Krasnodar Territory Research Articles

Anthropogenic Factor as a Relief-forming Factor of the Coastal Zone of the Black Sea Coast of the Krasnodar Territory (On the Example of the Tuapse-Adler Coastline)

The ever-increasing technogenic and anthropogenic load on the seacoasts, which significantly affects natural coastal processes, is increasingly beginning to act as a determining relief-forming factor. Hydraulic structures erected in the coastal zone have a dual effect on the natural coastal processes. On the one hand, they themselves are an artificially created relief, and on the other hand, the structures being erected have a significant effect on natural coastal processes. The example of the coastal section between Tuapse and Adler, located within the Black Sea coast of Krasnodar Territory, which experiences enormous technogenic and anthropogenic load on the coast, shows the need to identify anthropogenic coastal dynamic systems as an independent relief-forming factor. At the same time, an assessment should be made of both the processes occurring within the system and its impact on the natural processes occurring in the adjacent coastal areas.

Complete mitochondrial genome of <I>Clupeonella abrau</I> (Maliatsky, 1930) (Clupeiformes), an endemic species of freshwater fish from lake Abrau (Russia)

The Abrau sprat (Tyulka or Sardelka) Clupeonella abrau (Maliatsky, 1930) is an endemic fish of Lake Abrau (Krasnodar Territory). The full mitogenome of C. abrau (16650 bp in length, with a conservative gene arrangement for Clupeidae) demonstrates 98.8% similarity with the mitogenome of a related species, the Black Sea-Caspian tulka (C. cultriventris) from the Black Sea. The sequence of the COX1 gene was also studied in a museum specimen collected in Lake Abrau in 1938. Variability in modern Abrau sprat COX1 locus in about 0.15%, the difference between C. abrau and C. cultriventris is 1.2%, and the difference between the Museum and modern samples of tulka from Lake Abrau is 0.92%. It has been confirmed that the Abrau sprat is present in the fish community and capable of reproducing in the lake. Various alternative scenarios for the settlement of Lake Abrau have been proposed.

Standardization of studies on physical development of children in the Russian Federation

BACKGROUND:The importance of studying the growth and development processes of the child population is due to the significance of the pre-clinical approach aimed at primary disease prevention. AIM:To provide the constituent entities of the Russian Federation with standards for the physical development of children and adolescents aged 7–17years. MATERIAL AND METHODS:An analytical study was conducted, big data from the database “Physical Development of Children, Adolescents, and Youth of the Russian Federation in 2000–2021” were analyzed, containing 395,956observations of physical development (length and body weight indicators) of children, adolescents, and young people living in 49constituent entities of the Russian Federation. Priority was given to research in the constituent entities for which regional standards had never been developed or for which they were developed 15–20years or more ago. As a result, at least 2constituent entities were analyzed in each federal district. Big data processing using machine learning was carried out on the basis of the PostgreSQL database. A web application was created that implemented the functions of tabular display of data, construction of histograms with the ability to select parameters and subsequent data publication on the map of the Russian Federation. RESULTS:In Russia at the beginning of the 21st century, the characteristics of physical development of students living in diffe­rent constituent entities of the Russian Federation were established, which allowed us to talk about the presence of “regional scenarios” that must be taken into account when developing standards. At the age of 15, the range of average values of growth indicators for boys in the constituent entities ranged from 161.70±0.20cm in the Kursk Region to 174.55±0.40cm in the Krasnodar Territory (the difference was 12.85cm, p=0.001), for girls— from 154.60±0.50cm in the Republic of Tyva to 165.69±0.55cm in St.Petersburg (the difference was 11.09cm, p=0.001). The range of body weight of 15-year-old boys in the regions was from 50.17±0.70kg in the Kursk region to 66.00±0.80kg in the Khabarovsk region (difference 15.83kg, p=0.001), girls— from 46.90±0.60kg in the Chelyabinsk region to 58.40±0.70kg in the Khabarovsk region (difference 8.50kg, p=0.001). CONCLUSION:40regional standards for the physical development of the child population have been developed in a standar­dized manner, which allows taking into account regional characteristics and makes it possible to use them in practical healthcare in the constituent entities of the Russian Federation.

