Fibroblast Cell Line L929 Research Articles

Biofunctionalized bacterial cellulose membranes by cold plasmas

Bacterial cellulose (BC) membranes were modified with oxygen (O2), nitrogen (N2), and tetrafluoromethane (CF4) plasmas in order to enhance cell affinity. The surface properties of the pristine and plasma-treated BCs were analyzed through water contact angles, electron spectroscopy for chemical analysis (ESCA), and scanning electron microscopy. O2 and N2 plasmas changed the surface of BCs to more hydrophilic while CF4 plasma altered BCs to be very hydrophobic. ESCA analyses indicated that O2, N2, and CF4 plasmas incorporated the functionalities of carbon–oxygen, amides and amino, and carbon-fluoride on BCs, respectively. The effects of the plasma treatments on the adhesion of L-929 fibroblast and Chinese hamster ovary cell lines showed that the cell adhesion and proliferation of both cells was significantly improved on BC-CF4, in contrast with that on the pristine BC, BC-O2, and BC-N2, revealing a functionality-specific effect resulted from different plasmas. Moreover, protein adsorption tests indicated that a higher quantity of proteins in cell culture medium was adsorbed on the CF4 plasma-treated BCs which presumably played the role of enhancing the subsequent cell growth. This work highlights the great potential of plasma treatments on the improvement of biocompatibility and surface property of BCs for biomedical applications.

Transient Injury-Dependent Up-Regulation of CD105 and its Specific Targeting with an Anti-Vascular Anti-Mouse Endoglin-Nigrin b Immunotoxin

Endoglin (CD105), a cell-surface co-receptor for transforming growth factor-beta (TGF-β) superfamily members, is over-expressed in tumor neovasculature and can be targeted with anti-endoglin antibodies, thus becoming an important tool for anti-tumoral therapy. Injury of the mouse tail induced the transient expression of endoglin, this peaking at three days after injury and disappearing six days later. An immunotoxin containing the anti-mouse endoglin rat monoclonal antibody MJ7/18 and the non-toxic ribosome-inactivating protein nigrin b (Ngb) was found to be very active in targeting mouse endoglin in the L929 fibroblast cell line (IC(50) of 4 x 10(-11) M). At that concentration, the immunotoxin lacked unspecific activity. Upon induction of endoglin after injury, the MJ7-Ngb immunotoxin strongly attacked and deranged the injured tail, inducing tissue damage. Such effects were dependent on the age of the animals and were evident in six-week-old mice, but not in eight-month-old mice. Our results indicate that endoglin is up-regulated in newly formed vessels upon injury and can be targeted by the MJ7-Ngb immunotoxin; thus, it could be a useful tool for tumor ablation research.

Cytotoxicity evaluations of pristine graphene and carbon nanotubes in fibroblastic cells

This study concentrates upon investigating differential cellular responses to carbon nanoparticles (CNPs) such as pristine graphene flakes, single-walled carbon nanotubes (SWCNTs) and multiwalled CNTs (MWCNTs), in human dermal fibroblasts (HDFs) vs. the L-929 fibroblast cell line. Characterizing the surface morphology of each CNP by using scanning electron microscopy, we determined their cytotoxicity profiles by quantifying the mitochondrial activity. Graphene was found to have an irregular flake-like shape with various lengths within the ranges of 50 ∼ 1500 nm. The estimated sizes of the SWCNTs and the MWCNTs were about 400 nm and 1000 nm in diameter, respectively and several µm in length. All the CNPs tested here exerted adverse effects on the viability of HDFs even at 15.6 ppm, through intracellular uptake whereas except MWCNTs, they did not show any cytotoxicity against L-929 cells at 250 ppm. This result suggests that CNPs can have a differential influence on normal fibroblasts vs. immortalized ones.

Direct Condensation Reaction for Grafting of Polyethylene Glycol Monomethyl Ether on Poly(Methacrylic Acid-co-Methyl Methacrylate) for Application in Biomedical Engineering

Synthesis, characterization and cytotoxicity evaluation of copolymers based on polyethylene glycol monmethyl ether-g-poly(methacrylic acid-co-methyl methacrylate) are reported via a polymeric precursor method. Grafting was ac- complished based on direct condensation reaction in the presence of dicyclohexylcarbodiimide as an esterifica- tion-promoting agent catalyzed by dimethylamino pyridine. Polyethylene glycol grafted copolymers were characterized using various spectroscopic techniques; in addition, their biocompatibility was studied. Manifestation of bands assigned to the ester functional groups in Fourier transform infrared spectra and nuclear magnetic resonance was employed for structural characterization of the grafted copolymers. Performance of grafting reaction was guaranteed by determination of grafting efficacy. Cytotoxicity evaluations of the grafted copolymer using L929 fibroblast cell line elucidated acceptable biocom- patibility profile; consequently, the applicability of the copolymers is confirmed for biomedical applications.

