Kilogram Of Dry Matter Research Articles

Effect of an algae feed additive on reducing enteric methane emissions from cattle.

Alga 1.0, a product containing bromoform, was fed to cattle to evaluate its effects on methane (CH 4 ) and carbon dioxide (CO 2 ) emissions and diet digestibility. Twelve nonlactating, nonpregnant Jersey cows (490 ± 19kg body weight) were used in four replicated 3 × 3 Latin squares with three periods, each consisting of 21d. Cows were blocked by feed intake (averaged intakes over 4wk prior to trial) and assigned randomly to one of three treatments. Treatments included Alga 1.0 fed at 0, 69, and 103g/d in a 0.454kg/d dry matter (DM) top-dress daily in a modified distillers grains plus solubles (MDGS) carrier. Diet consisted of 60% dry-rolled corn, 20% corn silage, 15% modified distillers grains, and 5% supplement (DM basis). Headbox-style indirect calorimeters were utilized to evaluate gas production from individual cows with two nonconsecutive 23-h collections in each period. Data were analyzed using the GLIMMIX procedure of SAS with cow within square as experimental unit and as a random effect, and treatment and period as fixed effects. Linear and quadratic contrasts were used to compare treatments. Feeding Alga 1.0 linearly reduced dry matter intake (DMI, P < 0.01) by 10.1% for 69g/d inclusion and 13.3% for 103g/d inclusion compared to the control. Nutrient intakes decreased linearly (P < 0.01) due to lower DMI, but nutrient digestibility was not impacted (P ≥ 0.28). Inclusion of Alga 1.0 did not impact gross energy or digestible energy concentration of the diets expressed as Mcal/kg DM (P ≥ 0.22) but did linearly reduce energy intake (Mcal/d; P < 0.01). Feeding Alga 1.0 linearly reduced enteric CH4 emissions measured as g/kg DMI (P < 0.01) by 39 and 64% for 69g/d and 103g/d inclusion, respectively. Linear reductions (P < 0.01) of 64% to 65% were also observed in enteric CH4 emissions when expressed per kilogram of DM or organic matter digested. Respired CO2 as g/d linearly decreased (P = 0.03) for cattle fed Alga 1.0 but did not differ when expressed as g/kg of DMI (P ≥ 0.23). Oxygen consumption did not differ between treatments for g/d and g/kg DMI (P ≥ 0.19). In conclusion, feeding Alga 1.0 reduced DMI up to 13.3%, did not impact digestibility, and significantly reduced CH4 emissions up to 63%.

Assessment of the inclusion of a feed additive of sodium humate derived from freshwater sapropel in diets for broiler chickens.

Poultry production is the fastest growing livestock industry in the world, as the rapid growth of and efficient absorption of feed by poultry ensure the production of poultry meat with a relatively low carbon footprint. Seeking new ways to increase livestock productivity as well as poultry product quality, the number of research studies on the use of humic substances of various origins in livestock farming has increased significantly, emphasizing the role of feed additives derived from local resources. The unique capability of humic substances to improve metabolic processes allows the immune protection of the bird body to be strengthened and production efficiency to be increased. This study aimed to identify the effects of sodium humate (NaHum) on the growth performance of broiler chickens and selected blood and ileum microbiota parameters. Dietary research was conducted 2 times under production conditions in a poultry facility of a commercial company, with 210 1-day-old, unsorted broiler chickens of both sexes (Ross 308). The broiler chickens were fed with standard commercial feed, the rearing period of 35 days, and slaughtered on day 36. Sodium humate additive was added to drinking water for the research groups of broilers in period from 8th to 35th day of life, 25 mL (Group 1, n = 2 × 35) and 50 mL (Group 2, n = 2 × 35) per liter of drinking water. Sodium humate contained an average of 4.48% dry matter, a kilogram of dry matter containing 104.3 g of crude protein, 3.6 g of crude fiber and 0.9 g of crude fat, 14.3 MJ of metabolic energy, and 5.8 MJ of energy for live weight gain, as well as a very high content of crude ash -759.8 g, including 4.2 g Ca, 4.2 g Na, and 4.81 g Fe, the dry matter digestibility of NaHum was 87.0%, and the absorption capacity of dry matter was 113.2%., the pH level was 13.0, i.e., alkaline. At the end of the dietary research, the productivity and economic efficiency of the research groups of broilers were calculated by live weight gain, carcass weight, feed conversation ratio, and blood and intestinal samples of broilers were analyzed to identify the effects of NaHum on the growth performance and health status of broilers. Dietary research found that adding 25 mL/L and 50 mL/L of NaHum to drinking water for the broiler chickens increased their live weights at the selling age, average live weight gains by 3.06-3.93%, and carcass weights by 5.07-6.06%, while feed conversion increased in terms of both live weight (1.5 and 1.51) and carcass weight (1.84 and 1.86) compared with the control group. The best economic performance in terms of the economic efficiency index and the cost index (CI) was found in Group 1, which was fed with the NaHum additive at an intake rate of 25 mL/L. The NaHum additive modulated the ileal microbiota and metabolic processes in the broiler body. At the same time, a significant decrease in the levels of total protein, alkaline phosphatase and phosphorus (P) in blood was found in the research groups. Considering the positive effects of NaHum derived from freshwater sapropel on the productivity and economic efficiency of broiler chickens, the NaHum feed additive should be further investigated on a larger scale to obtain results that could reasonably be used in practice. This study concluded that a decrease in P levels in the blood was observed when NaHum was added to the drinking water; therefore, it is important to continue the research to draw reasonable conclusions on the effects of NaHum in liquid form on the health performance of farm animals.

