L929 Cell Line Research Articles

Biosensor for integrin inhibition of mammalian cell adhesion and migration using micropatterned cell culture substrate and retroreflective optical signaling

Integrins are a family of transmembrane receptors that play a crucial role in cell adhesion and migration. Integrins can uniquely transduce biochemical signals bidirectionally across the membrane and physically link the cell-cell and cell-extracellular matrix (ECM) with ligand bonds. The arginyl-glycyl-aspartic acid (RGD) peptide motif is present in the ECM as a minimal recognition sequence for integrins. To leverage this property in cell-based therapy, RGD variants, such as cyclic-type RGDfK (c(RGDfK)), which share a similar structure with RGD but exhibit a higher affinity for integrins, have been developed. However, because most evaluation methods for newly developed RGD variants focus on affinity strength, tools for cellular effects are required. In this study, we developed a new platform that integrates micropatterned three-dimensional cell culture substrates with a non-spectroscopic optical analysis system to quantitatively analyze the effects of RGD variants on cell adhesion and migration. The specially micropatterned substrate provides a cell adhesive and migration area to provide a restricted analysis area. Owing to the characteristics of retroreflective Janus particles (RJPs), a non-spectroscopic optical analysis system provides long-term stable optical verification properties and a simple optical setup. These techniques were integrated to quantitatively determine the integrin inhibitory effect of various concentrations of RGD variant. To demonstrate the efficacy of the developed cellular level RGD variant testing platform, the model cell line L929 fibroblast and model RGD variant c(RGDfK) were analyzed ranging from 0 to 10 μM. The results showed that the developed system could effectively and quantitatively analyze the effects of RGD variants on cells across various concentrations.

Sublingual β-Glucan/Vitamin C-loaded nanoparticle-embedded polyethylene oxide nanofibrous mats

Nutritional supplements available in various forms provide the consumer with essential molecules demanded for well-being retention. Despite the conveniences provided by oral administration, significant disadvantages such as hepatic first-pass effect, limited absorption, and age-related swallowing problems have been recognized which should be addressed appropriately. Nano-sized biomaterials, which have recently gained popularity in numerous medical fields, have the potential to resolve these problems. In this study, a drug delivery system was designed for sublingual administration and fabricated as β-glucan/vitamin C-loaded chitosan/tripolyphosphate/polyvinylpyrrolidone (CS/TPP/PVP) nanoparticles (DNPs) embedded in polyethylene oxide (PEO) nanofibrous mats (DNFs). The optimum PNP size and DNP size for sublingual administration were obtained as 236 ± 1 nm and 257 ± 1 nm, respectively. The homogenous appearance of DNFs was demonstrated and measured as 783 ± 290 nm using scanning electron microscopy. The synthesized biomaterials were analyzed chemically, 3D-wisely, and thermally. The in vitro drug release kinetics investigation concluded that the sustained drug release of DNP over 15 days proceeded based on the first-order model, and 99.30 % of vitamin C and 99.70 % of β-glucan were released. Neither pure nor drug-loaded samples showed notable cytotoxicity in the 24-h 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay in the L929 cell line. Herein, it has been comprehended that DNF nutritional supplement can be a promising novel supplement dosage form with ease of use, high efficiency, and bioavailability than the conventional methods.

STRUCTURAL, MORPHOLOGICAL AND MAGNETIC PROPERTIES OF FUNCTIONALIZED MANGANESE IRON OXIDE NANOPARTICLES FOR BIOLOGICAL APPLICATIONS

The chitosan functionalized MnFe2O4 nanoparticles were synthesized using thermal decomposition method. The morphological, structural and magnetic properties of obtained chitosan functionalized magnetic nanoparticles were studied to assess the feasibility for biomedical applications. The crystallite size of nanoparticles observed using XRD was 15nm. Functional group attachment to surface of nanoparticles was analyzed using FTIR. Particle size of nanoparticles was studied by TEM analysis and it showed that particles were roughly spherical. Thermal stability and bonding strength of ligands to the surface of material was determined using TGA. The magnetization of chitosan functionalized nanoparticles observed by VSM is 56emu/g and VSM showed no coercivity and remanence existence, stipulating the super-paramagnetic behavior. The cell viability of chitosan functionalized nanoparticles was above 85% at concentration up to the 1.0mg mL-1 on mouse fibroblast cell line i.e. L929 cell line. These studies revealed that, MnFe2O4 nanoparticles are potential materials for biomedical applications.