The structural organization of individual resources of the teacher's coping behavior

Introduction. All educators encounter stressful situations and experience negative emotions during their work, which necessitates the use of coping strategies to maintain personal well-being and uphold a high level of teaching quality. The contemporary state of the issue regarding the phenomenology of psychological resources for coping with stress is characterized by the dominance of an analytical research paradigm. This paradigm primarily focuses on individual, personal, and subject-activity characteristics as resources for coping with stress. The aim of this research is to determine the psychological structure of the coping behavior resources of educators. **Methods.** To conduct a comparative analysis of theoretical approaches to defining the resources of coping behavior, we employed conceptual content analysis, which allowed us to determine the frequency of mention of each resource for coping behavior. Subsequently, in accordance with the theoretical model, we selected diagnostic methods for these resources (26 psychodiagnostic techniques). The empirical study was conducted in educational institutions in the Kaluga region, Krasnodar Territory, Ulyanovsk, and Yaroslavl regions (Russian Federation). The study sample consisted of 901 individuals. Using exploratory factor analysis (principal component method, Varimax rotation), we identified the psychological structure of coping behavior resources. **Results.** In alignment with the theoretical model, we selected diagnostic methods for these resources, which allowed us to study both the resources themselves (their integral final values) and the components that make up these resources. The structure of coping behavior resources is formed by 114 variables, which were subsequently factor analyzed. The results of the factor analysis revealed that the psychological structure of coping behavior resources is represented by individual-psychological qualities, forming five blocks (existential, regulatory, emotional, socio-psychological, readiness for activity in uncertain situations). Factor 1 "Existential" (factor load - 24.1), Factor 2 "Readiness for Activity in Uncertain Situations" (14.9), Factor 3 "Regulatory" (13.1), Factor 4 "Socio-Psychological" (9.4), Factor 5 "Emotional" (7.5). **Conclusion.** There is a specific list of coping behavior resources that relevantly characterizes the specifics of a person's coping behavior in stressful situations. Psychological resources for coping behavior do not represent isolated psychological properties and states; instead, they form a holistic unity.

Congenital Epidermolysis Bullosa Epidemiology among Children of Russian Federation

Background. The prevalence of all types of congenital epidermolysis bullosa (СEB) worldwide is approximately 11 cases per 1 million according to the latest data from the American Epidermolysis Bullosa Registry. Data on the prevalence of СEB in Russian Federation is scattered, while data on life expectancy and mortality for this this disease is absent. This article presents medical and epidemiological data on children with СEB in Russian Federation. Objective. The aim of the study is to analyze clinical and epidemiological features of children with СEB in Russian Federation. Methods. We have performed analysis of the clinical and epidemiological features among pediatric population of Russian Federation with СEB using the “Registers of Genetic and Other Rare Diseases” of the “Butterfly Children” charitable foundation. Results. There are 491 children with СEB in Russian Federation as of 2024 according to the national registry data from “Registers of Genetic and Other Rare Diseases” of the “Butterfly Children” charitable foundation. The ratio of boys and girls was 1.08:1, that is relevant to the global data. The prevalence of CEB in children aged from 0 to 17 years in Russian Federation is 15.48 cases per 1,000,000 children as of January 1, 2024. The highest number of children with CEB were revealed in the Republic of Dagestan — 54 (11%) children, which is apparently due to the high rate of consanguineous marriages (50%). Other regions with high prevalence are Moscow Region (n = 28, 5.7%), Moscow (n = 25, 5.1%), Saint Petersburg (n = 26, 5.2%) and Krasnodar Territory (n = 23, 4.6%). Largest age group of children (from 12 to 18 years) includes 146 patients with mean age of 14.32 ± 1.72 years. The most common form of CEB is dystrophic one — 261 patients, the next one is simplex — 191 patients, then junctional form — 31 patient, and Kindler syndrome — 8 patients. The birth rate of children with CEB by year during the period from 2019 to 2023 (per 100,000 children born): 2019 — 1.42, 2020 — 2.09, 2021 — 2.65, 2022 — 2.76 and 2023 — 1.74. The arithmetic mean birth rate of children with CEB over a five-year period was 2.13 cases per 100,000 children born. The registry contains information on 22 deceased patients, average age was 3.06 ± 4.66 (from 0 to 15 years, median 0.54 years). The highest number of fatal outcomes was observed in the Republic of Dagestan (n = 3). Junctional CBE dominates in fatal outcomes among all the CEB types — 59.1% (n = 13 cases, 0.40 ± 0.22 years). The highest mortality was observed in I age group (from 0 to 1 year), which is 65.2% (15 fatal outcomes). Multisystem organ failure resulting from sepsis was the most common cause of death in both types of CEB (junctional and dystrophic). The mortality trend in the junctional form of CEB shows a decline in mortality, while the dystrophic type shows a stable situation during 2021–2023 years — 2 fatal outcomes per year. Junctional CEB has higher mortality rate at early age: survival curve shows sharp decline in the first months of life indicating high mortality in early life. The probability of survival drops to almost 0% in the first 100 days. Higher survival rate is more specific for the dystrophic type of CEB. Conclusion. This study demonstrates the significance and necessity to create and maintain registers for rare (orphan) diseases. Registry maintenance is an effective model for real understanding of the number of patients and value of needed assistance from the government to this category of patients.