Dietary Neutral Detergent Fiber Levels Impacting Dairy Cows' Feeding Behavior, Rumen Fermentation, and Production Performance during the Period of Peak-Lactation.

This study investigated the impact of dietary neutral detergent fiber (NDF) levels (25.49%, 28.65%, 31.66%, and 34.65%, respectively) on the feeding behavior, rumen fermentation, cellulolytic bacteria, and production performance of dairy cows during peak lactation. A feeding experiment was conducted using four fistulated Holstein dairy cows (600 ± 25 kg) with days in milk (50 ± 15 days), employing a 4 × 4 Latin square design to assign the cows to four groups. The results demonstrated that increasing NDF levels in the diet had the following effects: (1) A linear decrease in dry matter intake (DMI), NDF intake, and physically effective NDF8.0 (peNDF8.0) intake; a linear increase in the average time spent eating and ruminating, as well as the time spent eating and ruminating per kilogram of dry matter (DM); a quadratic response in the time spent ruminating per kilogram of NDF and peNDF8.0. (2) A linear increase in average pH value, acetate concentration, and the proportions of Fibrobacter succinogenes and Ruminococcus flavefaciens among total bacteria; a linear decrease in ammonia nitrogen (NH3-N) concentration, microbial crude protein (MCP), total volatile fatty acid (TVFA), propionate, butyrate, and lactate. (3) A linear decrease in milk yield, milk protein percentage, and nitrogen efficiency of dairy cows; a linear increase in milk fat percentage and milk urea nitrogen (MUN) concentration. Based on the combined results, it was found that diets with 25% and 34% NDF had detrimental effects on the feeding behavior, rumen fermentation, and production performance of dairy cows. However, the diet with 28% NDF showed superior outcomes in production performance compared to the one with 31% NDF. Therefore, it is strongly recommended to include a diet containing 28% NDF during the critical peak lactation period for dairy cows.

Effects of Acacia mearnsii added to silages differing in nutrient composition and condensed tannins on ruminal and manure-derived methane emissions of dairy cows

This study investigated the effects of acacia (extract of Acacia mearnsii) and sainfoin (Onobrychis viciifolia) as condensed tannin (CT)-rich sources on ruminal and manure methane (CH4) emissions in comparison with non-CT silages characterized by different contents of the cell wall and water-soluble carbohydrates. In a 3 × 6 incomplete Latin square design, 30 Holstein cows (63 ± 23 d in milk; mean ± SD; 33.8 ± 7.6 kg of milk per day, body weight 642 ± 81 kg) were provided with ad libitum access to 1 of 6 total mixed rations comprising 790 g of silage and 210 g of concentrate per kilogram of dry matter (DM). The silages were either rich in sainfoin [neutral detergent fiber (NDF): 349 g/kg of DM], perennial ryegrass (NDF: 420 g/kg of DM), or red clover (NDF: 357 g/kg of DM). Each silage was supplemented with 20 g/kg (of total diet DM) of acacia or straw meal. Feed intake and milk yield were recorded daily. Milk composition and ruminal fluid characteristics and microbiota were analyzed. The individual ruminal CH4 production was determined using the GreenFeed system, and CH4 emissions from the manure of cows fed the same diets were measured in a parallel experiment over 30 d at 25°C using a dynamic flux chamber. The CT sources did not reduce CH4 yield or emission intensity. Acacia reduced milk production (from 26.3 to 23.2 kg/d) and DM intake (from 19.7 to 16.7 kg/d) when supplemented with ryegrass, and both CT sources reduced the milk protein content and yield. Acacia supplementation and ryegrass silage reduced the ruminal acetate:propionate ratio. Furthermore, during acacia treatment, the abundance of Methanobrevibacter archaea tended to be lower and that of Thermoplasmata was higher. Acacia reduced the CH4 emissions from manure for the ryegrass group by 17% but not for the sainfoin and clover groups. Feeding sainfoin silage resulted in the lowest manure-derived CH4 emissions (-47% compared with ryegrass). In conclusion, acacia reduced ruminal CH4 production by 10%, but not emission intensity, and the mitigation effect of sainfoin depended on the silage to which it was compared. Because mitigation was partially associated with animal productivity losses, careful evaluation is required before the implementation of tanniferous feeds in farm practice.