Biocompatible and hydrophilic copper-complexed polyvinyl alcohol coating for antifogging surfaces

Visibility is decreased when fog accumulates on the clear surface of optical devices. It is quite desirable to design the surface with antifogging properties. The current study used a dip coating process to prepare copper-incorporated polyvinyl alcohol (PVA) hydrophilic coating on the hydroxylated glass substrate. The formation of the PVA-Cu complex by the hydroxyl group was determined by FTIR and XPS methods. The surface morphology, adhesion test, wettability behavior, and cytotoxicity were investigated by SEM, cross-cut tape, contact angle measurement, and MTT experiment. By varying the CuCl2 concentration (0.0125–0.0625 M), the durability and adhesion of the coating were improved, as evidenced by its minimal mass loss and water uptake. The optimum coating condition showed a thickness of about 24.0 ± 0.7 μm and a good optical transmittance of over 90 %. Below 0.0375 M, the coating stability and water resistance could not be achieved due to the weak complexation of Cu with the PVA coating. In contrast, the PVA-Cu3 coating (0.0375 M, Cu2+ ions) was identified as an optimized condition for improved antifogging performance due to the effective complexation of Cu with the PVA matrix while maintaining the hydrophilicity and wettability behavior. Furthermore, no cytotoxicity was observed by L929 cell lines in response to the prepared coating. The current study results revealed that the adhesive, hydrophilic PVA-Cux coating with a contact angle of less than 62° might demonstrate strong antifogging properties and find its usage for endoscopic applications.

Co-biopolymer of zein/starch hydrogel incorporated montmorillonite for efficient quercetin delivery in lung cancer treatment

Today, cancer treatment is an important issue in the medical world due to the challenges and side effects of ongoing treatment procedures. Targeted nanodrug delivery systems can replace current methods to mitigate such side effects. In this study, a novel nanocomposite of starch, zein, and MMT with the drug quercetin (QC) was synthesised with the aim of effectively killing A549 cancer cells despite reducing any harmful effects on normal cells, and then the effect of the nanosystem to evaluate the damages on L929 cell lines (healthy) and A549 (cancerous) were investigated. For this purpose, the drug release test from the nanosystem was conducted at two pH levels of 5.4 and 7.4, and it was observed that the nanosystem successfully released 29 % of the QC drug in the first 12 h. The prepared nanocomposites were analysed by XRD, FT-IR, FESEM, DLS, Zeta potential, and MTT techniques. It was found that the nanoparticles exhibited a consistent and even morphology, with an average particle size ranging from 50 to 100 nm. The DLS study provided evidence of the precise range of size and outstanding durability of the nanoparticles. The nanosystem exhibited a notable enhancement in the rate of cell death in malignant tumours, as evidenced by the MTT tests. The synthesised nanosystem showed a precise and pH-dependent release of the drug, which indicates the biocompatibility of the nanocarrier for the QC drug. According to this, it has the capacity to function as an acceptable substitute for the therapeutic techniques that are now being used.

Freezing and bioreactor in the low-concentration detergents: A novel approach in the decellularization of small-diameter arteries.

