Kg Of Dry Matter Research Articles

Impact of Forage Sources on Ruminal Bacteriome and Carcass Traits in Hanwoo Steers During the Late Fattening Stages.

This study examined the effects of different forage sources on the ruminal bacteriome, growth performance, and carcass characteristics of Hanwoo steers during the fattening stage. In Korea, where high-concentrate feeding is common, selecting suitable forage is crucial for sustainable beef production. Fifteen 23-month-old Hanwoo steers, weighing an average of 679.27 ± 43.60 kg, were fed the following five different forage sources: oat hay (OAT), rye silage (RYE), Italian ryegrass (IRS), barley forage (BAR), and rice straw silage (RSS), alongside 1.5 kg of dry matter concentrate daily for five months. Carcass traits were evaluated post-slaughter, and rumen fluid samples were analyzed using full-length 16S rRNA gene sequencing to determine the bacteriome composition. The forage source significantly affected the alpha-diversity indices and bacteriome biomarkers linked to the feed efficiency and ruminal fermentation. Differences in the backfat thickness and meat yield index were noted, with alpha-diversity indices correlating with carcass traits. The phylum Planctomycetota, especially the family Thermoguttaceae, was linked to nitrogen fixation in high-protein diets like IRS, while the genus Limimorpha emerged as a biomarker for the meat yield. These findings highlight the importance of forage selection during late fattening to optimize beef production, considering diet and bacteriome shifts.

Effects of supplementing rumen-protected arginine on performance of transition cows

An experiment was conducted to determine the effects of supplementing rumen-protected arginine (RPA) on productive performance in dairy cows. One-hundred and 2 cows were blocked by parity and then by energy-corrected milk (ECM) yield. Within block, cows were randomly assigned to control (CON) that received 200 g/d of a mixture of hydrogenated soybean oil and heat-treated soybean meal to supply 30 g of metabolizable protein (MP), or 200 g/d of a product containing 30 g of metabolizable arginine (RPA), which increased the dietary arginine from 5.7 to 7.5% of the MP from 250 d of gestation to 21 d postpartum. After 21 d postpartum, cows were fed the same diet and data were collected until 84 d postpartum. Cows fed RPA produced an additional 2.5 kg of colostrum (5.3 vs. 7.8 ± 1.0 kg) and 220 g more immunoglobulin G (526 vs. 746 ± 93 g) than CON cows. Supplementing RPA increased the yields of milk (32.8 vs. 34.9 ± 1.0 kg/d), ECM (37.8 vs. 40.9 ± 1.2 kg/d), and milk total solids (4.48 vs. 4.86 ± 0.14 kg/d) in the first 21 DIM. The benefits of RPA extended beyond the period of supplementation, with a 6.4% increase in yield of ECM per kg of dry matter consumed in all cows (1.88 vs. 2.00 ± 0.05 kg/kg) and an increase in ECM yield, but only in parous cows (44.2 vs. 48.5 ± 1.5 kg/d). Feeding RPA increased the concentrations of urea N in plasma pre- (12.5 vs. 13.9 ± 0.4 mg/dL) and postpartum (11.6 vs. 13.2 ± 0.4 mg/dL), and in milk during the first 21 d postpartum (11.0 vs. 12.0 ± 0.3 mg/dL). Treatment did not affect the concentrations of AA in plasma prepartum, but feeding RPA tended to increase citrulline (72.5 vs. 77.5 ± 2.7 μM), whereas RPA either tended to decrease isoleucine (129.5 vs. 120.9 ± 5.7 μM) or decreased the concentrations of leucine (181.3 vs. 170.2 ± 6.4 μM) and valine (293.2 vs. 276.7 ± 10.4 μM) postpartum. Feeding RPA increased the relative expression of transcripts involved in AA transport (SLC38A4), urea cycle (ARG1), and gluconeogenesis (PC, PEPCK, and G6PC) in hepatic tissue. Feeding diets to supply additional arginine as RPA during the transition period benefited productive performance in dairy cows that extended beyond the period of supplementation despite minor changes in plasma AA concentrations.

