should be considered as a differential diagnosis of acute viral hepatitis and can in some cases manifest with pronounced neurological symptoms. We report on a 33-year-old female patient with severe arthralgia, myalgia, headache and psychomotor deterioration. Laboratory analyses showed elevated transaminases without signs of cholestasis. Acute hepatitis E virus infection was detected in serum. She reported fatigue and dysesthesias not responsive to analgesics. Cerebrospinal fluid (CSF) analysis revealed an inflammatory syndrome. HEV RNA was detected in the CSF. The infection remained mild, but dysesthesias persisted. Eight weeks after the first admission, the symptoms worsened again. Complete and sustained remission was achieved following intravenous corticosteroid treatment. In patients with acute neurological symptoms and liver enzyme elevation, HEV infection should be considered. Neurologic symptoms such as fatigue, arthralgia, myalgia and dysesthesia along with psychomotor retardation should prompt CSF analysis.

Geomechanics and Geology of Marine Terraces of the Crotone Basin, Calabria (Italy)

This study investigates the geomechanical behavior of five terrace orders in the Crotone Basin. The purpose is to understand the physical–mechanical parameters of these terraces to determine whether rock or soil mechanics principles should be applied for stability analysis. Samples were collected from each terrace following an extensive field survey. Laboratory analyses were conducted to measure pulse velocities, uniaxial unconfined compressive strength, and compressive strength with truncated conical platens. The findings revealed key physical–mechanical parameters of the rocks, which are crucial for stability assessments. The Crotone Basin, known for its mineral resources such as hydrocarbons and rock salts, has been studied geologically since before the 1950s, but there is a lack of geomechanical data in existing literature. Therefore, the results presented here are novel and provide a basis for future studies on the instability of rocky slopes composed of similar soft rock types. These results will aid in accurate geological–geotechnical model reconstructions. While the findings can be applied to similar cases, it is important to note that each analysis site, despite showing similar phenomena, is unique and requires individual investigation.

Factors influencing foraminiferal assemblages in coastal sediments of the Cross River Estuary, Southeast Nigeria, Gulf of Guinea

Estuaries are crucial coastal ecosystems facing increased pressures from human activities. The Cross River Estuary in Nigeria is a vital tropical mesotidal estuary influenced by diverse anthropogenic and natural processes. This study investigated the distribution of foraminiferal assemblages in the estuary's sediments, alongside environmental parameters such as heavy metals, pH, depth, temperature, salinity, mean grain size, and dissolved oxygen. Field sampling involved collecting 55 bottom water and sediment samples, with laboratory analyses focusing on grain-size distribution, foraminiferal identification, and heavy metal concentrations. Results indicated that heavy metal concentrations, except for iron, were within permissible limits. A total of 287 benthic foraminiferal tests representing four species were identified. These were primarily agglutinated species, with Arenoparrella mexicana and Miliammina fusca being the most abundant. Foraminiferal distribution was influenced by sediment type, salinity, and pH, rather than heavy metal concentration. Principal Component Analysis (PCA) revealed weak relationships between foraminiferal species and environmental variables, indicating limited influence of mean grain size and heavy metals on foraminiferal distribution. The study highlights the adaptability of certain foraminiferal species to a wide range of environmental conditions and underscores the need for continuous monitoring of the estuarine ecosystem to assess the impacts of ongoing anthropogenic activities. The findings provide a baseline for future environmental assessments and the use of foraminifera as bioindicators in tropical estuarine environments.

Soil Development and Land Suitability for Cacao on Sandstone and Pumice Breccia in the Semilir Formation in Ponjong District, Gunungkidul Regency, Indonesia

The parent material of the Semilir Formation, which consists of breccia pumice and sandstone, was located in the villages of Sawahan and Umbulrejo, Ponjong district, and it influences the genesis and soil types. This study utilized survey and laboratory analysis methods. Two soil profiles were studied: the first profile was located in Sawahan Village with breccia pumice as the parent material, while the second profile was in Umbulrejo Village with sandstone as the parent material. The research results indicated that soils developed from breccia pumice had diagnostic horizons of umbric epipedon and argillic endopedon with an advanced soil development stage. Soils developed from sandstone had diagnostic horizons of umbric epipedon and cambic endopedon with a young soil development stage. This affects the chemical properties of the soil. Soils formed from pumice breccia tend to have base saturation of less than 50% with low exchangeable bases, while soils formed from sandstone have base saturation of &gt;50% with high exchangeable bases. Soils developed from breccia pumice and sandstone have soil classification as Typic Paleudults and Typic Dystrudepts. These soil types are suitable for cocoa plant cultivation; however, they require mechanical conservation through terrace construction and improved drainage by incorporating organic materials or compost into the soil, which can significantly enhance soil porosity, enabling better water retention and drainage.