Effects of dietary Sanguisorba minor, Plantago lanceolata, and Lotus corniculatus on urinary N excretion of dairy cows

Context Mitigating urinary nitrogen (N) losses is an important target of sustainable cattle nutrition concepts. One option to achieve this may be dietary inclusion of tanniferous herbs. Aims Aim of the study was to investigate herbs with different profiles of tannins for their efficiency to abate urinary N losses. Small burnet (Sanguisorba minor) with high concentrations of total tannins, plantain (Plantago lanceolata) with low concentrations and birdsfoot trefoil (Lotus corniculatus) with expectedly high concentrations of condensed tannins were included in the treatments. Methods The test plants were mixed in dried form into a grass–maize-silage diet at 80 g/kg of dietary dry matter. They replaced dried perennial ryegrass (control). Twenty-four multiparous dairy cows were randomly allocated to the four diets. Intake, eating time, rumination time, and milk yield were recorded individually, and representative samples of milk and excreta were collected and analysed six times within 14 days, following 10 days of adaptation. The diets with ryegrass, birdsfoot trefoil, plantain or burnet contained, per kilogram of dry matter, 0, 1.8, 1.2 and 1.9 g condensed tannins, 0.1, 1.9, 1.7 and 15.5 g total tannins, and 26.2, 28.5, 27.5 and 26.6 g N. Key results Milk yield and composition were not affected by treatment, apart from a decline in milk protein content when feeding plantain. Milk urea concentration was reduced with burnet by more than 30%, compared with the control and plantain. Birdsfoot trefoil also reduced milk urea concentration, but to a lesser degree. Furthermore, the burnet treatment substantially shifted N excretion from urine to faeces (about 30% lower urine N losses). All treatments lowered the proportion of fine particles of &lt;1.0 mm in faeces, what might be due to high fibre content of the control. Conclusions At dietary proportion of 80 g/kg, burnet is a forage herb with potential to reduce ruminal ammonia generation as indicated by reduced urinary N and milk urea. Plantain and birdsfoot trefoil had no or negligible effects. Implications The study indicated that small burnet could have potential as a feed additive for dairy cows in terms of N-use efficiency, lower emissions to the environment, and reduced animal metabolic stress.

Combined use of monensin and virginiamycin to improve rumen and liver health and performance of feedlot-finished steers.

Monensin and virginiamycin are included in beef cattle finishing diets as prophylaxis to minimize the incidence of ruminal acidosis and liver abscesses. Due to different and probably complementary modes of action, this study aimed to determine the effects of a combination of monensin and virginiamycin, both included in the diet at recommended doses, on ruminal health, the occurrence of liver abscesses, and growth performance of feedlot-finished cattle. One hundred and forty-four steers (6 animals/pen) were fed 1 of 3 corn-based finishing diets containing 30 mg of monensin (MN), 25 mg of virginiamycin (VM), or 30 and 25 mg of monensin and virginiamycin (MN + VM), respectively, per kilogram of dry matter. Ruminal pH probes were inserted into two animals per pen and set to record pH every 10 min. On d 100, animals were slaughtered, and rumens and livers were recovered, on which occurrence and degree of ruminal damage, prevalence and number of liver abscesses, and liver scores (A-: livers with no more than two small abscesses; A+: livers with at least one large abscess or more than four medium abscesses; A: any other abscessed liver) were determined. Simultaneous inclusion of monensin and virginiamycin resulted in a 4.3% decrease (P < 0.04) in dry matter intake (DMI; 8.8, 9.2, and 9.2 ± 0.19 kg/d for MN + VM, MN, and VM-fed animals, respectively) and similar (P > 0.13) average daily body weight gain (ADG; 1.49 ± 0.021 kg/d) and hot carcass weight (HCW; 269 ± 1.7 kg), compared with feeding diets containing one additive or the other. Therefore, in terms of ADG, a 9.4% improvement (P < 0.01) in feed efficiency was observed in MN + VM-fed animals. Backfat thickness (5.6 ± 0.08 mm) and ribeye area (69.9 ± 0.53 cm2) remained unaffected (P ≥ 0.74), as well as the minimum (4.98 ± 0.047), mean (6.11 ± 0.037), and maximum ruminal pH (7.23 ± 0.033) values and the time (125 ± 22.3 min/d), area (57.67 ± 12.383 pH × h), and episodes (22 ± 3.8 bouts) of pH below 5.6 (P ≥ 0.12). Overall, prevalence (24 ± 3.4%) and the number of liver abscesses (1.6 ± 0.14 abscesses/abscessed liver), liver scores (20 ± 3.1% of A- and 4 ± 1.8% of A livers), and prevalence (67 ± 3.5%) and degree of damage to the ruminal epithelium (2.5 ± 0.22% affected surface) were similar (P ≥ 0.18) across treatments; however, the occurrence of ruminal lesions tended (P ≤ 0.07) to be associated with that of liver abscesses and reduced ADG when feeding monensin alone.

