Kinetic Analysis Research Articles

Non-isothermal kinetic analysis of phase transformations in Fe-Co-V-Mo semi-hard magnetic alloy

In this study, the non-isothermal kinetics and phase transformations within the Fe-Co-V-Mo alloy were investigated. Four distinct transformations were identified: disorder → order (T1), first-stage polymorphic (T2), order → disorder (T3), and second-stage polymorphic (T4). The activation energy (E) for each transformation was determined using isoconversional methods. Fitting model were employed to calculate the kinetic triplets, confirming that all transformations follow the Avrami model. The Johnson-Mehl-Avrami (JMA) and Šesták-Berggren (SB) models were used to determine other kinetic parameters (n, M, and N). The findings suggest that the growth mechanisms for transformations T3 and T4 are interface-controlled, whereas transformation T2 is diffusion-controlled. Consequently, the A3/2 and A3 mechanisms were identified as predominant mechanisms for transformations T2 and T4, respectively. Additionally, transformation T3 follows the A3 mechanism at heating rates of 10 and 20 K/min, and the A2 mechanism at 30 K/min. Kinetic analysis revealed that the addition of Mo in Fe-Co-V alloys, acting as a ferrite (α) stabilizer, decreases the onset temperatures of transformations T1 and T3. Conversely, it increases those of transformations T2 and T4. Furthermore, Mo influences the reduction of E associated with transformation T3.

Oxidative pyrolysis for enhanced-CO2 adsorption capacity in biosolid-derived biochar

The study conducts the adsorption kinetic analysis of biosolid-derived biochar produced from pyrolysis and oxidative pyrolysis. The adsorption kinetic analysis is performed at three different temperatures. The characteristics of the adsorption and diffusion mechanisms are evaluated by applying adsorption kinetic models and diffusion mechanism models. The pseudo-first-order model (PFO) and the pseudo-second-order model (PSO) reveal that the CO2 adsorption process of the biosolid can be categorised as physisorption with activation energy below 40 kJ/mol. The CO2 adsorption capacities of the biochar produced at 700°C, 800°C, and 900°C are 6.3, 7.9, and 6.4 mg/g at 45°C, respectively. In contrast, the biochar produced from oxidative pyrolysis shows a CO2 adsorption capacity of 7.5 mg/g at 45°C. Film and intraparticle diffusions are primary rate-limiting factors of the adsorption process. The biochar samples maintain 84-85% of their adsorption capacities after five cyclic tests. The present study demonstrates the CO2 adsorption capacity of biosolid-derived biochar produced from different conditions of pyrolysis, providing an energy-efficient and sustainable solution to CO2 adsorption with solid adsorbents.

Structural characterization of noni (Morinda citrifolia L.) pectin and its inhibitory activity on pancreatic lipase

This study aimed to investigate the physicochemical properties and biological activities of noni pectin (NP) extracted using various solvents, three fractions of noni pectin were obtained using traditional chemical and deep eutectic solvents. NPs are composed of various ratios of galacturonic acid, arabinose, rhamnose, xylose, glucose, and galactose with different yields, molecular weights, esterification degrees (DE), and microstructures. Among the fractions, noni pectin extracted with Betaine-citric acid (BNP) exhibited higher molecular weight, degree for esterification and pancreatic lipase (PL) inhibitory activity than the other fractions. Enzyme kinetic analysis indicated that BNP inhibited PL via a noncompetitive mechanism. BNP significantly altered the secondary structure of PL and quenched PL fluorescence, suggesting a static quenching mechanism. Isothermal titration calorimetry (ITC) further demonstrated that the binding of BNP to PL was spontaneous and driven by enthalpy, primarily mediated by hydrogen bonding and van der Waals forces. Molecular docking simulations also confirmed strong noncovalent interactions (hydrogen bonding and van der Waals forces) between BNP and PL. This study validated the high efficiency of the deep eutectic solvent-extracted noni pectin fraction in pancreatic lipase inhibition.

