Korean Wheat Research Articles

Mapping of QTL for Pre-Harvest Sprouting Resistance and Seed Dormancy in a Doubled Haploid Population

Pre-harvest sprouting (PHS) of wheat reduces grain yield and has deleterious effects on wheat flour quality. PHS resistance is strongly associated with grain color and seed dormancy. Resistance to PHS is a priority of wheat breeding program. We have studied a population of 122 Doubled Haploid (DH) lines from the cross between two cultivars, 'Keumkang' and 'Olgeuru', in order to detect QTLs correlated with PHS resistance. PHS was evaluated using mist spray in the humid chamber (PHS-MS), and the germination index (GI) was determined for two years. The variety 'Keumkang' has white grains and higher percentage of PHS rate (30.2%), while 'Olgeuru' has red grains and lower PHS rate (2.8%). There was significant difference in grain color according to the GI. PHS-MS was positively correlated with GI (r = 0.732, P < 0.001). Two molecular markers, Xbarc12 and Xgwm3, were found correlated with PHS resistance and GI. These markers were mapped on chromosomes 3A and 3D with LOD scores of 4.95 and 4.02, respectively. Xbarc12 explained the highest amount of phenotypic variation (R 2 ) which is 11.99% in GI and 27.25% in PHS. Xgwm3 explained 9.10% of phenotypic variation in GI. The respective value of PHS-MS and GI in Korean wheat cultivars ranged from 1.37% to 86.75% and from 0.03 to 0.73. This study provides useful information for marker-assisted wheat breeding for PHS resistance improvement in Korea.

Bran Characteristics and Bread‐Baking Quality of Whole Grain Wheat Flour

ABSTRACTVariations in physical and compositional bran characteristics among different sources and classes of wheat and their association with bread‐baking quality of whole grain wheat flour (WWF) were investigated with bran obtained from Quadrumat milling of 12 U.S. wheat varieties and Bühler milling of six Korean wheat varieties. Bran was characterized for composition including protein, fat, ash, dietary fiber, phenolics, and phytate. U.S. soft and club wheat brans were lower in insoluble dietary fiber (IDF) and phytate content (40.7–44.7% and 10.3–17.1 mg of phytate/g of bran, respectively) compared with U.S. hard wheat bran (46.0–51.3% and 16.5–22.2 mg of phytate/g of bran, respectively). Bran of various wheat varieties was blended with a hard red spring wheat flour at a ratio of 1:4 to prepare WWFs for determination of dough properties and bread‐baking quality. WWFs with U.S. hard wheat bran generally exhibited higher dough water absorption and longer dough mixing time, and they produced smaller loaf volume of bread than WWFs of U.S. soft and club wheat bran. WWFs of two U.S. hard wheat varieties (ID3735 and Scarlet) produced much smaller loaves of bread (&lt;573 mL) than those of other U.S. hard wheat varieties (&gt;625 mL). IDF content, phytate content, and water retention capacity of bran exhibited significant relationships with loaf volume of WWF bread, whereas no relationship was observed between protein content of bran and loaf volume of bread. It appears that U.S. soft and club wheat bran, probably owing to relatively low IDF and phytate contents, has smaller negative effects on mixing properties of WWF dough and loaf volume of bread than U.S. hard wheat bran.

빵과 면의 가공적성 증진을 위한 밀 저장단백질 Glu-1Dy10을 발현하는 마커프리 형질전환 벼 개발

Rice flour is used in many food products. However, dough made from rice lacks extensibility and elasticity, making it less suitable than wheat for many food products such as bread and noodles. The high-molecular weight glutenin subunits (HMW-GS) of wheat play a crucial role in determining the processing properties of the wheat grain. This paper describes the development of marker-free transgenic rice plants expressing a wheat Glu-Dy10 gene encoding the HMG-GS from the Korean wheat cultivar ‘Jokyeong’ using Agrobacterium-mediated co-transformation. Two expression cassettes, consisting of separate DNA fragments containing Glu-1Dy10 and hygromycin phosphotransferase II (HPTII) resistance genes, were introduced separately into Agrobacterium tumefaciens EHA105 for co-infection. Each EHA105 strain harboring Glu-1Dy10 or HPTII was infected into rice calli at a 3: 1 ratio of Glu-1Bx7 and HPTII. Among 290 hygromycin-resistant T0 plants, we obtained 29 transgenic lines with both the Glu-1Dy10 and HPTII genes inserted into the rice genome. We reconfirmed the integration of the Glu-1Dy10 gene into the rice genome by Southern blot analysis. Transcripts and proteins of the Glu-1Dy10 in transgenic rice seeds were examined by semi-quantitative RT-PCR and Western blot analysis. The marker-free plants containing only the Glu-1Dy10 gene were successfully screened in the T1 generation.

