Key Sites Research Articles

Spatial distribution and dynamics of thermocirques in a key area of Central Yamal based on remote sensing data

Т. In recent decades, the distribution and activation of thermodenudation, which leads to the formation of specific landforms — thermocirques (also referred to as retrogressive thaw slumps, RTS), have been intensively studied. In different regions of the cryolithozone and at different time intervals, both activation and stabilization of thermocirques are observed. As a rule, studies focus on the climatic controls of the phenomena observed, the environmental controls are discussed less often. This study presents an analysis of the dynamics of thermocirques in relation to the relief features in a specific key area of Central Yamal. To achieve this aim, the spatial distribution of thermocirques at different geomorphological levels is considered based on multi-temporal remote sensing data. Satellite images obtained in 2009, 2018, and 2023, as well as a global digital elevation model (ArcticDEM), were used. We outlined five geomorphic levels and determined their parameters: area, altitude, steepness, and the aspect of the slopes. Thermocirques were identified in the images and their parameters were measured. The dynamics of the thermocirques were analyzed by their number, area, length, width, slope aspect and angle for the periods 2009–2018 and 2018–2023, and for 14 years in total, separately for each geomorphic level. It was found that thermocirques predominate on the slopes of the III alluvial-marine plain, 5–12° steep. In 14 years, the total area of thermocirques increased by 296 %, and their number — by 61 %. A larger increase in the total area and number of thermocirques occurred during the period 2009–2018 in response to climate extremes in 2012 and 2016. Thermocirques that face west cover a higher total area, partly due to the predominance of such slopes over the area of the key site. In all the years of observation, the average areas and lengths of thermocirques are maximum on south-facing slopes. Some of the results are close to those obtained in other regions of Russia and in North America. In many of the areas studied, the increase in the total area of thermocirques exceeded the increase in their number, which means that the expansion of the existing forms prevails over the inception of the new ones. The discrepancies observed in different studies in the results of assessing the effect of relief on thermocirques are due to both the regional features and differences in satellite imagery and methods of its processing.

Composition of Marine Debris on The Coast of Banyuwangi, East Java

Marine debris has emerged as a significant environmental issue of growing global concern, closely linked to pollution and its impact on the environment. Understanding the distribution of marine waste in coastal areas is crucial, especially considering that beaches are key sites for various human activities such as tourism, fishing, and shipping. To address this, a study was conducted to analyze the composition of marine debris on three beaches in Banyuwangi Regency, East Java. The study took place in July and August of 2023, focusing on Boom, Cacalan, and Cemara beaches. Marine debris was collected along the coastline in 5 x 5 m transects, spaced 20 m apart, to identify and quantify the types of waste. The findings revealed that Boom Beach had the highest percentage of rubbish (46%), followed by Cemara Beach (30%) and Cacalan Beach (24%). Additionally, the highest abundance of rubbish per area was found at Boom Beach (5.97 ± 16.64 item/m2), Cemara Beach (3,92 ± 11,36 item/m2), and Cacalan Beach (3,12 ± 5,27 item/m2). Boom Beach and Cemara Beach were primarily dominated by plastic waste, while at Cacalan Beach, plastic waste and broken glass were found in nearly equal proportions. The calculation of the Clean Coast Index (CCI) indicated that all three beaches were significantly polluted. As a result, there is a clear need for focused attention on waste management at tourist beaches to preserve the aesthetics and comfort of beach visitors.

Unearthing Identity: the Role of Archaeology in Shaping English National Identity and Heritage Interpretation

ABSTRACT This study examines the significant impact of archaeology on English national identity and heritage interpretation, focusing on key sites like Stonehenge, Sutton Hoo, and Hadrian’s Wall. Using a multidisciplinary approach, we analyze how archaeological discoveries shape national narratives, emphasizing continuity, rootedness, and cultural uniqueness. Through qualitative methods, including interviews and focus groups, we find that public perceptions of these sites highlight themes of engagement, inclusivity, and the need for more comprehensive interpretations. Our results reveal that archaeological interpretations evolve with societal values and political agendas, often reinforcing or challenging notions of national identity. This study underscores the dynamic relationship between archaeology, nationalism, and cultural memory, advocating for a more inclusive and nuanced approach to heritage management and public engagement.

