L-lysine HCl Research Articles

Morphometric analysis of the effects of high molecular weight sodium hyaluronate and amino acids mixture on face rejuvenation.

Skin aging can be observed at various levels in the epidermis, dermis, and dermo-epidermal junction. Reducing the cosmetic effects of skin aging in the facial region is a widespread demand due to common aesthetic concerns. Consequently, many injectable products on the market promise antiaging effects and cosmetic improvements. We aimed to evaluate the cosmetic efficacy of a high molecular weight sodium hyaluronate and amino acids mixture for the facial region using morphometric analysis. This study evaluates the morphometric effectiveness of an injectable mixture (high molecular weight sodium hyaluronate, glycine, L-Proline, L-leucine, L-lysine HCL, L-valine, and L-alanine collagen active ingredient) on the mid-face and jawline in women aged 30-55. We used morphological measurements and digital image data to assess changes and determine effectiveness. Various computational methods were applied simultaneously with statistical tests for validation. The hydration assessment showed a significant increase on both sides of the face. A noticeable decrease was observed in gonion angle, bitragion breadth, bigonion breadth, and marionette wrinkle scale. These results suggest combining mechanical and chemical stimulation from the injection and its components (hyaluronic and amino acids) effectively enhances skin quality. The study indicates that the mechanical stimulation of the injection improves skin quality. Combining hyaluronic and amino acids (collagen, elastin, and pro-synthetic) is a safe and effective alternative for antiaging treatments.

Requirement for digestible lysine in Nile tilapias (Oreochromis niloticus) with live weight between 500 and 600 g

The present study was carried out with the objective of determining the requirements for digestible lysine in Nile tilapia during the 500 to 600 g live weight stage. Isocaloric and isonitrogenous diets were tested with increasing amounts of digestible lysine. L-lysine HCl (78%) were used to replace glutamic acid at increasing levels, resulting in treatments of 9.3, 12.3, 15.3, 18.3 and 21.3 g kg-1 of digestible lysine. Three hundred Nile tilapias with an average weight of 519 ± 27.23 g were used and distributed among 25 tanks. The physical and chemical parameters of the water were pH, dissolved oxygen, salinity, conductivity and temperature. The mortality rate was registered daily. Two slaughters were performed at 28 and 50 days after the beginning of the experiment. It was estimated that an amount of 13.1 g kg-1 of digestible lysine was ideal for obtaining higher WG. Fish slaughtered after 50 days, the digestible lysine requirements were determined to be 14.5 g kg-1 for the lowest carcass humidity and 14.6 g kg-1 for the greatest carcass ethereal extract. Nile tilapias with live weight between 500 and 600 grams require 13.1 g kg-1 of digestible lysine in the diet for greater WG (90.53 g) and better FCR (2.55).

100 Methane Production in Roughage-Lacking Diets Containing Ionized Amino Acids as Antibiotic Alternative

Abstract The use of antibiotics in animal feed, commonly used as a strategy to achieve greater daily weight gains and recently as way to reduce methane emission, might result in the selection of resistant microorganisms and certain bacterial strains isolated from cattle are resistant to multiple antibiotics and could be human health hazards. To evaluate methane production in ruminant diet without roughage containing ionized amino acids as antibiotic alternative, twenty-four Nellore young bulls were allotted into two groups. The first group was fed with high-grain diet without roughage and without antibiotics diet, containing 82 mg/kg of ionized amino acids mixture (L-lysine and L-taurine HCl 99%, pH 9.74) manufactured by MJ Animal Nutrition. The second group was fed with high-grain diet containing Brachiaria brizantha hay (15% dry matter) and sodium monensin (120 mgּּ animal -1 ּּ day-1). It was not used a negative control group to avoid metabolic disturbances. The feedlot time was 100 days. The weight measurements were performed at the beginning of the trial period and monthly, always with water restriction and fasting for 18 hours, even before slaughter. A completely randomized design was used. Methane emission was measured using the sulfur hexafluoride technique and daily dry matter intake were measured in GrowSafe system. There was no detection of lameness or laminitis in any groups and there were no changes in feed conversion ratio (FCR). The total methane emission (-77.11%) and the methane emission per dry matter intake (DMI; -57.12 %), per average daily weight gain (ADG; -71.11%) and per kilogram of carcass (-70.07%) were different between treatments (P < 0.0001), resulting in decreased emission intensities for group fed without roughage with diet containing ionized amino acids. We conclude that fed without roughage with diet containing ionized amino acids was an effective strategy as an antibiotic alternative, mitigate GHG emissions and facilitate feedlot diets management. In field conditions, the roughage-lacking diets represent advantage, especially regarding its administration to a large amount of the animals just once a week or less, no need area, infra-structure and expertise to produce, preserve and feeding roughage.

