Picea Koraiensis Research Articles

Phenotypic Changes in Pinus thunbergii, Larix kaempferi, Picea koraiensis, and Abies holophylla Seedlings Inoculated with Pine Wilt Nematode: Revealing the Resistance

Bursaphelenchus xylophilus (pine wood nematode, PWN) has been present in China for over 40 years and has spread to northeast China, where native pine species are key components of the local top community. Pinus thunbergii is known to be susceptible to PWN among local conifer species, whereas research on PWN’s pathogenicity in Larix remains limited. Furthermore, there are no research reports on PWN infestation in Picea and Abies species within China. This study conducted a detailed analysis of phenotypic changes and temporal spectral reflectance variations in four conifer species in northeast China—P. thunbergii, Larix kaempferi, Picea koraiensis, and Abies holophylla—following artificial inoculation with PWN. The aim of this study is to establish a theoretical basis for identifying the potential hosts and threats of PWN. The study incorporated a 60-day post-inoculation observation period to systematically monitor and compare temporal changes in external morphology, disease susceptibility (incidence and mortality rates), spectral reflectance, and the normalized wilt index (NWI) in 2–3-year-old seedlings of P. thunbergii, L. kaempferi, P. koraiensis, and A. holophylla after inoculation with PWN. The results showed that P. thunbergii displayed the earliest infection symptoms, followed by L. kaempferi, A. holophylla, and finally P. koraiensis. After inoculation, P. thunbergii was the first to experience mortality, followed by L. kaempferi, P. koraiensis, and A. holophylla. Following inoculation, P. thunbergii exhibited the earliest significant increase in NWI (p < 0.001), followed by L. kaempferi and A. holophylla; P. koraiensis showed the latest increase (p < 0.001). In conclusion, the experiment identified P. koraiensis as having the strongest resistance to PWN among the four species, followed by A. holophylla. P. thunbergii showed the weakest resistance, while L. kaempferi exhibited moderate resistance. The ranking of PWN susceptibility for the four conifer species, from highest to lowest, is as follows: P. thunbergii, L. kaempferi, A. holophylla, and P. koraiensis.

Genetic Diversity, Structure, and Differentiation of Picea abies–Picea obovata–Picea koraiensis Species Complex according to Data of Chloroplast DNA Microsatellite Analysis

Genetic Diversity, Structure, and Differentiation of Picea abies–Picea obovata–Picea koraiensis Species Complex according to Data of Chloroplast DNA Microsatellite Analysis

Climatic and edaphic controls of root-tip production and mortality in five temperate tree species

Root tips are the main components of absorptive fine roots, but their seasonal dynamics and relationship to environmental factors remain unclear due to the difficulties in methodology. In this study, we explored the temporal patterns of root-tip production and mortality in monoculture plantations of five temperate tree species at a common site in northeastern China, and identified the general environmental controls on such processes. We made monthly in-situ assessments of root tip length (RTL) production and mortality in two hardwood and three coniferous species with a minirhizotron (MR) method during the growing seasons of 2008 and 2009. Air temperature, rainfall, soil temperature and water content at 10 cm depth were determined concurrently. RTL production in all species exhibited consistent peaks in summer (June–August) in two growing seasons. RTL mortality showed substantial interannual and interspecific variability, with peaks in autumn and winter in 2008, but various patterns in 2009. RTL production positively correlated with monthly soil and air temperature across all species, and with monthly rainfall in three coniferous species. However, there was no significant correlation between RTL production and soil water content. By contrast, RTL mortality was weakly related to environmental factors, showing positive correlations with soil temperature in Korean spruce, and with rainfall in Korean pine and Korean spruce. Our findings suggest that the seasonal patterns of RTL production are convergent across the five temperate tree species due to the overlapped distribution of heat and rainfall, which can conduce roots to maximizing the acquisition of nutrient resources in the soil.