Molecular Mechanisms Underlying the Spectral Shift in Zebrafish Cone Opsins.

Visual pigments are essential for converting light into electrical signals during vision. Composed of an opsin protein and a retinal-based chromophore, pigments in vertebrate rods (Rh1) and cones (Rh2) have different spectral sensitivities, with distinct peak absorption wavelengths determined by the shape and composition of the chromophore binding pocket. Despite advances in understanding Rh1 pigments such as bovine rhodopsin, the molecular basis of spectral shifts in Rh2 cone opsins has been less studied, particularly the E122Q mutation, which accounts for about half of the observed spectral shift in these pigments. In this study, we employed molecular modeling and quantum mechanical techniques to investigate the molecular mechanisms behind the spectral difference in blue-shifted Rh2-1 (absorption peak = 467 nm, 122Q) and green-shifted Rh2-4 (absorption peak = 505 nm, 122E) zebrafish cone opsins. We modeled the pigments 3D structures based on their sequences and conducted all-atom molecular dynamics simulations totaling 2 microseconds. Distance analysis of the trajectories identified three key sites: E113, E181, and E122. The E122Q mutation, previously known, validates our findings, while E181 and E113 are newly identified contributors. Structural analysis revealed key features with differing values that explain the divergent spectral sensitivities of Rh2-1 and Rh2-4: 1) chromophore atom fluctuations and C5-C6 torsion angle, 2) binding pocket volume, 3) hydration patterns, and 4) E113-chromophore interaction stability. Quantum mechanics further confirms the critical role of residue E181 in Rh2-1 and E122 in Rh2-4 for their spectral behavior. Our study provides new insights into the molecular determinants of spectral shifts in cone opsins, and we anticipate that it will serve as a starting point for a broader understanding of the functional diversity of visual pigments.

Building Resilience through Territorial Planning: Water Management Infrastructure and Settlement Design in the Coastal Wetlands of Northern Apulia (Salpia vetus-Salapia) from the Hellenistic Period to Late Antiquity

This Gulf of Manfredonia has, for millennia, been the primary water feature of the coastal wetland of Northern Apulia, Italy, although modern reclamation works make writing its long-term history challenging. Our recent paleoenvironmental research has reconstructed the evolution of the southern half of this lagoon since the Neolithic period. Here, we write a history of water management and environmental change in this landscape from the perspective of two key urban sites: pre-Roman Salpia vetus and Roman Salapia. The Roman architectural historian Vitruvius recounts the abandonment of Salpia vetus and the refoundation of Salapia. We employ his narrative as a frame for a more complex environmental history, starting from a historiography of this landscape’s study and a summary of our interdisciplinary research agenda, which unifies environmental, topographical, remote sensing, and archaeological approaches. Resilience in this changeable wetland environment was only possible through an integrated and intentional management of water among rivers, the lagoon, and the Adriatic Sea. While Salpia vetus exploited this wetland and thrived for centuries, the settlement eventually collapsed due to human and environmentally impelled factors. Roman Salapia subsequently emerged with a different approach, new infrastructure, and a new location. This blueprint would sustain urban life in this wetland for six centuries and lay the groundwork for the Medieval town.

