Low-Speed Pre-Ignition (LSPI) is a destructive combustion event associated primarily with new, ultra-efficient, downsized gasoline engines, which provide efficiency benefits in general operation. Biofuels, specifically bio-gasoline, are an alternative fuel that attempts to reduce the harmful emissions output by modern Internal Combustion Engines (ICEs). This study attempts to understand the effect of biofuel use on LSPI, through the use of a numerical simulation tool developed in Ricardo Wave. Development of the tool includes the integration of RFlame, an extension capable of modeling autoignition within a 1D domain. Use of the tool highlights the impact of five ethanol blends, E10, E20, E30, E50 and E85, with clear impacts on both the severity and frequency of LSPI events correlated with chemical properties, such as the enthalpy of vaporization (HoV) and octane number. E30 is highlighted as the critical blend for LSPI severity, with both increased severity and intensity seen with a 30% concentration, and a greater sensitivity to effects such as the start of ignition (SOI). Higher-concentration biofuels, such as E50 and E85 bio-gasoline, show much more favorable behaviors, such as a vast reduction in end-gas knock events, but are limited in their use due to their deployment being both cost-prohibitive and potentially damaging in current hardware. Future work on this topic will surround the further development of the simulation tool to integrate 3D solving elements, understand the role of fluid interactions in LSPI, and study optimal fuel characteristics for future use in ICEs.