Coniferous Tree Species Identity and Leaf Aging Alter the Composition of Phyllosphere Communities Through Changes in Leaf Traits

Phyllosphere microorganisms are essential for plant growth and health. Although there are an increasing number of studies showing that the composition of phyllosphere communities varies among different plant species, it remains unclear whether and how their bacterial and fungal community composition predictably varies with plant traits and leaf age. In this study, we used high-throughput sequencing to explore the diversity and composition of phyllosphere communities in needles of different ages (originating from different cohorts) for three evergreen coniferous species (Pinus koraiensis, Picea koraiensis, and Abies nephrolepis). Our results indicated that Gammaproteobacteria (bacteria) and Dothideomycetes (fungi) were dominant in newly formed needles, whereas Actinobacteria (bacteria) and Eurotiomycetes (fungi) were dominant in perennial needles. Tree species identity and needle age were the main factors explaining the variations of the α diversity (species richness of phyllosphere communities) and β diversity (dissimilarity among phyllosphere communities). In particular, we found that leaf dry matter content, leaf mass per area, and total phosphorus content emerged as key predictors of composition and diversity of phyllosphere microbial communities, underscoring the major influence of tree species identity and needle age on phyllosphere communities through changes in plant functional traits. Finally, we found that the interaction between tree species identity and needle age also contributed significantly to explaining the diversity and composition of phyllosphere communities, probably because differences in plant functional traits or environmental conditions between new and perennial needles depend on tree growth rates and resource acquisition strategies. These findings provide new insights into the mechanisms of community assembly among different evergreen tree species and offer a better understanding of the interactions between plant traits and phyllosphere microorganisms during needle aging.

Changes in Spatiotemporal Pattern and Its Driving Factors of Suburban Forest Defoliating Pest Disasters

Forest defoliating pests are significant global forest disturbance agents, posing substantial threats to forest ecosystems. However, previous studies have lacked systematic analyses of the continuous spatiotemporal distribution characteristics over a complete 3–5 year disaster cycle based on remote sensing data. This study focuses on the Dendrolimus superans outbreak in the Changbai Mountain region of northeastern China. Utilizing leaf area index (LAI) data derived from Sentinel-2A satellite images, we analyze the extent and dynamic changes of forest defoliation. We comprehensively examine the spatiotemporal patterns of forest defoliating pest disasters and their development trends across different forest types. Using the geographical detector method, we quantify the main influencing factors and their interactions, revealing the differential impacts of various factors during different growth stages of the pests. The results show that in the early stage of the Dendrolimus superans outbreak, the affected area is extensive but with mild severity, with newly affected areas being 23 times larger than during non-outbreak periods. In the pre-hibernation stage, the affected areas are smaller but more severe, with a cumulative area reaching up to 8213 hectares. The spatial diffusion characteristics of the outbreak follow a sequential pattern across forest types: Larix olgensis, Pinus sylvestris var. mongolica, Picea koraiensis, and Pinus koraiensis. The most significant influencing factor during the pest development phase was the relative humidity of the year preceding the outbreak, with a q-value of 0.27. During the mitigation phase, summer precipitation was the most influential factor, with a q-value of 0.12. The combined effect of humidity and the low temperatures of 2020 had the most significant impact on both the development and mitigation stages of the outbreak. This study’s methodology achieves a high-precision quantitative inversion of long-term disaster spatial characteristics, providing new perspectives and tools for real-time monitoring and differentiated control of forest pest infestations.

Analysis of PM2.5 Concentration Released from Forest Combustion in Liangshui National Natural Reserve, China

(1) Background: In recent years, forest fires have become increasingly frequent both domestically and internationally. The pollutants emitted from the burning of fuel have exerted considerable environmental stress. To investigate the influence of forest fires on the atmospheric environment, it is crucial to analyze the variations in PM2.5 emissions from various forest fuels under differing fire conditions. This assessment is essential for evaluating the effects on both the atmospheric environment and human health. (2) Methods: Indoor simulated combustion experiments were conducted on the branches, leaves, and bark of typical tree species in the Liangshui National Natural Reserve, including Pinus koraiensis (PK), Larix gmelinii (LG), Picea koraiensis (PAK), Betula platyphylla (BP), Fraxinus mandshurica (FM), and Populus davidiana (PD). The PM2.5 concentrations emitted by six tree species under various combustion states were measured and analyzed, reflecting the impact of moisture content on the emission of pollutants from fuel combustion, as indicated by the emission factors for pollutants. (3) Results: Under different fuel loading and moisture content conditions, the mass concentration values of PM2.5 emitted from the combustion of different organs of various tree species exhibit variability. (4) Conclusions: Among the various tree species, broad-leaved varieties release a greater quantity of PM2.5 compared to coniferous ones. A positive correlation exists between the moisture content of the fuel and the concentration of PM2.5; changes in moisture content notably influence PM2.5 levels. The emission of PM2.5 from fuel with varying loads increases exponentially. Utilizing the Response Surface Methodology (RSM) model for simulation, it was determined that both moisture content and fuel load exert a significant combined effect on the release of PM2.5 during combustion.