Using decellularized small-diameter vascular bypass substitutes (<6 mm) is an efficient method for bypass grafting. A solution containing 0.5% SDS (weight/volume) is commonly used for extended periods to generate acellular tissues. However, this solution causes damage to the microfibril structure and alters the mechanical forces. Hence, the objective of this study is to reduce the concentration of SDS to preserve the structure and achieve efficient decellularization. The study employs a diluted solution of 0.3% SDS (weight/volume) to treat fresh and frozen swine small-diameter arteries, utilizing physical methods such as freezing and thawing. The effectiveness of cell removal was evaluated using histological analysis and the remaining DNA content of the sample. Furthermore, the acellular circuit also assesses the cytotoxicity and proliferation of HUVECs to gauge their safety. Through the use of 0.3% SDS, a bioreactor system, and freezing-thawing, the pig arteries are successfully decellularized, resulting in residual DNA levels of less than 50 ng/mg dry weight. This process does not cause any major changes to the biomechanical or structural properties of the arteries. The acellular samples exhibit no toxicity on the L929 cell line and promote the growth of HUVECs at their highest rate on the fourth day. This allows for the placement of acellular vascular grafts to evaluate physiological processes within the animal body. This is an important requirement in clinical blood vessel transplantation.

Isolation of Orientia tsutsugamushi in Culture from Vellore, South India.

Background: Orientia tsutsugamushi, causative agent of scrub typhus is an obligate intracellular parasite. We present information on isolation of this pathogen at a tertiary care centre in Vellore, Southern India. Materials & Methods: PBMCs (peripheral blood mononuclear cells) collected from suspected scrub typhus patients were inoculated into Vero and L929 cell lines and incubated at 37°C with 5% CO2 for 30 days. They were examined for presence of Orientia tsutsugamushi on 10, 15, 20 days post-inoculation and everyday thereafter for a maximum of 30 days post inoculation. The scrapings were subjected to Giemsa staining, IFA, 47kDa qPCR and transmission electron microscopy (TEM). The isolates were passaged 3-4 times to ensure viability and then stored in DMEM with 10% FBS (-80). Genotyping of the isolates was performed by amplifying a 650 bp segment of the TSA 56 (type specific antigen 56) gene. Results: Amongst the 50 samples inoculated, three were culture positive as confirmed by 47 kDa qPCR on 24th day post inoculation. This was further confirmed by Giemsa, IFA staining and TEM. The 650bp amplicons showed 99.5 to 100% homology with Orientia tsutsugamushi MW604716, MH003839, MW604718, MW604717, MH922787 and MH003838 strains. Phylogenetic analysis revealed that 2 isolates belong to TA763 genotype and one belongs to Gilliam genotype. Conclusion: Orientia tsutsugamushi has been isolated for the first time at Vellore, South India from PBMCs. Complete genomic analysis will give more information.

Development and In vitro and In vivo efficacy investigation of multifunctional, targeted, theranostic liposomes for imaging and photodynamic therapy of glioblastoma

The challenge of effectively diagnosing and treating glioblastoma, which has a high incidence and low survival rate, remains unresolved. Consequently, research efforts have increased to develop specific and effective diagnostic and therapeutic agents. In this study, N-acetyl glucosamine-modified, IR780-encapsulated, PEG-coated, nanosized, 68Ga-labeled, neutral, and positively charged theranostic liposomes were developed for GLUT1 targeting for PET/CT and NIR imaging and PDT/PTT of GBM. Characterization, in vitro release profile, stability, radiolabeling efficiency, and labeling stability were evaluated. In vitro cell uptake studies were conducted in RG2 and U87 GBM cell lines, NIR images were obtained in the RG2 and U87 cell lines, and fluorescence microscope images were obtained in the RG2 cell line. PDT/PTT activities were assessed after exposure to NIR light (808 nm, 0.8–1 W/cm2). Cytotoxicity was studied in RG2, U87, and L929 cell lines. In vivo studies were performed in GBM model nude mice. The liposomes had an optimum mean particle size (181–193 nm), high encapsulation efficiency (79–84 %), and zeta potential (−5 to −7 mV). The maximum 68Ga labeling efficiency was achieved with an incubation at 80 °C, pH: 3.5, and 5 min. Liposomes remained stable for 30 days at 4 °C. While the intracellular uptake of targeted formulations was higher than non-targeted ones, it was similar for positively charged and neutral formulations. In U87 and RG2 cell lines, cell viability remained above 50 % for liposomal formulations at a concentration of 1.5 μg/mL exposed to NIR light (808 nm, 0.8–1 W/cm2). The formulations showed significant PDT/PTT activity under NIR light and lower cytotoxicity in the L929 than in RG2 and U87 cell lines. In vivo bioluminescence images and biodistribution studies in U87 tumor-bearing nude mice revealed higher tumor uptake of targeted, IR780-encapsulated liposomal formulations in tumor tissue compared to non-targeted ones. Liposomal formulations showed higher tumor uptake than the free IR780 solution. The data suggest that targeted liposomes have potential as theranostic agents for PDT/PTT and multifunctional glioblastoma imaging.

