This research is to assess the influences of the inertial mass from the girder on the dynamic characteristic, dynamic response, and structure-soil interaction of a pile-soil-pier subsystem in a scale-model of a cable-stayed bridge. Therefore, both connection configurations between the pile-soil-pier and girder, including the sliding and fixed connections, were designed to present various inertial mass from the superstructure delivered to the pile-soil-pier. The pile-soil-pier supported by a 3 × 3 pile-group in mixed soil placed in a shear box was tested using shaking tables. The dynamic characteristics, seismic responses, inertial interactions, and pile group effects of the pile-soil-pier between the sliding and fixed connections were analyzed under three input motions with different shaking amplitudes. These results showed that more inertial mass from the girder significantly increased the reinforcement strain and bending moment at the column bottom and pile top, displacement at the column top, inertial interaction effects, and pile group effects of the pile-soil-pier due to the sliding connection changing to the fixed connection. The inertial mass increment from the girder noticeably decreased the peak accelerations of the column of the pile-soil-pier when subjected to three input motions with different amplitudes. However, the inertial mass insignificantly affected the accelerations of the pile and free-soil. Therefore, the corresponding kinematic interaction effects were almost unaffected by the inertial mass. Additionally, the evident pile group effects were observed in the sliding and fixed connections between the pile-soil-pier and girder. The numerical model could approximately reproduce the macroscopic seismic responses of the pile-soil-piers with sliding and fixed connections and capture the typical response variations induced by the connection configuration change.