The use of non-traditional legumes in the production of high-protein feed on irrigation

The article presents the results of research on the perennial legumes various types modern varieties attracting and adapting to the conditions of the Lower Volga region. The experiments were carried out on irrigated light chestnut soils. The completeness of the studied grasses seedlings was at the level of the traditional culture of purple hybrid alfalfa or slightly lower than its indicators. The creation of optimal growing conditions contributed to the formation of the maximum assimilation surface on the grasses sowing. In the second year of life, the leaf area by crops varied from 43 - 45 to 57 - 62 thousand m2/ha. The highest indices were in alfalfa and blue hybrid alfalfa and meadow clover. On these crops, the yield of green mass was formed at the level of 75 - 88, on the crops of birdsfoot trefoil and creeping crown vetch it was 62 - 65 t/ha. The qualitative indicators of the biomass of the studied legumes corresponded to the high requirements for the assessment of feed. The content of feed units in a kilogram of dry matter was 0.62 - 0.68, digestible protein - 120 - 171, and metabolizable energy - 9.7 - 10.3 MJ. Leguminous herbs improve soil fertility due to the nutrients contained in the accumulated root mass. After three years of use, from 142 to 240 kg of nitrogen, 60 to 90 kg of phosphorus and 80 to 145 kg of potassium enter the soil along with the organic matter.

Pollution from Transport: Detection of Tyre Particles in Environmental Samples

Transport is one of the most important sources of environmental pollution. More and more information has shown that one of the greatest sources of emissions from transport are emissions related to the release of microplastics from tyres. This is one of the most underestimated sources of emissions into the environment. In this study, environmental samples are analysed for the presence of these particles. For this purpose, optical methods and spectroscopic methods are used. Fourier transform infrared (FTIR) spectroscopy is used to identify synthetic rubber, most likely derived from car tyres. A complementary confocal microscopy method is used to confirm the FTIR results. The soil samples and road dust from the areas with heavy traffic are tested. An average of 372 ± 50 fragments per kilogram dry weight are detected in the soil samples. In the case of samples from the road, this number is 515 ± 20 fragments per kilogram of dry matter. In the samples, most of the microplastics come from tyres, which confirms the scientists’ assumptions about the amount of emissions in the environment. More than 90% of the black fragments later identified as tyre-derived synthetic rubber are found in the samples. A greater number of microplastics are found in road dust samples than in the soil. This may be due to the direct influence of braking, which causes greater accumulation of samples at the emission source than at a short distance into the soil. There is also a noticeable difference in the size of the fraction. In the case of soils, a fraction below 50 µm accounts for the majority of cases. When one analyses road dust samples, one may observe that most of the fractions are between 50 and 200 µm. This may be due to the possibility of smaller emission particles over longer distances and the greater degradation process that occurs in soils. The microplastics from the road dust are less degraded than the microplastics from the soil.

High-digestible silages allow low concentrate supply without affecting milk production or methane emissions

In this study, we tested a response function comprising responses in milk to changes in organic matter digestibility of silages and concentrate supply. We studied the effect of changes in silage digestibility and concentrate supply on milk yield, feed intake, body weight, and methane production using 60 Norwegian Red cows. The experiment was a complete randomized block design comprising 3 periods. The pre-experimental period lasted 20 d and all the cows were fed a common silage for ad libitum intake and concentrate according to yield. Next, response period 1 lasted 17 d and the cows were divided into 2 treatments, where a low-digestible silage (LDS) was fed to half of the cows, and the other half were fed a high-digestible silage (HDS). Both groups were fed silage for ad libitum silage intake. Concentrate was optimized according to the yield and type of silage offered. In this period, the effect of silage was evaluated using a mixed model, including the results from pre-experimental period, with parity as a covariate and animal as a random effect. In response period 2, which lasted 20 d, the concentrate level was evaluated by dividing the silage digestibility treatments further into 3 subgroups. Concentrate was increased by 2 kg of dry matter (DM) per day, decreased by 2 kg of DM/d, or remained unchanged. In response period 1, silage treatments were optimized to obtain similar yields and resulted in a lower concentrate offer to HDS treatment. However, the HDS treatment showed a 3.0 kg of DM/d higher total feed intake due to a higher than expected silage intake. This resulted in 3.5 kg higher energy-corrected milk (ECM). Methane emissions were similar between silage treatments, but HDS showed lower methane per kilogram of DM due to its higher intake. The effect of concentrate supply level and interaction with silage digestibility was evaluated using mixed models, including the results for response period 1, with parity as a covariate and animal as a random effect. The reduction in concentrate offer by 2 kg/d in response period 2 was compensated for by increased 1.3 kg of DM/d of silage intake for HDS, resulting in similar intake (22.1 kg of DM/d and 21.7 kg of DM/d without and with concentrate reduction, respectively) and ECM yields (29.4 and 29 kg of ECM without and with concentrate reduction, respectively). However, concentrate offer reduction could not be compensated for by increased silage intake for LDS and resulted in lower milk yields (27.5 kg of ECM). Increased concentrate showed a higher marginal ECM response (kg of ECM per kg of additional concentrate intake) for LDS (1.8 vs. 3.3 kg of ECM for HDS and LDS, respectively). Thus, the drop in milk yields could be compensated for by increased concentrate offers if LDS are fed. Total methane production increased with increased concentrate intake, regardless of silage digestibility. Methane emissions per unit of milk were affected by total DM intake rather than by changes in silage digestibility and concentrate level. The results of this study are based on short-term periods and could show differences if study periods were longer; the results should be interpreted accordingly.