The bactericidal and biocompatible characteristics of reinforced calcium phosphate cements

Infection remains a serious medical problem in orthopaedic surgery. Antibiotic administration can be available either systemically via the blood stream or locally, directly into the infected bone. One of the main limitations of antibiotic administration is the development of multi-antibiotic-resistant bacterial strains. In this study, we developed bactericidal calcium phosphate cements (CPC) by incorporation of different molecular weight chitosan and hydroxypropyltrimethyl ammonium chloride chitosan (HACC). Two standard strains, S. epidermidis (ATCC35984) and S. aureus (ATCC25923), and one clinical isolate, methicillin-resistant S. epidermidis (MRSE), were selected to evaluate the antibacterial activity of these bone cements. Our data showed that the CPC loaded with low molecular weight chitosan and HACC significantly inhibited the bacterial adhesion and biofilm formation. In addition, HACC-containing CPC has no cytotoxic effects on both mouse pluripotent C3H10T1/2cell line and a murine L929 fibroblast cell line. We propose that HACC-containing CPC represents a promising polymer-based bactericidal bone scaffold in controlling orthopaedic surgery-related infection.

Fluorescent analogs of the marine natural product psammaplin A: synthesis and biological activity

The symmetrical disulfide psammaplin A from the marine sponge Pseudoceratina sp. was synthesized and structurally altered by replacement of one of the α-(hydroxyimino)acyl units by a fluorescent 4-coumarinacetyl moiety. Thus, the first fluorescent analogs of psammaplin A were obtained. Structural variation also covered the substitution pattern of the phenyl ring. Cytotoxicity of psammaplin A against the mouse fibroblast cell line L-929 (IC(50) 0.42 μg mL(-1)) was about two-fold higher than that of the fluorescent hybrid compounds, whereas the disulfide containing two 4-coumarinacetyl moieties was inactive. Fluorescence microscopy revealed uptake of the 4-coumarinacetyl-α-(hydroxyimino)acyl hybrids into the cytoplasm leading to fluorescence in close proximity of the nuclear envelope, most likely in the Golgi apparatus. We did not observe strong fluorescence inside the nucleus, where most of the target histone deacetylases are located. We conclude that reduction of the disulfide probably takes place outside the nucleus. The psammaplin-derived thiol exhibited potent activity against histone deacetylase in the low nanomolar range, but diminished cytotoxicity. Antimicrobial activity of the new derivatives was also determined.

Emulsion Electrospinning of a Collagen‐Like Protein/PLGA Fibrous Scaffold: Empirical Modeling and Preliminary Release Assessment of Encapsulated Protein

The effectiveness of a multifunctional scaffold produced by the electrospinning of emulsions composed of organic PLGA and aqueous collagen-like protein (denoted as Fol-8Col) solutions is demonstrated. The resultant Fol-8Col/PLGA fibrous scaffolds with homogeneous morphology have mean fiber diameters from 600 to 2,000 nm. A uniform distribution of encapsulated Fol-8Col in the fibers is observed by fluorescence microscopy. TEM is used to clarify the representative core/sheath structure of emulsion electrospun Fol-8Col/PLGA fibers. Preliminary release assessment of encapsulated Fol-8Col shows results of sustained release for more than one month from the Fol-8Col/PLGA fibrous mats. The cytocompatibility of fibroblast cell line L929 with the fibrous composite seems promosing.