Assessing the discrepant role of anions in the transformation of reactive oxygen species in H2O2 and PDS system: A comparative kinetic analysis

Clarifying reactive oxygen species (ROS) variation in the presence of co-existing anions is significant for understanding the catalytic effect of magnetite (Fe3O4)-induced advanced oxidation processes (AOPs) in natural environment, yet this remains controversial. Herein, we compare the specific impacts of NO3-, SO42-, and Cl- on ROS (•OH, SO4•-, O2•-, and 1O2) exposure concentration in H2O2 and peroxydisulfate (PDS) systems, as well as how these variations affect the catalytic efficiency by developing kinetic model. In both two systems, NO3- demonstrates no discernible effect on ROS, whereas SO42- inhibits the exposure of all ROS and thus micropollutants degradation. Through theoretical calculation, it is proposed that SO42- primarily exerts its influence through affecting the electronic structure over catalyst surface. Regarding Cl-, it affects ROS exposure mainly by reacting with ROS. It shows inhibitory effect on 1O2 in both systems, but its suppressive impact on •OH is markedly more pronounced in H2O2 system compared to PDS system, which may be related to its rapid reactivity with SO4•-. Besides, the chlorine radicals (mainly ClO•) generated through the reaction of Cl- may exert a selective influence on micropollutants degradation. This study can help to re-understand the influence behavior of co-existing anions during AOPs.

Biochemical and steady-state kinetic analyses of arsenate reductases from an arsenic-tolerant strain of Citrobacter youngae IITK SM2

Biochemical and steady-state kinetic analyses of arsenate reductases from an arsenic-tolerant strain of Citrobacter youngae IITK SM2

Is beudantite a stable host phase of arsenic and lead? New insights from molecular-scale kinetic analyses

Is beudantite a stable host phase of arsenic and lead? New insights from molecular-scale kinetic analyses

Comparative study of pre- and post-Cu modified calcium-based desulfurizers: experimental and theoretical insights into adsorption vs. catalysis

This study focused on the dry desulfurization of calcium-based materials, with the aim of conducting an in-depth investigation into the mechanistic differences in desulfurization between traditional commercial calcium-based materials and their Cu-modified counterparts, utilizing experiments, reaction kinetics, and density functional theory (DFT). Through a fixed-bed reactor, factors affecting desulfurization efficiency were investigated, with characterizations pre- and post-desulfurization. The experimental results showed that Cu significantly improved desulfurization performance of calcium-based materials, shifting the mechanism from adsorption- to catalysis-dominated. Among factors, temperature notably influenced calcium-based materials. Reaction kinetics analyses revealed that temperature boosted both rates of the surface chemical reaction-controlled stage and the internal diffusion process, explaining its significant impact. DFT simulations indicated Ca(OH)2(101) surfaces strongly adsorbed reactants but suffered from saturation and competitive adsorption, hindering efficiency. In contrast, Cu-modified surfaces, while exhibiting reduced adsorptive capacity, introduced new catalytic sites with low energy barriers, thereby enhancing desulfurization efficiency, particularly in the presence of water, which can directly participate in the subsequent formation of sulfate ions.

Nitrogen-doped hierarchically porous carbon derived from biomass for efficient capture of iodine