Manifestations of emotional burnout in rehabilitation centers staff

Introduction. High emotional intensity of professional activities of rehabilitation centers staff, the need to constantly respond to unexpected non-standard pedagogical situations, the requirements for empathy and reflection in conditions of uncertainty, etc. determine the development of emotional burnout. Consequently, an employee suffering from emotional burnout cannot fulfill professional duties efficiently and solve tasks set by the organization. In this regard, it is necessary to understand the emotional sphere manifestations in employees of rehabilitation centers and organize appropriate preventive work. The purpose of this article is to summarize the data on studying the emotional peculiarities, to identify the manifestations of emotional burnout in rehabilitation centers employees. Materials and Methods. The methodological basis of the research includes studying the content of the phenomenon of emotional burnout with the focus on personal and professional self-fulfillment. In order to solve the research tasks the authors used analysis and generalization as theoretical methods and empirical methods, including testing, conversation, and methods of quantitative and qualitative data analysis. The sample consisted of 124 employees of rehabilitation centers in the Krasnodar Territory. Results. The article presents a theoretical overview and summarizes the results of studying the emotional sphere of rehabilitation centers employees. The study reveals the manifestations of emotional burnout, clarifies essential aspects of emotional disorders prevention. Conclusions. Emotional exhaustion, stress resistance disorders, tension, etc. are revealed as basic manifestations of emotional burnout in the research sample. The article concludes that prevention should be comprehensive and take into account the identified characteristics of emotional disorders.

История становления и развития Краснодарской научной медицинской школы анестезиологии и реаниматологии

Today, a relevant aspect of developing a competent understanding of medicine as a complex science interconnected with a number of humanities disciplines is to consider its history through the prism of the most outstanding research teams of scientific medical schools. The presented article is devoted to consideration of the historical path of formation and development of the scientific medical school of anesthesiology and resuscitation in Kuban. The authors provide data on the prerequisites (conditions for improving knowledge about anesthesiology and resuscitation in the region) and the main stage of this process. The materials of the historiographic research were data from archival funds, in particular the state archive of the Krasnodar Territory and the Museum of History of the Kuban State Medical University, personal collections of members of the scientific school, as well as specialized literature within the scope of the work. The research methodology involved the use of chronological, biographical, analytical and narrative methods.