Diminution of mycotoxins from Fusarium sp. in barley and wheat through post-harvest processing methods

The objective of this study was to analyze mycotoxin contents in Korean barley and wheat infected with Fusarium sp. The major contaminant was determine among deoxynivalenol, nivalenol (NIV), and zearalenone, as well as diminution rate of mycotoxin contents by milling, washing, and boiling processes. NIV was found as a major mycotoxin contaminant in Korean barley and wheat, and bran showed higher contamination level than the inner part in whole infected cereal. The results indicate that the milling process of the diseased barley and wheat showed dramatic diminution rate of 84.4%. Furthermore, the washing on barley reduced mycotoxin infection up to 81.0%, and boiling showed 82.7% diminution effect.

Quality Characteristics of Korean Wheat Bread Prepared with Substitutions of Naturally Fermented Rice Starters

Quality Characteristics of Korean Wheat Bread Prepared with Substitutions of Naturally Fermented Rice Starters

Quality Characteristics of Korean Wheat Bread Prepared with Substitutions of Naturally Fermented Rice Starters

Quality Characteristics of Korean Wheat Bread Prepared with Substitutions of Naturally Fermented Rice Starters

지역별 국산밀 재배 현황 및 원맥 특성 평가. I. 국산밀 재배 농가의 지역별 재배 현황 조사

국산밀을 재배하는 전국 175농가를 대상으로 2년 동안 재배 현황에 대한 자료를 수집 분석한 결과, 평균 재배 경력과 재배면적은 7.7년과 2.4~3.3 ha였으며, 재배 품종은 금강밀이 주로 재배되었고 조경밀과 백중밀이 남부지역에서 재배 면적이 늘어나고 있다. 파종은 대부분 농가에서 10월 하순에서 11월 상순에 휴립광산파로 이루어지고 있으며, 시비는 2종 복합비료를 이용하여 평균 29.7 kg/10a이상 기비로 시용하였으며, 추비는 2월 하순~3월 상순에 질소비료를 1회 시용하는 농가가 주를 이루었는데, 농가 현장에서 표준시비량에 비해 과량의 비료가 시용되고 있기 때문에 적정 시비에 대한 관리가 필요하다. 재배 밀의 출수는 기상 영향으로 2011년은 5월 상순, 2012년은 4월 하순에 이루어졌으며, 밀 수확은 대부분 농가에서 6월 중순에 이루진 것으로 나타났다. 재배 면적 확대와 밀 재배 농가 소득 향상을 위하여, 수발아 및 붉은곰팡이병 저항성 증진과 기상이변에 대응할 수 있는 재해저항성 증진 품종 개발이 이루어져야하며, 국산밀 품질 향상을 위한 보급종 생산 및 확대가 필요하며, 지역별 밀 재배 농가의 규모화에 대한 지원과 재배 관리 체계 확립과 현장 지원 확대가 필요하다. The cultivation situation of Korean wheat of 175 farmers in nationwide for two years, 2010/2011 and 2011/2012, was analyzed to obtain basic data for extension cultivated area and enhancing the self-sufficiency ratio of Korean wheat. Compared to the mean temperature and precipitation in the normal year, the mean temperature was lower before the heading stage and higher amount of precipitation after the heading stage in 2010/2011 and higher the mean temperature and lower amount of precipitation after the heading stage in 2011/2012. Average cultivation career and area were 7.7years and 2.4~3.3ha, Keumkang cv. was mainly cultivated for two years and Jokyung and Baekjoong cvs. were increased cultivation areas in southern part of Korea, Gyeongsangnam-do, Jeolllanambuk-do and Kwangju metropolitan city, including in 2011/2012. Most farmers (144) sown wheat seeds from late October to the beginning November with broadcasting method and the other famers were sown using the drill method. Average amount for basal fertilizer was 29.7 kg/10a with complex fertilizer mixed for wheat and barley cultivation, which was higher amount compared to recommended rate of fertilizer amount by rural development administration. Top dressing using nitrogen fertilizer was applied from in the late February to the beginning March. Heading date was the beginning May in 2011 and the late April in 2012, which the mean temperature from regeneration stage to tillering stage in 2011 was higher than that of 2012. Most farmers harvested wheat in mid-June and Pre-harvest sprouting and Fusarium head blight were occurred in 2011 due to the high amount precipitation during grain filling period.