Prolonged effect of growth stimulants on cultivation of planting material of Koyama spruce Picea koraiensis Nakai

Koyama spruce (Picea koraiensis Nakai) is one of the main tree species in Primorsky Krai. Due to its rapid growth, light loving characteristics and good renewal on wood cutting and burned areas in combination with high technical qualities and decorative properties that distinguish it from other Far Eastern spruces, as well as positive experience of plants in various regions of Russia, this tree species is one of the promising coniferous species of the Russian Far East for forest restoration. Koyama spruce is valuable for landscaping cities and towns. However, its introduction is constrained by a number of factors, including a lack of quantity and a high cost of seed material. Use of growth stimulants accelerates production of high-quality planting material of spruce. The purpose of the research was to study the prolonged effect of pre-planting treatment of the root system of three-year-old saplings of Koyama spruce with growth stimulants Krezatsin (triethanolammonium salt of orthocresoxyacetic acid 475 g/l), Tsirkon (hydroxycinnamon acids) and Epin-Extra (epibrassinolide solution in alcohol 0.025 g/l) on morphometric parameters of one- and four-year-old seedlings. Three-year-old seedlings of Koyama spruce grown in the seedling section of the nursery were transplanted to nursery-garden on the area of 0.2 hectares. The seedlings were planted at a distance of 0.61 m. Before transplanting, their root system was placed for 0.5 hours in solutions of growth stimulants Krezatsin, Tsirkon and Epin-Extra (solution concentrations of 1 ml / 5 l and 1 ml / 10 l of water). Control saplings were not treated. At the end of the growing season at the end of the 1st and 4th years of growth, the saplings were measured in terms of stem height, height increase, and diameter of root collar. It was revealed that Krezatsin and Epin-Extra growth stimulants had a significant effect on both the growth of one- and four-year-old seedlings So, in the first year of growth, the height of seedlings exceeded the control by 6.316.2 %, the increase in height - by 27.555.5 %, the diameter of root collar - by 4080 %; in the fourth year, the excess of seedlings to control was 29.945.4, respectively; 7.651.5 and 33.355.6 %. It was found that the concentration of solutions of 1 ml / 10 l was more effective.

Phytochemical Analysis, Antioxidant and Enzyme-Inhibitory Activities, and Multivariate Analysis of Insect Gall Extracts of Picea koraiensis Nakai.

Picea koraiensis Nakai (PK) is an evergreen tree. It plays an important role in landscaping and road greening. Insect galls of PK are formed by parasitism of the adelgid Adelges laricis. Except for phenolics, other chemical constituents and biological activity of insect gall from PK are still unknown. Thus, here, we performed phytochemical and biological activity analyses of PK insect gall extracts, aiming to turn waste into treasure and serve human health. PK insect gall extracts were prepared using seven solvents. Antioxidant activities of the extracts were examined via antioxidant assays (radical and oxidizing substance quenching, metal chelating, and reducing power). The inhibitory activities of the extracts were determined toward the key human-disease-related enzymes α-glucosidase, α-amylase, cholinesterase, tyrosinase, urease, and xanthine oxidase. The content of numerous active constituents was high in the methanol and ethanol extracts of PK insect gall, and these extracts had the highest antioxidant and enzyme-inhibitory activities. They also showed excellent stability and low toxicity. These extracts have potential for use as stabilizers of olive and sunflower seed oils.

Nonlinear model prediction of needle chlorophyll content of Picea koraiensis Nakai at different needle ages based on hyperspectral features