CcCHIL, a type IV chalcone isomerase that can improve (2S)-naringenin production in Saccharomyces cerevisiae

(2S)-Naringenin, a crucial precursor in plant flavonoid synthesis, holds significance in diverse biological processes and potential therapeutic applications for human diseases. The economically valuable Citrus reticulata 'Chachi' citrus cultivar, renowned for its flavonoid-rich peel, was subjected to integrated transcriptomic and metabolomic analysis in this study to reveal patterns of flavonoid accumulation. The analysis revealed a strong correlation between the expression pattern of the gene encoding type IV chalcone isomerase (chalcone isomerase like, CHIL), CcCHIL, and the accumulation of flavonoids in C. reticulata 'Chachi' peel. Previous studies have demonstrated that type IV chalcone isomerase can enhance flavonoid accumulation in citrus and improve the catalytic efficiency of chalcone synthase in vitro, thereby increasing the titer of (2S)-naringenin. In this study, we constructed a genetically engineered yeast strain capable of de novo synthesis of (2S)-naringenin. We found that CcCHIL can increase (2S)-naringenin production in engineered yeast by 13.60 %. Subsequently, we conducted experiments using CHILs from various species and found that GmCHIL from Glycine max can increase (2S)-naringenin production in engineered yeast by 45.35 %. Sequence alignment, molecular docking predictions and site-directed mutagenesis showed that Asn151 is one of the key sites for CHIL improving (2S)-naringenin production. Our study unveiled CcCHIL's crucial role in flavonoid biosynthesis in C. reticulata 'Chachi' peel and paving the way for future synthesis of intricate and economically valuable flavonoids.

A numbers game: mosquito-based arbovirus surveillance in two distinct geographic regions of Latin America.

Aedes mosquitoes, as vectors of medically important arthropod-borne viruses (arboviruses), constitute a major public health threat that requires entomological and epidemiological surveillance to guide vector control programs to prevent and reduce disease transmission. In this study, we present the collaborative effort of 1 year of Aedes ageypti (Linnaeus, 1762) mosquito-based arbovirus surveillance in 2 geographically distinct regions of Latin America (Nicaragua and Ecuador). Adult female mosquitoes were collected using backpack aspirators in over 2,800 randomly selected households (Nicaragua, Ecuador) and 100 key sites (Nicaragua) from 8 distinct communities (Nicaragua: 2, Ecuador: 6). A total of 1,358 mosquito female pools were processed for RNA extraction and viral RNA detection using real-time reverse transcription-polymerase chain reaction. Ten positive dengue virus (DENV) pools were detected (3 in Nicaragua and 7 in Ecuador), all of which were found during the rainy season and matched the serotypes found in humans (Nicaragua: DENV-1 and DENV-4; Ecuador: DENV-2). Infection rates ranged from 1.13 to 23.13, with the Nicaraguan communities having the lowest infection rates. Our results demonstrate the feasibility of detecting DENV-infected Aedes mosquitoes in low-resource settings and underscore the need for targeted mosquito arbovirus sampling and testing, providing valuable insights for future surveillance programs in the Latin American region.

Systematic genetic characterization of the human PKR kinase domain highlights its functional malleability to escape a poxvirus substrate mimic.

Evolutionary arms races can arise at the contact surfaces between host and viral proteins, producing dynamic spaces in which genetic variants are continually pursued. However, the sampling of genetic variation must be balanced with the need to maintain protein function. A striking case is given by protein kinase R (PKR), a member of the mammalian innate immune system. PKR detects viral replication within the host cell and halts protein synthesis to prevent viral replication by phosphorylating eIF2α, a component of the translation initiation machinery. PKR is targeted by many viral antagonists, including poxvirus pseudosubstrate antagonists that mimic the natural substrate, eIF2α, and inhibit PKR activity. Remarkably, PKR has several rapidly evolving residues at this interface, suggesting it is engaging in an evolutionary arms race, despite the surface's critical role in phosphorylating eIF2α. To systematically explore the evolutionary opportunities available at this dynamic interface, we generated and characterized a library of 426 SNP-accessible nonsynonymous variants of human PKR for their ability to escape inhibition by the model pseudosubstrate inhibitor K3, encoded by the vaccinia virus gene K3L . We identified key sites in the PKR kinase domain that harbor K3-resistant variants, as well as critical sites where variation leads to loss of function. We find K3-resistant variants are readily available throughout the interface and are enriched at sites under positive selection. Moreover, variants beneficial against K3 were also beneficial against an enhanced variant of K3, indicating resilience to viral adaptation. Overall, we find that the eIF2α-binding surface of PKR is highly malleable, potentiating its evolutionary ability to combat viral inhibition.