Некоторые идеи по менеджменту и регулированию состояния коров в материнском стаде в предотельный период

Cow management and feeding during pre-and post-calving periods is one of the factors of the economic effi-ciency of animal husbandry. There are various methods of feeding lactating cows but at the same time this topic is understudied in Germany. The experiment is based on long-term data on the birth rate and weight of calves at the Eden agricultural enterprise (Saxony-Anhalt, Germany) which was chosen as the main one for the study. The av-erage live weight of calves obtained from first-calf cows was 52 ± 8 kg at birth. The data on the live weight of calves obtained from cows of the first, second, and third lactations are presented. The differences in the live weight of calves revealed immediately after birth. Estimative de-scription of the condition of lactating cows is given. The dependence of the feeding of cows on the quality of the feed material, the content of protein and dry matter is shown. On average, the diet consisted of 99% of forages and had dry matter content of 38 ±6%. The average ener-gy content was 9.8 MJ ME or 5.9 MJ NEL per kilogram of dry matter. Various diets for dry cows were also studied. During the experiment, only the proportions of straw in the diet were changed so that practical conclusions could be drawn. In total, 3 variants were used: TMR with 30% of straw [TMR 1], TMR with 60% of straw [TMR 2] and feed-ing with silage from pure grass after calving. Brief recom-mendations are proposed in the conclusion.

Research of feed protein obtaining technology

The introduction of high-grade mineral additives in feed allows to get balanced rations for livestock and poultry. It is known that the most effective use of them when feeding in the composition of feed. However, today, in real conditions, farms receive less than 30% of the required amount of chemical feed additives with compound feed. In addition, the outlined tendency towards a decrease in the production of compound feed due to the lack of grain and protein components indicates that the feed industry is not entirely able to solve this problem. Adequate feeding of dairy cows assumes an optimal intake of all essential nutrients in the body, which contributes to good health, normal reproduction and high productivity. A significant reduction in the energy deficit during this period can be achieved by introducing energy-rich feed concentrates, grass cutting and grass flour of high quality, root crops, etc. into the diet. The higher the milk yield of cows, the more energy should be in one kilogram of dry matter of the diet. This article proposes a technology for producing a protein-vitamin feed concentrate for full-value feeding of farm animals. To solve the fodder problem in animal husbandry, it is necessary to more widely use substandard raw materials, organic waste, ensuring their utilization within the framework of waste-free technology.

Total-tract digestibility and milk productivity of dairy cows as affected by trace mineral sources

The chemical characteristics associated with different sources of Cu, Zn, and Mn such as sulfate, hydroxychloride, or organic chelate may affect the interaction between the metals and other components present within the gut of a ruminant (i.e., microorganisms and nutrients). The present study aimed to evaluate the effect of different supplemental trace mineral strategies on apparent total-tract digestibility, rumen fermentation, and dairy productivity. Using 52 Holstein cows in a replicated 4 × 4 Latin square design with periods of 21 d, 4 treatments differing in their sources of Cu, Zn, and Mn were tested: sulfate form, hydroxychloride form, a mix of sulfate and organic chelate forms (70 and 30%, respectively), and a mix of hydroxychloride and organic chelate forms (70 and 30%, respectively). Treatments were formulated to provide 15, 40, and 20 mg of supplemental Cu, Zn, and Mn, respectively, per kilogram of dry matter. This level of supplementation, together with the basal level present in forages and feed ingredients, resulted in a total average supply of 19, 79, and 84 mg of Cu, Zn, and Mn, respectively, per kilogram of dry matter. Cows had ad libitum access to a total mixed ration, which provided 15.3% of crude protein, 21.7% of starch, and 35.3% of neutral detergent fiber (NDF). Data were summarized by period with trace minerals and period as fixed effects and the repeated cow as random effect using the MIXED procedure of SAS (SAS Institute Inc., Cary, NC). Apparent total-tract NDF and crude protein digestibility was reduced (-0.8% and -1.0%, respectively) when organic chelate trace minerals were fed, whereas apparent total-tract NDF digestibility was improved (+0.8%) when sulfate trace minerals were replaced by hydroxychloride trace minerals. Cows supplemented with the hydroxychloride source had lower ruminal butyric acid concentration compared with cows fed sulfate trace minerals (13.3 vs. 14.6%). In addition, fat- and protein-corrected milk and milk fat yields were improved (+1.0 kg/d and +51 g/d, respectively) in multiparous cows when trace minerals were supplemented as hydroxychloride compared with sulfate. These effects were not observed in primiparous cows. These results confirm that trace mineral sources affect apparent total-tract digestibility and indicate that milk productivity may also be affected.