Effectively capturing radioactive iodine formed during nuclear energy utilization is crucial for environment protection and human health. Herein, we present a facile and effective strategy to synthesize a nitrogen-doped hierarchically porous carbon from corn stalks for iodine capture in gaseous and liquid environment. The as-prepared carbon material possesses a hierarchically micro/mesopore structure with highly developed mesopores, a large specific surface area (1892.99 m2 g−1), and suitable nitrogen-doping (2.32 at.%). As a result, the carbon material exhibits efficient iodine capture performance, showing a large capture capacity of 3.14 g g−1 at 80 °C for iodine vapor and a fast removal rate of ∼97 % within 5 min for iodine aqueous solution, outperforming most of carbon-based materials for iodine capture reported in literatures. Thermodynamic and kinetic analyses reveal that the adsorption of iodine vapor conforms to the Langmuir model and pseudo-second-order model, respectively. Density functional theory calculation results indicate that the doped-nitrogen heteroatoms strengthen the interaction between iodine molecules and carbon, which can enhance the affinity for iodine molecules by forming a donor–acceptor complex through the donation of lone-pair electrons, thereby facilitating effective capture of iodine. This work develops a biomass-based porous carbon with potential to efficiently capture radioactive iodine.

Influence of alumina on the performance of Ag/ZnO based catalysts for carbon dioxide hydrogenation

We study silver-zinc oxide type catalysts with and without the addition of alumina and perform structural analysis and activity tests for the hydrogenation of carbon dioxide. Adding alumina has a dispersing effect on the zinc oxide without structurally altering the silver phase. An alumina-surface enriched ZnO/Al2O3 phase is observed with an increased surface reducibility. Ag/ZnO has a high selectivity towards carbon monoxide (63 ± 12 %) and methane (24 ± 3 %) and low selectivity towards methanol (13 ± 0.5 %). Operando infrared (SSITKA-FTIR) and mass spectrometric product detection indicate methane formation via an adsorbed carbon monoxide (COads) intermediate. The selectivity changes gradually with increasing alumina content, up to 80 ± 3 % toward methanol, and 20 ± 4 % carbon monoxide without methane detection, combined with a tripling of the space time yield to 0.65 ± 0.02mmolMeOH*gcat-1*h−1 at 250 °C and 30 bar. Kinetic analysis suggests that the selectivity change originates from hindering the CO-pathway, while the formate pathway leading to methanol remains active.

Directly Regenerating of Spent LiCoO2 with Gradient F-Doped Subsurface towards Ultra-Stable Storage Properties.

As great potential recycling strategy, the direct regeneration of spent LiCoO2 (LCO) is beneficial for lowering environmental pollutions and promoting global sustainability. However, owing to the using of binder and electrolyte, some fluorine impurities would be remained into spent materials. Considering the doping behaviors of F-elements, their suitable content introducing would facilitate the energy-storage abilities of regenerated LCO. Herein, through the tailored introduction of F-elements, spent LCO are successfully regenerated with physical-chemical evolutions. Benefitting from the existed oxygen vacancies, the diffusion energy-barrier of F-elements is reduced from 1.73 eV to 0.61 eV, facilitating the establishment of gradient F-doped subsurface, along with the formation of rigid CoO5F. Meanwhile, excess F-elements (1 wt %, as a threshold) lead to the formation of LiF passivation layer on the surface. Thus, the as-optimized sample displays a considerable capacity of 154.4 mAh g-1 even at 5.0 C, with retention rate (88.3 %) in 3.0-4.5 V. Supported by detailed electrochemical and kinetic analysis, the structural advantages are confirmed to boost the improved redox activity of Co-ions and the alleviating of irreversible oxygen-release. Give this, the work is anticipated to reveal the evolutions of regenerated LCO with the introduced F-elements, whilst providing the practical regeneration strategies toward excellent high-voltage properties.

Optimizing solvothermal synthesis of aluminum-loaded pomelo peel for enhanced fluoride adsorption using response surface methodology