Cytoprotective effect of γ-tocopherol against tumor necrosis factor α induced cell dysfunction in L929 cells

The antioxidant vitamin γ-tocopherol exerts protective and anti-inflammatory effects in various models of critical illness. The combination of actinomycin D and tumor necrosis factor α (TNFα) in the immortalized fibroblast cell line L929 is a well-established method to model pro-inflammatory cytotoxicity in cultured cells in vitro. The present study had two aims. First, we wished to characterize the contribution of reactive oxygen species (ROS) to the cell dysfunction and this commonly used model system of cell death. Second, we wished to investigate the effects of γ-tocopherol on this response. Cells were exposed to actinomycin D (0.5 µg/ml) + TNFα (100 pg/ml) in the absence or presence of 1 h of γ-tocopherol pre-treatment. The earliest change that was detected in our system in response to TNFα was an increase in mitochondrial oxidant production, already apparent at 45 min. Changes in glycolysis and oxidative phosphorylation parameters were already apparent at 2 h, as detected by the Seahorse Biosciences XF24 Flux Analyzer. By 6 h, a slight decrease in Cell Index was detected by impedance-based analysis, employing an electronic sensor array system (XCelligence). At the same time, a slight decrease in cell viability was detected by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) method, along with a significant increase in lactate dehydrogenase (LDH) release into the culture medium, and a detectable degree of mitochondrial membrane depolarization. Between 12 and 24 h, the cell viability (already at a low level) further declined, which coincided with a secondary, marked decline in the mitochondrial membrane potential. Pre-treatment of the cells with γ-tocopherol (10-300 µM) provided a significant protection against all of the functional alterations induced by actinomycin D and TNFα. The current study provides direct evidence that reactive oxidant formation plays an important role in the current experimental model of cell dysfunction, and demonstrates the protective effects of the potent endogenous antioxidant vitamin, γ-tocopherol. The mechanisms described in the current study may, in part, contribute to the protective effects of γ-tocopherol in various models of critical illness.

Synthesis, crystal structures and spectroscopy of meclofenamic acid and its metal complexes with manganese(II), copper(II), zinc(II) and cadmium(II). Antiproliferative and superoxide dismutase activity

Some new complexes of meclofenamic acid (N-(2,6-dichloro-m-tolyl)anthranilic acid), Hmeclo ( 1), with potentially interesting biological activities are described. Complexes [Mn(meclo) 2] ( 2), [Cu(meclo) 2(H 2O) 2] ( 3), [Zn(meclo) 2(H 2O) 2] ( 4) and [Cd(meclo) 2(H 2O) 2] ( 5) were prepared and structurally characterized by means of vibrational, electronic and 1H and 13C NMR spectroscopies. The crystal structure of complexes [Cu 4(meclo) 6(OH) 2(DMSO) 2] 2DMSO ( 3a) and [Cd(meclo) 2(DMSO) 3] ( 5a) have been determined by X-ray crystallography. Complex ( 3a) is a centrosymmetric tetramer built up around the planar cyclic Cu 2(OH) 2 unit. Complex 5a is mononuclear seven-coordinated complex with the meclofenamato ligand behaving as a bidentate deprotonated chelating ligand. Intra and intermolecular hydrogen bonds stabilize these two structures, while the crystal packing is determined by π–π and C−H−–π interactions. Meclofenamic acid and its metal complexes have been evaluated for antiproliferative activity in vitro against the cells of three human cancer cell lines, MCF-7 (breast cancer cell line), T24 (bladder cancer cell line), and A-549 (non-small cell lung carcinoma), and a mouse fibroblast L-929 cell line. Complex 5 exhibits the highest selectivity against MCF-7 and 4 shows the highest selectivity against T -24. Complexes 2– 5 were found to be more potent cytotoxic agents against T-24 and complex 5 against MCF-7 cancer cell lines than the prevalent benchmark metallodrug, cis-platin. The superoxide dismutase activity was measured by the Fridovich test which showed that complex [Cu(meclo) 2(H 2O) 2] is a good superoxide scavenger.

Synthesis, characterizations and biocompatibility of alternating block polyurethanes based on P3/4HB and PPG‐PEG‐PPG

