Development of logic AND-gated multivariate assay systems of DNA-scaffolded green- and red-fluorescent Ag nanoclusters (g-AgNC and r-AgNC) is promising and remains challenging. By using two DNA segments (To and Tr) as dual-targeting inputs, herein we report an innovative strategy that utilizes targets-recycled amplification to create a sensitive AND-gated light-up ratiometric sensor with reversely changed fluorescence of g-AgNC and r-AgNC. The design is based on that: two hairpins (Ho and Hr) are individually recognizable to To and Tr, and two hybridized duplexes are resultantly implemented for transient dissipative base pairing, i.e. Ho·Hr, releasing To and Tr for repeated recycling. In an original stem-loop hairpin reporter (HR), one exposed overhang is to host g-AgNC. When HR is unfolded by the cooperative sticky toeholds in Ho∙Hr, its liberated stem merging with the g-AgNC template is for r-AgNC clustering. Only when meeting simultaneously the overexpressed To and Tr in SARS-CoV-2, the AND-gated ratio fluorescence of r-AgNC over g-AgNC can be lighted-up in a typical assay, enabling highly specific-inputs binding and sensitive dose-dependence down to 0.29 pM. This enzyme-free and label-free AND-logic ratiometirc strategy illustrates the potential of using multicolor AgNCs emitters for more accurate multivariate assay.