Дополнение к лихенофлоре России IV. Bactrospora patellarioides и заметки по российским видам рода Bactrospora (Arthoniales, Ascomycota)

During the study of the lichen flora of the Utrish Nature Reserve (Abrau Peninsula, Krasnodar Territory), a Mediterranean-Atlantic lichen species Bactrospora patellarioides was identified for the first time for Russia and the Caucasus based on morphological and anatomical characteristics. This species grows on the trunk of Juniperus excelsa in the sub-Mediterranean xerothermic undisturbed pistacia-juniper community on the Black Sea coast. The species was previously known from the Mediterranean regions of Europe, Western Asia, North Africa, and is also known from Macaronesia and Western North America. Detailed description, habitat characteristics, ecology, and general distribution of species are presented. Notes on the genus Bactrospora are given, and a key to the Russian species (B. brodoi, B. dryina and B. patellarioides) is provided.

Method for disrupting the reproductive pheromone bond of the cotton bollworm on sunflower crops

Background: Synthetic analogs of sexual pheromones provide an effective method of biorational control of the number of phytophagous insects based on the directed disruption of reproductive functions in populations of the target pest on the protected crop without affecting the integrity of the agrocenosis. The work aims to determine the biological effectiveness of methods of mass trapping and disorientation of the cotton bollworm (Helicoverpa armigera) to protect sunflower (Helianthus annuus) crops.Methods: The experiments were carried out in 2022-2023 in the Central Zone of the Krasnodar Territory, Russia in the stationary scientific crop rotation at the Federal Research Center for Biological Plant Protection, Krasnodar (2.5 ha) and the Kononenko farm, Novovelichkovskaya village, Dinsky district (10 ha). The biological effectiveness of elimination and disorientation methods was judged by the degree of damage to plants and buds. The disorientation effect was calculated by the number of males captured at the disorientation sites compared to the control sites.Result: The decrease in males’ search activity (disorientation effect) reached 85.3%. The damage to buds by the pest in this variant was 2.0% compared to 10.8% in the control group. In the mass trapping variant, it equaled 1.5-3.0 and 7.5%, respectively, indicating the prospects of including these techniques in the H. armigera biorational control system.Conclusion: Our findings could aid future research on insect chemical communication to develop effective biological control methods.Keywords: Biological plant protection; Sunflower; Cotton bollworm; Synthetic sex pheromones; Elimination; Disorientation; Biological effectiveness

Studying the biochemical composition of peanut accessions from the VIR collection

Background. Peanut is one of the most important oil crops for food purposes. Natural peanut butter contains two unsaturated fatty acids, oleic and linoleic, which make up to 80% of the total fatty acid content in peanut oil. The quality of oil depends on the ratio between these two acids. Analyzing the diversity of oil content and fatty acid composition in peanut accessions preserved at VIR and assessing the effect of soil, climate, and other factors on these characters is vital for the development of new peanut cultivars rich in oil. Materials and methods. Peanut germplasm accessions were studied for their biochemical composition of fatty acids and the content of oil and protein. The accessions were reproduced for three years (2019–2021) at two ecogeographic locations: in Krasnodar Territory, and Astrakhan Province. Cv. ‘Otradokubansky’ was used as a reference. ANOVA was applied for statistical data processing. Results and discussion. The percentage composition of 18 fatty acids was calculated in peanut oil, with oleic and linoleic acids dominating. The content of oleic acid depended on the genotype for 42–53%, and that of linoleic acid, for 50–71%. The latter was also influenced by the place and year of cultivation. The ranges of variation over the three years of studies in Krasnodar Territory were 35.4–57.6% for oleic acid, and 18.3–38.1% for linoleic acid, whereas in Astrakhan Province they were 33.4–51.2%, and 30.9–42.7%, respectively. Under unfavorable conditions, the content of oleic acid in peanut prevailed. The oil content depended on the genotype for 81%, and its percentage was 32.0–44.4%.

