Labeled Sodium Acetate Research Articles

A study of carbon14 labeled acetate in Digitalis purpurea glycoside biosynthesis

A study has been made of the incorporation of acetate carboxyl carbon14 by Digitalis purpurea glycoside from labeled sodium acetate in hydroponic culture. A modified chromatographic method was employed for the isolation of the glycoside fractions. A major fraction, approximately 0.13 per cent yield, of amorphous glycoside was obtained, and this was found to be a glycoside of the gitoxin group. The chemical properties and radioactivity measurement of this glycoside fraction and its aglycone are reported.

THE BIOGENESIS OF ALKALOIDS: XIV. A STUDY OF THE BIOSYNTHESIS OF DAMASCENINE AND TRIGONELLINE

Tryptophan-3-C14was prepared from methyl labelled sodium acetate and fed to mature Nigella damascena L. plants. Radioactivity was translocated throughout the plant, but no activity was detected in the damascenine isolated from it. Similarly the radioactive tryptophan was fed to pea seedlings and trigonelline detected in the plant extracts. This was also inactive, and these results are discussed.

Über die Biosynthese des β‐Carotins bei Mucor hiemalis Wehmer. Die Beteiligung der Essigsäure am Aufbau der Carotinmolekel, insbesondere in den Jonongruppierungen, untersucht mit Hilfe von 14C‐markierter Essigsäure. II. Mitteilung

AbstractThe cleavage of radioactive β‐carotene, synthesized by the fungus Mucor hiemalis Wehmer from labeled sodium acetate (14CH3 · COONa and CH3 · 14COONa) with chromic acid leads to acetic acid. This acetic acid was decarboxylated by the Schmidt reaction. The radioactivity of methylamine and carbone dioxide (BaCO3) obtained from the Schmidt reaction has been examinated. These experiments indicate that (1) the methyl‐groups of the labeled acetic acid form the methylgroups of the chain between the two β‐jonone rings of the β‐carotene and the methyl‐groups in the position 5 and 5′ of the β‐jonone rings; (2) the carboxylic groups of the acetic acid on the other hand form the neighbouring C‐atoms of the methyl‐groups in the chain and the β‐jonone rings.