Soil Development and Land Suitability for Cacao on Sandstone and Pumice Breccia in the Semilir Formation in Ponjong District, Gunungkidul Regency, Indonesia

The parent material of the Semilir Formation, which consists of breccia pumice and sandstone, was located in the villages of Sawahan and Umbulrejo, Ponjong district, and it influences the genesis and soil types. This study utilized survey and laboratory analysis methods. Two soil profiles were studied: the first profile was located in Sawahan Village with breccia pumice as the parent material, while the second profile was in Umbulrejo Village with sandstone as the parent material. The research results indicated that soils developed from breccia pumice had diagnostic horizons of umbric epipedon and argillic endopedon with an advanced soil development stage. Soils developed from sandstone had diagnostic horizons of umbric epipedon and cambic endopedon with a young soil development stage. This affects the chemical properties of the soil. Soils formed from pumice breccia tend to have base saturation of less than 50% with low exchangeable bases, while soils formed from sandstone have base saturation of &gt;50% with high exchangeable bases. Soils developed from breccia pumice and sandstone have soil classification as Typic Paleudults and Typic Dystrudepts. These soil types are suitable for cocoa plant cultivation; however, they require mechanical conservation through terrace construction and improved drainage by incorporating organic materials or compost into the soil, which can significantly enhance soil porosity, enabling better water retention and drainage.

Evidence-based food serving size labelling: Survey and laboratory analyses of consumer cooking spray usage.

Nutrition label serving sizes are determined primarily based on typical consumption when such data are available. However, such data are not available for certain foods such as spray cooking oil (cooking spray). Our study assessed cooking spray use by the United States (US) adults compared to the 0.25-s serving size used on US-sold cooking spray labels. Adults (n = 1041, aged 33 ± 16.7 years) completed a 13-question survey on cooking spray use and perceptions. In the survey, participants reported using cooking spray for 1.9 ± 0.9 s per use, and 42.3%-43.1% of participants reported being more likely to purchase products if they were labelled calorie- or fat-free. Next, 30 adults (aged 29.7 ± 11.0 years) completed a laboratory-based study which assessed cooking spray durations for seven cookware items. Spray times ranged from 1.0 ± 0.5 (smallest pan) to 2.5 ± 1.3 s (largest baking sheet), with 100% of sprays (210/210) exceeding the 0.25-s US serving size. Our results suggest that cooking spray serving size should be increased to 1 s to better reflect actual consumption, and this would have the added benefit of aligning better with cooking spray serving sizes in other developed countries (0.5-1.0 s). A 1-s serving size would also preclude cooking spray advertised as calorie- or fat-free, allowing consumers to make more informed choices on the dietary implications of using cooking spray.

Functional and structural assessment of an experimental section gap graded modified with sugarcane bagasse ash

Gap-graded aggregate combined with asphalt rubber presents a high-performance alternative for roads with heavy traffic loads, offering advantages over conventional mixtures in terms of permanent deformation (rutting), fatigue life, and texture. In this study, the conventional filler in the well-established mixture was substituted with sugarcane bagasse bottom ash (SCBA) at a proportion of 5% of the total mineral aggregates. The objective was to enhance the mechanical performance of the asphalt coating while ensuring proper disposal of this waste material. Compared to conventional filler, SCBA is less dense, has smaller dimensions, and exhibits greater roughness, thereby affecting the volumetric parameters of the Marshall mix design. Consequently, the volume of voids in mineral aggregates and voids filled with asphalt increased while maintaining the same volume of air voids (5.3%). Consequently, there was a notable increase in Marshall Stability (40%) and Indirect Tensile Test (22%) mechanical parameters. Following laboratory analysis, the modified mixtures were applied as asphalt coating on a high-traffic highway (BR-158). Field specimens revealed an 18% increase in the Resilience Modulus (4088 MPa; 3478 MPa). Additionally, its Flow Number exhibited a 73% increase (16,707; 9681), and its permanent deformation rate was 28% lower within 10,000 cycles in the dynamic creep test. This was further supported by an 11% reduction in permanent deformation rate in the Hamburg Wheel Tracking Device (HWTD) within 20,000 cycles (3.2 mm; 3.6 mm). In conclusion, the partial replacement of conventional filler with sugarcane bagasse ash within the established granulometric range has demonstrated technical feasibility both in laboratory and field settings.