Distinct responses in feed sorting, chewing behavior, and ruminal acidosis risk between primiparous and multiparous Simmental cows fed diets differing in forage and starch levels

During early lactation, both primiparous (PP) and multiparous (MP) cows are commonly fed diets rich in starch and low in forages to support their high energy requirements. Yet, the PP cows experience this dietary challenge for the first time, which might result in higher odds for them to develop rumen and systemic health disorders. The primary objective of this study was to evaluate the effect of decreasing the amount of forages in the diet on chewing and sorting behaviors and rumen and systemic health variables in PP and MP dairy cows. Twenty-four lactating Simmental cows [8 PP, average dry matter intake (DMI) of 19.1 ± 1.1 kg/d; 16 MP, average DMI of 22.5 ± 1.1 kg/d] with a body weight of 737 ± 90 kg and 50 ± 22 days in milk were used in this study. Cows were first fed a total mixed ration with 60% forage and 40% concentrate [on a dry matter (DM) basis] considered marginal in forages for 2 wk. Then, cows were switched to a diet low in forages with 40% forage and 60% concentrate (on a DM basis) for 4 wk. Reticular pH was measured continuously with wireless pH-sensors inserted into the reticulum to calculate the subacute ruminal acidosis (SARA) index. Chewing activity was measured with noseband-sensor halters, and feed sorting was measured weekly. Blood samples were collected weekly and analyzed for metabolic and inflammation markers. Switching PP and MP cows from a marginal to low-forage diet decreased the time spent eating and ruminating per kilogram of DM. Primiparous cows chewed longer per kilogram of DMI than MP cows. Also, the PP cows sorted more pronounced for longer particles and against fine particles than MP cows did. Despite higher rumination activity per kilogram of DMI and the adaptive sorting behavior, the PP cows spent on average 4.6 h/d longer below a pH of 5.8 and had a higher SARA index (i.e., area pH <5.8/DMI) than MP cows, especially during the first week of the low-forage diet (9.5 vs. 4.8). The concentration of liver enzymes increased with the low-forage diet, which was especially pronounced in the PP cows. In conclusion, this study demonstrated greater susceptibility of PP cows to SARA and liver damage than MP cows fed the same diets. Although PP cows demonstrated greater chewing and ruminating activity per kilogram of DMI, as well as adapted sorting behavior in favor of large particles during the low-forage high-starch feeding, they developed more severe signs of SARA. This suggests higher forage fiber requirements for PP cows and the need for improved feeding strategies to mitigate rumen fermentation disorders during early lactation in these cows.

Oilseed radish in single- and multi-crop sowings with poaceous crops

The results of field and laboratory research for 2015–2017 are presented and analyzed on the cultivation of traditional and uncommon poaceous crops. Recognized varieties of barley, oats, and millet were studied in single-crop sowings and intercropped with a high-protein cabbage crop – oilseed radish. The research was conducted on meadow chernozem mealy-carbonate soil in the forest-steppe zone of Trans-Baikal Territory. Agricultural technology used for fodder crop cultivation was common for this area. The objects of the research were the following varieties: barley Anna, oats Metis, millet Bystroe, oilseed radish Tambovchanka. The experimental work was carried out in accordance with the generally accepted guidelines for field experiments. Poaceous crops and oilseed radish were assessed in terms of their adaptability to growing conditions and their economically valuable characteristics that ensure sustainable production of high-energy feed. In multi-crop agrocenoses of poaceous crops and oilseed radish, the yield increased and the quality of feed raw material improved. The best results of multi-crop sowings were achieved by oats and millet intercropped with oilseed radish. The yield of green mass was 38.8-39.7 t/ha, dry matter content – 4.88-4.90 t/ha, digestible protein – 810820 kg/ha, and gross energy – 46.3-52.2 GJ/ha with availability of digestible protein – 180-186 g per one feed unit. Multi-crop sowings of oats and millet with radish oilseed are more productive than single-crop poaceous agrocenoses in feed units by 1.5-1.7 times, digestible protein by 2.9-3.3 times, gross energy by 1.2-1.6 times, with 10.5-10.7 MJ of exchangeable energy in one kilogram of dry matter.

Evaluation of vitamin D3 metabolites in Callithrix jacchus (common marmoset).