Antiviral respiratory masks with plasma-functionalized polypropylene textiles for optimal adsorption of antiviral substance

During the COVID-19 pandemic, face masks were the first line of defense against the spread of infection. However, infectious viruses may remain on medical textiles, potentially serving as an additional source of infection. Due to their chemical inertness, many textiles cannot be enhanced with antiviral functionalities. Through treatment with low-pressure gaseous plasma, we have activated the surface of a medical-grade melt-blown, non-woven polypropylene textile so that it can absorb sodium dodecyl sulfate, an antimicrobial surfactant. Within two hours of contact time, the functionalized textile has been able to inactivate over 7 log10 PFU mL−1 of bacteriophage phi6, a surrogate of enveloped viruses such as SARS-CoV-2, and it has retained its antiviral properties for over 100 days. The functionalized material has not disrupted facial mask filtration efficiency or breathability. In addition, the in vitro biocompatibility testing in accordance with ISO 10993-5 for testing of medical devices has demonstrated that the selected formulation causes no adverse effects on the mouse fibroblast cell line L-929. With the treatment processes that have been completed within seconds, the method seems to have great potential to produce antiviral textiles against future outbreaks.

Synthesis and characterization of a novel dual sensitive iron nanoparticles incorporated Schiff base composite hydrogel for diabetic wound healing therapy

Chronic wounds in diabetic patients deteriorate into multiple infections. A multifunctional transdermal material with high self-healing ability, remodeling capability, antibacterial and radicle scavenging activity, and an excellent multi-sensitive carrier was needed to promote wound healing. For that, Oxidized Cellulose (OC) and gelatin (GLN) are selected to construct a dual drug-loaded Schiff base hydrogel with iron nanoparticle (IONPS) incorporation for the controlled and sustained release of Insulin (INS) and Metfomin (MET), which synergistically promote wound repair. The INS/MET-IONPS-OC/GLN hydrogel drug payload was characterized using FT-IR, XRD, DLS, ZETA, TG, FE-SEM, TEM, and VSM analysis. The high drug loading and encapsulation efficiency were 93.20 % and 98.8 % for INS and 90.2 % and 95.1 % for MET, respectively. The temperature-sensitive drug release (92.0 % of INS and 90.0 % of MET) percentage is much better than the pH-sensitive drug release (83.5 % of INS and 80.2 % of MET). Above 90.0 % viability in MTT and apoptosis assay reveals the nontoxic nature of the INS/MET-IONPS-OC/GLN towards L929 normal cell lines. The zone of inhibition value of 12 and 15 mm in gram-negative bacteria reveals the anti-bacterial effect. The antioxidant activity of the carrier shields the cells against reactive oxygen species promotes healing rate ensures by DPPH assay.The cell proliferation and angiogenesis were confirmed by scratch assay on L929 cell lines in diabetic and non-diabetic conditions, showing the healing ability of the INS/MET-IONPS-OC/GLN hydrogel.

