Elution band profiles were determined experimentally for the racemic mixture of d- and l-mandelic acid on a bovine serum albumin chemically bonded phase. The results show significantly different profiles for a given amount of either isomer, whether pure or in the racemic mixture. The production rate at 99% purity is more than one order of magnitude higher under overlapping band than under touching band conditions. In the former instance, the recovery yield and production rate are always higher for the less retained enantiomer.