Vitamin D3 (cholecalciferol) is endogenously produced in the skin of primates when exposed to the appropriate wavelengths of ultraviolet light (UV-B). Common marmosets (Callithrix jacchus) maintained indoors require dietary provision of vitamin D3 due to lack of sunlight exposure. The minimum dietary vitamin D3 requirement and the maximum amount of vitamin D3 that can be metabolized by marmosets is unknown. Observations of metabolic bone disease and gastrointestinal malabsorption have led to wide variation in dietary vitamin D3 provision amongst research institutions, with resulting variation in circulating 25-hydroxyvitamin D3 (25(OH)D3 ), the accepted marker for vitamin D sufficiency/deficiency. Multiple studies have reported serum 25(OH)D3 in captive marmosets, but 25(OH)D3 is not the final product of vitamin D3 metabolism. In addition to serum 25(OH)D3, we measured the most physiologically active metabolite, 1,25-dihydroxyvitamin D3 (1,25(OH)2 D3 ), and the less well understood metabolite, 24,25-dihydroxyvitamin D3 (24,25(OH)2 D3 ) to characterize the marmoset's ability to metabolize dietary vitamin D3 . We present vitamin D3 metabolite and related serum chemistry value colony reference ranges in marmosets provided diets with 26,367 (Colony A, N = 113) or 8,888 (Colony B, N = 52) international units (IU) of dietary vitamin D3 per kilogram of dry matter. Colony A marmosets had higher serum 25(OH)D3 (426 ng/ml [SD 200] vs. 215 ng/ml [SD 113]) and 24,25(OH)2 D3 (53 ng/ml [SD 35] vs. 7 ng/ml [SD 5]). There was no difference in serum 1,25(OH)2 D3 between the colonies. Serum 1,25(OH)2 D3 increased and 25(OH)D3 decreased with age, but the effect was weak. Marmosets tightly regulate metabolism of dietary vitamin D3 into the active metabolite 1,25(OH)2 D3 ; excess 25(OH)D3 is metabolized into 24,25(OH)2 D3 . This ability explains the tolerance of high levels of dietary vitamin D3 by marmosets, however, our data suggest that these high dietary levels are not required.

Long spelling periods are required for pasture to become free of contamination by infective larvae of Haemonchus contortus in a humid subtropical climate of São Paulo state, Brazil

The objective of this trial was to evaluate the period of spelling necessary for a pasture to become free of contamination by infective larvae of gastrointestinal nematodes (GIN) of sheep, in different seasons of the year, as well as to determine when the greatest pasture contamination occurs and how long it lasts. An area was divided into four paddocks, one for each season (spring, summer, autumn, and winter). In order to contaminate the paddocks with free living stages of GIN, eight ewes, naturally infected, grazed on each paddock for 14 consecutive days, starting on the following dates: autumn, on April 4, 2017; winter, on July 4, 2017; spring, on September 26, 2017; summer, on January 2, 2019. At the beginning and end of the grazing period, faecal samples were taken directly from the rectums of the ewes to count eggs per gram of faeces (EPG) and for faecal cultures. Every 14 days pasture samples were collected to assess the number of infective larvae (L3) per kilogram of dry matter. At the end of the 14 day ewe grazing period, 21 stakes were placed where there were faeces on the paddock. Subsequently, every 14 days, the faeces located at three of the stakes were collected and the L3 were recovered. After the exit of the ewes, monthly, two tracer lambs, free of helminth infection, were allocated into the paddock for 14 days. At the end of this period they were housed in covered stalls for 28 days. Faeces from the lambs were collected for individual EPG counting and faecal culture at 21 and 28 days after grazing. Infective larvae recuperation was observed from faeces and pasture in all seasons. In the autumn, spring, and summer, high EPG counts were observed in the first tracer lambs (8521, 4800, and 8064 EPG, respectively), while in winter, high infection (14132 EPG) of the animals was observed only from the second pair of tracer lambs. For a pasture to become “clean”, 322 days, 350 days, 294 days, and 182 days following contamination were necessary, respectively, in the autumn, winter, spring, and summer. In autumn, spring, and summer, massive contamination of the pasture with L3 occurred soon after an area had been grazed by infected sheep, while in winter this took a little longer. The contamination persisted, approximately, from a minimum of six months post contamination in summer to up to almost one year post contamination in winter.

Gastrointestinal nematode infection in Purunã heifers raised in conventional and integrated livestock forest systems

ABSTRACT The objective of the study was to evaluate the resistance of Purunã heifers to nematode infection in two different production systems. In this study, 29 heifers were divided into eight pickets, four in an integrated livestock forest system (ILF) and four in a conventional pasture system (CPS). Feces were collected monthly to perform the fecal egg count (FEC), evaluate the degree of infection caused by gastrointestinal nematodes in cattle, and carry out coproculture, to identify the genus of the parasites. In addition, animal body weight was determined to evaluate performance. On the same day as the measurements, forage samples were collected to determine the number of infective larvae per kilogram of dry matter (L3/kg DM) in each picket. There were no statistical differences in FECs between the treatments. Although no significant differences were observed in the recovery of L3/kg DM, the contamination was higher in the ILF compared to CPS in December (344 and 37.7 L3/kg DM, respectively) and January (312 and 0 L3/kg DM, respectively). In March, the CPS (321 L3/kg DM) presented higher contamination than the ILF (80.7 L3/kg DM), but with no statistical difference. The weight of the animals was higher for the CPS compared to the ILF treatment throughout the whole experimental period, with a significant difference in December (343.30 kg and 314.08 kg, respectively). The present study demonstrated numerically that greater numbers of L3 were recovered from pasture of the ILF, which could have resulted in higher contamination and lower performance of the animals.