종실과 밀가루 특성이 국수 면대 색깔에 미치는 영향과 철 함량 간이 검정

ABSTRACT This study was carried out to characterize the color of noodle sheet made from Korean wheat cultivars, which were then evaluated on grain and flour properties, in order to enhance the breeding-line selection for noodle wheat. In particular, a rapid method was applied to investigate the effects of iron (Fe) contents on the lightness of noodle sheet for the selection of breeding-lines with high Fe contents. Wheat flour lightness of Korean wheat cultivars had a negative correlation with ash (r = -0.634, P<0.01), protein (r = -0.635, P<0.01) and total polyphenols (r =-0.493, P<0.05). The noodle sheet color showed a positive correlation with lightness, but a negative correlation with test weight, 1000 kernels weight, PPO activity and total polyphenol contents. Also, upon the wheat flour properties, the ash, protein and total polyphenol contents had a negative correlation with the lightness of noodle sheet, and this relation varied depending on the year and cultivars. The Fe content had a negative relation with the color of wheat flour and the lightness of noodle sheet, and the potassium hexacyanoferrate(II) trihydrate (PHT) test resulted in blue color. There was no correlation between Fe contents and the color of grains with PHT treatment, but after grounding the whole grains, a negative correlation was found between Fe contents and redness (r = -0.665, P<0.001) and yellowness (r = -0.658, P<0.001) of whole wheat flour with PHT treatment. On the other hand, the lightness of wheat flour with PHT treatment showed a negative relation with Fe content. Keywords : wheat, noodles, color, Fe

A Simple Method for the Assessment of Fusarium Head Blight Resistance in Korean Wheat Seedlings Inoculated with Fusarium graminearum.

Fusarium head blight (FHB; scab) caused mainly by Fusarium graminearum is a devastating disease of wheat and barley around the world. FHB causes yield reductions and contamination of grain with trichothecene mycotoxins such as deoxynivalenol (DON) which are a major health concern for humans and animals. The objective of this research was to develop an easy seed or seedling inoculation assay, and to compare these assays with whole plant resistance of twenty-nine Korean winter wheat cultivars to FHB. The clip-dipping assay consists of cutting off the coleoptiles apex, dipping the coleoptiles apex in conidial suspension, covering in plastic bag for 3 days, and measuring the lengths of lesions 7 days after inoculation. There were significant cultivar differences after inoculation with F. graminearum in seedling relative to the controls. Correlation coefficients between the lesion lengths of clip-dipping inoculation and FHB Type II resistance from adult plants were significant (r=0.45; P<0.05). Results from two other seedling inoculation methods, spraying and pin-point inoculation, were not correlated with adult FHB resistance. Single linear correlation was not significant between seed germination assays (soaking and soak-dry) and FHB resistance (Type I and Type II), respectively. These results showed that clip-dipping inoculation method using F. graminearum may offer a real possibility of simple, rapid, and reliable for the early screening of FHB resistance in wheat.

