ObjectiveTo collect health information of Beijing middle-aged registered nurses during menopause. MethodsWe distributed self-administered questionnaires to 2100 registered nurses aged 40–55 from 20 hospitals in Beijing. The objects of interest were selected by cluster sampling. ResultsA total of 1686 questionnaires met the criteria and were used for statistical analysis. The average natural menopause age was 48.68±3.61 years old. We determined that 37.83% of the objects had modified Kupperman Menopausal Index (KMI) scores ≥15. The top three menopause symptoms were fatigue (82.72%), irritability (70.24%), and arthralgia/myalgia (69.55%); hot flashes ranked eleventh (30.83%). ConclusionsA total of 37.83% Beijing middle-aged registered nurses had menopause syndrome, and the top three symptoms were fatigue, irritability, and arthralgia/myalgia.