Addressing fluoride contamination in water remains a critical challenge, particularly when seeking cost-effective solutions. In this study, we present a novel aluminum-loaded pomelo peel adsorbent (PPA-Al), synthesized via a one-step solvothermal method, with parameters optimized through response surface methodology. The ideal conditions were established at 2.96 g of pomelo peel, 2 g of NaAlO₂, a reaction temperature of 168.62 °C, and a duration of 140.78 minutes. Characterization of PPA-Al revealed an abundance of hydroxyl and carbon functional groups, enhancing its reactivity with coordination unsaturated aluminum sites. Adsorption isotherms conformed to both Langmuir and Freundlich models, achieving a maximum adsorption capacity of 88.81 mg·g⁻¹, outperforming many existing biomass-based adsorbents. Kinetic analysis indicated that fluoride adsorption followed a pseudo-second-order model, with thermodynamic evaluations suggesting that this process is a spontaneous endothermic reaction. Characterization techniques, including Fourier transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS), confirmed interactions between fluoride ions and -OH and -CH groups, as well as the formation of Al-F bonds. Notably, the use of PPA-Al reduces groundwater treatment costs by approximately 74.33∼83.24 % compared to activated alumina, and it can be reused multiple times without reagent regeneration. These findings highlight PPA-Al as a promising, efficient material for fluoride removal, while also demonstrating innovative applications for agricultural waste.

Soot formation and laminar combustion characteristics of anisole: ReaxFF MD simulation and kinetic analysis

The application of biomass energy is one of the important ways to achieve carbon neutrality and deal with global warming. The study on the combustion mechanism of anisole, an oxygen-containing fuel, is helpful for biofuel large-scale application. In this study, the soot formation and laminar combustion characteristics of anisole were analyzed by reactive force field molecular dynamics (ReaxFF MD) and kinetic simulation, respectively. ReaxFF MD simulation studies had shown that soot formation of anisole combustion occurred in three stages, stage 1 (0–1 ns), stage 2 (1–2.5 ns), stage 3 (2.5–6 ns). The three stages represented the pyrolysis of the fuel, the developmental stage of the soot, and the graphitization stage of the soot, respectively. During the combustion of anisole, primary mechanisms for the soot formation were as follows: H-abstraction-C2H2-addition, carbon-addition-hydrogen-addition, internal ring formation and long carbon chain link. The formation of soot graphitization exhibited different morphologically behaviors: from flakes to onions to spheres with fewer branched chains. From the study of the laminar combustion characteristics of anisole, it can be found that the laminar burning velocities increased along with the increase of temperature, while the opposite trend was shown along with the increase of pressure. The sensitivity coefficient of naphthalene, the main soot precursor, revealed that the main promotional reactions for soot formation were R5 (O2 + H < = > O + OH), R36 (CO + OH < = > CO2 + H).

Axial Coordination Engineering on Fe-N-C Materials for Oxygen Reduction: Insights from Theory.

Axial coordination engineering has emerged as an effective strategy to regulate the catalytic performance of metal-N-C materials for oxygen reduction reaction (ORR). However, the ORR mechanism and activity changes of their active centers modified by axial ligands are still unclear. Here, a comprehensive investigation of the ORR on a series of FeN4-L moieties (L stands for an axial ligand) is performed using advanced density functional theory (DFT) calculations. The axial ligand has a substantial effect on the electronic structure and catalytic activity of the FeN4 center. Specially, FeN4-C6H5 is screened as a promising active moiety with superior ORR activity, as further revealed by constant-potential calculations and kinetic analysis. The enhanced activity is attributed to the weakened *OH adsorption caused by the altered electronic structure. Moreover, microkinetic modeling shows that at pH=1, FeN4-C6H5 possesses an impressive theoretical half-wave potential of ~1.01 V, superior to the pristine Fe-N-C catalysts (~0.88 V) calculated at the same level. These findings advance the understanding of the ORR mechanism of FeN4-L and provide guidance for optimizing the ORR performance of single-metal-atom catalysts.