Identification of Cables1 as a critical host factor that promotes ALV-J replication via genome-wide CRISPR/Cas9 gene knockout screening

Avian leukosis virus subgroup J (ALV-J), a member of the genus Alpharetrovirus, possesses a small genome and exploits a vast array of host factors during its replication cycle. To identify host factors required for ALV-J replication and potentially guide the development of key therapeutic targets for ALV-J prevention, we employed a chicken genome-wide CRISPR/Cas9 knockout library to screen host factors involved in ALV-J infection within DF-1 cells. This screening revealed 42 host factors critical for ALV-J infection. Subsequent knockout assays showed that the absence of the genes encoding cycle-regulatory proteins, namely Cables1, CDK1, and DHFR, significantly inhibited ALV-J replication. Notably, Cables1 knockout cell lines displayed the most pronounced inhibitory effect. Conversely, overexpression assays confirmed that Cables1 significantly promotes ALV-J replication. Immunoprecipitation assays further indicated that Cables1 specifically interacts with the viral protein p15 (viral protease) among all ALV-J proteins, enhancing ALV-J p15 polyubiquitination. Additionally, we identified 26 lysine residues of ALV-J p15 as key sites for ubiquitination, and their replacement with arginine attenuated the replication ability of ALV-J in both in vitro and in vivo assays. This study demonstrates that Cables1 is a critical replication-dependent host factor of ALV-J by enhancing p15 ubiquitination and thereby promoting viral replication. Overall, these findings contribute to a deeper understanding of the ALJ-V replication mechanism and offer a potential target for the prevention and control of ALV-J infection.

Stone Tools in Shifting Sands: Past, Present, and Future Perspectives on the Châtelperronian Stone Tool Industry

The Middle to Upper Palaeolithic transition in France and northern Spain reflects the transition from Neandertals to Homo sapiens and the emergence of novel cultural entities and standardised blade and bladelet technologies between ~ 55 and 40 thousand years ago. The Châtelperronian stone tool industry represents the first unambiguous appearance of Upper Palaeolithic technologies in this region, and is traditionally considered as representing a geographically isolated archaeological entity produced by late Neandertals. However, debate as to the makers and origin of this industry has been ever-present. In recent years, fuel has been thrown onto this discussion through (a) the demonstration that the association between Neandertal remains and Châtelperronian artefacts at the key site of Saint-Césaire could not be reliable, (b) the identification of an immature Homo sapiens pelvic fragment in association with Neandertal remains and Châtelperronian artefacts at the Grotte du Renne (Arcy-sur-Cure), and (c) the formulation of a disruptive hypothesis in which the Châtelperronian directly originates from the Early Upper Palaeolithic of the Levant. In conjunction with the increasing evidence for a protracted presence of Homo sapiens across Europe, these observations have led to the arrival of an inflection point for the competing interpretations concerning the origin and implications of this industry. In this paper, we provide a critical review of the Châtelperronian in light of the emerging data—taking into account technological, chronological, geographic, stratigraphic, and genetic perspectives. First we provide a detailed, three-part historiography of this industry and a modern, synthetic review of Châtelperronian lithic technology. Our review reinforces the fact that the Châtelperronian is a fully Upper Palaeolithic industry with no ‘transitional’ nor Initial Upper Palaeolithic-type technological features. Subsequently, we highlight a series of prospects, problems, and uncertainties which remain to be addressed in discussions concerning the origins, maker(s), and implications of the Châtelperronian and the onset of the Upper Palaeolithic in western Europe. Finally, we propose a few potential paths forward and call for an open and critical approach towards the re-conceptualisation of the Châtelperronian in the years to come.

Two Structural Analogs of Kairomones are Detected by an Odorant Receptor HvarOR28 in the Coccinellid Hippodamia variegata.