Pigment content is a critical assessment indicator in the study of plant physiological metabolism, stress resistance, ornamental characteristics, and forest health. Spectral imaging technology is widely used for rapid and non-destructive determination of plant physicochemical parameters. To address the shortcomings of previous models of spectral reflectance prediction of chlorophyll content of needles only from the perspective of traditional algorithms and ignoring physical models, this research integrates variable complexity and refined classification of physical models to validate the increased accuracy of both the conventional partial least squares (PLS) method and the traditional neural network algorithm. The results of the conifer chlorophyll models of Picea koraiensis Nakai with different needle ages based on spectral reflectance and vegetation index parameters showed that the improved nonlinear state transition algorithm-backpropagation (STA-BP) neural network model approach (R2 of 0.73–0.89) and the nonlinear Stacking partial least squares (Stacking-PLS) model approach (R2 of 0. 67–0.85) is slightly more robust than the traditional algorithms nonlinear BP model (R2 of 0.63–0.82) and linear PLS model (R2 of 0.60–0.76). This finding suggests that the nonlinear fitting of chlorophyll content in needles of different needle ages in P. koraiensis Nakai surpasses the traditional linear model fitting methodology. Furthermore, the model fitting of chlorophyll content in conifers of different needle ages outperforms the mixed P. koraiensis Nakai model, suggesting that chlorophyll models using needle refinement classification help to improve model robustness. This study provides data and theoretical support for rapid and non-invasive characterization of physiological and biochemical properties of needles of different needle ages using spectral imaging techniques to predict growth and community structure productivity of forest trees in the coming years.

Carbon Accounting of Weihe CSA Pilot Demonstration Area in Longjiang Forest Industry

Carbon sink afforestation (CSA) has become one of the most concerned issues of countries around the world under the background of climate change. The northern forest ecosystem, located in mid- and high latitudes, is a huge terrestrial carbon pool and is very sensitive to climate change. Studying the carbon emission accounting of CSA in northern forests helps clarify the contribution of CSA to forestry carbon sequestration and forecasts the carbon sink effect of forest ecosystems. It is of great significance for the assessment of forest carbon sink and carbon cycling by providing a scientific basis and reference for the development, utilization, and management of carbon sink afforestation, as well as the coordinated development of ecology and social economy. At present, research on the carbon emission accounting of the CSA in northern China is still lacking. According to the relevant models and parameters of estimating live biomasses with the default method from the IPCC’s (Intergovernmental Panel on Climate Change) Technical Guidelines for National Forestry Carbon Sink Accounting and Monitoring, carbon stock, carbon emission, and carbon leakage of the Weihe CSA (carbon sink afforestation) pilot demonstration area in the boreal Longjiang Forest Industry in a baseline scenario and CSA scenario were measured, and the CSA’s net carbon sink was estimated. The conclusions were as follows: (1) By the end of the crediting period of the project’s baseline, carbon fixation reached 101.85 t CO2, with an average annual CO2 fixation of 5.09 t. By the end of the CSA term, carbon sequestration was accumulated as 382.13 t CO2, with an average annual sequestration of 19.11 t CO2, nearly four times that of the baseline period. (2) By the end of the CSA term, the carbon sequestration of the coniferous standing forest was 46.2% higher than that of the broad-leaved standing forest, accounting for 65% of the total carbon sequestration of the forest. The carbon sequestration of the tree species in the coniferous forest in descending order is Picea koraiensis, Pinus koraiensis, Larix olgensis, Fraxinus mandshurica, and Populus cathayana. The carbon sink density of the coniferous standing forest was 8.7% higher than that of the broad-leaved standing forest. (3) The carbon fixation of the coniferous standing forest nearly doubled that of the broad-leaved standing forest. The highest carbon fixation belongs to Fraxinus mandshurica, closely followed by Picea koraiensis and Pinus koraiensis at a high level, and then Larix olgensis and Populus cathayana. The carbon fixation of Fraxinus mandshurica was 20 times that of Populus cathayana. (4) The accumulated greenhouse gas emissions within the boundary during the CSA period were 2.53 t CO2-e. The accumulated greenhouse gas leakage outside the boundary was 0.05 t CO2-e. Carbon emissions occurred in the first, second, and third years of the crediting period, while carbon leakage occurred only in the first year. (5) This result appeared as carbon sources during the first three years of the CSA period but changed to carbon sink from the fourth year and then accumulated to 280.28 t (70.07 t CO2-e·hm−2) as a net carbon sink by the end of the term. The Weihe CSA appeared to have a relatively strong ability of carbon sequestration in temperate forests. The CSA activity is influenced by factors such as policies, environment, management, etc., resulting in uncertainties in carbon sequestration accounting. Therefore, it is suggested that comparison studies and investigations should be strengthened, and multiple methods should be integrated into carbon sequestration estimation and accounting, leading the carbon accounting of forest ecosystems to a high-level and comprehensive development.

Shift of root nitrogen-acquisition strategy with tree age is mediated by root functional traits along the collaboration gradient of the root economics space.