Directly Regenerating of Spent LiCoO2 with Gradient F‐Doped Subsurface towards Ultra‐Stable Storage Properties

AbstractAs great potential recycling strategy, the direct regeneration of spent LiCoO2 (LCO) is beneficial for lowering environmental pollutions and promoting global sustainability. However, owing to the using of binder and electrolyte, some fluorine impurities would be remained into spent materials. Considering the doping behaviors of F‐elements, their suitable content introducing would facilitate the energy‐storage abilities of regenerated LCO. Herein, through the tailored introduction of F‐elements, spent LCO are successfully regenerated with physical‐chemical evolutions. Benefitting from the existed oxygen vacancies, the diffusion energy‐barrier of F‐elements is reduced from 1.73 eV to 0.61 eV, facilitating the establishment of gradient F‐doped subsurface, along with the formation of rigid CoO5F. Meanwhile, excess F‐elements (1 wt %, as a threshold) lead to the formation of LiF passivation layer on the surface. Thus, the as‐optimized sample displays a considerable capacity of 154.4 mAh g−1 even at 5.0 C, with retention rate (88.3 %) in 3.0–4.5 V. Supported by detailed electrochemical and kinetic analysis, the structural advantages are confirmed to boost the improved redox activity of Co‐ions and the alleviating of irreversible oxygen‐release. Give this, the work is anticipated to reveal the evolutions of regenerated LCO with the introduced F‐elements, whilst providing the practical regeneration strategies toward excellent high‐voltage properties.

Modulatory Effect of Nicotinamide Adenine Dinucleotide Phosphate (NADPH) on the 2-Oxoglutarate Mitochondrial Carrier

The 2-oxoglutarate carrier (OGC), pivotal in cellular metabolism, facilitates the exchange of key metabolites between mitochondria and cytosol. This study explores the influence of NADPH on OGC transport activity using proteoliposomes. Experimental data revealed the ability of NADPH to modulate the OGC activity, with a significant increase of 60% at 0.010 mM. Kinetic analysis showed increased Vmax and a reduction in Km for 2-oxoglutarate, suggesting a direct regulatory role. Molecular docking pointed to a specific interaction between NADPH and cytosolic loops of OGC, involving key residues such as K206 and K122. This modulation was unique in mammalian OGC, as no similar effect was observed in a plant OGC structurally/functionally related mitochondrial carrier. These findings propose OGC as a responsive sensor for the mitochondrial redox state, coordinating with the malate/aspartate and isocitrate/oxoglutarate shuttles to maintain redox balance. The results underscore the potential role of OGC in redox homeostasis and its broader implications in cellular metabolism and oxidative stress responses.

Paradendryphiella arenariae an endophytic fungus of Centella asiatica inhibits the bacterial pathogens of fish and shellfish

IntroductionAquaculture has been considered a major food-producing sector in the world during the last few decades. The foremost constraint in the development of aquaculture is bacterial disease control and management. Since various fish pathogens are resistant to conservative treatments, it is essential to screen new and effective alternative antibacterial agents. Endophytic fungi are microorganisms that live in the plant’s internal tissues without harming its host. Endophytic fungi have proven themselves as reliable sources of novel bioactive compounds that can be used as antibacterial agents.MethodsIn the present study, fifteen morphologically different endophytic fungi were isolated from the fresh and healthy stem section of Centella asiatica. The active endophytic fungal crude extracts were tested for agar well diffusion assay, Minimum Inhibitory Concentration, Minimum Bactericidal Concentration assays, Time-kill kinetic analysis, Brine shrimp lethality assay.Results and DiscussionAgar plug diffusion and agar well diffusion assays revealed that endophytic fungus CAS1 exhibits maximum antagonistic activity against bacterial fish pathogens. The ethyl acetate crude extract of CAS1 exhibited the maximum zone of inhibitions against Aeromonas hydrophila (21 ± 0.11 mm), Aeromonas caviae (18 ± 0.1 mm), Edwardsiella tarda (23 ± 0.11 mm), Vibrio anguillarum (19 ± 0.05 mm) and Vibrio harveyi (20 ± 0.27 mm). The MIC and MBC values extract varied reliant on the trial pathogens ranging between 12.5-100 μg/mL and 25-100μg/mL correspondingly. The morphological and molecular characterization of potential isolate CAS1 was confirmed as Paradendryphiella arenariae by 18S rRNA ITS gene sequencing with 99.18% identity. This is the foremost findings to study the antagonistic effect of Paradendryphiella arenariae isolated from the stem of Centella asiatica against bacterial fish pathogens which can be used as natural effective antibacterial agents in aquaculture.