Intestinal microbiota composition in broilers fed protein-free or casein-based diets

Growing broiler chickens of the Cobb500 strain were used to determine the effects on intestinal microbiota composition of a protein-free (PF) diet as compared to a diet based in casein (CAS) as the only protein source. CAS was formulated to contain the same amount of protein (190 g kg-1) as a commercial Maize-soy diet which was used as a practical reference. The ileal AA flow (g kg-1 dry matter intake) was significantly higher (P < 0.001) than PF in birds fed protein containing diets (CAS or Maize-soy). Taken as a whole (discriminant and ANOSIM analysis), the intestinal (ileal and caecal contents and ileal tissue) microbiota composition of PF and CAS were significantly (P < 0.001) different from Maize-soy and not different from each other in some cases. RT-qPCR and sequencing analysis of the ileal and caecal microbiota revealed significant (P < 0.05) differences in a number of bacterial groups between broilers fed PF, CAS or Maize-soy diets. The main result was that the lack of protein in the intestinal medium of PF birds resulted in a drop of Lactobacillus spp. counts (on average, 43 in PF vs 1,734 in the Maize-soy diet) and increased Enterobacteriaceae (on average, 419 in PF vs 172 in the Maize-soy diet) and other potentially pathogenic bacterial groups (in both intestinal contents and tissue). Thus, the lack of protein in the intestinal medium of PF birds resulted in a microbiota composition compatible with a pro-inflammatory state, and this effect was somewhat less marked in birds fed CAS. The results reported here suggest that the adverse effects on microbiota composition in broilers fed CAS were less marked than in those fed PF, which would be in line with a preferential use of a highly digestible protein containing diet to determine endogenous AA excretion instead of a PF diet.

Roe deer exposure to trace metals and pesticides in forests and agricultural plains of North-eastern France.

Human activities related to agriculture and industrial development result in the emission of different types of pollutants in the environment. The phytosanitary pressure depends on the time of the year, the type of habitat, and the used treatments. Wildlife, particularly ungulates, can be exposed to pesticides and trace metals through their herbivorous diet directly impregnated by the environmental contamination. Wild game thus plays a sentinel role but can also represent a risk for human health by exposing venison consumers to these contaminants. The present study explored this dual problem in two types of habitat: forests and agricultural plains located in North-eastern France. Samples of liver and muscle were taken from ten roe deer, five caught on the forests and five others in the plains, to determine contaminant levels. There was no significant difference in contamination of roe deer livers according to the habitat for three trace metals (chromium, nickel, and copper), while lead concentrations were higher in samples from the plain (0.85 vs. 0.74mg·kg-1 dry matter DM; P < 0.01). Conversely, zinc concentrations were higher in forest roe deer (110 vs. 95mg·kg-1 DM; P < 0.05). Cadmium concentrations did not differ significantly between the two habitats, but forest samples showed strong inter-individual variability, with some values close to those observed on the plains and others higher (1.8 vs. 0.3mg·kg-1 DM for forest and plain, respectively; P = 0.11). Mercury was not detected in any sample. The majority of pesticides investigated were not detected. Only a few pesticides were in trace amount (from 1 to 8µg·kg-1 fresh matter), either in the liver (e.g., heptachlor and oxychlordane) or in both tissues, liver and muscle (e.g., pyridalyl). Some compounds were only detected in plain (e.g., cypermethrin and fenpropidin) and others in forest (piperonyl butoxide and pyridalyl) samples. Analyses mainly detected insecticides in samples taken from forests and agricultural plains. Fungicides and a few herbicides were mainly detected in plain sampled animals. Pesticides and copper concentrations in roe deer tissues were lower than maximum residue levels established for farm (copper) or wild terrestrial (pesticides) animals. Cadmium concentrations were also lower than the maximum level for most samples, except for some forest roe deer. However, both forest and plain roe deer exceeded the maximum level for lead in liver. Human exposure was simulated according to different amounts of meat consumed and compared with toxicological reference values. This study highlighted an overall low risk to human health for both pesticides, copper and lead, with more reservations about cadmium for some roe deer shot in forest. The differences in roe deer contamination between forests and agricultural plains for some trace elements and organic pollutants confirmed the sentinel role of this species.

PSXII-6 Canopy height as a predictor of forage biomass in native rangelands and bermudagrass pastures

