Key Route Research Articles

Effective Components and Molecular Mechanism of Biejiaruangan Capsule Against Liver Fibrosis: High-resolution Mass Spectrometry, Network Pharmacological Analysis and Experimental Verification.

Biejiaruangan capsule (BJRGC) is a commonly used traditional Chinese medicine preparation for treating oftreating liver fibrosis (LF), but its specific molecular mechanism is unclear. This study used mass spectrometry, network pharmacology and experimental verification to explore the mechanism of BJRGC against LF. Ultrahigh-performance liquid chromatography-quadrupole-exactive-orbitrap-mass spectrometry (UHPLC-Q-Exactive-Orbitrap-MS) and network pharmacology were employed to identify and screen the potential components, targets, and signaling pathways of BJRGC against LF. The interaction between the active ingredients and targets was validated using molecular docking. Finally, 5-ethynyl-2'-deoxyuridine (EDU) staining, western blotting (WB), and flow cytometry (FCM) were utilized to further verify the mechanism of BJRGC against LF. A total of 9 prototype components of BJRGC were identified in serum, most derived from iridoid glycosides and triterpenes in Gardenia jasminoides Ellis and Artemisia scoparia Waldst.et Kit. Network pharmacology predicts that medicine prototype components in serum mostly influence targets such as CDK2, CDK6, and PIK3CG, with the key route being the PI3K/AKT signaling pathway. Molecular docking showed that the major components have good binding properties with key target proteins. The experimental results showed that BJRGC could inhibit the proliferation of HSCs, induce cell cycle arrest and reduce the protein expression of CDK2, CDK6 and PIK3CG. BJRGC can inhibit the proliferation of HSCs by targeting the protein expression of CDK2, CDK6, and PIK3CG in the PI3K/AKT signaling pathway through its prototype components, such as hyperoside, tumulosic acid, and hederagenin, thereby alleviating LF disease.

Main routes and attractions of Crimea in the sphere of art tourism

This article examines the key routes and attractions of Crimea, which play a significant role in the development of art tourism on the peninsula. Crimea, with its rich historical and cultural heritage, attracts artists, writers and art lovers from all over the world. In the article, we analyze the most interesting points, such as: Bakhchisarai, Yalta, Sevastopol and Simferopol, where art galleries, museums, plein airs and cultural events are concentrated. Particular attention is paid to places of nature that inspired famous artists, as well as modern art spaces that contribute to the development of creative initiatives. During the study, we note the impact of art tourism on the economy of the region and its potential for the sustainable development of the cultural environment of Crimea. The article also includes recommendations for organizing cultural routes for tourists, taking into account the artistic context, which contributes to immersion in the unique atmosphere of Crimean art.

Student mobility in Southern Italy: An empirical analysis of preferential patterns

This paper presents an empirical analysis aimed at identifying chain migration patterns among university students from Sicily, Sardinia, and Apulia. Utilizing data from the national archive of university students enrolled in an Italian university from 2008 to 2017, we construct origin–destination matrixes and calculate residuals from a log-linear model to detect key student routes. Positive residuals between specific origin–destination pairs that persist over time can suggest significant attraction and potential chain migration. The results support a “large to large” and “small to small” city mobility model, particularly for Sicily and Sardinia. Students from Palermo, Catania, Messina, and Cagliari show strong connections with large cities such as Rome and Milan. Furthermore, results show historical links between Sardinia and Tuscany date back to the 1950s, and the enduring connections between Apulian students and universities in Emilia-Romagna, Veneto, and Lombardy have been evident since the 1970s. In Sicily, mobility patterns towards the Polytechnic of Turin began in the 1970s and persist today. These patterns underscore the influence of prior migrants on the mobility decisions of university students in Southern Italy, highlighting a potential chain migration effect.