Ionozymes for Efficient Synthesis of Cadaverine: Offering a Sustainable Way for Bio-nylon 5X Production

Bio-based cadaverine, a crucial monomer for the production of bio-nylon 5X, can be synthesized during the bioconversion of l-lysine HCl relying on lysine decarboxylases. The rationally designed lysine decarboxylase ΔLdcEt3 has exhibited outstanding alkaline stability in pH 8.0 (half-life: 362 h); however, its catalytic activity still needs to be improved to meet the requirements of industrial cadaverine production. A novel ionozyme strategy can create a preferable reaction microenvironment, affect the intermediate formation, and/or improve the enzymatic stability and activity. Therefore, ionozymes ΔLdcEt3-[Emim]Cl and ΔLdcEt3-[Ch][Ser] have been successfully developed for efficient cadaverine production in this study. The results showed that the catalytic activities of ΔLdcEt3-[Emim]Cl and ΔLdcEt3-[Ch][Ser] improved 124.2 and 116.2%, respectively. Meanwhile, the catalytic efficiencies (kcat/Km) of ΔLdcEt3-[Emim]Cl and ΔLdcEt3-[Ch][Ser] were also increased by 1.5- and 1.2-fold, respectively. Particle size, circular dichroism, and Raman spectrum analyses showed that [Emim]Cl and [Ch][Ser] could affect short-range attractions related to the aggregation state and change the secondary structure. Protein surface analysis demonstrated that the addition of ionic liquids changed the hydrophobicity of ΔLdcEt3. In addition, isothermal titration calorimetry and molecular docking revealed that [Emim]Cl and [Ch][Ser] could promote the protein–ligand complexation during the enthalpy-driven l-lysine and ΔLdcEt3 binding process, which was confirmed by molecular dynamics. Therefore, ionozymes ΔLdcEt3-[Emim]Cl/[Ch][Ser] provide a novel possibility for high-level cadaverine production.

Effect of L-Methionine and L-Lysine HCL supplementation in minimum standard feed requirements on the performance of KUB (Kampung Unggul Balitbangtan) chickens

This study will determine the effect of supplementing L-methionine, and L-lysine HCl to a minimum diet based on KUB (Kampung Unggul Balitbangtan) chickens standards body weight, weight gain, and Feed Conversion Ratio of KUB chicken at the grower phase. A total of 240 Eight weeks-old KUB chickens were used in this study. The KUB chickens were divided into 3 treatments with 4 replications. The dietary treatments were: R0 (ration based on minimum diet standard), R1 (ration based on minimum diet standard + 0.25% L-Methionine+ 0.25% L-Lysine HCl), R2 (ration based on minimum diet standard + 0.5% L- Methionine + 0.5% L-Lysine HCl). The data collected were body weight, weight gain, and feed conversion ratio of KUB chickens aged 8 weeks. The result of this study for R0, R1 and R2 showed that body weight of chickens were 516.25+34.07a; 519.75+31.25a; 502+47.45bg/bird, weights gain were451.58+39.54a; 443.08+35.74ab; 424.83+57.73bg/birds and feed conversion ratio were3.76+0.31a; 3.83+0.29a; 4.05+0.63brespectively. The results showed that supplementation of L-Methionine and L-Lysine HCl using minimum diet standard gave no significant effect (P<0.05) on body weight, weight gain, and Feed Conversion. The result concluded that supplementing 0.25% to 0.5% L-methionine and l-lysine HCl did not increase body weight and weight gain and did not affect the Feed Conversion of KUB chickens. Furthermore, the minimum diet standard for KUB chicken already has a basic diet requirement. We suggested not adding the combination of L-Methionine (0.25% to 0.5%) and L-Lysine HCl (0.25% to 0.5%) in the minimum diet standard KUB chicken.

