Korean Black Goats Research Articles

동계사료작물 사일리지를 첨가한 TMR 급여가 흑염소의 사료섭취량, 영양소 소화율 및 혈액성상에 미치는 영향

본 연구는 동계사료작물 사일리지를 첨가한 TMR을 급여하였을 때, 흑염소의 사료 섭취량, 일당 증체량, 영양소 소화율 및 질소 축적율에 미치는 영향을 조사하여 흑염소에 대한 동계사료작물의 사료가치 평가의 자료로 이용하고자 실시하였다. 공시가축은 흑염소 12두(♂)를 4처리구(T1: 청보리 사일리지 첨가구, T2: 유채사일리지 첨가구, T3: 호밀 사일리지 첨가구 및 T4: 이탈리안 라이그라스 사일리지 첨가구)로 나누어 처리구당 3두씩 개체별 대사케이지 에 라틴방각법으로 실시하였으며 그 결과는 다음과 같다. 1일 두당 건물, 유기물 및 조단백질 섭취량은 유채 사일리지 첨가구가 가장 높았고, 청보리 사일리지 첨가구가 가장 낮았다(p<0.05). 조단백질 및 ADF 가소화 섭취량 또한 유채 사일리지 첨가구가 가장 높았다(p<0.05). 1일 증체량은 유채 사일리지 첨가구, 호밀 사일리지 첨가구 및 이탈리안 라이그라스 사일리지 첨가구가 청보리 사일리지 첨가구 보다 유의하게 높았다(p<0.05). 건물, 조단백질 및 섬유소 소화율은 청보리 사일리지 첨가구가 가장 높았으며, 유채 사일리지 첨가구가 유의하게 낮았다(p<0.05). 질소섭취량과 질소축적율은 유채 사일리지 첨가구가 유의하게 높았으며, 청보리 사일리지 첨가구가 가장 낮았다(p<0.05). 이러한 결과를 종합적으로 고려할 때 동계사료작물 중 유채 사일리지 첨가시 사료 섭취량 및 질소 축적율 증가로 인해 흑염소의 생산성이 높은 것으로 나타났다. This study was conducted to determine the feed value of total mixed rations (TMR) containing different winter forage crop silages on feed intakes, daily gain, nutrient digestibility, and nitrogen retention in Korean black goats. A total of 12 male goats were used in this study and each goat was housed in individual metabolism crates. The treatments were four diets, consisting of whole crop barley silage (T1), rape silage (T2), rye silage (T3), and Italian ryegrass silage (T4). The experiment was designed as a <TEX>$4{\times}4$</TEX> Latin square arrangement in three replicates. Daily intakes for dietary dry matter (DM), organic matter (OM), crude protein (CP) per head were highest in rape silage treatment, and lowest in whole barley silage (p<0.05). Digestible intakes for CP and acid detergent fiber were highest in rape silage treatment. The daily gain of rape, rye, and Italian ryegrass silages were significantly (p<0.05) higher than that of whole crop barley silage. Digestibility of DM. CP, fiber were highest in whole crop barley silage, and lowest in rape silage (p<0.05). Nitrogen (N) intake and N retention were significantly highest in rape silage and lowest in whole crop barley silage (p<0.05). The results showed that, among different winter forage crop silages, rape silage incorporation into TMR had the best performances indicating increases of feed intakes and N retention in Korean black goats.

흑염소를 이용한 유기초지조성과 식생 개선 효과

본 연구는 제경법을 이용한 유기초지 조성기술을 확립하기 위하여 2005년부터 2007년까지 3년 동안 수행하였으며 방목 축으로는 흑염소가 사용되었다. 시험설계는 관행조성구인 대조구, 퇴구비시용 후 방목구, 방목 후 퇴구비 시용구, 방목 후 퇴구비 시용 재방목구로 처리하였다. 목초정착률은 관행구인 대조구가 유기조성구에 비하여 현저하게 증가하였으며(p<0.05), 또한 건물생산성도 유기조성구에 비하여 대조구에서 현저한 증가를 나타냈다. 조성 후 연차가 경과함에 따라 식생 및 건물생산성이 개선되어 조성효과와 아울러 조성 후 관리의 중요성을 인식할 수 있다. 목초의 사료가치는 대조구와 유기조성구 모두 큰 차이는 나타나지 않았으며, 화본과 목초의 비율은 조성1년차가 2년차에 비하여 높은 경향을 보였다. 시험 종료 후 토양 성분을 조사한 결과 pH와 양이온치환용량은 시험전과 거의 변화가 없었으나 유기물과 인산 함량은 약간 증가하였다. This study was carried out to determine the effect of establishment of organic pasture by hoof cultivation with Korean black goat on productivity of grassland and properties of soil. This study was conducted from August, 2005 to September, 2008 at Cheonan, Korea. Experiment was designed composing with 4 treatment : Standard (Control, T1), Grazing after application of cattle manure (T2), Application of cattle manure after grazing (T3), Application of cattle manure at half of grazing (T4). The establishment ratio of pasture in control treatment increased significantly as compared with that of organic treatment(p<0.05). The yield of dry matter (DM) of pasture in control treatment increased significantly as compared with that of organic treatment. The contents of crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF) and dry matter digestibility (DMD) of pasture were not influenced by cattle manure and grazing method. Grasses coverage ratio in grassland in second year after grassland development were higher than that of first year. The contents of organic matter (OM) and <TEX>$P_2O_5$</TEX> in soil samples collected in organic treatment at the end of the experiment were higher than those of control. However, The pH, and the concentrations of CEC (Ca, Na and Mg) in soil samples collected in organic treatment were hardly influenced, as compared with those at the beginning of the experiment. This study suggests that the grasses and legumes coverage ratio in grassland can be improved by suitable pasture management.