Cistus libanotis from Algeria: Phytochemical analysis by GC/MS, HS-SPME-GC/MS, LC–MS/MS and its anticancer activity

In this paper, Cistus libanotis (Cistus clusii Dunal) from Algeria was investigated. This plant is utilized in numerous industries, mostly in perfumery and, more recently, as a raw material for dietary supplements. For the first time, the present study evaluated the anticancer potential of Algerian C. libanotis by performing a comprehensive phytochemical investigation. The volatile fraction of the fresh plant material of C. libanotis was investigated using headspace solid-phase microextraction (HS-SPME) coupled to GC-MS, and the chemical composition of the essential oil (EO) of C. libanotis was examined using GC-MS technique; additionally, the phenolic compounds were identified and quantified using liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). A healthy cell line (L929) and two cancer cell lines (CAPAN-1 and dld-1) were used to test the ethanolic extract's cytotoxicity. HS-SPME-GC/MS analysis identified sabinene (25.3 %) and α-thujene (23.8 %) as principal components. In EO the major ingredients were γ-terpinene (17.4 %), camphene (16.13 %), α-phellandrene (15.36 %), and 4-carene (13.54 %). Nevertheless, 24 components were identified by LC-ESI-MS/MS analysis and shikimic acid, luteolin and hesperidin were most abundant. Further, the ethanolic extract of C. libanotis showed a significant cytotoxic effect against dld-1 and CAPAN-1 cell lines, while it was less active against the healthy cell line L929.

Micelles of poly[oligo(ethylene glycol) methacrylate] as delivery vehicles for zinc phthalocyanine photosensitizers

Drug-loaded polymeric micelles have proven to be highly effective carrier systems for the efficient delivery of hydrophobic photosensitizers (PSs) in photodynamic therapy (PDT). This study introduces the micellization potential of poly(oligoethylene glycol methyl ether methacrylate) (pOEGMA) as a novel approach, utilizing the hydrophobic methacrylate segments of pOEGMA to interact with highly hydrophobic zinc phthalocyanine (ZnPc), thereby forming a potential micellar drug carrier system. The ZnPc molecule was synthesized from phthalonitrile derivatives and its fluorescence, photodegradation, and singlet oxygen quantum yields were determined in various solvents. In solvents such as tetrahydrofuran, dimethyl sulfoxide, and N,N-dimethylformamide, the ZnPc compound exhibited the requisite photophysical and photochemical properties for PDT applications. The pOEGMA homopolymer was synthesized via reversible addition-fragmentation chain-transfer polymerization, while ZnPc-loaded pOEGMA micelles were prepared using the nanoprecipitation method. Characterization of the pOEGMA, ZnPc, and micelles was conducted using FTIR,1H-NMR, dynamic light scattering, matrix-assisted laser desorption/ionization time-of-flight mass spectrometries, gel permeation chromatography, and transmission electron microscopy. The critical micelle concentration was determined to be 0.027 mg ml-1using fluorescence spectrometry. The drug loading and encapsulation efficiencies of the ZnPc-loaded micelles were calculated to be 0.67% and 0.47%, respectively. Additionally, the release performance of ZnPc from pOEGMA micelles was monitored over a period of nearly 10 d, while the lyophilized micelles exhibited stability for 3 months. Lastly, the ZnPc-loaded micelles were more biocompatible than ZnPc on L929 cell line. The results suggest that the pOEGMA homopolymer possesses the capability to micellize through its methacrylate segments when interacting with highly hydrophobic molecules, presenting a promising avenue for enhancing the delivery efficiency of hydrophobic PSs in PDT. Moreover, it was also deciphered that obtained formulations were highly biocompatible according to cytotoxicity results and could be safely employed as drug delivery systems in further applications.

Green synthesis, characterization and biological activity analysis of silver nanoparticles from commercially available root powder of Picrorhiza kurroa Royle ex Benth.

