Objective: Irrational and overzealous use of antibiotics in addition to multidrug resistance has increased at an alarming limits around the world. This study aimed to evaluate the knowledge and practices toward antibiotic use among students at Yarmouk University
 Material and Method: A cross-sectional descriptive survey using a structured electronic version of valid questionnaire was distributed among participants. Google form was prepared based on available questionnaire in literature, revised by a group of academic pharmacist to validate the questionnaire. Test-retest was performed for a small group and cronbach alpha was calculated. The form was distributed randomly among under- and postgraduate students at Yarmouk University via their mails.
 Results: A total of 1154 individuals who completed questionnaires were analyzed. The knowledge of antibiotic use and resistance was quite good; 72.7% reported correct responses with a mean score of 16 out of 22. High rates of antibiotic use were found with 92% of respondents used antibiotics in the past three months. Inappropriate practice toward antibiotic was common; 48% used it for incorrect indication (e.g., common cold, fever, and pain), 60% used for improper duration and 20% don’t take the correct doses.
 Conclusions: Interventions on enhancing awareness and understanding of rational antimicrobial use are highly recommended by promoting expert-driven behavioural change, effective communication, education and training. Furthermore, law restriction on antibiotic dispensing should be introduced.