The Spatial Geographies of Women Urban Runners

ABSTRACT Taking the northern suburbs of Melbourne, Australia, as a case study, we researched the running experiences of women through a feminist geography lens. Interviews with runners informed the design and interpretation of a spatial analysis of women’s running patterns in Strava Metro data. Our study showed how women runners are limited in where they run, and illuminated features of urban environments that can contribute to gendered limitations running women experience. Our approach took us beyond critiques of park management (e.g. lighting, toilets, trail surface), to eventually form findings about how urban form influences the spatial geographies of women runners. HIGHLIGHTS Women restrict where they run alone more than men do because of fear of being attacked by a man, avoiding isolated places in particular. Running is an activity in which women can feel at their most vulnerable in urban public space, as they are often alone, outside beyond daylight hours, and are seeking soft surfaced paths away from motor traffic, places where real-life passive surveillance is usually lower. Gendered differences in the use of urban space by runners can be observed in Strava Metro data. A key feature of women’s running route decision-making is that women will avoid and seek certain places based on how many other people they expect to be present there. Women runners benefit from urban forms which encourage high pedestrian activity, including medium-high urban density and pedestrian prioritised links to greenspaces that are separated from key traffic routes.

ВПЛИВ ВІЙНИ НА МІЖНАРОДНІ ТРАНСПОРТНІ ПЕРЕВЕЗЕННЯ УКРАЇНИ

The article found that the full-scale war in Ukraine had a strong impact on international transportation. The structure of the volume of cargo transportation and the number of transported passengers were analyzed. It was determined that there is uncertainty in the market of international freight transportation of Ukraine during the studied period. On the one hand, the war had a negative impact on the country's economy and led to a decrease in the volume of cargo transportation. On the other hand, Ukraine is an important transit country through which key logistics routes connecting Europe and Asia pass. It was established that the blocking of air transport and sea trade, with the exception of the "Grain Initiative", led to a radical change in the structure of domestic import/export and the leadership of land corridors. Global transformations in world trade caused by the war in Ukraine also affected the configuration of existing supply chains, Ukrainian exports were completely reoriented towards the EU. It is indicated that Ukraine's signing of an agreement on visa-free transport cooperation with the European Union has opened up new prospects for bilateral international road transport, freeing Ukrainian carriers from the need to obtain permits for flights to EU countries. However, there are also negative trends that affect the growth of transport services and, as a result, decrease the competitiveness of transport companies. A list of legislative and regulatory innovations and organizational measures aimed at improving international transportation is given. It is argued that a successful solution for the recovery of the country will be the integration of the Ukrainian economy into the EU economic system with the help of various European logistics and infrastructure projects. It was determined that the key factors in the normalization of transportation were: the conclusion of the "Grain Initiative" with the UN and Turkey, which allowed to unblock the ports of Great Odesa; signing the Agreement on the Liberalization of Road Freight Transportation with the EU; development of border infrastructure, in particular, increasing the capacity of existing road and railway checkpoints, as well as the opening of new ones; increasing cargo handling in the ports of the Danube port cluster.

AVIAN HAEMOSPORIDIANS IN GREATER SCAUP (AYTHYA MARILA) AND LESSER SCAUP (AYTHYA AFFINIS) FROM WISCONSIN.

Avian haemosporidians are a diverse group of protozoan parasites that infect a wide range of host species. Waterfowl are an ecologically and economically important group of hosts that have been underrepresented in studies of haemosporidians. Diving ducks have unique life history traits, and morphological, behavioral, and dietary differences separate them from more common dabbling ducks. Greater scaup (Aythya marila) and lesser scaup (Aythya affinis) are closely related diving ducks with declining population trends in North America. To better understand the diversity of haemosporidians within diving ducks and factors related to host infections in scaup, we surveyed 82 hunter-donated waterfowl from 8 species of divers, sea ducks, and dabblers from Green Bay, Wisconsin from 2019 to 2021. We used molecular detection methods and phylogenetic and statistical analyses to describe the diversity, host associations, and prevalence of haemosporidians. We detected 14 unique genetic lineages of haemosporidians, including 4 novel lineages. We identified at least 1 lineage of haemosporidian in each of the 8 host species of divers, sea ducks, and dabblers examined. Lesser scaup had more diverse haemosporidian communities than did greater scaup, but lineages showed no clustering among these hosts when incorporated in phylogenetic analyses with lineages from other Nearctic waterfowl. Female lesser scaup had the highest infection prevalence, but there was no effect of host age or year of sampling. Our findings underscore the importance of species and sex differences that could lead to a higher risk of infections. Our results also fill an important geographical sampling gap for haemosporidians along a key migratory route. Increased monitoring of haemosporidians in waterfowl could contribute to insights into parasite evolution and ecology and the conservation and management of host populations.