Abstract Accurate and less laborious estimates of forage biomass are needed for research and management decisions in grazing lands. Our objective was to develop linear regressions to estimate forage biomass using canopy height in both native rangelands and introduced bermudagrass [Cynodon dactylon (L.) Pers.] pastures in the Cross Timbers and Southern Tallgrass Prairie Ecoregion. Twelve pastures averaging 26 acres each were equally distributed in two ranches located in the Love County, Southern Oklahoma. The native rangelands comprise native tall- and mid-grasses along with forbs and some woody shrub species. Little bluestem [Schizachyrium scoparium (Michx.) Nash] is the dominant species, with brome (Bromus L.), broomsedge bluestem (Andropogon virginicus L.), ragweed (Ambrosia psilostachya DC.), purpletop tridens [Tridens flavus (L.) Hitchc.), dropseed (Sporobolus R. Br.), and Indiangrass [Sorghastrum nutans (L.) Nash] commonly found. The introduced pastures are dominated by bermudagrass with cereal rye (Secale cereale L.) and horseweed [Conyza canadensis (L.) Cronquist] commonly found as well. Samples occurred at pre- and post-grazing conditions from summer 2022 to fall 2023. Measurements included canopy height and hand-clipping all herbaceous vegetation in a 0.25-m2 frame. Forage samples were fresh weighed then dried for 72 h at 60°C and reweighed. There were 760 and 786 samples collected at native and introduced pastures, respectively. Data were analyzed using PROC GLM of SAS (SAS Inst., Cary, NC) including canopy height (cm) and forage biomass [kg of dry matter (DM)/ha] as independent and dependent variables, respectively. Only regressions including all parameters as significant (P &amp;lt; 0.05) were selected, and coefficient of determination (R2) and root mean square error (RMSE) were used to evaluate the predictive ability of the regressions. There was a pasture type (native or introduced) × season interaction (P &amp;lt; 0.001). This interaction is likely explained by the dominant species in each pasture type across seasons as well as their phenological stage. For the native rangeland, the spring linear regression including the quadratic term had the best fit with the greatest R2 (0.56) and the least RMSE (944 kg DM/ha; Table 1). Summer and fall regressions had R2 of 0.45 and 0.24 and RMSE of 1139 and 1343, respectively. No regressions were selected for winter because parameters were not significant (P = 0.53 and P = 0.18). For the introduced bermudagrass pasture, regressions presented R2 ranging from 0.24 and 0.30 except during the winter (R2 = 0.09). These results indicate that forage biomass can be acceptably estimated using canopy height only during spring in the native rangeland.

373 Effect of Cu and Zn contents in growing finishing pig diets on animal performance and Cu and Zn content in manure, according to three manure management chains

Abstract Copper (Cu) and zinc (Zn) are essential nutrients for swine. However, as pigs have a low retention rate for these elements, more than 90% of ingested Cu and Zn are excreted, mainly in feces. After spreading manure, these trace elements could accumulate in the soil and potentially have negative environmental effects. Moreover, Cu and Zn are also limited natural resources that should be preserved. A better understanding of the behavior of these elements throughout the feed-animal-effluent-treatment continuum according to feed composition and manure management chain is thus required to propose alternative ways to reduce these environmental impacts. Three feeding strategies differing in their Cu and Zn contents were compared in 72 growing-finishing pigs raised in individual pens [from 24.3 ± 3.3 up to 110 ± 9.0 kg body weight (BW), one-half males; and one-half females] a first diet with Cu and Zn at maximum EU regulation levels (20 and 100 ppm of Cu and Zn, respectively; REG), a second diet without any Cu and Zn supplementation (5 ppm Cu, 30 ppm Zn; WS) and a third diet with Cu and Zn at an intermediary level (10 and 50 ppm of Cu and Zn; INT). Cu and Zn were supplemented as Cu2O (CoRouge, Animine) and ZnO (HiZox, Animine), respectively. Eighteen additional finishing pigs (6 per diet) were raised in digestibility cages for excreta collection over 10 d, and their feces were submitted to two types of manure treatment: anaerobic digestion (AD) and composting with straw. Over the entire experimental period, the dietary Cu and Zn content did not influence growth performance of pigs. The Cu and Zn concentrations of the feces decreased linearly (P &amp;lt; 0.001) with dietary content from 191 and 834 (REG) up to 63 and 359 (INT) and 40 and 213 (WS) mg Cu and Zn/kg dry matter (DM) respectively. After both manure treatments, the degradation of the organic matter resulted in a significant increase (twice as much) in Cu and Zn concentration per kg of DM. Feeding strategy appears to be the main lever to reduce the Cu and Zn content of effluents. However, in the context of the diversification of manure management strategies, it is also important to take into account the effect of the effluent collection and treatment chain (e.g., spreading of raw slurry, phase separation, anaerobic digestion, composting), which has also an impact on the behavior of Cu and Zn and consequently on the risk for the environment.