Effects of Crude Protein Levels in Total Mixed Rations on Growth Performance and Meat Quality in Growing Korean Black Goats

This study was carried out to investigate effects of crude protein (CP) levels in total mixed rations (TMR) on growth performance and meat quality in growing Korean black goats. Thirty-six 6-month-old growing black male goats (initial body weight = 17.95±0.13 kg) were used. The goats were randomly divided into four treatments and 9 animals were allotted to each treatment. In terms of treatments, CP levels in TMR were 14, 16, 18, and 20% of dry matter (DM) and all diets were isocaloric (2.4 Mcal ME/kg DM). The experiment lasted for 20 weeks with a 15-day adaptation period. After the end of the trial, five goats from each treatment were slaughtered to measure carcass characteristics and meat quality. Average daily gain (ADG) significantly (p<0.05) increased as dietary CP levels increased, but the highest ADG was found on the 18% CP treatment. The higher CP levels in TMR significantly (p<0.05) decreased feed conversion ratio, but the lowest ratio was on the 18% CP treatment, suggesting a higher bioavailability of nutrients by balanced energy and protein levels on this treatment. There was no significant difference in dressing percentage among treatments, but there was a tendency for levels more than 16% CP in TMR to have a comparatively higher dressing percentage than the 14% treatment. The percentages of meat, fat and bone were not significantly influenced by dietary CP levels. Dietary CP levels did not influence chemical composition of goat meat. Furthermore, there was no significant difference among treatments in any of the sensory parameters except for tenderness. Tenderness was similar for 18 and 20% treatments, which were significantly (p<0.05) higher than the 14% treatment. The results from this study suggested that an adequate CP level in TMR for achieving optimal growth performance and meat quality of growing Korean black goats might be 18% of DM, and that dietary CP level above 18% seemed not to further increase growth performance and meat quality.