Safety and efficacy of a feed additive consisting of concentrated liquid l-lysine, l-lysine monohydrochloride and concentrated liquid l-lysine monohydrochloride produced by Escherichia coliNITE BP-02917 for all animal species (Metex NoovistaGo).

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of concentrated liquid l‐lysine, l‐lysine monohydrochloride and concentrated liquid l‐lysine monohydrochloride produced by Escherichia coli NITE BP‐02917 as nutritional and as sensory (flavouring compound) feed additives for all animal species. The production strain did not carry ■■■■■ antimicrobial resistance genes and no viable cells of the production strain were detected in the final products. ■■■■■ However, since no sequences of concern remained in the production strain, the potential presence of that DNA did not raise safety concerns. The use of the three forms of l‐lysine produced by E. coli NITE BP‐02917 in supplementing feed to compensate for l‐lysine deficiency in feedingstuffs was safe for the target species. This conclusion would also cover the use as a sensory additive. The FEEDAP Panel identified risks of nutritional imbalances and hygienic concerns for amino acids when administered simultaneously in feed and in water for drinking. The use of the three forms of l‐lysine produced by E. coli NITE BP‐02917 in animal nutrition was considered safe for the consumers and for the environment. Concentrated liquid l‐lysine, l‐lysine HCl and concentrated liquid l‐lysine HCl were not considered to have the potential to cause respiratory toxicity, or skin sensitisation. l‐Lysine HCl and concentrated liquid l‐lysine HCl were not considered skin and eye irritants. Concentrated liquid l‐lysine, due to its high pH, might be corrosive for skin and eyes. The three forms were considered an efficacious source of the essential amino acid l‐lysine for non‐ruminant animal species. For the supplemental l‐lysine to be as efficacious in ruminants as in non‐ruminant species, it would require protection against degradation in the rumen. The three forms of the additive were also considered efficacious as feed flavouring compounds under the proposed conditions of use.

Safety and efficacy of a feed additive consisting of l-lysine monohydrochloride and l-lysine sulfate produced by fermentation with Corynebacterium glutamicumCGMCC 17927 for all animal species (Barentz Animal Nutrition B.V.).

Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on l‐lysine monohydrochloride and l‐lysine sulfate produced by Corynebacterium glutamicum CGMCC 17927, when used as a nutritional additive in feed and water for drinking for all animal species. The active substance is l‐lysine, and it was produced in two different forms: monohydrochloride (HCl) or sulfate salts. The production strain was genetically modified. Neither viable cells nor recombinant DNA of the production strain were detected in the final products. Therefore, the Panel concluded that the additives did not pose any safety concern regarding the production strain. The use of l‐lysine HCl and l‐lysine sulfate produced by the strain C. glutamicum CGMCC 17927 in supplementing feed to compensate for l‐lysine deficiency in feedingstuffs was safe for the target species. The FEEDAP Panel identified risks of nutritional imbalances and hygienic concerns for amino acids when administered simultaneously in feed and in water for drinking. The use of both forms of l‐lysine produced by fermentation using C. glutamicum CGMCC 17927 in animal nutrition was considered safe for the consumers and for the environment. Exposure of users through inhalation to l‐lysine HCl and l‐lysine sulfate produced with C. glutamicum CGMCC 17927 was considered very likely. In absence of data, the FEEDAP Panel could not conclude on the potential of both forms of the additive to be irritant for skin and eyes or to be dermal sensitisers. l‐Lysine HCl and l‐lysine sulfate were considered as efficacious sources of the essential amino acid l‐lysine for non‐ruminant animal species. For the supplemental l‐lysine to be as efficacious in ruminants as in non‐ruminant species, it would require protection against degradation in the rumen.

Effect of L-arginine and L-Lysine HCl ratio on growth performance and ileum morphology of native chickens aged 2-14 weeks.

