System Kit Research Articles

Developing Systemic Thinking through Gamification with Invention System Kits

Developing countries like Colombia have understood that education is an effective strategy in closing social inequality gaps to improve population’s skills. In the last decade, coverage in higher education went from 30 to 50 percent. One of the most important factors in this achievement is Colombia’s transition to peace, increasing the development of the population towards higher levels of competitiveness and education. In consequence, it is necessary to reinforce the development of competences, to encourage systemic thinking that allows the solution of problems from a holistic view and achieves effective solutions in the improvement of the local industry. During this study, an applied ludic strategy involving an airplane assembly line made with Lego blocks is created, looking for an effective and practical education framework in teaching the attributes that generate impact in a production line of goods; in this way, students can be involved in a clear and creative manner in their search for solutions. This project was developed by member professors and students from an engineering education institution in Bogotá, Colombia. The results show that through gamification, students develop skills to take decisions leading to increase the production’s competitiveness from a systemic thinking view.

Forensic features and genetic legacy of the Baloch population of Pakistan and the Hazara population across Durand line revealed by Y-chromosomal STRs

The Hazara population across Durand line has experienced extensive interaction with Central Asian and East Asian populations. Hazara individuals have typical Mongolian facial appearances and they called themselves descendants of Genghis Khan's army. The people who speak the Balochi language are called Baloch. Previously, a worldwide analysis of Y-chromosomal haplotype diversity for rapidly mutating (RM) Y-STRs and with PowerPlex Y23 System (Promega Corporation Madison, USA) kit was created with collaborative efforts, but Baloch and Hazara population from Pakistan and Hazara population from Afghanistan were missing. In the current study, Yfiler Plus PCR Amplification Kit loci were examined in 260 unrelated Hazara individuals from Afghanistan, 153 Hazara individuals, and 111 Balochi individuals from Baluchistan Pakistan. For the Hazara population from Afghanistan and Pakistan overall, 380 different haplotypes were observed on these 27 Y-STR loci, gene diversities ranged from 0.51288 (DYS389I) to 0.9257 (DYF387S1), and haplotype diversity was 0.9992. For the Baloch population, every individual was unique at 27 Y-STR loci; gene diversity ranged from 0.5718 (DYS460) to 0.9371(DYF387S1). Twelve haplotypes were shared between 178 individuals, while only two haplotypes among these twelve were shared between 87 individuals in Hazara populations. Rst and Fst pairwise genetic distance analyses, multidimensional scaling plot, neighbor-joining tree, linear discriminatory analysis, and median-joining network were performed, which shed light on the history of Hazara and Baloch populations. The results of our study showed that the Yfiler Plus PCR Amplification Kit marker set provided substantially stronger discriminatory power in the Baloch population of Pakistan and the Hazara population across the Durand line.

An Educational Training Kit for an Efficient GPS Anti-Jamming System

Global positioning system (GPS) is one of the key satellite-based radio technolo-gy providing navigational services on earth or near earth. The increasing usage of GPS applications in daily life has proved that students/researchers of many edu-cational departments including information and communication technology (ICT) are particularly interested to learn and visualize the GPS anti-jamming techniques in a simpler way in their major communication courses. It is a fact that GPS radio signal is vulnerable in various ways to jamming signals launched by malicious el-ements. The traditional available GPS anti-jamming techniques generally involve either added hardware and/or computational complexity which introduce difficul-ty for students to understand the basic concepts. As an alternative, this paper pre-sents a simpler GPS anti-jamming system kit based on L1-band (1.575 GHz) of GPS for education purpose. More specifically, the proposed antenna array, inte-grated with two couplers, cancels out the jamming signal in radio frequency (RF) domain using sum and difference patterns generated by the latter. The simulated and measured results, in terms of scattering parameters and power profiles, of the proposed system have shown that the jamming signal can be eliminated with less complexity. Eventually, the proposed GPS anti-jamming system has great poten-tial to be used in ICT related disciplines for learning and training purposes.