Relationship between pre-harvest sprouting and functional markers,TaVp-1A, TaSdr-B1andTaPHS1in Korean wheat cultivars

Pre-harvest sprouting (PHS) of Korean wheat cultivars was evaluated for five years and allelic variations of functional markers associated with seed dormancy and PHS, TaVp-1A, TaSdr-B1 and TaPHS1, were also evaluated in this study to improve PHS resistance in Korean wheat breeding programmes. PHS resistance was evaluated using mist spray in humid chamber (PHS-MS) over five years. PHS-MS were significantly influenced by year, genotype and the interaction between year and genotype. PHS-MS ranged from 1.57 to 84.38%. Red-grained wheat cultivars showed lower PHS-MS (11.39%) than white-grained cultivars (62.24%). There was no significant difference in PHS value of Korean wheat cultivars carried different TaVp-1A alleles although five alleles were found in Korean wheat cultivars. The frequency of TaSdr-B1a and TaSdr-B1b allele in Korean wheat cultivars was 79.2 and 20.8%, respectively. TaSdr-B1a allele showed lower PHS value (16.50%) than TaSdr-B1b allele (52.99%). For TaPHS1 allele, there were two types of haplotypes I and II in Korean wheat cultivars. There was non-significant difference of PHS resistance in Korean wheat cultivars carried between haplotype I (27.19%) and haplotype II (20.45%).

Mapping of quantitative trait loci (QTL) for spike length and kernel number in recombinant inbred lines (RILS) population derived from wheat line with large kernel number

This study was conducted to identify the quantitative trait loci (QTL) associated with spike length and kernel number by analysis of 122 recombinant inbred lines (RILs) derived from a cross between Korean wheat cultivar and Iksan 370, which large kernel number line carried with the longer spike length and higher kernel number/spike than Korean wheat cultivars. Spike length, kernel number/spike and spike density were investigated from replicated field trials in 2013–14 at upland condition in Iksan, Korea. A genetic map was constructed with 92 microsatellite marker loci and a total of nine QTL were identified on six chromosomes for spike length and kernel number. The three QTL, Xwmc 44, Xbarc 205 and Xgwm 292 were detected on chromosomes 1B and 5D, and explained 9.7–16.5% of phenotypic variation for spike length. Among these QTL, Xwmc 44 and Xgwm 292 also explained 8.2 and 10.8% of phenotypic variation for spike density and kernel number/spike, respectively. Xgwm 135, located on chromosomes 1A, was accounted for 10.9% of phenotypic variation for kernel number/spike. Other two QTL for spike density, Xgwm 495 and Xwmc 285, were mapped on chromosome 4B and 4D, respectively. These QTL explained 6.9–7.9% of phenotypic variation for spike density. Three QTL, Xwmc 44, Xgwm 292 and Xgwm 135 for spike length, kernel number/spike and spike density were applied to validate relationship between genotypes of QTL and 29 Korean wheat cultivars grown for four years on upland conditions. Korean wheat cultivars carried aaa and bbb genotypes based on the order of these three QTL, higher and lower kernel number/spike and spike density than other genotypes, although there was not a significant difference in spike length. These QTL could be used in marker assisted selection wheat lines with higher kernel numbers in Korean wheat breeding programmes.

Effects of Agronomic Characteristics and Grain Morphology on Pre-harvest Sprouting in Korean Wheat Cultivar

Effects of Agronomic Characteristics and Grain Morphology on Pre-harvest Sprouting in Korean Wheat Cultivar

In vitro Evaluation of Different Feeds for Their Potential to Generate Methane and Change Methanogen Diversity.

Optimization of the dietary formulation is the most effective way to reduce methane. Nineteen feed ingredients (brans, vegetable proteins, and grains) were evaluated for their potential to generate methane and change methanogen diversity using an in vitro ruminal fermentation technique. Feed formulations categorized into high, medium and low production based on methane production of each ingredient were then subjected to in vitro fermentation to determine the real methane production and their effects on digestibility. Methanogen diversity among low, medium and high-methane producing groups was analyzed by PCR-DGGE. The highest methane production was observed in Korean wheat bran, soybean and perilla meals, and wheat and maize of brans, vegetable protein and cereal groups, respectively. On the other hand, corn bran, cotton seed meal and barley led to the lowest production in the same groups. Nine bacteria and 18 methanogen 16s rDNA PCR-DGGE dominant bands were identified with 83% to 99% and 92% to 100% similarity, respectively. Overall, the results of this study showed that methane emissions from ruminants can be mitigated through proper selection of feed ingredients to be used in the formulation of diets.