Root nitrogen (N)-uptake rate and uptake preference, and their association with root morphological and chemical traits are important to characterize root N-acquisition strategies of trees. However, how the root N-acquisition strategy varies with tree age, especially for those species that coexist at a common site, remains unknown. In this study, a field isotopic hydroponic method was used to determine the uptake rate and contribution of NH4+, NO3- and glycine, for three coexisting ectomycorrhizal coniferous species [Pinus koraiensis (Korean pine), Picea koraiensis (Korean spruce) and Abies nephrolepis (smelly fir)] at three age classes (young, middle-aged and mature) in a temperate forest. Concurrently, root morphological and chemical traits, as well as mycorrhizal colonization rate were determined. Our results show that the root uptake rate of total N and NH4+ gradually decreased across all three species with increasing tree age. The three species at all age classes preferred NH4+, except for middle-aged Korean spruce and mature smelly fir, which preferred glycine. In contrast, all three species showed the lowest acquisition of NO3-. According to the conceptual framework of 'root economics space', only a 'collaboration' gradient (i.e. dimension of root diameter vs specific root length or area) was identified for each species, in which root N-uptake rate loaded heavily on the side of 'do-it-yourself' (i.e. foraging N more by roots). Young trees of all species tended to exhibit the 'do-it-yourself' strategy for N uptake, and mature trees had an 'outsourcing' strategy (i.e. foraging N by a mycorrhizal partner), whereas middle-aged trees showed a balanced strategy. These findings suggest that shifts of root N-acquisition strategy with tree age in these species are mainly mediated by root traits along the 'collaboration' gradient, which advances our understanding of belowground competition, species coexistence and N cycling in temperate forests.

Leaf-branch vulnerability segmentation occurs all year round for three temperate evergreen tree species

Vulnerability segmentation (VS) and Hydraulic segmentation (HS) hypotheses propose higher hydraulic resistance and vulnerability to embolism in leaves than in branches, respectively. The VS and HS are suggested as an acclimation strategy of trees to drought stress, but whether they occur during freezing stress has rarely been explored. We measured the leaf and branch hydraulic traits of three temperate evergreen tree species [Picea koraiensis (Korean spruce), Pinus koraiensis (Korean pine), and Pinus sylvestris var. mongolica (Mongolian pine)] during four seasons (winter, spring, summer, and autumn) across the year. We assessed the applicability of VS and HS all year round, particularly in winter. The water potential at which leaf hydraulic conductance lost 50% (P50L), was more negative in winter than in summer, while higher leaf mass per area was obtained in winter. These results suggest that these species invest more carbon into leaf (including hydraulic systems) to acclimate to winter frost drought. Leaf and branch hydraulic conductance (KmL and KmB) were lower, and the percentage loss of branch hydraulic conductance (PLCB) was higher in spring than in autumn. These results were probably because of more freeze-thaw cycles in spring (69 cycles) than in autumn (37 cycles). The water potential at which branch hydraulic conductance lost 50%, P50B, was more negative than P50L across the year. The values of VS (P50L minus P50B) were positive, i.e. leaf was more vulnerable than the branch in all species and across seasons, with higher values occurring in spring or autumn. However, KmL positively correlated with KmB, suggesting hydraulic coordination between leaf and branch, but did not support HS. Our findings indicate that leaf-branch vulnerability segmentation can occur all year round, including freezing stress, to protect branches from hydraulic failure in temperate evergreen conifers.

Population Structure, Genetic Diversity and Candidate Genes for the Adaptation to Environmental Stress in Picea koraiensis.

Picea koraiensis is major silvicultural and timber species in northeast China, and its distribution area is an important transition zone for genus spruce migration. The degree of intraspecific differentiation of P. koraiensis is high, but population structure and differentiation mechanisms are not clear. In this study, 523,761 single nucleotide polymorphisms (SNPs) were identified in 113 individuals from 9 populations of P. koraiensis by genotyping-by-sequencing (GBS). Population genomic analysis showed that P. koraiensis was divided into three geoclimatic regions: Great Khingan Mountains climatic region, Lesser Khingan Mountains climatic region, and Changbai Mountain climatic region. Mengkeshan (MKS) population on the northern edge of the distribution area and Wuyiling (WYL) population located in the mining area are two highly differentiated groups. Selective sweep analysis showed that MKS and WYL populations had 645 and 1126 selected genes, respectively. Genes selected in the MKS population were associated with flowering and photomorphogenesis, cellular response to water deficit, and glycerophospholipid metabolism; genes selected in the WYL population were associated with metal ion transport, biosynthesis of macromolecules, and DNA repair. Climatic factors and heavy metal stress drives divergence in MKS and WYL populations, respectively. Our findings provide insights into adaptive divergence mechanisms in Picea and will contribute to molecular breeding studies.