Detection of Listeria monocytogenes in Several Types of Frozen Meat in Baghdad city

Detection of pathogenic bacteria, such as Listeria monocytogenes, in food is crucial for safeguarding public health in Iraq. Forty five samples of frozen meat (15 samples of each of minced red meat, chicken, and fish) were collected from different markets in Baghdad city. Molecular (RT-PCR) and culturing (conventional microbiological examination) methods were used to determine the level of contamination of L. monocytogenes in these types of meat.
 For the culturing method, TSYEB broth was used as an enrichment medium, whereas BALCAM medium (HiMedia) with the listeria selective supplement FD061 was used as a selective medium, for the isolation and identification of this bacterium. The isolates were confirmed microscopically and biochemically. The results of the culturing method showed that the total number of the isolates of L. monocytogenes was 14/45 (31.1%). The incidence of this bacterium was high in fish (11/15, 73.3%), while it was low in the other two types of meat. 2/15 (13.3%) in red meat and 1/15 (6.7 %) in chicken.
 Molecular detection of each sample of the bacteria was performed using RT-PCR technique after preparing the Genomic DNA extraction of these samples according to the protocol provided by ReliaPrep™ Blood gDNA Miniprep System kit (Promega, USA). The PCR primers and the hybridization probe ((Macrogen, Korea) were used to target the inlA gene sequence (specific for L. monocytogenes). The results of the RT-PCR assay showed that 10/45 (22.2%) of the samples were positive for L. monocytogenes, which was detected only in fish samples ((10/15, 66.7%), while not found in minced red meat and chicken. However, our results showed differences when compared to other previous works because there were many studies found that the highest contamination rate was in red meat products.
 We conclude that the PCR kit used for the detection of L monocytogenes appears to give accurate results in the diagnoses of this bacterium in meat products and in comparison with the other routine diagnosis methods in the laboratory, which included culturing and doing biochemical tests which last for approximately 7 days, the RT-PCR technique was able to confirm the findings within 48 hours.

Phylogenetic Analysis and Extracellular Enzyme Profiles of Yeast Strains Isolated from Raspberry Fruits

Raspberry fruit contains phenolic compounds, flavones, flavonoids, vitamins, and antioxidant substances that are important properties for health and pharmacological sciences. Edible berries provide also a suitable habitat for the growth of various microorganisms. In this study, yeast biota associated with raspberry fruits was determined by molecular identification techniques. Raspberry fruits were collected from Çanakkale, Gelibolu (Gallipoli). Yeast strains were isolated and then identified by using the analysis of ITS1-5.8S-ITS2 rDNA gene sequences. The phylo-genetic analysis of all yeast strains was carried out by using the MEGA–X phylogenetic analysis tool. The extracel-lular enzyme profiles of identified yeast species were determined by the API-ZYM kit system. The distribution of yeast species on the raspberry fruits was determined as Hanseniaspora uvarum, Metschnikowia viticola, Aureo-basidium pullulans, and Metschnikowia pulcherrima. It was observed that yeast strains belong to Metschnikowia genus were dominant on raspberry fruits. All yeast strains in Metschnikowia genus showed different enzyme pro-files against seven extracellular enzymes. These enzymes may be the discriminatory enzymes for the yeast strains in the Metschnikowia genus. When the phylogenetic relationships among all yeast strains were investigated, all strains were divided into two main clades. While the first clade consists of only Metschnikowia genus, second clade includes H. uvarum and A. pullulans yeast species. Our results indicated that restriction patterns and also extracel-lular enzyme profiles could be utilized for differentiation of yeast strains within the genus. M. pulcherrima, H. uvarum, and A. pullulans can be used for industrial applications for future researches.

Direct Saliva versus Conventional Nasopharyngeal Swab qRT-PCR to Diagnose SARS – CoV2: Validity Study