사료급여체계가 재래종 및 교잡종 흑염소의 영양소 이용율, 질소축적 및 혈액성상에 미치는 영향

본 시험은 흑염소의 효율적인 사양관리체계의 확립에 기여하기 위하여, 재래흑염소 10두(male)와 교잡흑염소(재래흑염소<TEX>$\times$</TEX>호주야생염소 교잡종; 50:50%) 10두(male)를 공시하여, 시판 배합사료와 조사료를 급여하는 관행사료와 TMR 사료를 각각 급여하였을 때, 흑염소의 사료섭취량, 영양소소화율, 질소축적율 및 혈액성상에 미치는 영향을 조사하였으며, 실험기간은 사료 적응기간 14일 예비기간 10일을 거친 후, 14일간의 본 실험 기간을 두어 총 38일간 실시하였다. 결과를 살펴보면 1일 두당 건물의 섭취량은 TMR을 급여한 교잡종이 가장 높았고(p<0.05), 품종간에서는 재래종 보다 교잡종지, 사료급여체계간에는 관행구보다 TMR구가 높았다(p<0.05). 일당증체량은 재래종 관행구, 재래종 TMR구, 교잡종 관행구, 교잡종 TMR구의 순으로 유의하게 높아졌다(p<0.05). 건물 소화율은 사료급여체계간에서는 TMR구가 관행구보다 유의하게 높았으며, 품종간에도 교잡종이 재래종 보다 높았다(p<0.05). 질소축적량 및 질소축적율은 교잡종 TMR구가 가장 높았고, 재래종 관행구가 가장 낮았다(p<0.05). 혈중 glucose의 함량과 PUN(plasma urea nitrogen)은 교잡종이 재래종 보다 유의하게 높았다(p<0.05). 이상의 결과를 종합하면, TMR 형태의 사료급여가 재래종 보다는 교잡종에 흑염소의 생산성과 사료섭취량, 소화율, 질소축적을 및 혈액성상에 양호한 영향을 나타내어, 효율적인 사양관리체계의 확립과 함께 흑염소 생산농가에 경제적 이득을 줄 수 있을 것으로 판단된다. This trial was conducted to investigate effects of different feeding types of conventional or total mixed rations (TMR) provision on feed intake, nutrient digestibility, nitrogen retention and blood metabolite characteristics in different breeds of native and cross bred Korean black goats. Ten Korean native male goats and 10 Korean crossbred black male goats (Korean native <TEX>$\times$</TEX> Australian feral crossbred, 50:50), average 18 kg of BW, were used in this trial, and each breed of Korean black goats was separated into conventional and TMR diets treatments in a randomized complete block design. Conventional diet was based on forages and commercial diet. All animals were housed in individual metabolism crates and were fed ad libitum. Water was available at all times. Experimental period totally lasted for 38 days, consisting of 14 days of adaptation, 10 days of preliminary period and 14 days of sample collection period. Daily dry matter intake was significantly (p<0.05) higher in TMR treatments of the crossbred goats. Within breeds, crossbred rather than native-bred consumed more highly, and within the feeding type, TMR diets were more fed in comparison with conventional diet. Average daily gain ranked the highest in crossbred : TMR, followed by crossbred: conventional diet, native-bred: TMR, and native-bred: conventional diet (p<0.05). Dry matter digestibility was significantly (p<0.05) higher for TMR than for conventional diets, and it was higher in crossbred than in native-bred Korea black goats. Nitrogen retention was highest in TMR treatments of crossbreed, whereas it was lowest in conventional diet of native breed (p<0.05). Plasma glucose and urea nitrogen concentrations were significantly (p<0.05) higher in crossbred than in native-bred Korean black goats. Overall, the results indicated that feeding total mixed ration to crossbred rather than native-bred Korean black goats had more favourable influences on their performances, intakes, digestibility, nitrogen retention and blood metabolite characteristics. In conclusion, it is conceived that the results may contribute to economical benefit to farms producing Korean black goats together with establishment of an efficient feeding management system.

청보리 사일리지 급여 시에 농후사료 급여수준이 육성기 흑염소의 발육과 육질에 미치는 영향

본 시험은 청보리 사일리지 급여 시 농후사료 급여수준이 육성기 흑염소의 발육과 육질에 미치는 영향을 조사하였다. 시험은 흑염소 육성축 수컷 36두를 공시하여 대조구는 볏짚과 농후사료 수준을 체중의 2%로 급여하고 처리구는 청보리 사일리지와 농후사료 수준을 각각 체중의 2.0, 1.5 및 1.0%로 급여하여 2007년 5월 14일부터 2007년 10월 12일까지 시험을 실시하였으며 그 결과는 다음과 같다. 일당증체량은 대조구 보다 BS 1.5와 BS 2.0구가 유의하게 높게 나타났다(p<0.05). 1일 사료 섭취량은 대조구가 689.3g으로 BS 1.0구의 585.5g 보다 유의하게 높았지만 BS 2.0구의 734.3g 보다는 유의하게 낮게 섭취하였다(p<0.05). 도체율과 정육율은 BS 2.0과 BS 1.5구가 대조구와 BS 1.0구 보다 유의하게 높게 나타났다(p<0.05). 전단력과 보수력은 BS 2.0구와 BS 1.5구가 우수한 것으로 나타났으며(p<0.05), 관능검사는 청보리 사일리지 급여 시 농후사료 급여수준이 높을수록 우수한 경향으로 나타났다. 이러한 결과를 종합적으로 고려할 때 청보리 사일리지 급여 시 육성기 흑염소의 발육과 육질개선을 위한 농후사료 급여수준은 1.5%가 적정한 것으로 사료된다. Effects of dietary concentrate levels based on whole-crop barley silage (BS) on development and meat quality in growing Korean black goats were examined. A total of 36 male goats with similar age and BW were equally allocated to dietary four treatments in a randomized complete block design. Dietary treatments included controls(rice straw ad libitum plus 2.0% concentrate BW－1), and BS ad libitum plus three increasing levels of concentrate BW－1(2.0, 1.5 and 1.0%, respectively), expressed as BS 2.0, BS 1.5 and BS 1.0, respectively. The trial was lasted for 5 months from 14th of May to 12th of October, 2007. At the end of trial, all animals were slaughtered to analyze carcass characteristics and meat quality. Average daily gain was significantly(p<0.05) higher for BS 1.5 and BS 2.0 treatments than for controls. Daily feed intakes for controls(689.3 g) were significantly(p<0.05) higher than those of BS 1.0(585.5 g), but they were significantly(p<0.05) lower than BS 2.0(734.3 g). Carcass and meat percentages were significantly (p<0.05) higher for BS 2.0 and BS 1.5 than for control and BS 1.0 treatments. Shear force and water-holding capacity was greater(p<0.05) for BS 2.0 and BS 1.5 than other treatments. On the basis of feeding whole-crop barley silage to Korean black goats, as dietary concentrate inclusion levels increased, sensory results showed to have a better tendency. In conclusion, it was estimated that the optimal level of dietary concentrates might be 1.5% BW－1, when diets based whole-crop barley silage were fed to growing Korean black goats for their improvements of growth and meat quality.