Block copolymers with exactly controlled structures, that is, alternating block polyurethanes based on poly(3-hydroxybutyrate-co-4-hydroxybutrate) (P3/4HB-diol) and poly (propylene glycol)-poly(ethylene glycol)-poly(propylene glycol) (PPG-PEG-PPG) were synthesized by solution polymerization via specifically selective coupling reaction between terminal hydroxyl P3/4HB segment and isocyanate group end-capped PPG-PEG-PPG segment, using 1,6-hexamethylene diisocyanate (HDI) as end-capped agent. The chemical structure, molecular weight and distribution were systematically characterized by nuclear magnetic resonance spectrum (¹H NMR), Fourier transform infrared spectroscopy (FTIR) and gel permeation chromatography (GPC). The thermal property was investigated by differential scanning calorimetry (DSC) and thermogravimetric analysis. The hydrophilicity was studied by static contact angle of H₂O and CH₂I₂. DSC revealed that the PU3/4HB-alt-PPG-PEG-PPG exhibited a distinct change from amorphous to 30% crystallinity degree, T(g) from -25 to -50 °C, T(m) from 110 to 145 °C. The polyurethanes were more hydrophilic (water contact angle centers around 80 °) than the raw PHA materials. The platelet adhesion assay showed that the obtained polyurethanes had a lower platelet adhesion than the raw materials and the amount of platelet adhesion could be controlled by varying the segmental length of P3/4HB-diol. This could be explained by the inclusion of PPG-PEG-PPG between the P3/4HB segments, improving the hemocompatibility of P3/4HB. The cell culture assay revealed that the obtained polyurethanes were cell inert and unfavorable for the attachment of mouse fibroblast cell line L929 and rabbit blood vessel smooth muscle cells (RaSMCs). This suggests that these polyurethanes would be promising candidates as hemocompatibility and tissue-inert materials.

In vitro evaluation of antibacterial activity and cytotoxicity of novel nanocomposite material for bone filling

In this study, silver ion substituted nanohydroxyapatite, titania nanoparticles and polyamide 66 composite bone filling materials were prepared, which were expected to be a suitable bone repair material with good antibacterial activity, biocompatibility and osteoconductivity, for use in osteomyelitis surgery. The antibacterial activity and release of Ag ions in vitro were evaluated. Two types of bacteria strains, Staphylococcus aureus and Escherichia coli were used in this test. The results showed that the composite materials exhibited significant antibacterial effects on the two bacteria. Time course release testing of Ag ions from the composite materials on immersion in simulated body fluid showed pronounced Ag release in the first several days after immersion, and then the release speed slowed down after 14 days. International standard ISO 10993‐12:2007 was adopted for cytotoxicity evaluation using fibroblast cell line L929, and it was found that the cytotoxicities for the composite materials ranked are all within 1, which showed no cytotoxicity for the composite materials.

Cytotoxicity evaluation of a new fast set highly viscous conventional glass ionomer cement with L929 fibroblast cell line

Aim:This study aims to evaluate the cytotoxicity of a new fast set highly viscous conventional glass ionomer cement (GIC) with L929 fibroblasts.Materials and Methods:The cement capsule was mixed and introduced into a paraffin wax mould. After setting, the cement was incubated in Dulbecco's Modified Eagle's Medium. Six replicates of the material extract were added to the culture medium in 96-well plates. L929 mouse fibroblast cells were added into the wells and then incubated for 48 h. Dimethylthiazol diphenyltetrazolium bromide test was performed for cytotoxicity evaluation.Results:The results showed that this GIC brand did not yield a half-maximal inhibitory concentration value, IC50, as the cell viability was above 50% at all concentrations. Cell viability over 90% was observed at the concentrations of 3.125 and 1.5625 mg/ml. Maximum concentration of the material showed cell viability of 59.4%.Conclusions:This new fast set highly viscous conventional GIC showed low cytotoxicity to mouse fibroblast cells, and it can be suggested as a substitute for dental cements exhibiting a long setting time.

Novel mutants of human tumor necrosis factor with dominant-negative properties

Tumor necrosis factor (TNF) is a polyfunctional cytokine, one of the key mediators of inflammation and innate immunity. On the other hand, systemic or local TNF overexpression is typical of such pathological states as rheumatoid arthritis, psoriasis, Crohn's disease, septic shock, and multiple sclerosis. Neutralization of TNF activity has a marked curative effect for some diseases; therefore, the search for various TNF blockers is a promising field of protein engineering and biotechnology. According to the previously developed concept concerning the possibility of designing dominant-negative mutants, the following TNF variants have been studied: TNFY87H + A145R, TNFY87H + A96S + A145R, and TNFV91N + A145R. All of these form inactive TNF heterotrimers with the native protein. The ability of mutants to neutralize the effect of TNF was investigated. The addition of mutants to the native protein was shown to provide a concentration-dependent suppression of TNF cytotoxicity against the mouse fibroblast cell line L929. Thus, novel inhibitors of human TNF can be engineered on the basis of these muteins.