Background and Aim:Micronutrients such as essential amino acids in chicken feed must be balanced to promote optimal development. The balance of the amino acids arginine and lysine in chicken feed is particularly important. This study aimed to examine the effect of the ratio of L-arginine to L-Lysine HCl on growth performance and ileum morphology of native chickens aged 2-14 weeks-old.Materials and Methods:One hundred and eighty 2-week-old native chickens which initial weight 78.10±4.97 g were classified into six treatments and five repetitions using a completely randomized design.Treatments were based on the ratio of arginine to lysine in the feed:T1 (0.50% L-arginine: 0.85% L-lysine HCl); T2 (0.75% L-arginine: 0.85% L-lysine HCl); T3 (1.00% L-arginine: 0.85% L-lysine HCl); T4 (0.50% L-arginine: 1.00% L-lysine HCl); T5 (0.75% L-arginine: 1.00% L-lysine HCl); and T6 (1.00% L-arginine: 1.00% L-lysine HCl).Results:Groups T3 and T6 had the highest feed consumption (42.06±0.29 and 42.78±0.72 g/bird/day, respectively), while Group T6 had the highest body weight and body weight gain rate (1505.60±103.20 kg/bird and 16.99±1.24 g/bird/day, respectively). Groups T3 and T6 also had the highest carcass weight (916.16±46.99 and 947.18±62.32 g/bird, respectively). The best feed conversion was seen for Groups T3, T5, and T6 (2.55±0.14, 2.50±0.20, and 2.53±0.19, respectively). For ileum morphometry, the highest villus height occurred in Groups T2, T3, T5, and T6 (962.80±23.31, 982.80±10.03, 972.80±18.99, and 989.80±10.69 μm, respectively); and Group T6 had the highest crypt depth and villus width (340.80±11.52 and 302.00±4.00 μm, respectively). Statistical analysis indicated significant differences among the treatment groups for all variables examined (p<0.05).Conclusion:The highest ratio of arginine-lysine was associated with the largest increase in native chicken feed consumption, body weight gain, feed conversion, and carcass weight, as well as villus height and width, and crypt depth in the ileum. Overall, an arginine-lysine ratio of 0.8-1.20 promoted optimal growth of native chickens aged 2-14 weeks. In the future, it is important to increase the arginine-lysine ratio with low feed protein levels in native chickens.

PSIV-9 Effect of Increasing the Level of a Modified Corn Protein on Nursery Pig Growth Performance, Feed Efficiency, and Fecal Dry Matter

Abstract A total of 360 barrows (DNA Line 200×400; initially 6.1±0.05 kg) were used in a 38-d study to evaluate the effects of increasing levels of a modified corn protein product on nursery pig growth performance and fecal dry matter. Upon arrival to the nursery research facility, pigs were randomly assigned to pens (5 pigs/pen) and pens were randomly allotted to 6 dietary treatments (12 pens/treatment). Experimental diets were fed in 2 phases. Phase 1 (d 0-10) diets were formulated to contain 3, 6, 9, 12, or 15% of a modified corn protein or 6% enzymatically treated soybean meal (ESBM). Additions of the modified protein product and ESBM were reduced by 50% for phase 2 (d 10 to 25) diets and were: 1.5, 3.0, 4.5, 6.0, 7.5, and 3.0%, respectively. Nutrient loading values were provided by the supplier and diets were formulated to maximize L-Lysine HCl while keeping SID Lys:CP and SID AA:Lys similar across treatments. A common phase 3 diet was fed from d 25-38. During the experimental period (d 0-25), ADG (quadratic, P = 0.030) and ADFI (quadratic, P = 0.036) increased, and G:F worsened (linear, P = 0.006) as the amount of modified corn protein increased. For the overall experiment (d 0-38), ADG (quadratic, P = 0.028) and ADFI (quadratic, P = 0.032) increased then decreased and G:F tended to decrease (linear, P = 0.066) with increasing modified corn protein. There was a marginally significant treatment×day interaction (quadratic, P = 0.064) where fecal DM tended to increase (P = 0.051) on d 25 as the level of the modified corn protein was increased, but no evidence for change on d 10 (P &amp;gt; 0.10). These data suggest that the modified corn protein tested in this trial may be an alternative protein source to consider for nursery pig diets, when fed up to 12% in phase 1 and 6% in phase 2.

Safety and efficacy of a feed additive consisting of l-lysine monohydrochloride and l-lysine sulfate produced by Corynebacterium glutamicum CGMCC 14498 for all animal species (Kempex Holland BV).