사료의 에너지 수준이 육성기 흑염소의 발육과 육질에 미치는 영향

본 시험은 사료의 에너지 수준이 육성기 흑염소의 발육과 육질에 미치는 영향을 구명하기 위하여 흑염소 육성축 수컷 40두를 공시하여 대사에너지 수준을 각각 2.4, 2.6, 2.8 및 3.0 Mcal/kg로 하여 2006년 4월 20일부터 2006년 11월 6일까지 시험을 실시하였으며 그 결과는 다음과 같다.일당증체량은 대사에너지 2.4, 2.6, 2.8 및 3.0 Mcal/kg구가 각각 59.8, 65.4, 73.2 및 77.2g으로 사료의 에너지 수준이 높을수록 증가하는 경향이었고, 사료요구율은 각각 9.87, 8.88, 8.11, 7.19로 감소하는 경향이었다. 사료의 에너지 수준에 따른 도체특성은 에너지 수준이 증가함에 따라 도체율은 각각 49.73, 48.64, 50.19, 51.36%로 증가하는 경향이었으나 정육율, 지방율, 뼈율은 에너지수준 간에 차이가 없는 것으로 나타났다. 육질특성은 일반조성분인 조지방 함량은 사료의 에너지 수준이 증가함에 따라 증가하는 경향이었고, 물리적 특성인 전단력, 가열감량 및 보수력은 대사에너지 3.0 Mcal/kg에서 각각 2.82㎏/cm2, 29.6%, 59.0%로 다른 처리구보다 육질이 우수한 경향을 나타내었다. 이러한 결과를 종합적으로 고려할 때 육성기 흑염소의 발육과 육질개선을 위한 사료는 사료의 CP함량이 15%일 경우 대사에너지 수준은 ME 3.0 Mcal/kg이 적정한 것으로 사료된다. This study was conducted to investigate the optimal energy level at growing Korean black goat from April 20 to November 6, 2006. Forty male Korean black goat were divided into four treatments and were fed with concentrate feed containing ME 2.4, 2.6, 2.8 and 3.0 Mcal/kg with CP 15% and rice straw. Average daily gains of were 59.8, 65.4, 73.2 and 77.2 g/day in groups fed with ME 2.4, 2.6, 2.8 and 3.0 Mcal/kg diet, respectively. There was a tendency to increase ADG as the ME level of concentrate feed grew. Dressing percentage were 49.73, 48.64, 50.19 and 51.36% in each group, and were increased as the ME level grew. Meat percentage ranged between 53.1 and 55.3%, and were almost constant. Ranges of crude fat contents and water holding capacity were and 57.9~59.0%, which were increased as the ME level grew. On the other hand, shear force were between 2.8 and 3.1 kg/cm2, which were decreased as the ME level grew. These results suggest that the optimal energy level at growing goat's concentrate is ME 3.0 Mcal/kg.

섬유질배합사료 내 조단백질 수준이 임신초기 흑염소의 건물섭취량, 소화율 및 질소출납에 미치는 영향

This study was conducted to determine the effects of different levels (10, 12 and 15%) of crude protein (CP) in total mixed ration (TMR) on dry matter intake, digestibility and nitrogen balance of Korean black goats in the stage of early pregnancy and to obtain information on their optimal dietary levels of CP. In the present study, 12 Does of Korean black goats in the early pregnancy were allotted to four unreplicated groups by dietary level of CP and then they were housed in individual metabolism cages with completely randomized design throughout 30 days with 20 days adaptation and 10 days collection periods. Does in Control were fed a conventional diet and does in TMR10, TMR12 and TMR15 were fed a diet adjusted to about 10, 12 and 15% CP, respectively. Dry matter(DM) contents ranged from 89 to 91% in treatments. There were no differences fur fiber contents among three CP levels of TMR, showing that ADF and NDF had 18.57 to 19.85, and 53.41 to 54.80, respectively. Crude protein contents for three TMR treaements had 10.61, 12.15 and 14.97%, respectively. However, non-fibrous carbohydrate (NFC) contents decreased with increasing CP levels in treatments. Meanwhile, Intakes of DM, nutrients and digestible nutrients were significantly (p