Picrorhiza kurroa Royle ex Benth., a medicinal plant native to the alpine regions of the Himalayas, is renowned for its diverse therapeutic properties. Recently, green-synthesized silver nanoparticles have gained attention for their potential in pharmaceutical and industrial applications. In this study, silver nanoparticles (Pk-AgNPs) were synthesized using commercially available root powder of P. kurroa. The objective was to evaluate their potential biomedical applications by assessing their antioxidant properties and cytotoxicity. The Pk-AgNPs were characterized using Scanning Electron Microscopy (SEM) and Fourier-Transform Infrared Spectroscopy (FT-IR), which revealed their morphology and functional groups. The Total Antioxidant Capacity (TAC) of methanolic root extract of P. kurroa (PkRE) and the Pk-AgNPs was determined using the Phosphomolybdenum method. The results indicated that the antioxidant activity of PkRE was higher than that of the Pk-AgNPs. Moreover, the activity increased with concentration, ranging from 20 µg/mL to 120 µg/mL, demonstrating their capability to eliminate harmful free radicals. The cytotoxicity of PkRE and Pk-AgNPs was evaluated using the MTT assay against the SV40 T-antigen-containing HEK293T cell line, derived from human embryonic kidney 293 cells and the L929 cell line, a mouse fibroblast cell line. The results demonstrated dose-dependent cytotoxicity. L929 cells remained 100 % viable at concentration up to 200 µg/mL for PkRE and 100 µg/mL Pk-AgNPs. In contrast, HEK293T cells didn’t show 100 % viability even at a concentration of 50 µg/mL for both samples. This study highlights the potential use of PkRE and Pk-AgNPs as antioxidants. However, the cytotoxicity findings suggests that they may be harmful to humans if consumed orally, though they could be suitable for use in other organisms. This paves the way for further research into the application of AgNPs synthesized from P. kurroa in biomedical and pharmaceutical domains as well as in veterinary sciences.

A Minor Groove Binder with Significant Cytotoxicity on Human Lung Cancer Cells: The Potential of Hesperetin Functionalised Silver Nanoparticles.

Natural drug functionalised silver (Ag) nanoparticles (NPs) have gained significant interest in pharmacology related applications due to their therapeutic efficiency. We have synthesised silver nanoparticle using hesperetin as a reducing and capping agent. This work aims to discuss the relevance of the hesperetin functionalised silver nanoparticles (H-AgNPs) in the field of nano-medicine. The article primarily investigates the anticancer activity of H-AgNPs and then their interactions with calf thymus DNA (ctDNA) through spectroscopic and thermodynamic techniques. The green synthesised H-AgNPs are stable, spherical in shape and size of 10 ± 3nm average diameter. The complex formation of H-AgNPs with ctDNA was established by UV-Visible absorption, fluorescent dye displacement assay, isothermal calorimetry and viscosity measurements. The binding constants obtained from these experiments were consistently in the order of 104 Mol-1. The melting temperature analysis and FTIR measurements confirmed that the structural alterations of ctDNA by the presence of H-AgNPs are minimal. All the thermodynamic variables and the endothermic binding nature were acquired from ITC experiments. All these experimental outcomes reveal the formation of H-AgNPs-ctDNA complex, and the results consistently verify the minor groove binding mode of H-AgNPs. The binding constant and limit of detection of 1.8μM found from the interaction studies imply the DNA detection efficiency of H-AgNPs. The cytotoxicity of H-AgNPs against A549 and L929 cell lines were determined by in vitro MTT cell viability assay and lactate dehydrogenase (LDH) assay. The cell viability and LDH enzyme release are confirmed that the H-AgNPs has high anticancer activity. Moreover, the calculated LD50 value for H-AgNPs against lung cancer cells is 118.49µl/ml, which is a low value comparing with the value for fibroblast cells (269.35µl/ml). In short, the results of in vitro cytotoxicity assays revealed that the synthesised nanoparticles can be considered in applications related to cancer treatments. Also, we have found that, H-AgNPs is a minor groove binder, and having high DNA detection efficiency.

Development and characterization of a novel pH-Responsive nanocarrier for enhanced quercetin delivery and cytotoxicity in lung cancer

