This paper presents the results of thermal-hydraulic calculation of “Steam Line Break” analysis at full power reactor for VVER-1000/V320 units at Kozloduy Nuclear Power Plant (KNPP), done during the development of symptom based emergency operating procedures (SB EOPs) for this plant. The RELAP5/MOD 3.2 computer code has been used in performing the analyses in a VVER-1000 Nuclear Power Plant (NPP) model. A model of VVER-1000 based on Unit 6 of Kozloduy NPP has been developed for the systems thermal-hydraulics code RELAP5/MOD 3.2 at the Institute for Nuclear Research and Nuclear Energy–Bulgarian Academy of Sciences (INRNE–BAS), Sofia.The main purpose of the analysis is to estimate the parameters of the monitored plant which are used to identify symptoms that are used by operators to identify the plant's state and the critical safety function (CSF). The results of the thermal-hydraulic analyses have been used to assist KNPP specialists in analytical validation of EOPs.The performed analysis is based on a previously used bounding approach in analytical validation of SB EOPs. Based on this approach a list of scenarios has been performed, involving a different number of safety systems with or without operator actions. The presented thermal-hydraulic calculations of the accident scenarios involve the loss of CSF “Subcriticality” for VVER-1000/V320 units at KNPP.