18 Impacts of supplementing sulfate vs. hydroxychloride sources of Cu, Mn, and Zn on heifer growth performance and physiology during feed restriction and high-starch challenge periods

Abstract This study evaluated the effects of supplementing different sources (sulfate vs. hydroxychloride) of Cu, Mn, and Zn during feed restriction and high-starch challenge periods on heifer growth performance and physiology. On d 0, Nelore heifers (n = 40) were stratified by body weight (BW = 238 ± 38 kg) and age (21 ± 1 mo), and individually allocated into 1 of 40 drylot pens. The study was divided: washout (d 0 to 27), nutrient surplus (d 28 to 55), nutrient restriction (d 56 to 83), and high-starch challenge (d 84 to 112). The washout period consisted of limit-feeding Tifton hay (2.5% of BW; DM basis) and providing free choice access to white salt only. On d 28, heifers were randomly assigned to receive protein DM supplementation (0.10 to 0.20% of BW) added with sulfate (SUL) or hydroxychloride (HYD) sources of Cu, Mn, and Zn (≥ 260, 200, and 960 mg of Cu, Mn, and Zn per kg of supplement DM) from d 28 to 112 (n = 20 heifers/treatment). From d 28 to 55, heifers were offered ad libitum Tifton hay. From d 56 to 83, heifers were limit-fed 50% of average hay DM intake consumed in the last 6 d of the previous phase (d 50 to 55). From d 84 to 112, each respective protein supplement was mixed with a starch-based total mixed ration (TMR). Heifers were gradually adapted to the starch-based TMR by increasing the concentrate DM amount while simultaneously decreasing the hay DM amount by 20% every 7 d (starting with 35% concentrate and 65% hay on d 84 and ending with 80% concentrate and 20% hay from day 106 to 112). Individual heifer shrunk BW were collected on d 0, 28, 56, 84, and 112, following 12 h of feed and water withdrawal. Effects of treatment × day and treatment were not detected (P ≥ 0.37) for heifer BW, average daily gain (ADG), and dry matter (DM) intake, except for ADG from d 28 to 56, which was less (P = 0.05) for SUL vs. HYD heifers (-0.21 ± 0.05 vs. -0.10 ± 0.05 kg/day, respectively). Effects of day, but not treatment × day and treatment (P ≥ 0.43), were detected for fecal pH, which remained relatively constant from d 0 to 84, and then decreased (P &amp;lt; 0.01) from 7.2 on d 84 to 5.8-6.0 on d 110. Overall, protein supplementation added with hydroxychloride sources of Cu, Mn, and Zn had minor positive effects on heifer growth performance immediately following a washout period, but did not improve heifer growth performance and fecal pH during nutrient restriction and high-starch challenge periods, compared with protein supplementation added with sulfate sources of same metals.

Lemongrass essential oil in silage and as a feed additive has limited effect on animals feed intake and rumen fermentation

ABSTRACT This study aimed to evaluate the effects of lemongrass essential oil (LEO) addition during the ensiling and subsequent sheep feed intake, particle selection, in vitro rumen fermentation, total-tract apparent digestibility and nitrogen balance. Eight Dorper lambs 19.4±2.40 (mean±SD) kg of body weight were used in a trial using a 4 × 4 Latin Square design to evaluate: I. LEO addition during the whole-plant sorghum ensiling: 0 (EN−) and 4 (EN+) mL per kg of sorghum dry matter (DM); and II. LEO supply during the animals feeding: 0 (F−) and 1.6 (F+) mL per kg of diet DM. Treatments showed no effect on feed intake and particle size selection. Ensiling with LEO increased ammonia-N when LEO was not added during incubation. LEO addition during incubation decreased neutral detergent fibre (NDF) degradation when LEO was not added during the ensiling. In addition, LEO addition increased fermentation gas production and decreased digestibility. LEO addition in sheep feeding tended to decrease crude protein digestibility. Treatments did not affect nitrogen balance and the average daily gain. Thus, LEO supply during ensiling and sheep feeding does not affect feed intake, particle selection and nitrogen balance.

