Colletotrichum gloeosporioides, the causal agent of anthracnose, is the main postharvest pathogen in papaya. This study, therefore, evaluates the effect of five fungicides to control this disease in papaya cv. Maradol. The experiment comprised two stages: in vivo and in vitro, in which the fungicides benomyl, imazalil, prochloraz, azoxystrobin, kresoxim methyl were evaluated. The results of the first stage indicated that prochloraz was the fungicide that offered greater perspectives in the control of anthracnose with an average effectiveness of 96.5%, while benomyl was the least efficient, (15.71%). In the second stage, the fungicide prochloraz had the smallest CE95 with 7.91 µg·ml-1 during conidial germination and the smallest CE50 and CE95 for the mycelia growth with 0.0002 µg·ml-1 and 1.46-µg·ml-1, respectively. However, azoxystrobin obtained greater CE50 and CE95 for the mycelia growth, with 258.25 µg·ml-1 , and 365.25 µg·ml-1, respectively. It is concluded that prochloraz was the fungicide that best controlled both anthracnose and C. gloeosporioides germination and mycelia growth.