Species diversity of pests of the suborder of shield bugs (Heteroptera) in agrocenoses of the Lazarevsky district of Sochi, Krasnodar Territory, Russia

Aim. The aim of the work was to study the species diversity of pests of the suborder of shield bugs (Heteroptera) in agrocenoses of the Lazarevsky district of Sochi.This paper presents data on the distribution, abundance and density of Heptoptera pest populations in this location. Heteroptera were gathered twice a month during the growing season (April to October) and weekly in the autumn‐winter period (November to March). The choice of registration date during the growing season was determined by the appearance of the first phytophagous individuals after wintering. Identification of the identified phytophages was carried out in the laboratories of the station according to generally accepted methods.The paper presents data on the distribution, abundance and density of pest populations of the suborder of shield bugs (Heteroptera) in the Lazarevsky district of Sochi, Krasnodar Territory. As a result of this research, the species composition of the most common species of bedbugs in this area has been revealed. The most harmful species noted were: berry shield Dolycoris baccarum Linnaeus, 1758; ornate shield Eurydema ornata Linnaeus, 1758; Green shield Nezara viridula Linnaeus, 1758; ruled shield Graphosoma lineatum Linnaeus, 1758; brown‐marbled bug Halyomorpha halys Stål, 1855; oak lace‐maker Corythucha arcuata Say, 1832; sycamore lace‐maker Corythucha ciliata Say; pear lace‐maker Stephanitis pyri Fabricius and linden bug Oxycarenus lavaterae Fabricius, 1787.As a result of the research undertaken, an increase in the total number of bedbug populations, an expansion of trophic connections, an increase in aggressiveness and a change in the status of individual pest species were revealed.

Index of development of transport infrastructure as a factor of sustainable development of the coastal zone of the Sea of Azov, Russia

As a zone of interaction between land and sea, coastal zones are becoming a strategic area for achieving a sustainable expansion of economic space. The object of study is the coastal zone of the Sea of Azov (Rostov Region and Krasnodar Territory). The subject of the study is an assessment of the dynamics of transport infrastructure development index as a factor in the sustainable development of the coastal zone of the Sea of Azov.Methodological approaches to assessing the sustainable development of the territory were based on a methodology based on methods of system analysis, mathematical modeling, and GIS technologies. An indicator has been formulated that considers the area of the territory, the population, the length of public roads of local importance, the shipment of goods of local production, the work and services performed on their own, in order to measure the ‘economic’ load on the transport infrastructure.The analysis of the state of the transport infrastructure of the regions of the coastal zone for the period from 2015 to 2021 was carried out. Based on an analysis of the transport infrastructure index, it was concluded that the attraction of municipalities to the Rostov agglomeration, together with the strengthening of social, trade and infrastructure contacts with megacities will lead to a further increase in the anthropogenic load on the territory. The areas studied of the Krasnodar Territory belong to the Northern Economic Zone – a territory with a diversified economy, characterised by cross‐border links with neighboring regions.Highways are the most important link in the transport and logistics system. The level of development and the technical condition of the road network have a significant and diverse impact on the economic and social development of both the state as a whole and it individual regions.

Evaluation of oat accessions from different ecological groups in the steppe environments of Krasnodar Territory

Background. Studying genetic diversity and identifying sources of valuable agronomic traits for specific environmental conditions is of great importance for plant breeders when they select source material for crop improvement. Materials and methods. Three hundred and seven oat accessions of various ecogeographic origin were tested under the conditions of the Kuban Experiment Station, a branch of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), in 2014–2019. Cv. ‘Valdin 765’ served as the reference. Weather conditions across the years of the study were contrasting in terms of the hydrothermal regime. Results. The inter-cultivar and year-to-year variability of the oat collection was analyzed in Krasnodar Territory and accessions promising for breeding were identified for a set of useful agronomic traits. The most significant differences among the studied oat accessions were recorded for the time of ripening and panicle characteristics. High yield of an accession under the conditions of Krasnodar Territory was associated with its high 1000 grain weight, which positively correlated with the duration of the period from panicle emergence to ripening and negatively with the length of the panicle, number of grains per panicle, plant height, and duration of the periods from germination to panicle emergence and from germination to ripening. Conclusion. The study of useful agronomic traits resulted in identifying oat accessions with the yield exceeding the reference (cv. ‘Valdin 765’) by 114–135%, that ripened 6–8 days earlier than the reference, were resistant to lodging, had short stems, manifested combined resistance (9 points) to crown and stem rust, low filminess (< 25%), good leafiness, high oil content (> 6%), increased protein content (> 14%), and high levels of yield structure components.