산지 초지 유형이 번식 흑염소의 생산성 및 영양소 이용율에 미치는 영향

본 연구는 산림부산물과 국내 유기농부산물의 사료가치와 유기흑염소 생산에 기초적인 자료를 얻고자 산지 초지유형에 따른 흑염소의 사료 섭취량, 소화율 및 질소 축적율에 미치는 영향을 조사하였다. 공시축은 한국재래흑염소이었으며, 사양 시험에서는 흑염소 40두를 4처리구(개량목초구, 유기목초구, 산야초구, 수엽류구)로 나누어 처리구당 10두씩, 소화율 시험에서는 처리구당 3두씩 완전임의 배치하여 시험을 실시하였다. 그 결과는 다음과 같다. 사양시험기간 동안의 총증체량과 일당증체량은 개량목초구, 유기목초구, 산야초구, 수엽류구 순으로 낮아졌다(p<0.05). 건물섭취량, 가소화 건물섭취량, 건물소화율 및 질소축적율은 개량목초구가 가장 높았다(p<0.05). 이상의 결과를 종합해 보면, 산림 부산물을 조사료원으로 이용하고 국내 유기농부산물을 보충사료로 급여하여 방목사육된 유기흑염소는 다소 생산성이 낮으나 안정성이 높기 때문에 육용보다는 약용형태의 소비가 바람직하다고 사료되며, 낮은 생산성을 높이기 위해선 보충사료에 추가적인 영양소 공급이 반드시 필요하다고 판단된다. This study was conducted not only to evaluate the feed value for forests and organically produced-agricultural byproducts, but also to determine effects of different hilly pasture types on feed intake, nutrient digestibility and nitrogen retention in Korean black goats. Forty black goats were employed in the feeding trial and they were separated into four treatments (forages grown in chemical fertilizer, organic forages, native plants and browses) with 10 goats for each treatment. Furthermore, 12 goats, for nutrient digestibility trial, were allotted to few treatments of three goats per treatment with a randomized complete block design. Total body weight gain and average daily gain were significantly (p<0.05) decreased in the order of forages grown in chemical fertilizer, organic forages, native plants and browses throughout the trial. Dry matter intake (DM), digestible DMI, DM digestibility and nitrogen retention were highest in forages treatment grown in chemical fertilizer (p<0.05). From the above results, Organic Korean black goats fed forest byproducts as forage source and domestic organically produced-agricultural byproducts as supplemental diets were somewhat lowered in their performance. However, it showed high stability in the sustainable farming of organic Korean black goats. As the result, the consumption of organic Korean black goats might be more desirable for medicinal use rather than fur meat as a table food. The way to increase the low performance of organic Korean black goats for supplemental diets might be the additional supply of lacking nutrients for supplemental diets

