The purpose of the present study was to determine of the risk factors affected upon non-contact ACL injury in Gymnastics, Swimming and basketball collegiate student female players. If an athlete injured anterior cruciate ligament the most important thing is to identify factors that affect the injury. However, growing research implicates hormonal, anatomical, environmental and neuromuscular factors that may predispose athletes to these injuries. For this research selected 510 athletes for assess ACL injury factors by two different methods (A) Questionnaire and (B) Interview that information collected among athletes who were play Gymnastics, Swimming and basketball. Our results indicate 61.4% of ACL injury occurred in practice time and 38.6% of ACL injury was during competition. Also, our finding showed that 69% mechanism of injury was non-contact, 4.7% via accident and 26.3% with unknown reason. Furthermore, dynamic alignment at the time of the injury included: Knee-in & Toe- out 44%, Knee-out & Toe-in 22.5 and Hyperextension 11.1%. The ACL prevents the femur from moving forward during weight bearing. It also helps to prevent rotation of the joint. Injury of the ACL most often occurs when an athlete is pivoting, decelerating suddenly or landing from a jump. The injury can also be caused by another player falling across the knee. ACL injuries are, probably, the most common devastating knee ligament injuries amongst sports persons. Usually these injuries are isolated, mainly in non-contact sports, but many often are a part of more complex ligamentous injuries. They occur more often in contact sports, such as basketball. In conclusion, our study produced strong evidence in support of a significant some factors influencing of ACL injury in Gymnastics, Swimming and basketball collegiate student female players.