Перспективы международного транзита по Северному морскому пути

The Northern Sea Route (NSR) serves as a vital route for Russia, driven by both economic demands and the need for industrial development in the Arctic. The country primarily uses the NSR to supply goods to its northern regions, with the route also used by Russian companies involved in Arctic projects. As a result of economic growth in the Russian Arctic, advances in transportation technologies for navigating harsh Arctic conditions, and ongoing climate change, the NSR is expected to grow in significance as a global transport corridor, including its role in international trade. This article analyzes freight traffic and assesses the NSR’s potential for international transit. The study aims to identify promising directions for the future development of international transit along the NSR. It examines domestic and transit freight traffic dynamics over an eleven-year period, concluding that recent geopolitical events in Ukraine and the resulting anti-Russian sanctions have impacted transit traffic volumes. Key challenges to the development of NSR transit freight traffic are identified, along with their potential consequences. The study assesses the NSR’s transit capacity and outlines priority actions for unlocking its full potential. Two primary areas for future transit development are highlighted: facilitating transit traffic from China and increasing container traffic volumes, both of which warrant further exploration. The relevance of this study is underscored by the growing need to enhance NSR transit freight traffic. The scientific contribution of this research lies in its evaluation of the NSR’s transit potential using statistical analysis and in identifying strategic directions for transit development. The practical significance of the study is reflected in its recommendations, which aim to support the growth of the NSR as a key international transit route.

Examination of Turkish Culture and Customs before Islam: Outcomes and Implications

This article first investigates the activities, life, and significant role of Turkish peoples in the history of Central Asia and the Middle East before the arrival of Islam. Initially, Turks were introduced as one of the major influential groups in the Central Asian region, living a nomadic and pastoral lifestyle in the plain areas. The article secondly, explores various pre-Islamic religious beliefs and practices of the Turks, including Tengri or Tengrism (worship of natural forces), Buddhism, Manichaeism, Shamanism, and Zoroastrianism. In addition, the paper describes the Turks' strategic location with the key trade routes, Turkish customs such as the celebration of Nowruz, the nomadic lifestyle, and the wide range of handicrafts including metalwork, pottery, and weaving. The paper also slightly covers the different ethnic and historical origins of Turkish people, such as the Oghuz, Seljuks, Kipchaks, and Ottomans. Eventually, the article explains the tribal social structure of the Turks, the role of khans and traditional leaders in their societies, and the dominant feudal system before Islam.

Zero‐Bias Anti‐Ohmic Behaviour in Diradicaloid Molecular Wires

AbstractOpen‐shell materials bearing multiple spin centres provide a key route to efficient charge transport in single‐molecule electronic devices. They have narrow energy gaps, and their molecular orbitals align closely to the Fermi level of the metallic electrodes, thus allowing efficient electronic transport and higher conductance. Maintaining and stabilising multiple open‐shell states—especially in contact with metallic electrodes—is however very challenging, generally requiring a continuous chemical or electrochemical potential to avoid self‐immolation of the open‐shell character. To overcome this issue, we designed, synthesised, and measured the conductance of a series of bis(indeno) fused acenes, where stability is imparted by a close‐shell quinoidal conformation in resonance with the diradical electronic configuration. We show here that these compounds have anti‐ohmic behaviour, with conductance increasing with increasing molecular length, at an unprecedented rate and across the entire bias window ( ). Density Functional Theory (DFT) calculations support our findings, showing the rapidly narrowing HOMO–LUMO gap, unique to these diradicaloid structures, is responsible for the observed behaviour. Our results provide a framework for achieving efficient transport in neutral compounds and demonstrate the promise that diradicaloid materials have in single‐molecule electronics, owing to their great stability and unique electronic structure.