가시오갈피 지엽의 급여가 흑염소의 생산성, 영양소 소화율 및 혈액성상에 미치는 영향

본 연구는 가시오갈피 지엽의 사료가치와 고품질 기능성 축산물 생산에 기초적인 자료를 얻고자 가시오갈피 지엽의 첨가 비율에 따른 흑염소의 사료 섭취량, 소화율, 질소 축적율 및 혈액성상에 미치는 영향을 조사하였다. 12두의 숫흑염소를 네 처리구로 나누어 처리구당 3두씩 완전 임의배치하여 개별대사케이지에 수용하였고, 실험기간은 21일간 지속되었다. 처리구는 대조구 및 가시오갈피 지엽을 10, 20 및 30%수준으로 첨가하여 네 처리구로 나누었다. 결과를 살펴보면, 시험사료의 화학적 조성분은 조단백질 함량이 대조구가 13.39%이었고, 가시오갈피 시험구는 첨가 비율이 증가함에 따라 조단백질 함량이 낮아졌다. ADF와 NDF 함량은 대조구가 가시오갈피 첨가구 보다 높은 경향이었고, NFC 함량은 가시오갈피 첨가구가대조구 보다 높은 경향이었다. 건물 섭취량은 시험구간 유의적 차이가 없었으나, 가소화 건물량에서는 가시오갈피를 20과 30% 첨가한 구가 다른 구에 비하여 유의하게 높게 나타났다(p<0.05). 대사체중당 건물 섭취량과 체중에 대한 건물섭취 비율은 가시오갈피 20% 첨가구가 가장 높았고, 가시오갈피 30% 첨가구가 가장 낮았다(p<0.05). 조단백질, ADF 및 NDF 소화율은 가시오갈피 첨가구가 대조구에 비해 높은 소화율을 보였고, 특히 가시오갈피 첨가 수준이 높을수록 높게 나타났다(p<0.05). 질소 축적량과 질소 축적율은 가시오갈피를 20과 30% 첨가한 구가 대조구 보다 유의하게 높게 나타났다.(p<0.05). 혈장내 요소태질소농도(PUN)는 가시오갈피 30% 첨가구에서 가장 낮았고, 혈장내 triglyceride는 가시오갈피 모든 첨가구가 대조구 보다 유의하게 낮았다(p<0.05). 이상의 결과를 종합하여 고려할 때 가시오갈피 지엽을 20% 수준으로 첨가시 소화율, 질소 축적, 혈장 내 요소태 질소 및 triglyceride 농도를 개선시켰고, 이는 흑염소의 생산성 향상에 잠재적으로 기여할 것으로 기대된다. This study was conducted not only to investigate effects of different supplementation levels of Acanthopanax senticosus leaves on feed intakes, nutrient digestibility, nitrogen retention, and blood metabolites in Korean black goats but also to obtain basic data for nutritive value of Acanthopanax senticosus leaves and production of high quality functional animal products. Twelve Korean black goats were allotted to treatments in four groups of three goats per treatment and then they were housed in individual metabolism cages with completely random arrangements for 21 days. Four treatments were separated into controls and three different supplementation levels of Acanthopanax senticosus such as 10, 20 and 30%, respectively. For the chemical composition of experimental diets, crude protein (CP) contents of controls were 13.39% and those of supplementation treatments were lowered with increasing levels of Acanthopanax senticosus. Control groups tended to have higher acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents as compared to supplementation groups, but non-fibrous carbohydrate (NFC) contents tended to be higher far supplementation groups than those for controls. Feed intakes had no significant difference among treatments, but digestible dry matter amounts were significantly higher for 20 and 30% supplementation groups than those for other treatments. Dry matter intakes per metabolic body weight and their ratio per body weight were highest in 20% group, but those of 40% groups were lowest (p<0.05). Crude protein, ADF and NDF digestibilities were significantly higher in Acanthopanax senticosus supplementation groups in comparison to controls (p<0.05), and in particular, they were increased with increasing supplementation levels of tranthopanax senticosus. Nitrogen retention was significantly higher for 20 and 30% treatments than those fur controls (p<0.05). Plasma urea nitrogen concentration was significantly lower for 30% supplementation group than those fur controls (p<0.05). Furthermore, plasma triglyceride concentration was significantly lower for Acanthopanax senticosus groups than those for controls (p<0.05). The results showed that supplemental level of 20% Acanthopnnax senticosus leaves to Korean black goats improved nutrient digestibility, nitrogen retention, and plasma urea nitrogen and triglyceride concentrations. Consequently, it might contribute to more efficient production of Korean black goats.