Evaluating the Legacy Effects of the Historical Predatory Seed Harvesting on the Species Composition and Structure of the Mixed Korean Pine and Broadleaf Forest from a Landscape Perspective

Adequate seed provenance is an important guarantee for the restoration of the mixed Korean pine (Pinus koraiensis) and broadleaf forest (MKPBF). However, the commercial harvest exclusion in natural forests has led to a sharp decline in economic income from timber. Given the economic value of Korean pine seeds, predatory seed harvesting (PSH) has become increasingly serious in the past 20 years and has significantly reduced the seed and seedling bank, which might seriously threaten the restoration and sustainable management of the MKPBF. How the historical PSH has affected the future of the MKPBF is unclear at the landscape scale. In this study, we quantified the effects of seed harvesting at the landscape scale by a forest landscape model LANDIS PRO, and then assessed the legacy effects of the historical PSH on the composition and structure of the MKPBF in the Xiaoxing’an Mountains, Northeast China. Our results showed that the historical PSH decreased the Korean pine basal area of all age cohorts, with an average decrease of 0.06 to 0.19 m2 ha−1 but insignificantly altered the age structure diversity of Korean pine throughout the simulation. Our results indicated that the historical PSH remarkably decreased the dominance of Korean pine by 11.1%, but significantly increased the dominance of spruce (Picea koraiensis and Picea jezoensis) and fir (Abies nephrolepis) by 3.8% and 4.5%, respectively, and had an insignificant effect on the other tree species over the simulation. We found that the historical PSH evidently changed the succession trajectories of the disturbed stands, which would result in the transition from the succession pattern dominated by Korean pine to that dominated by spruce and fir. The historical PSH decreased the importance value of Korean pine by 12.2% on average but increased it by 5.1% and 6.0% for spruce and fir, respectively, and resulted in an average 33.2% increase in the dissimilarity index compared with the initial state during the whole simulation period. Future forest management should strictly limit the intensity and rotation of seed harvesting to protect the seed provenance of Korean pine and consider how to ensure the recovery and sustainable management of the MKPBF through direct seeding or seedling planting.

Effects of thinning on soil nutrient availability and fungal community composition in a plantation medium-aged pure forest of Picea koraiensis

Thinning is an important silvicultural practice for improving the productivity and wood production in plantation forest. Different intensities of thinning management can affect tree growth and alter soil nutrient effectiveness, thus affecting soil fungal community structure and diversity. Our objective is to determine the soil factors and their regulatory mechanisms that influence stand growth by thinning, and to provide data to support the establishment of large diameter timber cultivation technology for Picea koraiensis. In this study, we conducted medium- and high-intensity thinning in 43a P. koraiensis plantation middle-aged forests and investigated the growth indexes, soil physicochemical properties, and fungal community diversity in rhizosphere and non-rhizosphere soils of the stands after thinning at different densities (904 plants/ha for control, 644 plants/ha for 30% thinning intensity, and 477 plants/ha for 50% thinning intensity). The results showed that all growth indicators (annual growth of tree height, diameter at breast height, height under live branches and crown width) of the plantation after high-intensity thinning (477 plants/ha) were higher than those of the control (no thinning, significant) and medium-intensity thinning (644 plants/ha). Mycorrhizal infection rate was higher at the beginning of the growing season than at the end of the growing season, and increased slightly with decreasing stand density. Compared to the control, all medium- and high-intensity thinning treatments significantly improved soil nutrient content (P < 0.05), including total carbon, total nitrogen, total phosphorus, total potassium, Available phosphorus and Available potassium. Fungal diversity was higher but lower in abundance than the control in both rhizosphere and non-rhizosphere soils after thinning. The number of OTUs and fungal richness and diversity indices of non-rhizosphere soil fungi were higher than those of rhizosphere soil fungi. In conclusion, this study provides new evidence that reasonable intercalation can increase the radial and vertical growth of P. koraiensis plantation forests and promote the diversity of subsurface soil fungal communities. It is shown that thinning intensity regulates biogeochemical cycles in P. koraiensis plantation ecosystems by affecting soil nutrients and fungal community structure. Therefore, 50% thinning intensity can be used to increase timber production in plantation forests during large diameter timber cultivation of P. koraiensis and improve predictions associated with achieving long-term forest management strategies.