Background: Saliva has been demonstrated as a feasible alternative specimen to nasopharyngeal swab for the detection of SARS-CoV-2 using real-time or quantitative reverse transcription polymerase chain reaction (qRT-PCR) method that bypasses the need for explicit viral ribonucleic acid (RNA) extraction.
 Aim: To assess the diagnostic validity of direct saliva-to-qRT-PCR in the detection of SARS-CoV-2 compared to conventional nasopharyngeal swab qRT-PCR.
 Methodology: Self-collected saliva samples were processed by heating at 95oC for 30 minutes followed by addition of buffer and detergent while viral RNA from nasopharyngeal swabs were extracted using the Sansure Biotech sample release reagent. Paired samples were used as templates for qRT-PCR using the Sansure Novel Coronavirus (COVID-19) Nucleic Acid Diagnostic Kit and Sansure Biotech MA6000 Real-Time Quantitative PCR System. Direct saliva-to-qRT-PCR was compared to nasopharyngeal swab qRT-PCR in terms of diagnostic validity and agreement parameters, and both platforms were compared separately in terms of similar parameters with a composite reference standard (CRS) wherein the criteria for a positive result is SARS-CoV-2 detection in at least either nasopharyngeal swab or saliva.
 Results: Of the 238 nasopharyngeal swab-saliva pairs tested, 20 (8.4%) nasopharyngeal swab and 24 (10.1%) saliva specimens tested positive. We documented a sensitivity of 85.0% (95% CI: 62.1%, 96.8%), specificity of 96.8% (95% CI: 93.5%, 98.7%), accuracy of 95.8% (95% CI: 92.4%, 98.0%) and Cohen Kappa of 0.75 (95% CI: 0.60, 0.90) when direct saliva-to-qRT-PCR was compared to the conventional platform. When the two platforms were individually compared to the CRS, numerically higher but not statistically significant sensitivity and accuracy were noted for direct saliva-to-qRT-PCR than for nasopharyngeal swab qRT-PCR.
 Conclusion: Direct saliva-to-qRT-PCR is non-inferior to nasopharyngeal swab qRT-PCR for detecting SARS-CoV-2 using the Sansure Novel Coronavirus Nucleic Acid Diagnostic Kit.

British Racing Green

The British Touring Car Championship Is Embracing Hybrid Power For The First Time From 2022, Which Sees Cosworth And Delta Motorsport Lead The Race Series Evolution With The Development Of A Hybrid Kit And Specially-Created Battery System

Discussion on the Terms of Idea Managament and Idea Managament Systems

Although idea management (IM) and idea management systems (IMS) and related topics have been researched since 1982 and IM itself is as old as the first ideas, authors in previous research specified 18 research gaps in literature about IM and one of the most important that there is no united view on the terms IM and IMS and there are no specialized researches about these terms, as well, in the literature of IM they are not board discussed. Purpose of the research is to discuss and create definitions of terms IM and IMS using the reviewed literature. Research object: literature on IM and IMS. Research subject: terms of IM and IMS. Main research method – literature review. Data collected from 70 sources, including scientific publications, conference proceedings, books, white papers etc. published over the last 33 years, i.e., from January 1982 to February 2015, in all research fields. The research highlights that in the literature there are wide variety of the definitions of terms IM and IMS. The results of the research revealed that definitions of IM have not fundamentally changed over the time, but the definitions of IMS have transformed from “passive” to “active” IMS in millennium. The object of these definitions more often are described as systematic and manageable. Results probe that IM could be defined as – systematic, manageable process of idea generation, evaluation and development, but the IMS as a tool, tool kit or complex system which provides systematic, manageable process of idea generation, evaluation and development. The intensity of IM and IMS researches varies by region, but there are no major regional differences in defying the terms.KEYWORDS: Idea management, Idea management systems, Discussion, Literature review.JEL CODE: M190; M150; M110

Probeware for the Modern Era: IoT Dataflow System Design for Secondary Classrooms

Sensor systems have the potential to make abstract science phenomena concrete for K-12 students. Internet of Things (IoT) sensor systems provide a variety of benefits for modern classrooms, creating the opportunity for global data production, orienting learners to the opportunities and drawbacks of distributed sensor and control systems, and reducing classroom hardware burden by allowing many students to “listen” to the same data stream. To date, few robust IoT classroom systems have emerged, partially due to lack of appropriate curriculum and student-accessible interfaces, and partially due to lack of classroom-compliant server technology. In this article, we present an architecture and sensor kit system that addresses issues of sensor ubiquity, acquisition clarity, data transparency, reliability, and security. The system has a dataflow programming interface to support both science practices and computational data practices, exposing the movement of data through programs and data files. The IoT Dataflow System supports authentic uses of computational tools for data production through this distributed cloud-based system, overcoming a variety of implementation challenges specific to making programs run for arbitrary duration on a variety of sensors. In practice, this system provides a number of unique yet unexplored educational opportunities. Early results show promise for Dataflow as a valuable learning technology from research conducted in a high school classroom.