밀 고분자 글루테닌 유전자를 이용하여 빵 가공적성 증진을 위한 마커 프리 형질전환 벼의 개발

High-molecular weight glutenin subunits (HMW-GS) have been shown to play a crucial role in determining the processing properties of the wheat grain. We have produced marker-free transgenic rice plants containing a wheat Glu-1Bx7 gene encoding the HMG-GS from the Korean wheat cultivar ‘Jokyeong’ using the Agrobacterium-mediated co-transformation method. The Glu-1Bx7-own promoter was inserted into a binary vector for seed-specific expression of the Glu-1Bx7 gene. Two expression cassettes comprised of separate DNA fragments containing only Glu-1Bx7 and hygromycin phosphotransferase II (HPTII) resistance genes were introduced separately to the Agrobacterium tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring Glu-1Bx7 or HPTII was infected to rice calli at a 3:1 ratio of Glu-1Bx7 and HPTII, respectively. Then, among 216 hygromycin-resistant T0 plants, we obtained 24 transgenic lines with both Glu-1Bx7 and HPTII genes inserted into the rice genome. We reconfirmed integration of the Glu-1Bx7 gene into the rice genome by Southern blot analysis. Transcripts and proteins of the wheat Glu-1Bx7 were stably expressed in the rice T₁ seeds. Finally, the marker-free plants harboring only the Glu-1Bx7 gene were successfully screened at the T₁ generation.

국산밀 품질 향상을 위한 지역별 재배 현황 및 원맥 특성 평가 II. 농가 수집 원맥 특성 평가

국산밀을 재배하는 전국 175농가를 대상으로 2년 동안 농업형질과 원맥 특성을 조사 한 결과, 간장, 수장, 경수와 이형주율은 년차간 차이를 나타내었다. 시비량이 많았던 남부지역에서 재배된 밀의 간장이 중북부 지역 보다 길었으며, 추비시기는 간장에 영향이 없었지만, 3월 중순 이후에 늦게 추비를 준 농가의 수장은 다른 농가에 비하여 길었으나 경수는 감소하였다. 추비 횟수는 농업형질에 영향을 미치지 않는 것으로 나타났다. 농업형질과 마찬가지로 원맥 특성도 연차간 변이가 있었으며, 남부지역에서 재배된 밀은 중북부지역에서 재배된 밀에 비하여 천립중이 높았으며, 리터중은 낮았으며, 전라북도는 다른 지역에 비하여 낮은 회분 함량과 높은 단백질 함량을 나타냈으며, 전라남도는 다른 지역에 비하여 높은 회분 함량을 나타내었다. 추비시기가 리터중에는 영향을 주었지만 천립중과 회분 및 단백질 함량에는 영향을 주지 않았으며, 추비 횟수도 원맥 특성에 영향이 없는 것으로 나타났다. 시비량에 따라 간장이 증가하고, 천립중과 단백질 함량이 증가하였으며, 수장과 회분함량은 시비량과 부의 상관을 나타내었다. 단백질 함량은 천립중과 정의 상관을 나타내었지만 회분 함량은 천립중 및 리터중과 부의 상관을 나타내었다. 간장이 증가할수록 천립중은 증가하고 리터중은 감소하였으며, 수장은 천립중과 부의 상관을 나타내었다. 시비량이 간장 및 원맥 특성에 영향을 미치기 때문에 품종별 용도에 맞는 밀 품종을 재배하기 위해서는 적정 시비기술에 대한 농가 지도가 필요하다. 또한, 국산 밀의 생산성 증진과 품질 향상 및 균일성 확보를 위해서는 용도별 가공적성에 적합한 품종에 대한 집단 재배단지를 조성하고 철저한 생산 관리와 체계적인 수확 후 관리 구축에 대한 종합적인 고려가 있어야 할 것이다. Agronomic characteristics and grain properties of Korean wheat of 175 farmers in nationwide for two years, 2010/2011 and 2011/2012, were evaluated to support basic data for improving grain quality of Korean wheat and enhancing Korean wheat consumption. Agronomic characteristics, including culm length, spike length, number of <TEX>$spike/m^2$</TEX> and rate of off-type plant, and grain properties, including 1000 kernel weight, test weight, moisture, ash and protein content, were influenced by year and location. Number of <TEX>$spike/m^2$</TEX>, test weight, moisture, ash and protein content of wheat cultivated in 2011 were higher than those of 2012 and culm length, spike length, rate of off-type plant and 1000 kernel weight of 2012 were higher than those of 2011. Wheat cultivated in southern part of Korea showed higher culm length and 1000 kernel weight and lower test weight than those of northern part of Korea. Spike length, number of <TEX>$spike/m^2$</TEX> and test weight were reduced by additional fertilization after mid of March, although there was no significant difference between date of additional fertilization and grain properties. Cultivated wheats in Jeollabuk-do showed lower ash content and higher protein content than those of other provinces and cultivated wheats in Jeollanam-do exhibited higher ash content than that of other provinces. As amount of fertilization increased, culm length, 1000 kernel weight and protein content increased and spike length and ash content were decreased, although date of additional fertilization did not effect on agronomic characteristics and grain properties. Amount of fertilization was positively correlated with 1000 kernel weight and protein content (r = 0.159, P < 0.05 and r = 0.212, P < 0.01, respectively) and was negatively correlated with ash content (r = -0.185, P < 0.05). Thousand kernel weight was negatively correlated with ash content (r = -0.226, P < 0.01) and positively correlated with protein content (r = 0.207, P < 0.01). Ash content increased as test weight and culm length decreased and 1000 kernel weight was influenced by culm and spike length (r = 0.397, P < 0.001 and r = -0.205, P < 0.01, respectively).