Diversity of Northwestern and North Caucasian Populations of <i>Puccinia striiformis</i> f. sp. <i>tritici</i> by Virulence and Microsatellite Loci in 2022

Yellow rust (Puccinia striiformis f. sp. tritici, Pst) is a potentially dangerous disease of wheat. Genetic protection of wheat is an environmentally safe method of control. For its successful application, information on the structure of regional populations of the pathogen is needed. The purpose of these studies is to characterize the virulence and molecular polymorphism of Russian Pst populations in 2022. Wheat leaves with Pst urediniopustules were obtained from the North Caucasus (Krasnodar Territory, Dagestan, Kalmykia) and Northwestern (Leningrad region) regions. Virulence analysis was performed on 14 isogenic lines (AvocetNIL) and 15 differentiator varieties. The polymorphism of 20 microsatellite loci was evaluated in molecular analysis. We used SSR markers recommended by the Global Rust Reference Center. Virulence was studied in 74 monopustular isolates: 29 Dagestan, 10 Krasnodar, 5 Kalmyk, 30 northwestern. Resistance to all isolates was shown for lines with genes Yr5, Yr10, Yr15, Yr24, Yr26 and varieties Moro (Yr10, YrMor) and Nord Desprez (Yr3, YrND, Yr+). Isolates virulent to the AvYr17 line were detected for the first time in Dagestan and Krasnodar populations. They were moderately represented in the northwestern population (13%). Significant variation was observed in lines and varieties with the Yr1 and Yr3 genes. There was a decrease in virulence to Yr7 and YrSp compared to 2019–2021. 28 phenotypes (races) were determined in the virulence analysis (15 in Dagestan, 11 in North West, 3 in Krasnodar and 2 in Kalmyk). A common phenotype was noted for three North Caucasian Pst samples. The genetic distances between the identified phenotypes were estimated. In the multidimensional diagram, most of them combined together in a common group, with the exception of three Dagestan phenotypes with the fewest virulence alleles. According to the Fst index, Dagestan and Kalmyk Pst collections were characterized by high similarity; others differed moderately from them. The long-term virulence dynamics (2019–2022) of Pst populations in the Northwestern and North Caucasian regions was assessed. High similarity was determined between all regional population samples in 2019 and 2020. The northwestern and Dagestan populations differed slightly from them in 2021 and 2022. In 2022, the Krasnodar and Kalmyk populations were divided into separate groups, which differed from each other and from the main group. Long-term results of virulence analysis indicate a high dynamic of the structure of Pst populations in Russia. All North Caucasian isolates and 23 Northwestern isolates were used in the SSR analysis. Six loci (RYN3, RYN9, RYN12, WU6, RJO21, RJO24) were monomorphic. Three polymorphic alleles were identified in the RYN13 and RJO27 loci and two in the remaining studied ones. Significant deviations from the Hardy-Weinberg equilibrium are noted for most loci. The observed heterozygosity exceeded the expected one, which indicates an excess of heterozygotes and the clonal origin of the population. The studied collection of isolates was represented by 20 multilocus genotypes (MGs) (Dagestan and Northwestern – 11 each, Krasnodar – 3, Kalmyk – 1). Common genotypes were detected in Dagestan, Krasnodar and Northwestern populations (MG_1); Dagestan, Kalmyk and Northwestern (MG_2); Dagestan and Krasnodar (MG_3, MG_4). The genetic distances between MGs were estimated. In the multidimensional diagram, they are divided into 4 groups. The main group included 80% of MGs. One Dagestan MG, two Northwestern MG and MG_3 common for Dagestan and Krasnodar collections, significantly differentiated from the main group and among themselves. According to the Fst index, most regional Pst collections were moderately differentiated among themselves, with the exception of Dagestan and Kalmyk, which is consistent with the virulence analysis. According to the Mantel test, a moderate correlation was found between the results of virulence analysis and SSR (r = 0.6). This indicates that both analyses can be used in assessing the genetic polymorphism of Pst. The high variability of Russian populations based on virulence and microsatellite loci determines the need for annual monitoring of regional Pst populations in Russia.