In natural environments, general plant volatiles and herbivore-induced plant volatiles (HIPVs) serve as critical clues for predatory natural enemies in the search for prey. The insect olfactory system plays a vital role in perceiving plant volatiles including HIPVs. In this study, we found that HIPV (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT) and the plant volatile geranyl acetate (GA), two structurally similar chemicals, displayed electrophysiological activities on the antennae of the ladybird Hippodamia variegata, but were only attractive to adult females in behavior. Moreover, mated female ladybirds laid a significantly higher number of eggs on TMTT-treated and GA-treated cotton leaves compared to controls. Screening of female-biased odorant receptors (ORs) from the antennal transcriptomes, performing Xenopus oocytes expression coupled with two-electrode voltage clamp recordings, suggested that HvarOR28 specifically tuned to TMTT and GA. Molecular docking and site-directed mutagenesis revealed that the amino acid residues Tyr143 and Phe81 of HvarOR28 are the key site for binding with TMTT and GA. Furthermore, RNA interference (RNAi) assay demonstrated that HvarOR28-silenced individuals demonstrated a notable decrease in electrophysiological responses, even female adults almost lost behavioral preference for the two compounds. Thus, it could be concluded that HvarOR28 in H. variegata contributes to facilitating egg laying through the perception of TMTT and GA. These findings may help to develop new olfactory modulators based on the behaviorally active ligands of HvarOR28.

Analysis of the coupling and coordination between soil erosion and land use in the Northeastern black soil region of China: a case study of Lishu County

Lishu County, which is located in the black soil region of Northeast China, represents a key site for the analysis of soil erosion intensity. This study offers a scientific foundation for the development of targeted soil and water conservation strategies within the region. The Revised Universal Soil Loss Equation (RUSLE) was employed to compute the soil erosion modulus in Lishu County, with the objective of conducting a quantitative analysis of the temporal and spatial distribution patterns of soil erosion. Additionally, the changing characteristics of soil erosion were examined from the perspectives of land use types and slope variations. The Generalized Connectivity Causality Model (GCCM) was utilized to identify the causal relationship between soil erosion and land use types through the reconstruction of state space and cross-mapping predictions. (1) Soil erosion in Lishu County between 2000 and 2020 predominantly exhibited mild to moderate levels, characterized by patchy and sporadic erosion, with relatively severe occurrences in the northern and central regions. (2) Soil erosion was correlated with land use and slope variations, with more than 90% of erosion incidents transpiring in cultivated land areas. The 3°-5° slope range in Lishu County emerged as a focal point for erosion, necessitating targeted prevention and control measures. (3) The GCCM model illustrated a discernible causal relationship between soil erosion and land use, revealing mutual influences between the two factors. Between 2000 and 2020, both the area and intensity of soil erosion in Lishu County exhibited an initial increase, followed by a subsequent decrease. This suggests an overall trend of amelioration in soil erosion conditions. However, notable spatial disparities persist in the erosion distribution across the region.

Design, Synthesis, and Biological Evaluation of Darunavir Analogs as HIV-1 Protease Inhibitors.

Darunavir, a frontline treatment for HIV infection, faces limitations due to emerging multidrug resistant (MDR) HIV strains, necessitating the development of analogs with improved activity. In this study, a combinatorial in silico approach was used to initially design a series of HIV-1 PI analogs with modifications at key sites, P1' and P2', to enhance interactions with HIV-1 PR. Fifteen analogs with promising binding scores were selected for synthesis and evaluated for the HIV-1 PR inhibition activity. The variation of P2' substitution was found to be effective, as seen in 5aa (1.54 nM), 5ad (0.71 nM), 5ac (0.31 nM), 5ae (0.28 nM), and 5af (1.12 nM), featuring halogen, aliphatic, and alkoxy functionalities on the phenyl sulfoxide motif exhibited superior inhibition against HIV-1 PR compared to DRV, with minimal cytotoxicity observed in Vero and 293T cell lines. Moreover, computational studies demonstrated the potential of selected analogs to inhibit various HIV-1 PR mutations, including I54M and I84V. Further structural dynamics and energetic analyses confirmed the stability and binding affinity of promising analogs, particularly 5ae, which showed strong interactions with key residues in HIV-1 PR. Overall, this study underscores the importance of flexible moieties and interaction enhancement at the S2' subsite of HIV-1 PR in developing effective DRV analogs to combat HIV and other global health issues.