Abstract PS5-26: Pd-l1 mRNA expression in plasma-derived exosomes predicts prognosis and response to anti-pd-l1 antibodies in unresectable locally advanced triple negative breast cancer (TNBC) treated with atezolizumab-paclitaxel

Abstract Background Despite modern therapies have improved outcomes of patients with non-TNBC cancers, patients diagnosed with unresectable locally advanced TNBC usually have poor outcome for its aggressive clinical behaviour. Over time for cancer management, predicting and monitoring response to treatments is crucial, better if through easy non-invasive procedures. Atezolizumab plus nanoparticle albumin-bound (nab)-Paclitaxel prolonged progression-free survival (PFS) and overall survival (OS) among patients with TNBC, as demonstrated by phase III IMpassion130 trial. Despite this, there is a dire need for biomarkers reflecting treatment response. In our study, we correlated plasma PD-L1 mRNA levels with prognosis and response to anti -PD-L1 antibodies in unresectable locally advanced TNBC in the era of Atezolizumab. Methods We assessed 30 consecutive patients with unresectable locally advanced TNBC treated with Atezolizumab plus nab-Paclitaxel. Blood samples were collected at time point 0 (at baseline) and after 2 months and we assessed the association between PD-L1 mRNA copies per ml in plasma-derived exosomes and response to treatment. Exosomal PD-L1 mRNA in plasma was determined using Bio-Rad QX100 digital droplet PCR system and exoRNeasy kit. Objective responses were defined following the RECIST criteria v.1.1 Results At baseline, patients with complete and partial response (CR+PR, n=11) displayed a significantly higher number of PD-L1 mRNA copies per ml compared to ones with stable or progressive disease (SD+PD, n=19) (mean value: 745.6±131.1 vs 124.7±34.4, p<0.001). In patients with CR and PR mean PD-L1 copies/ml were 745.6±131.1 and 175.4 at baseline vs 2 months, respectively (p=0.001).In patients with stable disease the mean ± s.e.m. values were 270±71.1 vs 217.5±17.3 copies per ml (p=0.614) while in progressive disease PD-L1 mRNA levels were 112.1±31.2 vs 454.3±46.2 copies per ml at baseline vs 2 months, respectively (p<0.001).Two months after the start of Atezolizumab plus nab-Paclitaxel decrease of PD-L1 mRNA copies per ml relative to pre-treatment PD-L1 mRNA copies per ml, was positively correlated with an objective response to treatment.Increase of PD-L1 mRNA copies per ml was significantly associated with worse and shorter OS: median OS in patients with increase of PD-L1 mRNA copies/ml was 5 months (range 2-7 months, 95%CI 1.1-6.1); on the other side median OS in patients with decrease of PD-L1 mRNA copies/ml was more than double (range 8-15 months) (p<0.001).Moreover, prolonged survival outcomes (p<0.001) were recorded in cases with low baseline circulating Lox1+ Myeloid-derived suppressor cells (MDSC) (r=0.62, p<0.001) and high Natural Killer cells (NK) (r=0.66, p<0.001): the NK-to-MDSC ratio (NMr) was significantly higher in patients with higher number of PD-L1 mRNA copies per ml at baseline (p=0.002). During PD-L1 blockade, 2 months after starting treatment, NK progressively raised in clinical benefit group while declined in non-responders (p=0.001) Conclusions Despite the study’s limitations, our data suggest exosomal PD-L1 is significantly associated with outcome and response to treatment. Citation Format: Lucrezia Raimondi, Laura Di Benedetto, Victoria Bitca, Gian Paolo Spinelli. Pd-l1 mRNA expression in plasma-derived exosomes predicts prognosis and response to anti-pd-l1 antibodies in unresectable locally advanced triple negative breast cancer (TNBC) treated with atezolizumab-paclitaxel [abstract]. In: Proceedings of the 2020 San Antonio Breast Cancer Virtual Symposium; 2020 Dec 8-11; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2021;81(4 Suppl):Abstract nr PS5-26.