Variability of Morphological Characters of the Genus Alburnoides (Pisces, Leuciscidae) from Watercourses in Southern Russia

The variability of the morphological characters of the genus Alburnoides Jeitteles, 1861 from watercourses in the south of Russia (five rivers of the Crimean Peninsula and two rivers of the Krasnodar Territory) is considered. It has been established that in spirlins within the study areas, body sizes average from 60.1 ± 1.45 mm in individuals from the river Gostagayka (Krasnodar region) up to 88.2 ± 1.87 mm in fish from the river Angara (Crimean Peninsula). Using the Mann–Whitney test, statistically significant differences were registered between the samples in most plastic characters; they were absent in meristic characters. In the studied regions, heterogeneity in the structure of the genus Alburnoides was noted: according to the results of cluster analysis for the totality of all studied characters, the greatest similarity was found in samples from Crimean rivers that are geographically close to each other (rivers Chernaya, Belbek, Kacha and Alma), but group from the river Angara adjoins them at a higher level of divergence. A separate group is formed by samples from the rivers of the Krasnodar Territory. The obtained data are consistent with the literary information on the formation of the ichthyofauna in the rivers of Crimea; the established clear differences between the charaters from the rivers of southern Russia require a more detailed study.

Germination of soybean seeds after low-temperature storage and its dependence on weather and climate conditions in the places of reproduction of accessions

Background. Soybean (Glycine max (L.) Merr.) is a microbiotic whose seeds quickly lose germination in uncontrolled conditions at room temperatures. This determines the need to preserve accessions in special conditions. Low temperature storage (LTS) allows to maintain high seed germination ability, but not for all accessions. Therefore, it is relevant to assess the results of LTS at the VIR Genebank and to make an attempt to determine the best geographical conditions for growing plants and harvesting seeds that will be subjected to LTS. Materials and methods. The work was carried out on 312 soybean accessions from the VIR collection, originating from almost the entire area of soybean cultivation. The accessions used in the research were reproduced at three VIR experiment stations (ES), namely Adler ES, Kuban ES (Krasnodar Territory) and the Far East ES (Primorsky Territory). The seeds were harvested in 1999 to 2017 and stored in sealed foil laminated bags at –10°C from 2002 to 2021. The accessions were removed from LTS and germination assessed in 2022. Results. At the beginning of LTS, seed germination (Gi) ranged from 12 to 100% (averaging 79.1%), while that after LTS (Gr) ranged from 1 to 97% (57.8% on average). The retention of germination ability was expressed as the germination index (GI = Gr / Gi), which ranged from 0.02 to 1.73 (an average of 0.72). All the indicators of germination in the range of up to 20 years of LTS were not associated with the duration of LTS and with the number of years before LTS (0.5-4 years in our research). The climatic conditions characteristic of the place of reproduction had a significant impact on Gi and Gr, but not on GI. The average Gi of seeds obtained at the Adler ES and Kuban ES did not differ significantly (77.4% and 75.7%), while that of seeds harvested at the Far East ES was significantly higher (84.0%). Gr of the accessions from the Far East ES (64.3%) was also greater than that of accessions from the Kuban ES (52.2%) and Adler ES (57.2%). The excessively high sum of temperatures above 10°C (recorded in some years at all stations) reduced Gi, Gr but had little effect on GI. The highest values of Gi, Gr, and GI were demonstrated by the accessions maturing in 101-120 days. Additionally drying of the most late-ripening accessions (later than 140 days) in sheaves under an awning yielded seeds with high germination ability and good LTS tolerance. Conclusion. The seeds from different places of reproduction of accessions differed in germination before and after LTS, and, on an average, the highest values were demonstrated by the seeds harvested at the Far East ES, where the temperature regime is closer to the optimal one for soybeans. The seed germination index showed relative independence from the conditions of reproduction of accessions, including the climate and weather conditions, as well as the initial germination ability and LTS duration.