Novel umami-enhancing peptides of beef M. Semimembranosus hydrolysates and interactions with the T1R1/T1R3 taste receptor

The taste mechanisms of beef umami and umami-enhancing peptides are not well understood. Therefore, novel umami and umami-enhancing peptides from beef M. semimembranosus hydrolysates were explored. Beef hydrolysates treated with Flavourzyme® showed an overall strong umami intensity compared to those treated with Alcalase®, papain, or Protamex®. The peptides were isolated via consecutive separation processes, and 31 potential umami peptides were identified. Molecular docking results showed that WGSEPIRIQ and TERGYSF had considerably low docking energies with the T1R1/T1R3 taste receptor through potential key binding sites for hydrogen bonding, including Ser48, Gly49, and Gln278 in T1R1, and Ser67, Asn68, and Arg247 in the T1R3 subunit. The taste of the identified peptides dissolved in ultrapure water was dominated by sourness. Instead, they demonstrated an umami-enhancing effect in the presence of monosodium glutamate. These results broaden our understanding of the taste mechanisms of beef umami-enhancing peptides and their potential applications as flavoring agents.

Contesting the framing of digital risk: An analysis of Australian children’s experiences

ABSTRACT Children’s social lives are mediated increasingly by digital tools, thereby making new modes of home-school-world relationships possible. In this context, children’s digital out-of-school social practices continue to be associated with risk. Children are construed as an “at risk” and vulnerable population by adults who frame children’s everyday digital social life with ambivalence. This framing is used to justify imposition of constraints in the present, with children’s digital engagement a key site of regulation. The study we report here problematises research that separates risk from children’s digital social participation. To challenge dominant views of children being vulnerable to risk, we use Actor Network Theory to provide a nuanced exposition of concepts of “digital risk” and agentic self, relevant to children’s digital social lives. From the findings of the present study, involving 62 10–11-year-old children in 3 Australian schools, we argue that “digital risk” framing of children’s social lives underplays children’s agency and voice in digital environments. Additionally, what is missing in the framing of digital risk is recognition that children utilise digital tools to ameliorate non-digital risk. In other words, risk is not only digital; and there is more to digital participation than risk.

Harringtonine metabolites: 5'-de-O-methylharringtonine and cephalotaxine, targeting spike protein and TMPRSS2 to double block membrane fusion of SARS-CoV-2 and its variants

Membrane fusion is the main pathway for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to invade host cells. Harringtonine (HT), derived from cephalotaxus fortunei Hook. f., has been recognized as an effective antagonist of SARS-CoV-2. It can directly block the active binding of spike (S) protein to host angiotensin converting enzyme 2 (ACE2), as well as hinder the enzymolysis of transmembrane serine proteases 2 (TMPRSS2). This study examined the potential of HT metabolites, 5'-de-O-methylharringtonine and cephalotaxine, as the membrane fusion inhibitors for SARS-CoV-2. 5'-De-O-methylharringtonine was synthesized and subsequently characterized by high resolution mass spectrometry and nuclear magnetic resonance to be structurally consistent, with a purity of 92.677% determined by reverse phase high performance liquid chromatography. Both 5'-de-O-methylharringtonine and cephalotaxine can specifically bind to SARS-CoV-2 S protein and TMPRSS2 using cell membrane chromatography. They can form hydrogen bonds with key sites that correlated highly with the enhanced binding affinity of SARS-CoV-2 and its variants to ACE2 or nafamostat to TMPRSS2. Moreover, 5'-de-O-methylharringtonine and cephalotaxine can inhibit pseudotyped virus entry and membrane fusion in a dose-dependent manner, with enhanced effectiveness upon elevated expression of TMPRSS2. Importantly, they displayed low cytotoxic effects on human normal cell lines. Our study suggested that 5'-de-O-methylharringtonine and cephalotaxine were of low toxicity and safety for humans as potential antagonists of SARS-CoV-2 and its variants, which deserve further validation in a biosafety level 3 facility.