ISSR-derived Molecular Markers for Korean Wheat Cultivar Identification

Jae-Han Son, Kyeong-Hoon Kim, Sanghyun Shin, Hag-Sin Kim, Nam-Soo Kim, Jong-Nae Hyun, Sang-In Shim, Choon-Ki Lee, Kwang-Geun Park, and Chon-Sik Kang. Plant Breeding and Biotechnology 2013;1:262-9. https://doi.org/10.9787/PBB.2013.1.3.262

Two-dimensional Electrophoresis of High Molecular Weight Glutenin Subunits in Korean Wheat Cultivars

Two-dimensional Electrophoresis of High Molecular Weight Glutenin Subunits in Korean Wheat Cultivars

Mineral Compositions of Korean Wheat Cultivars

Twenty-nine Korean wheat cultivars were analyzed for 8 important minerals (Cu, Fe, Mn, Zn, Ca, K, Mg and P) using Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES). A hierarchical cluster analysis (HCA) was applied to classify wheat cultivars, which has a similarity in mineral compositions. The concentration ranges of the micro-minerals Cu, Fe, Mn, and Zn: 0.12~0.71 mg/100 g, 2.89~5.89 mg/100 g, 1.65~4.48 mg/100 g, and 2.58~6.68 mg/100 g, respectively. The content ranges of the macro-minerals Ca, K, Mg and P: 31.3~46.3 mg/100 g, 288.2~383.3 mg/100 g, 113.6~168.6 mg/100 g, and 286.2~416.5 mg/100 g, respectively. The HCA grouped 6 clusters from all wheat samples and a significant variance was observed in the mineral composition of each group. Among the 6 clusters, the second group was high in Fe and Ca, whereas the fourth group had high Cu, Mn and K concentrations; the fifth cluster was high in Zn, Mg and P. The variation in mineral compositions in Korean wheat cultivars can be used in the wheat breeding program to develop a new wheat cultivar with high mineral content, thus to improve the nutritional profile of wheat grains.

Current Status and Future Prospects for Korean wheat Research Using Molecular Markers

Current Status and Future Prospects for Korean wheat Research Using Molecular Markers

Influences of Cultivar and Environment on Arabinoxylan Content in Korean Wheat

Influences of Cultivar and Environment on Arabinoxylan Content in Korean Wheat