«Завоевать и уничтожить»: идеология фашистского оккупационного режима на территории Краснодарского края (июль 1942 г. – октябрь 1943 г.)

The article explores the German occupation rule in the Krasnodar Territory, which was formed between the summer of 1942 and the fall of 1943. The Fascists established a civil and military administrative machinery with the goal of financially looting the territory and physically destroy-ing the civilian population. When we examine the occupying regime’s ideology, we see that it is anti-human. Wehrmacht soldiers, special formations of the German Army, and traitors among lo-cal citizens were all direct participants in crimes against humanity and extermination of civilians. Mass executions of people occurred within the area of the Krasnodar area, which included the Adygea Autonomous Region. Archival sources reveal the reality of the population’s planned elim-ination, which included children and women. The atrocities of the Wehrmacht are documented in the acts of the Extraordinary State Commission for the Establishment and Investigation of the Atrocities of the Nazi Invaders and their Companions, which were compiled in all districts and cities throughout the region. Many of those who committed crimes were punished during the war, and this practice persisted after it ended.

Метано- и сульфидогенез в озерах полуострова Абрау (Краснодарский край)

The geographical position, tectonic and geological-hydrogeological structure of the basin of the Malyy Liman and Abrau lakes, their landscape features as natural monuments of regional importance located on the territory of the Abrau peninsula of the Krasnodar Territory are described. Using the example of the aquatic landscapes of the Malyy Liman and Abrau lakes and the river of the same name, water and bottom sediments were studied. Hydrochemical parameters, the content of methane and sulfide sulfur in bottom sediments and methane in water as components of aquatic landscapes were studied. The results of the determination of sulfides, as well as the characteristic odor and color of bottom sediments, indicate the presence of free H2S and hydrotroilite (FeS ∙ nH2O) in them. Electron microscopy revealed the presence of hollow spheres in the bottom sediments, which indicates their possible gas saturation. Microinclusions of biogenic pyrite (FeS2), which was formed on fragments of plant and organism residues, were found under an electron microscope. In aerobic-anaerobic conditions of the upper layer of bottom sediments of lakes, pyrite formation occurs both directly through a one-stage process of pyritization of reactive iron, but also through a multi-stage process of pyrite formation from iron monosulfide. It has been established that lakes are exposed to anthropogenic influences, which can increase many times during the holiday season. This leads to their eutrophication and increased meanogenesis, which in turn contributes to the activation of methane emissions into the atmosphere. Specific methane fluxes from the water surface of the Malyy Liman and Abrau lakes have been determined.

The First Cranial Trepanation Find in a Meotian Burial Ground (Starokorsunskiy Settlement No. 2, Krasnodar Region)

A young woman’s skeleton dating back to the Meotian archaeological culture was studied during the investigation of the paleoanthropological series of the burial ground of the Starokorsunsky settlement No. 2. The burial ground is located in the Krasnodar Territory and is dated to the 4th century BC – 2nd century AD, while the burial under discussion dates back to the 2nd century BC – 2nd century AD. A trepanation hole with signs of healing was found on the skull of a young female of 17–25 years of age. In order to describe the pathological changes recorded on the cranial vault recommendations applied in modern paleoanthropology were used. Differential diagnosis was performed using descriptive macroscopic and X-ray diagnostic methods. It was possible to establish that the trepanation hole was made at least several years before the death of the individual. We suggested that the reason for the operation could be early synostosis of the skull sutures, and this manipulation was designed to reduce intracranial pressure and headaches caused by it. The article discusses the likely origins of medical skills for surgical manipulation. It is concluded that so far the data obtained do not allow us to speak unequivocally about the acquisition of medical knowledge by Meots from residents of the Asian Bosporus or from the Sarmatian environment.

Features of Teaching First Aid Skills to People without Medical Education

A comparative study was conducted on the effectiveness of various methods of training law enforcement officers of the Krasnodar Territory in providing first aid, taking into account the specific conditions of official activity.