The Value of Hay Provided in Different Phases of Alfalfa Development

This article presents the results of research on the study of the chemical composition, digestibility, energy value of hay from alfalfa-rump grass mixture, harvested by the method of active aeration in different phases of alfalfa development, the productive properties of hay of different quality when fattening bulls in the dry steppe zone of the Nakhchivan. The amount of digestible energy decreased as the plants aged. Its highest concentration (12.8 MJ per 1 kg of dry matter) was observed during the budding phase. Then a decrease in this indicator was recorded by 0.8 MJ in the flowering phase and by 1.5 MJ during seed formation. The same pattern was noted for the content of metabolic energy in hay. Thus, the greatest amount was in hay harvested during the budding phase — 8.3 MJ per 1 kg. During the flowering phase, the amount of metabolic energy in 1 kg of hay decreased by 0.4 MJ, and seed formation by 1.2. Thus, the coefficient of digestibility of dry matter was higher than similar indicators during later harvesting by 4.01 and 7.05%, respectively. Crude protein in early harvested hay was digested better than in hay prepared in the phase of flowering and the beginning of alfalfa seed formation, by 11.83 and 18.03%, crude fiber — by 3.0-3.26%. A significant difference was also noted in digestion of raw fat. The digestibility of this substance decreased noticeably as plants aged. A decrease in the digestibility of nitrogen-free extractives was observed in hay during the seed formation phase. Calculations carried out on the basis of actual material obtained in the experiment show that when feeding hay harvested at the beginning of alfalfa budding, the yield of bull calves (increase in live weight) per 1 hectare of harvested area increases by 27.6% compared with harvesting in the flowering phase and 3.1 times in the seed formation phase.

Approach to the circular economy through agro-livestock waste composting with heat recovery and agricultural use of the resulting compost

In this work, plant waste and cow manure were co-composted to recover the heat generated during the thermophilic stage of composting by heating water through conducting heat exchangers buried in the pile. The parameters associated with the degradation and humification of organic matter during composting and the agronomic and economic value of the final compost were also determined. Furthermore, the effects of adding the obtained compost on soil agronomic characteristics and alfalfa yield and production profitability were compared with those of mineral fertilisation, cow manure and a control without amendment. The total energy recovered from composting was 14,528W or 0.105 MJ kg−1 dry matter of the initial waste mixture. Extracting heat from composting did not negatively affect the quality of the compost since this material had an adequate level of organic matter stability and maturity, notable nutrient content, low concentrations of potentially toxic elements and an absence of phytotoxicity. Moreover, applying compost to the soil increased its organic matter and nutrient content, contributing to greater alfalfa production. Increased alfalfa production led to a higher net income from using compost as an organic fertiliser (16,488 USD ha−1). Therefore, composting agricultural and livestock wastes is a viable method for managing these wastes, obtaining sustainable thermal energy and producing compost with adequate agricultural quality. This contributes to closing agricultural and livestock production cycles with notable economic benefits within a circular economy framework.

Effects of an essential oil blend rich in cinnamaldehyde and carvacrol on rumen biohydrogenation and fatty acid profile in the longissimus lumborum of growing lambs.

Essential oils extracted from cinnamon bark and oregano are rich in cinnamaldehyde and carvacrol and show potential for promoting animal performance. However, their impact on rumen biohydrogenation and the fatty acid composition of meat has not been reported. The hypothesis of this study was that a blend of essential oils rich in cinnamaldehyde and carvacrol would inhibit rumen biohydrogenation and promote the accumulation of polyunsaturated fatty acids (PUFAs) in lamb meat. The present study evaluated the effect of a blend essential oil (EO) rich in cinnamaldehyde and carvacrol on the nutrient digestibility, rumen biohydrogenation, growth performance, and fatty acid profile of the longissimus lumborum of lambs. Sixty male lambs with an average age of 84 ± 0.98 days and initial body mass of 25.4 ± 0.29 kg (mean ± standard deviation) were assigned randomly to four diets, and supplemented with 0 (EO0), 30 (EO30), 60 (EO60), and 120 (EO120) mg kg-1 dry matter of EO for 60 days. Although dry matter and neutral detergent fiber digestibility all showed a linear decrease (P ≤ 0.02) with increasing quantities of EO, final body mass and average daily gain increased linearly (P = 0.04), and average daily weight gain (ADG)/dry matter intake (DMI) tended to increase linearly (P = 0.07). Increasing EO supplementation resulted in a linear decrease in total volatile fatty acid concentration, acetate molar percentage, and acetate-to-propionate ratio (P ≤ 0.03), with the EO120 treatment being lower than the other EO treatments (P ≤ 0.05). Seven lambs from the EO120 treatment and seven lambs from the EO0 treatment were randomly slaughtered. It was observed that the proportions of C18:2n6c and PUFA in longissimus lumborum were higher in the EO120 treatment than the EO0 treatment (P ≤ 0.05). The relative abundance of Firmicutes in the rumen was decreased by the EO120 treatment in comparison with the EO0 treatment (P ≤ 0.05). Furthermore, the predicted relative abundances of genes encoding for conjugated linoleic acid reductase tended to decrease with the EO120 treatment (P = 0.06). We demonstrated that supplementation of the EO blend rich in cinnamaldehyde and carvacrol can enhance lamb growth performance and promote the deposition of desirable PUFAs in meat. © 2024 Society of Chemical Industry.