PSX-2 Growth and lactating performance of ewes fed with different energy levels

Abstract The objective of this study was to evaluate the milk production, body weight, and body condition score (BCS) of ewes fed with different energy levels. Seventy-one ewes were used and distributed in three treatments according to the metabolizable energy (ME, Mcal/kg) levels in the diet: CTL = according to the NRC (2007) recommendation for each stage (gestation and lactation); PLUS = CTL plus 10% of ME per kilogram of dry matter; LESS = CTL less 10% ME per kilogram of dry matter. Body weight and body condition score were evaluated a week before the expected date of parturition, a week after giving birth, and at weaning (60 days). Dairy production was evaluated at 20, 30, 40, 50 and 60 days after giving birth by the indirect method of the two weighing. The experimental design was analyzed as randomized complete blocks. There were no differences on milk production during almost all the lactation curve. Only on day 50 (P = 0.051), CTL was still at peak, while LESS and PLUS had already decreased their production. However, at 60 days, all treatments were already with low production, justifying weaning in this period. Results of weight and BCS showed a significant difference in all periods evaluates. Ewes in the PLUS diet had greater BW and BCS. The BCS before giving birth is expected to be over 3.5 and treatment LESS was the only one that did not achieve this score (2.66, 3.37, 3.83 for LESS, CTL, and PLUS, respectively). At weaning, ewes fed PLUS and CTL had BCS of 3.51 and 2.92, respectively, but treatment LESS had only 1.82. That is prejudicial for the next mating seasonal and this animal. We concluded that LESS diet interfered with the body reserves of sheep.

PSX-4 Performance of lambs born from ewes fed with different energy levels

Abstract The objective of this study was to evaluate the performance, under confinement conditions, of lambs born from ewes fed different energy levels during the final gestation and lactation period. Seventy-one ewes were distributed in two treatments according to the metabolizable energy (ME) levels in the diet: CTL = according to the NRC (2007) recommendations for each stage (gestation and lactation); PLUS = CTL plus 10% of energy per kilogram of dry matter. The total ME intake for the CTL group was 4 and 4.2 Mcal/day during the end of gestation and lactation, respectively, and for the Plus group, the ME intake were 4.8 and 5 Mcal/day. Thirty-five intact male lambs were weaned at 60 days and confined for an additional 60 days. In confinement, lambs were fed ad libitum with the same experimental diet their ewes received. The experimental design was analyzed as randomized complete blocks. The metabolic weight (BW0.75) at the confinement was influenced by maternal nutrition. Lambs of ewes fed with lower energy content were lighter compared to the PLUS group. At the beginning and the end of the confinement, the BW0.75 of the CTL was 8.80 and 15 kg0.75, and the PLUS was 11.4 and 17.6 kg0.75, respectively (P &amp;lt; 0.0001). The maternal diet containing higher energy content resulted in heavier carcass for commercialization. The values of weight gain and feed conversion were not influenced by maternal nutrition (P &amp;gt; 0.005), with mean values of 350 and 4.72 g/d, respectively. We concluded that lambs from ewes fed with greater energy intake had heavier body weights at the beginning and at the end of the confinement, possibly increasing the sustainability of the meat lamb production systems.

PSX-3 The impact of energy nutrition planes of ewes on the productive and reproductive performance of their offspring

Abstract The objective of this study was to evaluate different levels of metabolizable energy (ME) intake of late-gestating ewes on the productive and reproductive performance of their female offspring. Seventy-one crossbred ewes were distributed in three treatments: CTL = ME level according to recommendations of the NRC (2007); LESS = CTL less 10% ME per kilogram of dry matter (DM); and PLUS = CTRL plus 10% ME per kilogram of DM. Ewes were synchronized with hormonal protocol, controlled natural mating, and confirmed pregnancy using ultrasonographic imaging. The diets were given at the end of gestation (50 days) through weaning (60 days). The female progeny, from birth to the first mating season, received the same nutrition and sanitary management as their dams. They were weighed every 15 days from birth to the eighth month of age (first mating season), and the mortality rate was measured. The mating season lasted 34 days. The confirmation of pregnancy was performed with 30 and 60 days. The chi-square test was used to compare mortality and pregnancy rates, all with a 5% probability. Ewes in the LESS diet presented lower birth weight up to 8 months of age when compared to CTL and PLUS diets (P &amp;lt; 0.005), which were not different from each other (P &amp;gt; 0.005). Mortality was influenced by the maternal diet (P = 0.0017), with values of 57.1, 20, and 7.41% for the LESS, CTL, and PLUS groups, respectively. The pregnancy rate was 21.4, 70 and 55.6% for the LESS, CTL, and PLUS groups, respectively, with a difference between them (P = 0.0390). We concluded that the supply of low energy diets for dams caused losses in the performance and reproduction indexes of replacement ewes.