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of l‐lysine monohydrochloride (l‐lysine HCl) and l‐lysine sulfate produced by Corynebacterium glutamicum (C. glutamicum) CGMCC 14498 as a nutritional feed additive for all animal species. The active substance is l‐lysine and it is produced in two different forms (monohydrochloride or sulfate). The production strain C. glutamicum CGMCC 14498 and its recombinant DNA were not detected in the final products. The products l‐lysine HCl and l‐lysine sulfate do not pose any safety concern associated with the production strain. l‐Lysine HCl and l‐lysine sulfate produced by C. glutamicum CGMCC 14498 are considered safe for the target species. When using l‐lysine sulfate, the background sulfur/sulfate content in the compound feed should be taken into account. l‐Lysine HCl and l‐lysine sulfate produced by C. glutamicum CGMCC 14498 are safe for the consumer and the environment. In the absence of data, the FEEDAP Panel cannot conclude on the potential of l‐lysine HCl produced by the strain C. glutamicum CGMCC 14498 to be toxic by inhalation, and on the potential of l‐lysine HCl and l‐lysine sulfate produced by the above‐mentioned strain to be irritant to skin or eyes, or on their potential to be dermal sensitisers. l‐Lysine HCl and l‐lysine sulfate produced by C. glutamicum CGMCC 14498 are considered efficacious sources of the essential amino acid l‐lysine for non‐ruminant animal species. For the supplemental l‐lysine to be as efficacious in ruminants as in non‐ruminant species, this would require protection against degradation in the rumen.

Coproduction of 5-Aminovalerate and δ-Valerolactam for the Synthesis of Nylon 5 From L-Lysine in Escherichia coli.

The compounds 5-aminovalerate and δ-valerolactam are important building blocks that can be used to synthesize bioplastics. The production of 5-aminovalerate and δ-valerolactam in microorganisms provides an ideal source that reduces the cost. To achieve efficient biobased coproduction of 5-aminovalerate and δ-valerolactam in Escherichia coli, a single biotransformation step from L-lysine was constructed. First, an equilibrium mixture was formed by L-lysine α-oxidase RaiP from Scomber japonicus. In addition, by adjusting the pH and H2O2 concentration, the titers of 5-aminovalerate and δ-valerolactam reached 10.24 and 1.82 g/L from 40 g/L L-lysine HCl at pH 5.0 and 10 mM H2O2, respectively. With the optimized pH value, the δ-valerolactam titer was improved to 6.88 g/L at pH 9.0 with a molar yield of 0.35 mol/mol lysine. The ratio of 5AVA and δ-valerolactam was obviously affected by pH value. The ratio of 5AVA and δ-valerolactam could be obtained in the range of 5.63:1–0.58:1 at pH 5.0–9.0 from the equilibrium mixture. As a result, the simultaneous synthesis of 5-aminovalerate and δ-valerolactam from L-lysine in Escherichia coli is highly promising. To our knowledge, this result constitutes the highest δ-valerolactam titer reported by biological methods. In summary, a commercially implied bioprocess developed for the coproduction of 5-aminovalerate and δ-valerolactam using engineered Escherichia coli.

143 Impact of Replacing Soybean Meal with Corn DDGS and Crystalline Amino Acids on Performance and Carcass Characteristics of Growing Pigs

Abstract This study evaluated the effect of replacing soybean meal (SBM) with DDGS and crystalline amino acids on growth and carcass lean. Pigs (n = 512; 38.51±0.13 kg BW) were blocked by BW and sex and placed in 64 pens (4 gilts and barrows per pen). Treatments were arranged as a 2×4 factorial with DDGS included at 0 or 25% and L-lysine-HCl (LYS) added at 0, 0.2, 0.4, and 0.6%. Dietary SBM inclusion declined as LYS increased from 32.06 to 13.14% (Phase 1) and 28.25 to 9.40% (Phase 2) for control diets. It declined from 27.85 to 8.89% (Phase 1) and 24.05 to 5.10% (Phase 2) for DDGS diets. Diets contained 1.00 (Phase 1, 21 days) and 0.90% (Phase 2, 18 days) SID lysine and were balanced for ideal protein and net energy. During Phase 1, DDGS decreased ADG (P = 0.06; 786 vs. 821 g/d). Increasing LYS increased (quadratic, P ≤ 0.05) ADG and ADFI with the greatest response at 0.4% LYS. G:F decreased (linear, P = 0.035) with increasing LYS. During Phase 2, increasing LYS in control, but not DDGS diets, decreased (linear, P &amp;lt; 0.005) ADG and ADFI. G:F declined (P = 0.054) with DDGS inclusion (370 vs. 383 g/kg). Overall, ADG decreased (linear, P = 0.005) as LYS increased in control (959, 929, 908, 860 g/d), but not DDGS diets (863, 908, 931, 832 g/d). ADFI decreased (linear, P = 0.014) with increasing LYS in control (2270, 2198, 2186, 2130 g/d), but increased (quadratic, P = 0.039) in DDGS diets (2112, 2207, 2324, 2103 g/d). DDGS reduced (P≤0.03) ADG (883 vs. 914 g/d), G:F (405 vs. 417 g/kg) and LEA (34.5 vs. 35.3 cm2), while increasing LYS decreased (linear, P &amp;lt; 0.005) G:F (417, 419, 409, 401 g/kg) and LEA (35.34, 35.17, 35.46, 33.64 cm2). Displacement of SBM with DDGS reduced growth and LYS addition negatively affected growth and G:F for diets with SBM, but not DDGS.