Historical temperature variability in a representative high-latitude region in the monsoon-continental climate transition zone in China

Global warming has exacerbated the instability of the climate and has led to the frequent occurrence of extreme cold and warm events. The complex geography of the high-latitude region of China's monsoon-continental transition zone makes it extremely sensitive to climate response, understanding temperature changes over long periods of time is crucial to revealing trends in climate change trends. In this study, we constructed a standardized tree-ring width chronology of Korean spruce growing in this area from 1845 to 2016 and used it to analyzed the response of the radial growth of Korean spruce to climatic factors such as temperature and precipitation. The results show that the annual mean temperature was the dominant climatic factor affecting the growth of Korean spruce. Hence, we reconstructed the annual mean temperature series of this region spanning the past 172 years. The analysis results show that the study area experienced five warm periods and five cold periods in the past 172 years. Cold years were dominant before 1960s, while temperature continuously rose and changed drastically in the early twenty-first century. The reconstructed annual mean temperature series has variability cycles of 3a, 7a, 10–12a, 15–22a and 30–40a. The results of this reconstruction enrich the tree-ring database in the representative regions of the monsoon-continental climate transition zone in China and provide a reference for systematically understanding the climate change patterns in the representative regions of the transition zone.

Mixed conifer-broadleaf trees on arbuscular mycorrhizal and ectomycorrhizal communities in rhizosphere soil of different plantation stands in the temperate zone, Northeast China.

In comparison with ectomycorrhizal (EM) tree species, arbuscular mycorrhizal (AM) trees have different litter quality and nitrogen cycle modes, which may affect mycorrhizal colonization and the community composition and diversity. However, available studies addressing the mycorrhizal fungal colonization rate, diversity and community composition in mixed forest stands composed of AM and EM trees are rare. In the present study, we assessed litter quality, soil physicochemical properties and correlated them with mycorrhizal community characteristics in rhizosphere soils of monoculture and mixture plantation stands of AM tree species (Fraxinus mandschurica Rupr.) and EM tree species (Larix gmelinii Rupr., Picea koraiensis Nakai) in Northeast China. We hypothesized that (1) the effect of mixture pattern on mycorrhizal colonization rate and diversity would change with tree species, (2) the effect of mixture pattern on mycorrhizal community composition would be less pronounced in comparison with that of tree species. We found that mixture did not change AMF colonization rate regardless of mixture identity, whereas mixture and tree species exerted significant effects on EMF colonization rate. For AMF community, both M-AS (Fraxinus mandschurica Rupr. and Picea koraiensis Nakai) and M-AL (Fraxinus mandschurica Rupr. and Larix gmelinii Rupr.) mixtures significantly increased Pielou index and Simpson index, whereas only M-AS significantly increased Sobs. For EMF community, mixture significantly affected examined diversity indices except for Chao1. Mixture significantly shifted AMF and EMF community, and the magnitude was tree species dependent. The dominant genera in AMF and EMF communities in plantation stands were Glomus and Tomentella, respectively. The EnvFit analysis showed that the determinant factors of EMF community are soil moisture, pH, nitrate nitrogen content, dissolved organic nitrogen content, soil organic matter content, soil organic carbon/total nitrogen and litter carbon/total nitrogen. In conclusion, mixed conifer-broadleaf trees significantly changed soil physicochemical properties, litter quality as well as mycorrhizal fungi community diversity and composition.

Pathophysiology and transcriptomic analysis of Picea koraiensis inoculated by bark beetle-vectored fungus Ophiostoma bicolor.

