The purpose of this study is to [strengthen understanding of physical play and instructional ability] so that pre-primary teachers who hold the key to kindergarten education can have instructional abilities that can help early childhood children acquire basic motor skills. In addition, a physical education play program was operated for 15 sessions for 112 pre-infant/nursing teachers at K Junior College in Japan, aimed at ‘strengthening their understanding and instructional competence in sports play’. The confirmation of the effect of this physical education program was confirmed by the conscious and functional changes of the subjects before (2nd session) and after (15th session) implementation of the program. As a result, first, after participating in the sports play program, the awareness of participation in physical education increased, and the knowledge/information about the activity also increased by 10% to 30%, respectively. Second, with regard to the improvement of basic motion ability through the physical education play program, it was found that most of the three areas (average system exercise (3/4), movement system exercise (4/4), and manipulation system exercise (3/4) improved after implementation. Third, in the improvement of instructional professionalism/confidence through participation in the physical education play program and class composition presentation, all three items (understanding infant physical development, confidence in instructional physical education, will to learn instruction) significantly increased p<.000. As described above, it was confirmed that the physical education program practiced in this study was very effective in forming the physical education knowledge and instructional expertise of pre-primary early childhood teachers.