Analysis of the mechanism of action of surfactants in the conditions of washing cotton knitted fabrics, the establishment of surfactants for functional purposes, which should be part of the composition, and the study of changes in the surface tension of solutions of surfactants of various classes designed to create a composition for the preparation of cotton knitted fabrics. Determination of the surface tension of solutions of surfactants of different concentrations was carried out by the method of detachment of the ring, using the Du-Nui method. The paper presents the results of the analysis of the mechanism of action of surfactants in the conditions of washing cotton knitted fabrics, it is established what properties the constituents of the composition should possess and the surfactants that contribute to the maximum reduction in the surface tension of their solutions are determined. Scientifically substantiated the principles of creating surfactants compositions for the scouring of cotton knitted fabric on the basis of an analysis of the mechanism of their action and found that the wetting and washing abilities of surfactants are the determinants; the isothermal dependences of the surface tension of solutions of surfactants of various classes are first obtained. Surface-active substances and their concentrations are established, which ensure low surface tension of solutions.