Quercetin (QC) is a potent polyphenol with numerous health benefits, but its use is limited due to its low bioavailability and high toxicity. Accordingly, a pH-responsive nanocarrier was created using chitosan (CS), halloysite nanotubes (HNTs), and graphene quantum dots (GQDs). It was found that LE and EE values of the CS/GQDs nanocomposites were 32.0% and 69.0%, respectively, increasing to 44.25% and 85.5% upon HNTs addition. The QC release study of pH-responsive CS/HNTs/GQDs nanocomposite in buffer environments showed that with the presence of GQDs in the CS/HNTs composite, the release rate increased from 73% and 54% at pH 5.4 and 7.4 to 97.5% and 67%. Examining the release data with kinetic models showed that the data corresponded to the first-order model, which indicates concentration-dependent drug release. In this study, the cytotoxicity of CS/HNT, CS/HNTs/GQDs, and CS/HNTs/GQDs@QC nanocomposites against L929 and A549 lung cell lines was investigated. The results showed that CS/HNTs/GQDs@QC had the highest cytotoxicity and had a more profound effect on A549 cells. The proportion of apoptotic cells in the CS/HNTs/GQDs@QC group was also higher than in the control group, with early and late apoptosis of 74.4% and 5.15%, respectively.

Influencing Factors and Stimulus-Sensitivity of Chitosan/Polymethacrylic Acid (CS/PMAA) Nanogels as Nanocarriers

ABSTRACT Nanogel, nanosized hydrogel, can contribute to biomedical and pharmaceutical, especially in drug delivery, due to its enthralling properties. Small size, large surface area, hydrophilicity, and stimulus responsiveness of nanogel ameliorate tissue penetration, drug loading capacity, and targeted drug delivery. The current study prepared nanosized, pH-sensitive, biocompatible, and stable chitosan/polymethacrylic acid (CS/PMAA) based nanogels using MBA as a crosslinker and APS as an initiator. FTIR confirmed the reaction between functional groups to synthesize nanogels sufficiently in a spherical shape, as affirmed by a FESEM. DLS data revealed nanosized particles with a size range of 70–170 nm and a range of 6–13 for polydispersity index (PDI). A study showed that 2% of MBA crosslinker is sufficient to achieve the desired crosslinking in 60 minutes reaction time (RT) with nanogel size ~ 92 nm. Increasing RT from 30 to 80 minutes during nanogel synthesis demonstrated a decrease in nanogel sizes from 186–73 nm. Synthesized nanogels have shown pH sensitivity (stimuli responsiveness) in acidic and alkaline mediums with an increase in particle size in pH-2 (~144 nm) and pH-8 (~280 nm) compared to intermediate pHs. The pH sensitivity of nanogels by changing the size per the suspended medium’s pH is crucial for prolonged systemic circulation and targeted drug delivery. Moreover, nanogels have shown good colloidal stability with zeta potential (ZP) +28 mv in acidic and −38 mv alkaline mediums. DSC and XRD results demonstrated thermal stability and amorphous morphology of nanogels, respectively. BET surface analysis revealed the surface area of nanogels in the range of 65–68 cm2/g with an average pore size of 28–32 nm. In-vitro cytotoxicity in the L-929 cell line by MTT assay established the cell viability (>70%) after 24 hours of incubation, substantiating the non-toxicity and biocompatibility of nanogels as nanocarriers for drug delivery.

Effect of halogens on 3-[4-(dimethylamino) phenyl]-1-phenylprop-2-en-1-ones: development of a new class of monoamine oxidase-B inhibitors

Five dimethylamino-based chalcone derivatives (AC) were synthesized and evaluated for their inhibition degree against monoamine oxidase (MAO) enzymes. All AC compounds showed better inhibitory activity against MAO-B than that against MAO-A. AC4 showed the highest inhibitory ability with an IC50 value of 0.020 µM, similar to that of a reference drug safinamide (IC50 = 0.019 µM) against MAO-B, followed by AC1 (IC50 = 0.068 µM) and AC3 (IC50 = 0.083 µM). Substituent -F in ring A (AC4) increased the MAO-B inhibition, followed by -H (AC1), -Br (AC3), and -Cl (AC2). The selectivity index (SI) value of AC4 was high (SI = 82.00) as well as other compounds (44.41 to 98.15). AC4 was found to be a reversible inhibitor as confirmed through analysis using the dialysis method. Interestingly, AC4 was observed to be a noncompetitive MAO-B inhibitor with a rare case and with Ki values of 0.011 ± 0.0036 µM. These experiments confirmed that AC4 is a reversible and potent selective inhibitor of MAO-B. Molecular docking experiments revealed that AC4 showed the highest inhibitory activity with a docking score (-9.510 kcal/mol). A study using molecular dynamics modeling revealed that the protein–ligand complex was more stable. It was observed that AC4 was non-cytotoxic in the study using L929 cell line. In conclusion, compound AC4 shows promise as a MAO-B inhibitor.