Maternal allele mutation: Slippage synthesis furnishing evolutionary trend

Gain or loss of repeat motifs leads to an allelic mismatch in the disputed child which is a deviation from the Mendelian inheritance, thereby leading to a paternal mismatch of putative father or exclusion of mother in case of maternal allelic mismatch. This allelic mismatch at one or more loci is a major cause of forensic inferences. The biological samples of the case were genotyped using Powerplex®- Fusion 5C system kit and Investigator® Argus X-12 QS kit as per recommendations of the manufacturer. In this case, identification of a putrefied dead body with 22 autosomal STR loci, primarily analyzed by Powerplex®- Fusion 5C system kit divulged a maternal mismatch at locus D13S317. Alleles at the locus D13S317 allegedly belonging to the father of the deceased and the mother were observed as 10/11, 11/11 and 10/10 respectively. To rule out allelic mismatch at this particular locus, 12 X-STR loci were amplified, in which all the maternal alleles of deceased completely matched with the mother. This case study indicates the extension of one microsatellite repeat motif (TATC) at locus D13S317 in the population of Rajasthan. The reported mutation rate was 0.14% and 0.04% at locus D13S317 in paternal and maternal meiosis respectively.

Improving the Fatigue of Newly Designed Mechanical System Subjected to Repeated Impact Loading

This paper develops parametric accelerated life testing (ALT) as a systematic reliability method to produce the reliability quantitative (RQ) specifications—mission cycle—for recognizing missing design defects in mechanical products as applying the accelerated load, expressed as the inverse of stress ratio, R. Parametric ALT is a way to enhance the prediction of fatigue failure for mechanical systems subjected to repeated impact loading. It incorporates: (1) A parametric ALT plan formed on the system BX lifetime, (2) a fatigue failure and design, (3) customized ALTs with design alternatives, and (4) an assessment of whether the last design(s) of the system fulfills the objective BX lifetime. A BX life concept with a generalized life-stress model and a sample size equation are suggested. A domestic refrigerator hinge kit system (HKS), which was a newly designed mechanical product, was used to illustrate the methodology. The HKS was subjected to repeated impact loading resulting in failure of the HKS in the field. To conduct ALTs, a force and momentum balance was utilized on the HKS. A straightforward impact loading of the HKS in closing the refrigerator door was examined. At the first ALT, the housing of the HKS failed. As an action plan, the hinge kit housing was modified by attaching inside supporting ribs to the HKS to provide sufficient mechanical strength against its loading. At the second ALT, the torsional shaft in the HKS made with austenitic ductile iron (18 wt% Ni) failed. The cracked torsional shaft for the 2nd ALTs came from its insufficient rounding, which failed due to repeated stress. As an action plan, to have sufficient material strength for the repetitive impact loads, the torsional shaft was reshaped to give it more rounding from R0.5 mm to R2.0 mm. After these modifications, there were no problems at the third ALT. The lifetime of the HKS in the domestic refrigerator was assured to be B1 life 10 years.

Point-of-care test system for detection of immunoglobulin-G and -M against nucleocapsid protein and spike glycoprotein of SARS-CoV-2.

The coronavirus disease 2019 (COVID-19) epidemic continues to ravage the world. In epidemic control, dealing with a large number of samples is a huge challenge. In this study, a point-of-care test (POCT) system was successfully developed and applied for rapid and accurate detection of immunoglobulin-G and -M against nucleocapsid protein (anti-N IgG/IgM) and receptor-binding domain in spike glycoprotein (anti-S-RBD IgG/IgM) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Any one of the IgG/IgM found in a sample was identified as positive. The POCT system contains colloidal gold-based lateral flow immunoassay test strips, homemade portable reader, and certified reference materials, which detected anti-N and anti-S-RBD IgG/IgM objectively in serum within 15 min. Receiver operating characteristic curve analysis was used to determine the optimal cutoff values, sensitivity, and specificity. It exhibited equal to or better performances than four approved commercial kits. Results of the system and chemiluminescence immunoassay kit detecting 108 suspicious samples had high consistency with kappa coefficient at 0.804 (P < 0.001). Besides, the levels and alterations of the IgG/IgM in an inpatient were primarily investigated by the POCT system. Those results suggested the POCT system possess the potential to contribute to rapid and accurate serological diagnosis and epidemiological survey of COVID-19.