The evaluation of cytotoxicity of suab1 on l929 fibroblast cell line and the comparison with formocresol

Formocresol is common treatment in exposed pulp of primary teeth at caries, trauma and damaged with cavity preparation. Although, clinical and radiographic success and good clinical results are observed, dentistry societies are still searching for materials interchangeable with formocresol. Suab1 is a phytochemical agent that was selected because of its anti- bleeding effect. In this study, we evaluated cytotoxicity of SUAB1 in comparison with formocresol on L929 fibroblast cell line. Cytotoxicity evaluation performed with Crystal Violet Assey (ELISA), microscopic observation and optical counting. Results were calculated with Excel® software. Analysis of variance and t-test were used for statistical inference; p

Microstructure, mechanical properties, and in vitro biocompatibility of spark plasma sintered hydroxyapatite–aluminum oxide–carbon nanotube composite

In the present work, HA reinforced with Al 2O 3 and multiwalled carbon nanotubes (CNTs) is processed using spark plasma sintering (SPS). Vickers micro indentation and nanoindentation of the samples revealed contrary mechanical properties (hardness of 4.0, 6.1, and 4.4 GPa of HA, HA–Al 2O 3 and HA–Al 2O 3–CNT samples at bulk scale, while that of 8.0, 9.0, and 7.0 GPa respectively at nanoscale), owing to the difference in the interaction of the indenter with the material at two different length scales. The addition of Al 2O 3 reinforcement has been shown to enhance the indentation fracture toughness of HA matrix from 1.18 MPa m 1/2 to 2.07 MPa m 1/2. Further CNT reinforcement has increased the fracture toughness to 2.3 times (2.72 MPa m 1/2). In vitro biocompatibility of CNT reinforced HA–Al 2O 3 composite has been evaluated using MTT assay on mouse fibroblast L929 cell line. Cell adhesion and proliferation have been characterized using scanning electron microscopy (SEM), and have been quantified using UV spectrophotometer. The combination of cell viability data as well as microscopic observations of cultured surfaces suggests that SPS sintered HA–Al 2O 3–CNT composites exhibit the ability to promote cell adhesion and proliferation on their surface and prove to be promising new biocompatible materials.

Injection Molding of Titanium Alloy Implant For Biomedical Application Using Novel Binder System Based on Palm Oil Derivatives

Problem statement: Titanium alloy (Ti6Al4V) has been widely used as an implant for biomedical application. In this study, the implant had been fabricated using high technology of Powder Injection Molding (PIM) process due to the cost effective technique for producing small, complex and precision parts in high volume compared with conventional method through machining. Approach: Through PIM, the binder system is one of the most important criteria in order to successfully fabricate the implants. Even though, the binder system is a temporary, but failure in the selection and removal of the binder system will affect on the final properties of the sintered parts. Therefore, the binder system based on palm oil derivative which is palm stearin had been formulated and developed to replace the conventional binder system. Results: The rheological studies of the mixture between the powder and binders system had been determined properly in order to be successful during injection into injection molding machine. After molding, the binder held the particles in place. The binder system had to be removed completely through debinding step. During debinding step, solvent debinding and thermal pyrolysis had been used to remove completely of the binder system. The debound part was then sintered to give the required physical and mechanical properties. The in vitro biocompatibility also was tested using Neutral Red (NR) and mouse fibroblast cell lines L-929 for the direct contact assay. Conclusion: The results showed that the properties of the final sintered parts fulfill the Standard Metal Powder Industries Federation (MPIF) 35 for PIM parts except for tensile strength and elongation due to the formation of titanium carbide. The in vitro biocompatibility on the extraction using mouse fibroblast cell line L-929 by means of NR assays showed non toxic for the sintered specimen titanium alloy parts.

Chemico-physical modifications induced by plasma and ozone sterilizations on shape memory polyurethane foams

Thermally activated shape memory polyurethane foams are promising materials for minimally invasive surgical procedures. Understanding their physical and chemical properties, in vitro response and effects of sterilization is mandatory when evaluating their potential as biomaterials. In this work, we report on the characterization of two Cold Hibernated Elastic Memory (CHEM) foams before and after two novel low-temperature sterilization techniques (plasma and ozone). Foams have different transition temperatures (T(trans)), as determined by Tandelta peaks in DMA tests, that depend on their chemical composition: both foams possess excellent shape recovery ability (Recovery Rate up to 99%) in conventional shape recovery tests. Plasma sterilization (Sterrad sterilization system) resulted in a slight increase of open porosity, but no effects on bulk chemical and thermo-mechanical properties were observed. Ozone sterilization had a stronger effect on foams morphology, both in terms of an evident rupture of pore walls and surface oxidation. These modifications affected both thermomechanical and shape recovery behavior. Furthermore, plasma sterilized foams cytocompatibility was investigated with L929 fibroblast cell line in vitro, showing a good adhesion and proliferation, as confirmed by SEM observation and Alamar blue assay. The obtained results contribute to define the role of shape memory foams as biomaterials and open novel questions on the role of sterilization technique effects on cellular solids.