Tree size partially mediates the influence of climate on fruit and seed production in Ricinodendron heudelotii (Baill.) Heckel

AbstractUnderstanding environmental drivers of fruit and seed production in wild edible fruit species is essential for their valorisation. Ricinodendron heudelotii is an oilseed plant whose kernels are particularly treasured in cosmetic and medical industries. This study assessed fruits and seeds production patterns of R. heudelotii. Data were collected for three consecutive years on 30 trees in the phytodistricts of Plateau, Pobè and South Borgou where it naturally occurs. Tree dbh, total height, crown diameter, number of fruits and seed mass were measured. Climatic data were obtained from NASA Power Database. Data were analysed using linear (seed mass) and generalised linear (number of fruits) mixed models and structural equation modelling. Fruit and seed production were significantly higher in the South‐Borgou phytodistrict (1433 ± 1144 fruits, 4.26 ± 3.39 kg of Dry matter per tree) and Plateau phytodistrict (1.66 ± 0.35 g DM/seed). Tree dbh further significantly mediated the effect of minimum temperature, and relative humidity on seed mass. Tree dbh, irradiance and dry season rainfall were identified as the best‐predictors of seed mass production (kg DM). These results improved current knowledge of the fruit and seed production of R. heudelotii and are of significant importance for the exploitation of the species.

Accumulation Rate, Depuration Kinetics, and Tissue Distribution of Polychlorinated Dibenzo-p-Dioxins and Dibenzofurans (PCDD/Fs) in Suckler Ewes (Ovis aries).

Understanding the transfer of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in farm animals is essential for ensuring food safety, but such information for suckler ewes (Ovis aries) has been lacking. This work quantifies the accumulation, tissue distribution, and depuration kinetics of PCDD/Fs in these animals. Six suckler ewes (EXP group) were exposed to PCDD/Fs through contaminated hay (2.3-12.7 ng toxic-equivalent kg-1 dry matter) and then allowed to depurate by switching to noncontaminated hay from 29 days of lactation. Four control ewes were fed continuously with noncontaminated hay. At different time points covering depuration, weaning and slaughter, PCDD/F analysis of milk (three time points), blood and sternal adipose tissue (five time points), Longissimus thoracis muscle, liver, and empty body homogenate at slaughter (188 days of depuration) was performed. A relevant PCDD/F bioaccumulation was observed from oral intake in milk and adipose tissue (biotransfer factors of 1.24 and 1.06 day kg-1 lipids for the sum toxic-equivalent, respectively) in the EXP ewes, especially for penta- and hexa-chlorinated congeners. The EXP ewes' adipose tissue started at 10-fold the EU maximum level (ML) and showed depuration below the ML after 130 days. Specific PCDD/F accumulation in the ewe liver was observed, especially for dibenzofurans. These toxicokinetic data can inform recommendations to ensure the chemical safety of sheep food products.

Effects of harvest date and additives on maize silage quality under boreal conditions

There is increasing interest in Finland to cultivate maize for silage, although the climatic conditions are in the borderline for maize due to the short and cool growing season. This may result in an immature crop that differs from typical maize for ensiling by having low dry matter (DM) and starch concentrations. We evaluated the preservation characteristics of forage maize during 2019 and 2020 harvested in Helsinki, Finland, at two stages of maturity. The DM concentration of the crops ranged from 230 to 360 g kg-1, and starch concentration from 179 to 283 g kg-1 DM. The crops were ensiled in laboratory scale using four different chemical organic acid based additives or a heterofermentative lactic acid bacteria inoculant. A control silage without additive treatment was also included. All silages were well fermented with low pH (average 3.75) and proportion of ammonia N in total N (average 47 g kg-1). Formic acid based additives restricted silage fermentation and most chemical additives improved the aerobic stability of maize silages compared to the control and inoculant treated silages that, under the conditions of the current study, did not differ from each other.

Process and quality evaluation of different improved composts made with a smart laboratory pilot plant