맥주박 첨가 섬유질 배합사료가 거세흑염소의 생산성 및 영양소 이용율에 미치는 영향

본 연구는 맥주박을 첨가한 섬유질 배합사료가 육성기 거세흑염소의 생산성과 영양소 이용율에 미치는 영향을 조사하여 맥주박의 사료가치를 평가하고자 실시하였다. 공시축은 사양시험에서는 거세흑염소 40두를 4처리구(관행사료구, 맥주박 20, 30 및 40% 첨가구)로 나누어 처리구당 10두씩, 소화율 시험에서는 처리구당 3두씩 완전임의 배치하여 시험을 실시하였다. 그 결과는 다음과 같다. 맥주박 첨가 수준별 섬유질 배합사료의 일반 조성분은 건물 함량이 <TEX>$62.35{\sim}66.02%$</TEX>, 조단백질 함량은 <TEX>$14.49{\sim}15.36%$</TEX>의 범위로 나타났으며, ADF와 NDF 함량은 <TEX>$28.24{\sim}29.08$</TEX> 및 <TEX>$53.27{\sim}54.85%$</TEX>로 나타나 맥주박 첨가 비율에 따른 섬유소 함량의 차이는 없었으나, NFC 함량은 맥주박 첨가 비율이 높을수록 NFC 함량이 낮아졌다. 일당증체량은 대조구가 맥주박 첨가구보다 유의하게 높았다(p<0.05). 건물섭취량, 유기물섭취량, NFC 섭취량은 맥주박 20% 첨가구가 가장 높았고(p<0.05), 조단백질과 가소화 조단백질 섭취량은 맥주박 첨가구가 대조구 보다 높은 경향이었다. 건물, 유기물, NDF 소화율은 대조구가 맥주박 첨가구 보다 유의하게 높았으며(p<0.05), 조단백질 소화율은 맥주박 첨가구가 대조구 보다 높은 경향이었고, 질소 축적율은 대조구 보다 맥주박 첨가구가 높은 경향이었다. This study was conducted to investigate the effects of Total Mixed Ration (TMR) with wet brewer's grain on feed intake, nutrient digestibility and nitrogen retention of castrated Korean black goat. For feeding trial, forty castrated Korean black goats were divided into four treatment groups, which were fed TMR containing 20, 30, 40% of wet brewer's grain. For digestibility trial, twelve castrated Korean black goats were allotted to treatments in four groups of three goats. Results are summarized as follows. Dry matter (DM) contents of TMR with wet brewer's grain was <TEX>$63.35{\sim}66.02%$</TEX>, that of crude protein was <TEX>$14.49{\sim}15.36%$</TEX>, Acid detergent fiber (ADF) and Neutral detergent fiber (NDF) were <TEX>$28.24{\sim}29.08,\;53.27{\sim}54.85%$</TEX>, each. These were not different by supplemental level of brewer's grain in TMR. However, Non-fibrous carbohydrate (NFC) contents were lower according to increase wet brewer's grain in TMR. Average daily gain of control group were higher than those from TMR with wet brewer's gain (p<0.05). Intake of DM, Organic matter (OM) and NFC of TMR supplemented with 20% of brewer's grain were higher than those of other treatments (p<0.05). Crude protein (CP) and digestible CP intake of TMR diets supplemented wet brewer's grain was intended to higher than those of a control group. Digestibilities of DM, OM and NDF in control group were higher than those from TMR with wet brewer's grain (p<0.05). Digestibilities of CP of TMR with supplemented wet brewer's grain was tend to be higher than those of a control group. Nitrogen retention of TMR supplemented wet brewer's grain was tend to be somewhat higher compared to the control group

Seroprevalence of Mycobacterium avium subspecies paratuberculosis in Korean Black Goats (Capra hircus aegagrus)

In total, 582 sera from 116 black goat herds were analyzed by a commercially available ELISA kit to monitor the seroprevalence of Mycobacterium avium subspecies paratuberculosis (Mpt) in Korean black goats (Capra hircus aegagrus). The mean number of goats sampled per herd was 5.11, 4.66, and 5.38 for the northern, central, and southern regions of Korea, respectively. The apparent regional prevalence of Mpt was estimated at 18.2-38.2% and 4.6-15.3% for herds and goats, respectively. The Mpt-positive goats were predominantly detected in the south (n=28), compared to either the northern (n=9) or central (n=11) regions (chi=14.459, P<0.05). Our findings indicate that Mpt is prevalent among the goat population, but regional variation exists.

Effects of feeding browses on growth and meat quality of Korean Black Goats

Effects of feeding browses on growth and meat quality of Korean Black Goats were investigated. Twenty-eight bucks were divided in equal numbers into four treatment groups and fed fibrous diets of oak browse, pine browse, fermented pine browse or rice straw each with concentrate supplement. Metabolic profile, growth rate, feed intake, carcass yield, meat quality and serum were investigated. Average daily gains of bucks were 45.3 g for oak browse, 36.4 g for rice straw, 28.1 g for pine browse and 30.0 g for fermented pine browse. Daily forage intake per goat was 197 g DM for oak browse, which was higher than those for the other diets. Bucks fed fermented pine browse ate only 74 g forage per day. Body metabolism was normal with all sources of browses from the results of serological inspection. Dressing percentage was 45.1–46.2%. Meat percentage was 55.2–55.9% and fat percentage was 7.31% for oak browse which was lower than those for pine browse and fermented pine browse. Shear force of goat meat was 4.11–5.11 kg/cm 2 for browses and 6.30 kg/cm 2 for rice straw. Cooking loss was 29.6% for pine browse which was lower than the others. Juiciness, tenderness and flavor of the goat meat were the best for the fermented pine browse followed by other browses and rice straw.