Reliability design of mechanical systems subjected to repetitive stresses

To enhance the design of mechanical systems, parametric Accelerated Life Testing (ALT) as a systematic reliability method is proposed as a way to evaluate the design of mechanical systems subjected to repeated impact stresses. It requires: (1) a parametric ALT scheme shaped on system BX lifetime, (2) a load inspection, (3) parametric ALTs with the associated design modifications, and (4) an assessment of whether the revised product design(s) reach the targeted BX life-time. We propose using a general life-stress model and sample size equation. A test example using both market data and parametric ALT was the redesign of a hinge kit system (HKS) in a refrigerator. To conduct parametric ALTs, a force and moment balance analysis was utilized. The mechanical impact loadings of the HKS were evaluated for an working refrigerator door. For the first ALT, the HKS failure happened in the crack/fracture of the kit housing and oil spilled from the damper when the HKS was disassembled. The failure modes and mechanisms constructed in the 1st ALT were similar to those of the unsuccessful samples found from the marketplace. The missing design parameters of the HKS included stress raisers such as corner roundings and the rib of the housing in HKS, the seal in the oil damper, and the material of the cover housing. In the second ALT, the cover housing fractured. The design defect of the cover housing in the HKS was the plastic material. As a corrective action plan, the cover housing was modified from plastic to aluminium. After the second ALT, the lifetime of the modified HKS was reassured to be B1 life 10 years with a yearly failure rate of 0.1%.

Pani Addition to Improve Contact Between CdTe/CdS Semiconductors

This paper presents the study of the addition of the polyaniline polymer (PANI), at the junction of the cadmium telluride and cadmium sulfide (CdTe/CdS) layers in order to improve the contact between the layers, to decrease the air gap that limit the conversion photovoltaic. The morphology of CdS, CdTe and polyaniline (PANI) were characterized with multiple techniques including scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD) and optical absorption. The Metrohm Autolab LED Driver Kit system was used for data collection, and it was possible to obtain the current and voltage parameters of the cells. The inclusion of PANI thin film in CdS/CdTe hybrid solar cells increases the energy conversion efficiency from 0.0075% to 0.15%, this gives a 20-fold increase in efficiency.

Antimicrobial Susceptibility and Minimum Inhibitory Concentration of &amp;lt;i&amp;gt;Salmonella enterica&amp;lt;/i&amp;gt; Isolates from Chickens in Yobe State

<i>Salmonella</i> of poultry are zoonotic microorganisms transmitted to humans and other animals via contact with infected poultry feces, meat, eggs and formites. This study was conducted to phenotypically characterize <i>Salmonella enterica</i> from samples collected from chickens presented for slaughter in some selected Local Government Areas of Yobe State, Nigeria, as well as carry out antimicrobial susceptibility and minimum inhibition concentration on the isolates. A cloacal swab and blood samples were collected and transported on ice pack to Veterinary Microbiology Laboratory, University of Maiduguri and analyzed for the presence of <i>Salmonella enterica</i>. Samples were then inoculated onto Xylose Lysine Deoxycholate agar for morphological identification of <i>Salmonella</i> blackish colonies. A total of 600 (300 cloacal swab and 300 blood), consisting (202 males, 98 female chickens, 150 local and exotic each) were randomly sampled in 16 weeks for the isolation of <i>Salmonella enterica</i>. The presumptive <i>Salmonella</i> isolates were further characterized using the Microbact<SUP>TM</SUP> GNB 24E System kit, with 40 randomly selected presumptive isolates (8 from blood and 32 from cloacal swab) tested using Microbact 24E GNB Computerize system, with 10 samples found to be positive for <i>Salmonella</i> organisms out of which 9 (22.5%) were from cloacal swab and 1 (2.5%) from blood. All the blood samples were tested for haemagglutination using slide method, 255 were found to be positive, where agglutination was observed. Where as only 8 (2.7%) were positives after blood culture 8 (2.67%). Exotic chickens showed the highest resistance level of (35%) to commonly used antibiotics (Amoxixillin and Ampicillin). The isolates from exotic chickens are susceptible to Ciprofloxacin 11 (68.8%), Ofloxacin 10 (62.5%), Gentamicin 2 (12.5%), Levofloxacin and Erythromycin 6 (37.5%), while intermediate to Norfloxacin 5 (31.3%) and Amoxicillin 7 (43.8%) but were resistant to Ampicillin 6 (37.5%%), Cefuroxime 10 (62.5%) and Amoxicillin 4 (25.0%). The MIC was carried out on all the 10 <i>Salmonella</i> isolated that showed positive on microbact 24E computerized system. All the 10 isolates from microbact 24E computerized system showed susceptibility to amoxicillin, ofloxacin, and ciprofloxacin on MIC. The MIC of ofloxacin and ciprofloxacin was distributed within 0.00175–2 µg/ml each, and for amoxilin, the MIC ranged between 0.00175-3.00 µg/ml. It is therefore, concluded that <i>Salmonella</i> organisms phenotypically characterized in the study area had antimicrobial susceptibility to routinely used antimicrobial drugs. As a result, it is suggested that the medications with high susceptibility be used to treat poultry salmonellosis in the study area.