This study monitored the process and investigated the quality of compost obtained from different biomasses. Five blends of agri-food waste were composted by a laboratory pilot plant named COMPOSTER, that is designed to optimize biodegradation, and produce compost efficiently. The COMPOSTER consists of two 35-liter nearly adiabatic, aerated bioreactors that simulate an industrial process involving the typical sequence of mesophilic-thermophilic-mesophilic phases. It continuously monitors and records temperature, internal pressure, and biomass weight, while controlling and quantifying oxygen consumption and carbon dioxide emissions resulting from aerobic biodegradation. All composts were characterized for their main chemical, physical, and molecular features, as well as their suppressiveness against Fusarium oxysporum f.sp. lycopersici (FOL), tested on tomato seedlings. Optimized biodegradation yielded 50–60 % mature compost with a cumulative oxygen consumption ranging from 282 to 456 gO2 per kg of dry matter, with peaks of 2.55 gO2 per kg of volatile solids per hour, and carbon dioxide emissions of 22–36 % of the initial carbon content, with peaks of 5.89 g CO2 per kg of volatile solids per hour. Blends containing more ligno-cellulosic ingredients showed higher yields and lower CO2 emissions. Most of the nitrogen present initially was retained in the final compost; indeed, all mixtures exhibited an apparent nitrogen concentration increase due to carbon loss. Composting determined deep modifications in the molecular structure of the organic matter. 13C CPMAS-NMR and off-line thermochemolysis GC-MS analyses highlighted decomposition degree of polysaccharides and peptidic moieties, selective preservation of aliphatic and aromatic recalcitrant compounds, and optimal ongoing humification. All composts were non-phytotoxic, except for that including pepper crop residues, and all resulted rich in macro- and micro-elements for plant nutrition and proved to be active in controlling FOL disease. Compost comprising 81.2 % tomato crop waste exhibited the best growth performance and pathogen control on tomato. Mature, non-phytotoxic, nutrient-rich, and suppressive composts represent promising by-products that can be successfully recycled in agriculture, including high-value applications, leading to lower use of fertilizers and pesticides.

Effects of Palm Oil Deodorizer Distillate on the Ruminal Environment of Sheep.

This study aimed to assess the impact of palm oil deodorizer distillate (POD) on the ruminal environment, including (i) microbial community, (ii) ruminal degradability, and (iii) apparent digestibility in sheep. The data used were derived from twenty rumen-cannulated sheep fed five isoproteic and isofiber diets based on elephant grass (Pennisetum purpureum Schum. cv. Roxo) silage supplemented with 0, 25, 50, 75, or 100 g kg-1 POD on a dry matter (DM) basis. Rumen fluid samples were collected three hours after feeding directly from the ventral sac of the rumen via a cannula and then subjected to DNA extraction, which was subsequently used for 16S rDNA amplification, followed by sequencing and diversity analysis. In this study, the microbial diversity was dominated by Bacteroidetes and Firmicutes, followed by Euryarchaetoa, Actinobacteria, and Tenericutes, in the ruminal environment, and was slightly modified when supplemented with the POD up to 100 g/kg (10%), leading to only a slight decrease in the diversity index. The ruminal degradability, ruminal fermentation parameters, and apparent digestibility were slightly compromised by the inclusion of up to 25 g of POD per kg of DM, and larger inclusions interfered with the ruminal degradability of fibrous fractions and the apparent digestibility of dry matter. This lipid supplement showed good results for feeding sheep and is an inexpensive and abundant alternative in the regional market.

Productive grass stands of pasture use for conditions of the Vologda region

Scientific research was carried out on the experimental field of the North-Western Research Institute of Dairy and Grassland Farming, a separate division of the Voluntary Research Center of the Russian Academy of Sciences, located in the village of Dityatevo, Vologda Region. The soil of the experimental plot is soddy-podzolic and moderately cultivated. Pasture is a cheap and accessible food source for herbivores; it solves the problems of survival in crisis ecological, economic, and atypical climatic conditions. Pasture farming has significant animal health benefits, allowing them to consume desirable grass species. In the Russian Federation, pastures occupy about 30% of the area of agricultural land, and in the Vologda region, they occupy 13.3% (145.3 thousand hectares). The article describes ten years of research on the creation of pastures based on traditional and rare perennial grasses. In the composition of pasture grass stands, we studied cereal grasses (meadow timothy grass Vologda local and Leningradskaya 204, meadow fescue Sverdlovskaya 37, festulolium Allegro, perennial ryegrass VIK-66, awnless brome SibNIISKhoz-189, meadow bluegrass Limagi and Dar) and legumes (eastern goat’s rue Krivich, Lyad crown horned Solnyshko, meadow clover Carmine and Dymkovsky, creeping clover Belogorsky and Lugovik). Cereal and legume-cereal grass stands on average for 2011–2015. provided yields from 1.3 to 5.3 t/ha, metabolic energy output – 12.6–52.5 GJ/ha, and crude protein content ranging from 10.3 to 15.6% per 1 kg of dry matter; in 2017–2021, these figures were 2.3–8.4 t/ha, 23.4–87.6 GJ/ha, 13.4–18.9%, respectively.