Assessment on the rings cleavage mechanism of polycyclic aromatic hydrocarbons in supercritical water: A ReaxFF molecular dynamics study

The ring opening reaction of polycyclic aromatic hydrocarbons (PAHs) is one of the rate-determining steps of coal gasification within supercritical water, while the involvement of water remains debated between addition and hydrogen abstraction theories. In this study, reactive molecular dynamics (MD) simulations were performed to explore the ring opening reaction of naphthalene, the simplest PAH, at temperatures of 2500–2700 K. Species and elementary reactions were quantitatively extracted from bond order trajectories using an in-house program. Reaction path analysis shows that ring opening paths of naphthalene mainly include thermolysis, H˙ atom abstraction, and O˙H radical addition. Flux analysis indicates O˙H radical largely from the H˙ + ▪ = O˙H + ▪ reaction, while the water dissociation ▪ = O˙H + H˙ reaction is near equilibrium, seldom contributing to the O˙H source. This atomistic kinetics analysis is intended to help the modeling of supercritical water gasification of coal.

Fabrication of MOF-carbonized materials as ozone catalysts for water purification: Exploration of catalytic mechanisms

Due to their stability, the advent of MOF-derived catalysts ensures the applications of MOF-based materials, and they have been used in many catalytic systems. However, their application in ozone catalysis is limited, and the relevant mechanisms remain unclear. In this study, three MOF-carbonized materials (Me-MOF-C, Me: Fe, Cu, and Zn) were synthesized as ozone catalysts for water purification. When combined with ozone, all the Me-MOF-C promoted target removal compared to the Me-MOF/O3 systems, and Fe-MOF-C was optimal due to its superior ozone utilization efficiency. ROS quantification indicated that the •OH in the Fe-MOF-C/O3 system was approximately 48.0 times that of the single ozone system. Kinetic analysis demonstrated that over 90 % of the target was removed by •OH in the Fe-MOF-C/O3 system. Then, the catalytic mechanism of Fe-MOF-C was investigated. The surface hydroxyl and protonation effect of Fe-MOF-C presented limited activity. In comparison, H2O2 in the solution and Fe2+ on the Fe-MOF-C surface were more crucial. AcOH control experiments revealed a positive correlation between •OH and H2O2. H2O2 decomposition experiments further indicated that although the Fe-MOF-C/O3 system utilized H2O2 faster, only the coexistence of ozone and Fe-MOF-C could facilitate it more. Results of Fe2+ regulation suggested that it was related to the way of surface Fe2+ acted. The main contribution of Fe2+ to producing •OH was not through Fe2+ ozonation or reaction with H2O2, but it likely involved the efficient conversion of H2O2 into HO2ˉ, which subsequently decomposed ozone to produce •OH more efficiently. Finally, a possible catalytic pathway was proposed.

Kinetic Analysis and Metabolism of Poly(Adenosine Diphosphate-Ribose) Polymerase-1-Targeted 18F-Fluorthanatrace PET in Breast Cancer.