КОНВЕРТАЦІЯ ФОРМАТІВ ЗБЕРІГАННЯ ГРАФОВИХ БАЗ ДАНИХ І ЇЇ РОЛЬ В ОНТОЛОГІЧНОМУ ІНЖИНІРИНГУ НА ПРИКЛАДІ СИСТЕМИ СУПРОВОДЖЕННЯ РЕАБІЛІТАЦІЇ

КОНВЕРТАЦІЯ ФОРМАТІВ ЗБЕРІГАННЯ ГРАФОВИХ БАЗ ДАНИХ І ЇЇ РОЛЬ В ОНТОЛОГІЧНОМУ ІНЖИНІРИНГУ НА ПРИКЛАДІ СИСТЕМИ СУПРОВОДЖЕННЯ РЕАБІЛІТАЦІЇ

Improving the Lifetime of Mechanical System Such as Hinge Kit System Subjected to Repeated Impact Stress

To enhance the lifetime of mechanical system, parametric Accelerated Life Testing (ALT) as new systematic reliability method suggests evaluating the design of mechanical systems subjected to repeated stresses, based on failure mechanism and design. This newly developed parametric ALT helps an engineer uncover the missing design parameters (or design flaws) of the mechanical system having an effect on reliability during the product developing process. As a result, companies can circumvent product recalls due to the design flaws from the marketplace. As an experiment instance, the redesign of hinge kit system (HKS) in a domestic refrigerator was investigated.

Evaluation of long-term storage effects on buccal cell DNA from untreated cards for STR profiling

The success of DNA profiling using long-term stored samples depends on the amount and quality of their recovered DNA templates. Physical and biochemical factors such as microbial activity, humidity and temperature contribute to DNA degradation. In this study, we used genomic DNA extracted from buccal cell samples that were stored for more than 4 years on Bode Buccal DNA Collector™ cards (Bode Technology, Virginia, USA) for typing 27 and 24 short tandem repeat (STR) loci using Powerplex® Fusion 6C system (Promega Corporation, Madison, USA) and Globalfiler™ Express kit (Thermo Fisher Scientific, USA), respectively. Our results demonstrated that the Buccal DNA Collector can be used as a collection medium for buccal swab samples that are not immediately analyzed or those that need to be retrospectively analyzed. There is a sign of DNA degradation which might well be expected because buccal cell samples were deposited on untreated filter paper and have been stored for a long period (> four years) at room temperature. However, STR allele calls were obtained from most of the buccal cell samples, especially when typed using the Powerplex® Fusion 6C system kit.

IoT Architecture based on Information Flow Diagram for Vermiculture Smart Farming Kit

IoT technology is widely applied to many areas, including agriculture. The smart farming design and implementation deal with farm operations and management effectively. The aim of this research enables supporting a vermiculture smart farming kit based on IoT technology. The layered architecture design is represented to support the deployment of sensors, networks, monitoring systems, data collections, and watering decision system. Information flow diagram is proposed to improve how the web application of our smart kit system can implement based on the system requirements. The evaluations of the smart kit are investigated to deal with consistency and effectiveness comparing between a traditional and the smart kit of earthworm vermicomposting. The gaps of farmers' needs can be satisfied to advance the better solutions of the smart farming kit.