Safety and efficacy of the feed additives concentrated liquid l-lysine (base) and l-lysine monohydrochloride produced by Corynebacterium glutamicum KCCM 80183 for all animal species (CJ Europe GmbH).

Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on concentrated liquid l‐lysine (base) and l‐lysine monohydrochloride (HCl) produced using Corynebacterium glutamicum KCCM 80183 when used as nutritional additives in feed and water for drinking for all animal species. The active substance is l‐lysine. Concentrated liquid l‐lysine (base) and l‐Lysine HCl produced by the strain C. glutamicum KCCM 80183 do not pose any safety concern as regards the genetic modification of the production strain. The use of the additives under assessment in supplementing feed to compensate for l‐lysine deficiency in feedingstuffs is safe for the target species. The FEEDAP Panel has concerns about the use of amino acids in water for drinking for hygienic reasons, and due to the risk of imbalances when administered simultaneously via feed. The use of both forms of l‐lysine produced by fermentation using C. glutamicum KCCM 80183 in animal nutrition is considered safe for the consumers and for the environment. Concentrated liquid l‐lysine (base) produced by C. glutamicum KCCM 80183 is considered hazardous by inhalation, not irritant to skin and eyes and it is not a skin sensitiser. l‐Lysine HCl produced by C. glutamicum KCCM 80183 is considered hazardous by inhalation, it is not irritant to skin but mildly irritant to eyes and it is not a skin sensitiser. The additives under assessment are considered as efficacious sources of the essential amino acid l‐lysine for non‐ruminant animal species. For the supplemental l‐lysine to be as efficacious in ruminants as in non‐ruminant species, it would require protection against degradation in the rumen.

Safety and efficacy of l-lysine monohydrochloride and concentrated liquid l-lysine (base) produced by fermentation with Corynebacterium glutamicum KCCM 80216 as feed additive for all animal species.

Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the safety and efficacy of concentrated liquid l‐lysine (base, minimum 50%) and l‐lysine monohydrochloride (HCl, minimum 99%) produced by fermentation with a genetically modified strain of Corynebacterium glutamicum (KCCM 80216) as nutritional additives for all animal species. Neither the production strain nor its recombinant DNA was detected in the final products. The additives do not pose any safety concern associated with the genetic modification of the production strain. Concentrated liquid l‐lysine (base) and l‐lysine HCl produced by C. glutamicum KCCM 80216 do not represent a risk for the target species, the consumer and the environment. From the results of studies on the safety for the user of concentrated liquid l‐lysine (base) and l‐lysine HCl produced by a different production strain, it was possible to conclude on the safety for the user of the products under assessment. The concentrated liquid l‐lysine (base) and the l‐lysine HCl are not irritant to skin or eyes or skin sensitiser. l‐lysine HCl is not hazardous by inhalation. l‐lysine HCl and concentrated liquid l‐lysine (base) are considered as efficacious sources of the essential amino acid l‐lysine for non‐ruminant animal species. For the supplemental l‐lysine to be as efficacious in ruminants as in non‐ruminant species, it would require protection against degradation in the rumen.

Safety and efficacy of l-lysine monohydrochloride and concentrated liquid l-lysine (base) produced by fermentation with Corynebacterium glutamicumKCTC 12307BP as feed additives for all animal species.

Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the safety for the target animals, consumer, user and the environment and on the efficacy of a concentrated liquid l‐lysine (base, minimum 50%) and a l‐lysine monohydrochloride (HCl, minimum 99%) produced by fermentation with a genetically modified strain of Corynebacterium glutamicum (KCTC 12307BP). Both forms of l‐lysine are intended to be used in feed for all animal species and categories. Neither the production strain nor its recombinant DNA were detected in the final products. The additives do not pose any safety concern associated with the genetic modification of the production strain. Concentrated liquid l‐lysine (base) and l‐lysine HCl produced by the strain C. glutamicum KCTC 12307BP do not represent a risk for the target species, for the consumer, for the user and for the environment. l‐Lysine HCl and concentrated liquid l‐lysine (base) are considered to be efficacious sources of the essential amino acid l‐lysine for non‐ruminant animal species. For the supplemental l‐lysine to be as efficacious in ruminants as in non‐ruminant species, it would require protection against degradation in the rumen.

PSII-19 Effect of inclusion level of synthetic lysine, threonine, methionine, and tryptophan in corn-soybean meal and DDGS based diets for grow-finish pigs on growth performance and carcass characteristics

Abstract The objective of this study was to determine the effect of increasing dietary synthetic amino acids L-Lysine HCl, L-Threonine, Alimet®, and L-Tryptophan inclusion level at the expense of soybean meal on growth performance during the grow-finish period (46.2 ± 0.55 to 127.4 ± 0.83 kg BW) and carcass characteristics of pigs reared under commercial conditions. The study used a RCBD with 2 dietary treatments: 1). Control: 0.309, 0.029, 0.028, and 0.000 g/kg of synthetic lysine, methionine, threonine, and tryptophan, respectively; 2). High Inclusion: 0.422, 0.069, 0.047, and 0.014 g/kg, respectively. Diets were corn-soybean meal based with DDGS (20.6% for both diets) formulated to be isocaloric and to the same SID amino acid levels but varied in soybean meal inclusion (14.1 and 10.2%, for the Control and High Inclusion, respectively) and crude protein content (17.7 and 16.4%, respectively). A total of 3,520 barrows and gilts were used, housed in single-sex pens of 32 (22 replicates) at a floor space of 0.67 m2/pig. Pigs had ad libitum access to feed and water throughout the study. Pen was the experimental unit and data were analyzed using PROC MIXED of SAS; the model accounted for fixed effect of dietary treatment and the random effect of block. There was no effect of dietary treatment on final live weight, overall ADFI, hot carcass weight, backfat depth, or longissimus muscle depth. However, the High Inclusion treatment had lower overall ADG (P ≤ 0.01) and overall G:F (P ≤ 0.05), but greater (P ≤ 0.01) carcass yield than the Control treatment. These results suggest that increasing the inclusion of synthetic amino acids in corn-soybean and DDGS based diets for growing-finishing pigs to the levels evaluated negatively impacted growth rate and feed efficiency but increased carcass yield.

Safety and efficacy of concentrated liquid l-lysine (base) and l-lysine monohydrochloride produced by fermentation with Corynebacterium casei KCCM 80190 as feed additives for all animal species.

Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on concentrated liquid l‐lysine (base) and l‐lysine monohydrochloride (HCl) produced using Corynebacterium casei KCCM 80190 when used as nutritional additives in feed and water for drinking for all animal species. The active substance is l‐lysine. The production strain is genetically modified. It does not carry acquired antimicrobial resistance genes and no viable cells of the production strain nor its DNA were detected in the final products. Therefore, the additives do not pose any safety concern regarding the genetic modifications. Concentrated liquid l‐lysine (base) and l‐lysine HCl produced by C. casei KCCM 80190 do not represent a risk for the target species, the consumer and the environment. From the results of studies on the safety for the user of concentrated liquid l‐lysine (base) and l‐lysine HCl produced by a different production strain, it was possible to conclude on the safety for the user of the products under assessment. Concentrated liquid l‐lysine (base) produced by C. casei KCCM 80190 is considered hazardous by inhalation, not irritant to skin and eyes and it is not a skin sensitiser. l‐Lysine HCl produced by C. casei KCCM 80190 is considered hazardous by inhalation, it is not irritant to skin but mildly irritant to eyes and it is not a skin sensitiser. Concentrated liquid l‐lysine (base) and l‐lysine HCl are considered efficacious sources of the essential amino acid l‐lysine for non‐ruminant animal species. For the supplemental l‐lysine to be as efficacious in ruminants as in non‐ruminant species, it would require protection against degradation in the rumen.