Ultrasonic Measurement of Fetal Parameters for Estimation of Gestational Age in Korean Black Goats

The objective of this study was to determine relationship of gestational age with measurement of diameter of head, orbit, trunk, long and short axis of heart, aorta, placentome, umbilical cord and umbilical vein in Korean black goats. In this study, ten pregnant Korean black goats (Capra hircus aegagrus) were used. Pregnancy diagnosis was performed with a 5 MHz linear transducer and ultrasonographic scan were performed at 60, 75, 90, 105, 120 and 135 days after mating with a 4-9 MHz convex transducer. For accurate measurement, all fetal organs were measured at least 3 times. The diameter of head, orbit, trunk, long and short axis of heart, aorta, placentome, umbilical cord and umbilical vein were significantly increased with the gestational age (p<0.05). Of these parameters, trunk (r=0.8876; p<0.001), long axis of heart (r=0.9168; p<0.001) and short axis of heart (r=0.8819; p<0.001) proved to be the more effective measurements than other parameters, as it correlated well with gestational age. Results indicate that ultrasonic measurements of these parameters were useful methods to estimate gestational age in Korean black goat.

182LAPAROSCOPIC EMBRYO TRANSFER IN KOREAN BLACK GOATS

In this study, laparoscopic embryo transfer (ET) was conducted to overcome the disadvantages of laparotomic ET including invasiveness, adhesions and duration of surgery in Korean black goats (Capra hircus aegagrus). Transferred transgenic embryos were produced by DNA pronuclear microinjection of in vivo derived zygotes. Recipient goats were synchronized in estrus by using an intravaginal progesterone device CIDR® for 13 days and injection of 400IU PMSG 48hrs before CIDR® removal. Embryos were transferred on day 4 after CIDR® removal. Recipient goats were starved for 48hrs and suspended head down in an operating table at an angle of 45° to the horizontal under general anesthesia. A Veress needle (Vomed, Tuttlingen, Germany) was inserted through the abdominal wall to make a pneumoperitoneum. After obtaining sufficient pneumoperitoneum, a 5-mm laparoscope and grasping forceps (MGB, Berlin, Germany) were inserted through the 5-mm trocar sleeves. After investigation of the ovaries, uterine horns and oviducts, embryos in a polyethylene tube (SP 65, Nastume, Tokyo, Japan) were transferred into the oviduct via the infundibulum in 76 recipients. To compare the pregnancy rates, laparotomic ET was also conducted in 21 recipients. In both groups, two microinjected embryos were transferred per recipient. Pregnancy of the recipient goats was examined by ultrasound on day 30 after embryo transfer. Pregnancy rates of laparoscopic ET were significantly higher than those of laparotomic ET (46.1% v. 28.6%; P&amp;lt;0.05). Our results suggest that laparoscopic ET is a highly efficient method for the transfer of goat embryos.

Potent and stage-specific action of glutathione on the development of goat early embryos in vitro.

The effect of glutathione (GSH) addition on the development of 1- or 2-cell goat early embryos in vitro was examined. Embryos were collected from superovulated Korean black goat (Capra hircus aegagrus) and cultured for 6 days in synthetic oviduct fluid medium supplemented with either bovine serum albumin (BSA) or serum. Without GSH addition, almost all embryos could not develop beyond 8- to 16-cell block. However, GSH addition greatly improved in vitro development of early embryos to blastocyst stage, and its action was highly dependent on the presence and source of proteins supplemented into the culture medium. Among the protein-supplemented cultures, GSH effect was most prominent in 10% FBS-supplemented culture, in which the proportion (91%) of blastocysts developed from early embryos was much higher than that of BSA- (42-64% depending on its content) or goat serum (GS)-supplemented cultures (21%), or even than that of somatic cell-supported co-culture (60%). As well as in terms of the morphological development, mean cell number of blastocysts (185 +/- 12) developed from FBS condition was significantly higher than that of blastocysts developed from any other culture conditions and moreover comparable to that of blastocysts developed in vivo (190 +/- 9). The viability of these blastocysts was finally confirmed by their term development (6/12) from embryo transfer. To delineate action time of GSH during embryo development, GSH was treated at 1-day intervals through 6-days culture periods excepting the last day. In the GSH-treated embryos at day 3 of culture, which corresponds to the time of in vitro 8- to 16-cell block stage, the proportion of blastocyst was markedly increased up to 77% of cultured embryos and conversely that of the arrested embryos was decreased to 7%. In the embryos treated later, however, their developmental potency decreased abruptly. Therefore, these results clearly demonstrated that GSH could greatly improve the in vitro development of goat early embryos by specifically acting on the 8- to 16-cell block stage during in vitro development, suggesting that GSH may be one of the important regulators on the development of goat embryos in vivo.