Study on Rock Characteristics for Assessing the Hydraulic Erodibility of Sandstones in the Manahari River Section, Sub-Himalaya, Central Nepal

The long-term erosion of the bed rock is steered by the power of the stream of variable magnitude and frequency which would give us the idea about bed rock incision and its channel morphology. Large numbers of infrastructural development work such as roads, bridges are undergoing in the Manahari Area. Hence, hydraulic erosion of the rocks is always a topic of interest while carrying out these construction works. Therefore, the main aim of this study is to determine the hydraulic erodibility of the Siwalik rocks under the action of stream power. Erodibility of the rocks and the stream powers of the Manahari River were determined by extensive field survey and laboratory analysis of rock material properties. Rock mass strength, block particle size, discontinuity/inter-particle bond shear strength, the shape of materials units, and their orientation relative to the flow were assessed to determine erodibility of the rocks. The longitudinal and cross-sectional surveys were carried out to find out the hydraulic parameters to calculate the erosive power of the stream i.e., slope of the channel surface, hydraulic radius, and velocity. The erodibility index ranges from 22 to 198 on the basis of their rock mass properties whereas the stream power value ranges from 1 to 6 kW/m2. The value of the stream power obtained at the bankfull condition at different flow time intervals i.e., 10, 25, 50, and 100 years ranges from 5 to 25 kW/m2. With this range of stream power at different time interval flow, the Manahara River has the capacity to erode maximum of the sandstones present in the riverbed as all the values of the erodibility plot above the threshold line of erosion. However, the relation between the erodibility index and stream power at normal flow condition shows that the Siwalik sandstones of the study area are not erodible under the influence of the available stream power.

Optimized Random Forest Method for 3D Evaluation of Coalbed Methane Content Using Geophysical Logging Data.

Accurate evaluation of coalbed methane (CBM) content is crucial for effective exploration and development. Traditional gas content measurement methods based on laboratory analysis of drill core samples are costly, whereas geophysical logging methods offer a cost-effective alternative by providing continuous high-resolution profiles of rock layer physical properties. However, the relationship between CBM content and geophysical logging data is complex and nonlinear, necessitating an advanced prediction method. This study focuses on the No. 3 coal seam in the Shizhuang South Block of the Qinshui Basin, utilizing geophysical logging data and 148 sets of laboratory core samples. We employed the Random Forest (RF) method optimized with a simulated annealing-genetic algorithm (SA-GA) to develop the SA-GA-RF model for evaluating CBM content. The model's performance was validated using test data and new CBM well data, and it was applied to calculate the vertical gas content profiles of No. 3 coal seam across 128 wells. The SA-GA-RF model demonstrated an average relative error of 13.13% in the test data set, outperforming Backpropagation Neural Network (BPNN), Least Squares Support Vector Machine (LSSVM), Extreme Learning Machine (ELM), and multivariate regression (MR) methods. The model also exhibited strong generalizability in new wells and improved model-building efficiency compared to traditional cross-validation grid search methods. The construction of a three-dimensional CBM content model, incorporating well coordinates and elevation data, allowed for detailed identification of high gas content areas and layers. This three-dimensional model offers a more precise characterization than traditional two-dimensional isopleth maps, providing valuable insights for CBM exploration, reserve evaluation, and production optimization.

Study of Serum Creatinine in Female Type 2 Diabetes Mellitus Patients

Diabetes mellitus is a chronic illness that affects millions of individuals worldwide. In the present study serum creatinine was estimated in female type 2 diabetes to observe the frequency of increase serum creatinine level in these patients. A cross-sectional, descriptive study was carried out in the Department of Physiology, Mymensingh Medical College, Bangladesh, between July 2016 and June 2017. A total of 210 subjects were selected and were grouped as: control group (group I) having 70 healthy female of reproductive age (21-45 years) and study group (group II), which was again subdivided to group IIA (70 diabetic female of reproductive age (21-45 years) and group IIB (70 diabetic female of post-menopausal age, 45-70 years). Laboratory analysis of serum creatinine was done by enzymatic colorimetric method. A comparison in serum creatinine concentration was done in between group I and group II and in between Group IIA and Group IIB. The results were calculated and analyzed by using SPSS. For statistical analysis students unpaired ‘t’ test was performed. Our study revealed that serum creatinine was increased among females of the study group and the result was statistically significant. In the control group serum creatinine was within the normal physiological range. CBMJ 2024 July: vol. 13 no. 02 P: 198-202