THE EFFECT OF L-LYSINE HCL SUPPLEMENTATION ON THE BODY WEIGHT GAIN, FEED CONSUMPTION AND FEED EFFICIENCY OF BROILERS

The purpose of this study was to determine the effect of l-lysine supplementation in feed on the body weight gain, feed consumption, and feed efficiency of broilers. The experiment was conducted at the Laboratory of Animal Husbandry Study Program, Agriculture Faculty, University of Timor, Kefamenanu from December 2019 to January 2020. This study used 80 day-old chicks (DOC) broilers. The method used is a Completely Randomized Design with four treatments and four replications. The treatments give were: T0 (control feed), T1 (control feed + 0.35% l-lysine), T2 (control feed + 0.70% l-lysine), T3 (control feed + 1.00% l -lysine). Data were processed using Variance Analysis, and Duncan test. The results of the statistical analysis showed that the supplementation of l-lysine in the feed had a significant effect on weight gain, feed consumption, and feed efficiency (P&lt;0.05). It was concluded that supplementation of 0.70% l-lysine HCl in feed gave optimum results to weight gain, feed consumption, and feed efficiency of broilers.

Safety and efficacy of l-lysine monohydrochloride produced by fermentation with Corynebacterium glutamicum DSM 32932 for all animal species.

Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on l‐lysine monohydrochloride produced by fermentation with the genetically modified strain of Corynebacterium glutamicum DSM 32932. Neither the production strain nor its recombinant DNA were detected in the final product. The additive does not pose any safety concern associated with the genetic modification of the production strain. l‐Lysine HCl produced by C. glutamicum DSM 32932 is considered safe for the target species, for the consumer and for the environment. l‐Lysine HCl produced by C. glutamicum DSM 32932 is not toxic by inhalation; it is not irritant to skin and not a skin sensitiser. The additive is not corrosive to eyes but it should be considered as an eye irritant. In general, l‐lysine HCl is considered as an efficacious source of the essential amino acid l‐lysine for non‐ruminant animal species. For the supplemental l‐lysine to be as efficacious in ruminants as in non‐ruminant species, it would require protection against degradation in the rumen.

Safety and efficacy of l-lysine monohydrochloride and concentrated liquid l-lysine (base) produced by fermentation using Corynebacterium glutamicum strains NRRL-B-67439 or NRRL B-67535 for all animal species.

The European Commission asked EFSA for an opinion on the safety for the target animals, consumer, user and the environment and on the efficacy of a l‐lysine monohydrochloride (HCl, minimum 98.5%) and of a concentrated liquid l‐lysine (base, minimum 50%) produced by genetically modified strains of Corynebacterium glutamicum (NRRL‐B‐67439 or NRRL B‐67535). They are intended to be used in feed or water for drinking for all animal species and categories. Neither viable cells of the production strains C. glutamicum strains NRRLB‐67439 or NRRL B‐67535; nor their recombinant DNA were detected in the final products. Therefore, those products do not pose any safety concern associated with the genetic modification of the production strains. l‐Lysine HCl and concentrated liquid l‐lysine (base) produced by C. glutamicum strains NRRLB‐67439 or NRRL B‐67535 are considered safe for the target species, for the consumer and for the environment. l‐Lysine HCl produced by C. glutamicum strains NRRL B‐67439 or NRRL B‐67535 is considered not irritant to skin or eyes and not a skin sensitiser. In the absence of data, the FEEDAP Panel cannot conclude on the potential toxicity by inhalation of l‐lysine HCl produced by C. glutamicum strains NRRL B‐67439 or NRRL B‐67535. Concentrated liquid l‐lysine (base) produced by C. glutamicum strains NRRL B‐67439 or NRRL B‐67535, due to its high pH (10.7 and 10.9, respectively) is anticipated to be corrosive to skin and eyes and poses a risk by inhalation. l‐Lysine HCl and concentrated liquid l‐lysine (base) produced by C. glutamicum strains NRRLB‐67439 or NRRL B‐67535 are considered as efficacious sources of the essential amino acid l‐lysine for non‐ruminant animal species. For the supplemental l‐lysine to be as efficacious in ruminants as in non‐ruminant species, it would require protection against degradation in the rumen.