Cytotoxic evaluation of injectable cyclodextrin nanoparticles

Nanoparticles were prepared using beta-CDC6, which is an amphiphilic beta-cyclodextrin derivative modified on the secondary face with 6C aliphatic esters. A nanoprecipitation technique was used to prepare the blank nanoparticles without any surfactant and nanoparticles containing Pluronic F68 as surfactant in a concentration range of 0.1 to 1%. Nanoparticle formulations were characterized by particle size distribution and zeta potential measurements. Entrapment efficiency and in-vitro release profiles were determined and the cytotoxicity of these injectable nanospheres was evaluated against mouse fibroblast L929 cell line and human polymorphonuclear cells by methlythiazolyltetrazolium assay. As far as particle size and zeta potential are concerned, there is a relationship between surfactant presence and nanoparticle characteristics. However, these effects are not significant. It was also found that surfactant presence has no effect on model drug nimodipine encapsulation but accelerates the in-vitro release of the drug. Cell culture studies on mouse fibroblasts and human polymorphonuclear cells revealed a concentration-dependent cytotoxicity more pronounced in fibroblast cells. This led to the conclusion that the use of surfactants in injectable nanoparticles prepared from amphiphilic beta-cyclodextrins may lead to altered in-vitro properties and impaired safety for the drug delivery system.

Antibacterial effects of sol‐gel‐derived bioactive glass nanoparticle on aerobic bacteria

The aim of this work was to evaluate the antibacterial effect of bioactive glass nanopowders. The 58S, 63S, and 72S compositions were prepared via the sol-gel technique. Characterization techniques such as X-ray diffraction, transmission electron microscopy (TEM), Zetasizer, and X-ray fluorescent were used. The antibacterial activity was studied using Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Staphylococcus aureus. Cytotoxicity of the samples was evaluated using mouse fibroblast L929 cell line. The chemical compositions of the prepared samples were as predicted, and the particle size of the samples with an amorphous structure mainly ranged over 20-90 nm. At broth concentrations below 50 mg/mL, they showed no antibacterial activity. The 58S showed the highest antibacterial activity with the minimum bactericidal concentrations of 50 and 100 mg/mL for E. coli plus S. aureus and for P. aeruginosa, respectively. The 63S exhibited bactericidal and bacteriostatic effects on E. coli and S. aureus at concentrations of 100 and 50 mg/mL, respectively, at an minimum bactericidal concentrations of 100 mg/mL. However, 72S bioactive glass nanopowder showed no antibacterial effect. They showed no cytotoxicity. It was concluded that bioactive glass nanopowders could be considered as good candidates for the treatment of oral bone defects and root canal disinfection. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010.

Evaluation of the in vitro biocompatibility of orthodontic elastics

Aim: Latex has been extensively used in orthodontics since the advent of the specialty. Natural latex does not fall into the category of materials known to be entirely inoffensive. The objective of the present in vitro study is to test the hypothesis that there is no difference in the cytotoxicity between natural latex elastics of different colors. Methods: The present article compared different latex intra-oral elastics (5/16 = 7.9 mm, mean load). The samples were divided into six groups of 15 elastics according to their manufacturer: Groups N, Y, V, R, G and P (Uniden, natural latex elastics and colored elastics, namely, yellow, violet, red, green and pink, respectively). Cytotoxicity assays were performed by using cell culture medium containing cells from mouse fibroblast cell line L929. The cytotoxicity was evaluated by using the “dye-uptake” test, which was employed at two different moments (1 and 24 h). Data were compared by ANOVA and Tukey’s test (P 0.05) was found among the test elastics groups at 24 h. Conclusion: Latex elastics from natural, yellow, violet and red colors induced a lowest amount of cell lysis compared to the elastics green and pink colors at 1 h, all latex elastics were found to be highly cytotoxic, regardless of their color at 24 h.