The present study encompasses isolation of marine bacteria from marine soil samples collected from Chirala, Coastal area of Andhra Pradesh. Work includes isolation, screening, morphological and molecular characterization of selected L- Asparaginase producers. Purified enzyme was used for enzyme kinetic studies. Marine soil sample was collected in sterile bottle and brought to the lab bench and a total of 12 morphologically distinct bacterial colonies were isolated from collected marine soil samples and coded as Chirala Marine Bacteria. All 12 Chirala Marine Bacteria isolates were qualitatively screened for L-Asparaginase production. Out of 12 Chirala marine bacteria isolates, 9 isolates show positive response to L-Asparaginase activity. Effect of inducers i.e. carbon, nitrogen, aminoacids, phosphates and metal ions was studied.