Ophiostomatoid fungi exhibit a complex relationship with bark beetles; exhausting of host tree defenses is traditionally regarded as one of the key benefits provided to beetle vectors. Ophiostoma bicolor is one of the dominant species of the mycobiota associated with Ips genus bark beetles which infect the spruce trees across the Eurasian continent. Host spruce trees resist fungal invasion through structural and inducible defenses, but the underlying mechanisms at the molecular level, particularly with respect to the interaction between bark beetle-associated fungi and host trees, remain unclear. The aim of this study was to observe the pathological physiology and molecular changes in Picea koraiensis seedlings after artificial inoculation with O. bicolor strains (TS, BH, QH, MX, and LWQ). This study showed that O. bicolor was a weakly virulent pathogen of spruce, and that the virulent of the five O. bicolor strains showed differentiation. All O. bicolor strains could induce monoterpenoid release. A positive correlation between fungal virulence and release of monoterpenoids was observed. Furthermore, the release rate of monoterpenoids peaked at 4 days post-inoculation (dpi) and then decreased from 4 to 90 dpi. Transcriptomic analysis at 4 dpi showed that many plant-pathogen interaction processes and mitogen-activated protein kinase (MAPK) metabolic processes were activated. The expression of monoterpenoid precursor synthesis genes and diterpenoid synthesis genes was upregulated, indicating that gene expression regulated the release rate of monoterpenoids at 4 dpi. The enriched pathways may reveal the immune response mechanism of spruce to ophiostomatoid fungi. The dominant O. bicolor possibly induces the host defense rather than defense depletion, which is likely the pattern conducted by the pioneers of beetle-associated mycobiota, such as Endoconidiophora spp.. Overall, these results facilitate a better understanding of the interaction mechanism between the dominant association of beetles and the host at the molecular level.

Antimicrobial diterpene induced by two gall-inducing adelgids coexisting on Picea koraiensis.

The mechanism by which closely related species can coexist is a central factor in the stability of ecological communities. The larch adelgid (Adelges laricis laricis) and the eastern spruce adelgid (Adelges (Sacchiphantes) abietis) have both been found on the branches of Picea koraiensis in China. These two adelgids exhibit strong infectivity and readily induce the formation of 'fish scale-like' and 'pineapple-like' galls with branch parasitism rates of between 75.01±7.03 and 88.02±4.39%. Interestingly, the gall tissues in which these two gall-inducing insects were found to be coexisting were discovered at a rate of ~0.2% in the studied populations. The weight and number of gall chambers as well as the number of adelgids in the 'fish scale-like' side were higher than those in the 'pineapple-like' side. Furthermore, compared with the normal branches, a diterpene neoabietic acid was found at elevated concentrations in the gall tissues, with especially high concentrations seen in the tissues of the co-occupied galls. Neoabietic acid exhibited strong antibacterial activities against Bacillus spp. isolated from the branches of P. koraiensis, as well as potent antifungal activity against the hyphal growth of Fusarium graminearum JMY-1, which was obtained from the gall tissues. Our result provides evidence that the coexistence of the two closely related species could be explained by alterations of the host tissues by the insects resulting in increased concentrations of the antimicrobial agent.

Hydraulic Function Analysis of Conifer Xylem Based on a Model Incorporating Tracheids, Bordered Pits, and Cross-Field Pits

Wood has a highly complex and anisotropic structure. Its xylem characteristics are key in determining the hydraulic properties of plants to transport water efficiently and safely, as well as the permeability in the process of wood impregnation modification. Previous studies on the relationship between the xylem structure and hydraulic conductivity of conifer have mainly focused on tracheids and bordered pits, with only a few focusing on the conduction model of cross-field pits which connect tracheids and rays. This study takes the xylem structure of conifer as an example, drawing an analogy between water flow under tension and electric current, and extends the model to the tissue scale, including cross-field pits by establishing isometric scaling. The structure parameters were collected by scanning electron microscopy and transmission electron microscopy. The improved model can quantify the important hydraulic functional characteristics of xylem only by measuring the more easily obtained tracheid section size. Then, this model was applied to quantify the relationship between the xylem anatomical structure and hydraulic properties in the pine (Pinus sylvestris L. var. mongholica Litv.) and the spruce (Picea koraiensis Nakai), and also to evaluate the effects of the number and size of cross-field pits on xylem conduction. The results showed that the growth ring conduction value of the pine was more than twice that of the spruce for the two tree species with similar growth widths in this study. The tracheid wall resistance of the pine reflected the result of the interaction of the size and number of cross-field pits, in comparison, the wall resistance of the spruce was more sensitive to the number of cross-field pits. Finally, the calculation output of the new model was cross-validated with the literature, which verified the accuracy and effectiveness of the model. This study provides an effective and complete solution for xylem conductivity measurement and the study of wood ecophysiological diversity and processing.