The poly(adenosine diphosphate-ribose) polymerase inhibitors (PARPi) have demonstrated efficacy in ovarian, breast, and prostate cancers, but current biomarkers do not consistently predict clinical benefit. 18F-fluorthanatrace (18F-FTT) is an analog to rucaparib, a clinically approved PARPi, and is a candidate biomarker for PARPi response. This study intends to characterize 18F-FTT pharmacokinetics in breast cancer and optimize image timing for clinical trials. A secondary aim is to determine whether 18F-FTT uptake in breast cancer correlates with matched frozen surgical specimens as a reference standard for PARP-1 protein. Methods: Thirty prospectively enrolled women with a new diagnosis of breast cancer were injected with 18F-FTT and imaged dynamically 0-60 min after injection over the chest, with an optional static scan over multiple bed positions starting around 70 min. Kinetic analysis of lesion uptake was performed using blood-pool activity with population radiometabolite corrections. Normal breast and normal muscle reference tissue models were compared with PARP-1 protein expression in 10 patients with available tissue. Plasma radiometabolite concentrations and uptake in tumor and normal muscle were investigated in mouse xenografts. Results: Pharmacokinetics of 18F-FTT were well fit by Logan plot reference region models of reversible binding. However, fits of 2-tissue compartment models assuming negligible metabolite uptake were unstable. Rapid metabolism of 18F-FTT was demonstrated in mice, and similar uptake of radiometabolites was found in tumor xenografts and normal muscle. Tumor 18F-FTT distribution volume ratios relative to normal muscle reference tissue correlated with tissue PARP-1 expression (P < 0.02, n = 10). The tumor-to-normal muscle ratio from a 5-min frame between 50 and 60 min after injection, a potential static scan protocol, closely corresponded to the distribution volume ratio relative to normal muscle and correlated to PARP-1 expression (P < 0.02, n = 10). Conclusion: This study of PARPi analog 18F-FTT showed that uptake kinetics invivo corresponded to expression of PARP-1 and that 18F-FTT quantitation is influenced by radiometabolites that are increasingly present late after injection. Radiometabolites can be controlled by using optimal image acquisition timing or normal muscle reference tissue modeling in dynamic imaging or a tumor-to-normal muscle ratio. Optimal image timing for tumor-to-normal muscle quantification in humans appears to be between 50 and 60 min after injection. Therefore, a clinically practical static imaging protocol commencing 45-55 min after injection may sufficiently balance 18F-FTT uptake with background clearance and radiometabolite interference for quantitative interpretation of PARP-1 expression invivo.

Advancements in renewable energy: Achieving milder reaction conditions in biodiesel synthesis from green seed canola oil with pristine ZIF-8

This study explores the enhancement of reaction conditions for biodiesel production from green seed canola oil through the methanolysis reaction using pristine ZIF-8 as a catalyst. The research focused on the effects of varying the methanol-to-oil molar ratio, temperature, and reaction time as well as their combined effects on biodiesel yield. To analyze these factors, Response Surface Methodology (RSM) paired with Central Composite Design (CCD) was employed. The quadratic model was identified as the best fit for the experimental data, evidenced by a high determination coefficient (R²) of 0.99, with all model parameters proving significant. A comprehensive characterization of the catalyst was conducted using various techniques. The surface and pore properties were examined using N2 adsorption-desorption measurements. X-ray diffractometry (XRD) was employed for structural analysis, while X-ray photoelectron spectroscopy (XPS) presented the binding information of the sample. Fourier transform infrared spectroscopy (FT-IR) provided insights into the functional groups present. Thermal gravimetric analysis (TGA) assessed the thermal stability of the catalyst. Results revealed that ZIF-8 significantly improved biodiesel production, with optimal conditions identified at a reaction temperature of 160 °C, a duration of 2.5 h, and methanol to oil (M/O) molar ratio of 26, resulting in a biodiesel yield of 85 %. This yield is close to the predicted value, demonstrating the reliability of the developed model. The kinetic analysis was performed under optimal reaction conditions. From this, the activation energy (Ea) and the pre-exponential factor (A) were obtained to be 14.5 kJ/mol and 3.4×10−7 min−1, respectively. The use of pristine ZIF-8 in biodiesel production from a low-quality and cheap oil offers a more efficient and milder reaction conditions, with potential for significant reductions in production costs and environmental impact.