Application of decalcified bone matrix in Salmon bone for tibial defect repair in rat model.

The optimal preparation conditions of Salmon decalcified bone matrix (S-DBM) were explored, and the properties of S-DBM bone particles and bone powder were studied respectively. The therapeutic effect of S-DBM on tibial defect in female Sprague Dawley (SD) rats was preliminarily verified. This study assessed the structural and functional similarities of Salmon bone DBM (S-DBM). The biocompatibility assessment was conducted using both in vivo and in vitro experiments, establishing an animal model featuring tibial defects in rats and on the L929 cell line, respectively. The control group, bovine DBM (bDBM), was compared to the S-DBM-treated tibial defect rats. Imaging and histology were used to study implant material changes, defect healing, osteoinductive repair, and degradation. The findings of our study indicate that S-DBM exhibits favorable repairing effects on bone defects, along with desirable physicochemical characteristics, safety, and osteogenic activity. The S-DBM holds significant potential as a medical biomaterial for treating bone defects, effectively fulfilling the clinical demands for materials used in bone tissue repair engineering.

Synthesis, Evaluation and Docking Studies of Disubstituted N-Heterocyclic Derivatives as Anticancer Agents.

Cancer is a chronic disease reported with alarming rates of mortalities every year. Herein, we reported the synthesis of nitrogen based novel heterocyclic disubstituted derivatives and evaluated them against L929 and A549 cell lines using MTT assay. Among all, 6a2 and 6c1 were significantly active against L929 with IC50 value of 2.61±9.58 and 2.64±8.97 µg/mL respectively. Compounds 6a2 and 6c1 were also active against A549 with IC50 value of 2.36±9.20 and 2.43±6.28 µg/mL respectively and were found to be more potent than the standard drug Doxorubicin. A molecular docking study of the active compounds was also done against EGFR, conferring good binding affinity and binding interactions. Further biological investigations may provide valuable insights towards exploring the therapeutic potential of the active compounds in future.

Chitosan stabilized platinum nanoparticles: Synthesis, characterization and cytotoxic impacts on human breast cancer cells

Platinum nanoparticles are widely studied as a nanomedicine against many of the solid tumours. Due to their promising physicochemical properties, chitosan-stabilized platinum nanoparticles may exhibit exceptional cytotoxic effects on cancer cells. This article describes the synthesis and characterization of chitosan-stabilized platinum nanoparticles (Ch-Pt NPs) through a wet chemical method and in vitro studies of their anticancer effect on human breast cancer cells (MCF-7 cell line). Different analytical methods confirmed the formation of chitosan-stabilized platinum nanoparticles. The structural and surface morphological analyses were done using XRD, FTIR, TEM, FESEM, etc. Elemental analysis was done using XPS and EDX. The hydrodynamic diameter and zeta potential were determined using DLS and zeta analyzer. These platinum nanoparticles have a spherical shape and FCC structure with an average particle size of 3.4 nm and an average hydrodynamic diameter of 248 nm. The characteristic FTIR peaks of chitosan in the sample confirmed the capping of chitosan on the surface of the Pt NPs. The surface charge estimation using a zeta potential analyzer showed −23.8 mV, elucidating the stability and dispersity of the as-synthesized Pt NPs. The in vitro cytotoxicity study using MTT assay revealed a non-toxic behaviour on normal L929 cell lines and a severe anti-proliferative activity on a human breast cancer (MCF-7) cell line with an IC50 value of 35.60 μg/ml after 24 h of incubation. This result indicates a better anticancer therapeutic application against human breast cancer cells for the as-synthesized chitosan-stabilized platinum nanoparticles.