Conflicting evidence exists regarding the presence of aberrant gait biomechanics after the first year post-anterior cruciate ligament reconstruction. Overground walking may not elucidate differences in those further removed from surgery due to the unexacting nature of the task. Deleterious gait biomechanics following ACLR are partly attributable to quadriceps dysfunction. Downhill walking may exacerbate aberrant gait biomechanics, as this task places greater demands on the quadriceps compared to level walking. PURPOSE: To compare gait biomechanics between individuals with ACLR and healthy controls during level and downhill walking conditions. METHODS: 24 individuals more than 1 year removed from primary ACLR (83% female, age= 21 ± 3 yr, time since ACLR 44 ± 26 mo, BMI= 23 ± 3 kg/m2) and 24 healthy controls (79% female, age= 21 ± 1 yr, BMI= 24 ± 3 kg/m2) completed both level and downhill (10°grade) gait biomechanics assessments on an instrumented split-belt treadmill at their preferred walking speed. Peak variables were evaluated over the first 50% of stance including the vertical ground reaction force (vGRF), internal knee abduction moment, internal knee extension moment, knee flexion angle, and knee abduction angle. Moments were normalized to %body weight∗height (%BW∗Ht) and vGRF was normalized to %body weight. Dependent variables were compared across groups and conditions via two-way repeated measures ANCOVA controlling for gait speed. RESULTS: There were no significant condition∗group interaction effects nor group main effects for any outcomes. However, there were significant condition main effects for peak internal knee extension moment (p = 0.020, level to downhill mean increase of 0.042 %BW∗Ht) and peak knee flexion angle (p = 0.018, level to downhill mean increase of 13.2°). CONCLUSIONS: Downhill walking necessitates a larger internal extension moment and knee flexion angle compared to level gait. Our results suggest that changes in gait biomechanics between level and downhill conditions do not differ between individuals with ACLR > 1 year post reconstruction and controls. These results suggest that aberrant gait biomechanics may be mitigated over time in those with ACLR.