Background: The Needle stick Injuries (NSI) are among the incidents which risk the safety of health care workers in the hospital and healthcare setup. Health care professionals (HCP) are at greater risk of exposure to the blood and body fluids. Hepatitis B virus (HBV) transmission rate by NSI is estimated to be around 33.3%, followed by Hepatitis C virus 3.3% and HIV 0.3%. Aim of the study: assess of infection control knowledge, attitude and resources related to needle stick injuries among nursing staff. . Research design: Descriptive research design was used in this study. Setting: This study will be conducted at Beni suif University Hospital. Sample: includes all nurses in four critical care units in the hospital N= (68). Tool: three tools were used in the current study. First tool : knowledge regarding needle stick injury , second tool :Attitude of nurses toward needle stick injures and third tool : hospital resources. Results: the participated nurses had good knowledge and negative attitude toward needle stick injury and there are correlation between knowledge and their attitude and availability of hospital resources with needle stick injury . Conclusions: This study accentuates that there was significant correlation between knowledge and each of attitude and availability of hospital resources with needle stick injury .Recommendation: in-service training program for staff nurses about standard of infection control precautions. Healthcare institutions should apprise themselves of developments in hospital resources and safety devices, e.g. needle-less technology and reduce risk within their organization or modify work practices that pose a needle stick injury hazard to make them safer.