Forty percent of rivers, 45% of lakes and 50% of estuaries assessed by the National Water Quality Inventory (2000) were not clean enough to support designate d fishing and swimming uses. Pathogens were found to be one of the leading causes of impairments in these waters. Urban runoff is recognized as a leading source of organisms potentially indicating the presence of pathogens. Urban runoff can be defined as any discharge from a separate storm drainage system. Urban runoff traditionally had been defined to in clude precipitation and wash off from lawns and other landscaped areas, buildings, roadways and parking lots. However, other flows may enter the storm drainage system from such sources a s infiltrating groundwater, leaking domestic water supplies and sewage, washwaters, and other inappropriate entries to the storm drainage system. This research was conducted to quantify th e levels of indicator bacteria, and their sources, in urban areas. The main objective of t his research was to identify possible sources of E. coli and enterococci bacteria in dry and wet-weather flows in storm drainage systems. An urban area consists of many different kinds of land uses such as residential, institutional, commercial, industrial open spaces, etc. Each type of la nd use consists of various types of source areas, such as roofs, parking lots, landscaped areas, play grounds, driveways, undeveloped areas, sidewalks. Four representative source area types were sa mpled during this research; including rooftops, parking lots, open spaces, and streets. Two paralle l sites were sampled for each source area type; one affected by birds and other animals, and an other set with less influence from birds and other animals. A section of Cribbs Mill Creek in Tusc aloosa, Alabama, was also selected for dry weather sampling at outfalls. The section of the cr eek was selected such that the drainage areas contributing to outfalls had either commercial or residential land uses. Potential inappropriate discharge water samples were also obtained, including influent samples from the Tuscaloosa sewage treatment plant, local springs, irrigati on runoff water, domestic water taps, car wash, and laundry water. Overall, total coliforms, E. coli and enterococci bacterial analyses were conducted on 202 wet weather and 278 dry weather flow water samples. All samples were analyzed using IDEXX Quantitray enumeration procedures. E. coli and enterococci levels larger than 2,400 and 24,000 MPN/100 mL, r espectively, were observed in wet weather samples collected from various source areas which could not possibly be contaminated with sanitary sewage. The levels of ind icator bacteria present in the urban runoff source area samples exceeded the EPA 1986 single sa mple maximum value water quality criteria in 31% of the samples for E. coli and in 74% of the samples for enterococci. The geometric mean criteria were exceeded in 100% of the source area samples. Since both the indicator organisms studied ( E. coli. and enterococci) only originate in intestines of warm