The Y44 residue point mutation in CXCL14 reveals the significance of CXCL14 protein stability in anti-tumor activity

Abstract Objectives CXCL14, a member of the CXC chemokine family, plays a significant role in tumor development, progression, and metastasis, making it a potential target for cancer diagnosis and treatment. This study aims to investigate the impact of a point mutation in the (41VSRYR45) motif of CXCL14 on its anti-tumor activity. Methods Phylogenetic analysis, tertiary structure prediction, homology modeling, and cell culture experiments were employed to assess the effects of CXCL14 mutations on protein stability and anti-tumor activity. Phylogenetic analysis identified conserved regions critical for function, while computational tools predicted structural changes due to mutations. Homology modeling provided structural insights, and cell culture experiments involved transfecting HeLa cells with wild-type or mutant CXCL14 plasmids, assessing stability and anti-tumor effects. Additionally, in vivo, xenograft experiments using nude mice were conducted to evaluate the anti-tumor efficacy of CXCL14 variants. Results The conserved Y44 site within the (41VSRYR45) motif was identified as a key ubiquitination site. Mutations Y44V and Y44F enhanced CXCL14 stability, reducing HeLa cell proliferation and migration while increasing apoptosis. In vivo, tumor xenograft experiments confirmed the stronger inhibitory effect of these CXCL14 variants on tumor growth compared to the wild-type protein. Conclusion The Y44 site in CXCL14 is crucial for its stability and anti-tumor activity. Mutations Y44V and Y44F enhance CXCL14 stability and anti-tumor effects, suggesting that targeting this site could be a promising therapeutic strategy for cervical cancer.

City and Countryside in Byzantine Anatolia: Amorium

Amorium is a key site for the period AD 700-900 owing to its historical importance as the capital of the Anatolic Theme. The well preserved remains provide an exceptional opportunity to study the layout and function of the Byzantine city and its excavation sheds light on the transition, modification, and continuity of the settlement between the Late Roman and Byzantine periods. The evidence from twenty years of excavation points to the existence of a large and prosperous city during Early and Middle Byzantine times, where various trades and crafts were practised and which served as the centre of a vibrant rural community engaged in agriculture as well as animal husbandry. The destruction layers from the capture of Amorium by the Arabs in 838 create a fixed horizon and reference point for the entire archaeology and material culture of early to mid-ninth century Anatolia. Pottery and coin finds in particular provide good indicators of the level and nature of economic activity. In this study some conclusions are drawn from that material, and discussion of it is set in the context of other sites and the interpretation of finds there. The evidence from Amorium presents new insights that appear at odds with accepted views of the Byzantine world in the Early Middle Ages.

Intergenerational emotions and family history: temporal feelings and national stories

Intergenerational emotions highlight the feelings that we have for our ancestors and descendants, drawing attention to a lineage of feeling and its temporalities. This article revisits the idea of intergenerational emotions as a conceptual tool and methodological approach that is used to explore how we grapple with feelings that persist across time, and which draws attention to the importance of temporalities in shaping social structures and emotional practices. It particularly positions the family as a key site where ideas of emotion and temporality are negotiated, and so to generating intergenerational emotions as a historical and cultural construct. To illustrate this discussion, it uses the example of family history making in an exhibition by the Windrush Generation Legacy Association.

Excavating ancient pilgrimage at Nessana, Negev

Abstract The ancient site of Nessana in the south-western Negev had an important role in the logistics of early-Christian pilgrimage to the Holy Land. The arid climate, which preserved organic material, and the richness of multilingual epigraphic evidence from this region make Nessana a key site for archaeological study of the material culture of pilgrimage.