Sunflower oil supplementation in the diets of lactating cows: productive and nutritional performance

Eight Jersey cows (2nd-4th lactation; 483 ± 43 kg body weight; milk yield 21±2.2 kg day-1) were used in a double 4x4 latin square design to evaluate whether the inclusion of increasing levels of sunflower oil in the concentrate up to the limit of 110.0 g kg-1 DM of EE, in replacement to corn grain, has an impact on milk production and composition, feed efficiency, energy balance, intake and digestibility. of diets, and the fatty acid profile of milk fat from Jersey cows. The treatments consisted in lipid supplementation with increasing levels of sunflower oil replacing the corn grain and wheat bran of concentrate, including: CD (control diet), without sunflower oil and with 38 g kg-1 of EE dry matter (DM); and three treatmens with sunflower oil concentrate-included: SF65= 65 g kg-1 DM of EE; SF86=86 g kg-1 DM of EE and SF110=110 g kg-1 DM of EE. The increase of EE in diets did not affect the dry matter intake, neutral detergent fiber or crude protein. There was a linear increase in the digestibility coefficients for organic matter, crude protein, EE, neutral detergent fiber and blood levels of cholesterol. Treatments did not affect milk production, fat content, lactose or total solids. It is possible to use up to a limit of 110.0 g kg-1 DM of EE in the diet, decreasing the concentrate total amount.

Türkiye'nin Orta Anadolu Bölgesinde Bazı Silajlık Mısır Hibritlerinin Tarımsal ve Besin Profilinin Gözlenmesi

The silage corn (SC) production and yield per area in Turkey have increased over the last decade owing to their high economic importance. The first objective of the current study was to determine the agronomic and nutritive profiles of SC hybrids in the Central Anatolian region of Ankara, Turkey. The other objective was to determine the relationship between silage maturity and corn heat unit (CHU) and growing degree day (GDD) ratings. The current study was set up as a randomized complete block design with three replications. Certified seeds of five dissimilar SC hybrids (cv PL 712, cv TORRO, cv CHAMP, cv MACHA, cv RANGER) (n=5) were established in the field. SC hybrids showed variation among themselves. Differences were also detected in all parameters, except dry matter (DM) content and crude protein (CP) yield. Cv “MACHA” had the highest nutritive profile [CP: 9.60%; acid detergent fiber (ADF): 30.02%; neutral detergent fiber (NDF): 51.62%; acid detergent lignin (ADL): 3.72%; total digestible nutrient (TDN): 62.60%; metabolic energy (ME): 2.34 Mcal kg-1 DM; net energy production (NEp): 1.50 Mcal kg-1 DM]. One of the other hybrids, Cv “CHAMP” had the highest yield components [silage yield: 104.20 t ha-1; dry matter yield: 35.4 t ha-1; TDN yield: 21.10 t ha-1; ME yield: 77880.00 Mcal ha-1; NEp yield: 49914.00 Mcal ha-1]. There was a significant correlation between silage maturity and CHU and GDD ratings [CHUseed (r = 0.84, P= 0.001), CHUsilk (r = 0.90, P

The composition and nutritional quality of biorefined lucerne protein depend on precipitation method.

Lucerne protein extract is a novel high-quality protein source with excellent amino acid (AA) composition of interest for human consumption. In this study, protein from screw-pressed lucerne juice was extracted by different precipitation methods to evaluate the effect on the chemical composition and nutritional quality of the extracted protein. Methods based on heat, acidification or fermentation were used for protein precipitation, and the nutritional value of protein was evaluated in a rat digestibility trial. Heat precipitation at 85 °C produced a protein product with a crude protein (CP) content of 589 g kg-1 dry matter (DM), a balanced AA composition and a high standardized nitrogen (N) digestibility (82.8%). Precipitation by acidification, at a lower temperature (60 °C) or by fermentation, resulted in lower CP content (425-488 g kg-1 DM). Nitrogen digestibility for the pH-adjusted precipitate was equal to the 85 °C heat-precipitated protein, while the fermented and 60 °C precipitated proteins showed lower N digestibility (76.5% and 78.6%, respectively). By applying a two-step heat precipitation method (60 °C followed by 80 °C), a protein content of 712 g kg-1 DM and an N digestibility of 93.6% was reached, which are comparable to high-quality animal-based protein sources such as milk, whey, casein, and eggs, covering the AA requirements for children >6 months. High-quality protein can be extracted from lucerne, but the future focus should be on increased yield as the current low yields of the refined product will challenge the environmental and economic sustainability of production. © 2023 Society of Chemical Industry.