Development and Application of an Advanced Automatic Identification System (AIS)-Based Ship Trajectory Extraction Framework for Maritime Traffic Analysis

This study addresses the challenges of maritime traffic management in the western waters of Taiwan, a region characterized by substantial commercial shipping activity and ongoing environmental development. Using 2023 Automatic Identification System (AIS) data, this study develops a robust feature extraction framework involving data cleaning, anomaly trajectory point detection, trajectory compression, and advanced processing techniques. Dynamic Time Warping (DTW) and the Hierarchical Density-Based Spatial Clustering of Applications with Noise (HDBSCAN) algorithms are applied to cluster the trajectory data, revealing 16 distinct maritime traffic patterns, key navigation routes, and intersections. The findings provide fresh perspectives on analyzing maritime traffic, identifying high-risk areas, and informing safety and spatial planning. In practical applications, the results help navigators optimize route planning, improve resource allocation for maritime authorities, and inform the development of infrastructure and navigational aids. Furthermore, these outcomes are essential for detecting abnormal ship behavior, and they highlight the potential of route extraction in maritime surveillance.

An application of gravity models to freight data in Malaysia

Background: Freight transport services and international trade are among important factors in pushing economies’ transition forward. Malaysia’s strategic location along key maritime routes positions it as a vital hub for international shipping and logistics, facilitating efficient trade between Asia, Europe and the Middle East.Objectives: Despite extensive global studies on freight and trade, little specific research exists from Malaysia’s perspective. Thus, this study aims to evaluate how factors such as economic size of trade partners and distance impact Malaysia’s freight transport in international trade, using the gravity model, a tool for analysing bilateral trade flows.Method: Three types of gravity models are employed to assess the impact of economic scale, infrastructure and demographic factors on freight transport. Then the validity of these relationships is further evaluated.Results: The random effect model (REM) is found to be the best fitted model. The study identifies infrastructure and economic scale as pivotal factors influencing both freight export and import. The result showed the volume of container handled in ports is the main determinant in both export and import of freight, whereas air freight has significant impact to boost the export of freight. Distance is statistically significant, however, with marginally small impact.Conclusion: Based on the analysis, we can conclude that the robust port infrastructure and efficient logistical facilities are important in reducing freight costs and enhancing Malaysia’s international trade competitiveness.Contribution: This study mainly contributes on providing empirical evidence and in-depth insight on Malaysia freight transport services through the application of gravity models.

A governance framework for facilitating cross-agency data sharing

Linking data across local authorities and health boards has the potential to reduce inequalities and improve outcomes. However, cross-agency data sharing leads to complexities around data ownership, legal conditions for processing, organisational controls and risk assessments to ensure privacy and confidentiality of subjects whilst facilitating research and service improvements. We share a governance framework for cross-agency data sharing between a local authority and regional health board in Scotland. A cross-sector Child Neglect working group was established with representation from public sector agencies, the NHS, University researchers and a Trusted Research Environment to support research on children’s mental health prescribing and secondary specialist services provision and linking to the Aberdeen City Council Child Protection Register. Lessons learned were captured throughout the governance, data transfer and data linkage phases. Communication is the most important driver, with key individuals, deputies and routes of escalation agreed and involved throughout for clarity of roles and expectation setting. Key lessons learned include requiring input from all parties at the project initiation stage, particularly IG teams, including agreements on necessary templates. Knowledge exchange is required between the research team, data owners and data processors to ensure research protocol requirements are correctly understood and data transfer is supported. If possible, internal and/or external validation of data should also be performed before data is linked. Cross-agency data sharing is a vital means to understanding and improving outcomes for individuals and populations. Critical to this is establishing an action plan and formalised project process to achieve outcomes.

TrafPS: A shapley-based visual analytics approach to interpret traffic

Recent achievements in deep learning (DL) have demonstrated its potential in predicting traffic flows. Such predictions are beneficial for understanding the situation and making traffic control decisions. However, most state-of-the-art DL models are considered “black boxes” with little to no transparency of the underlying mechanisms for end users. Some previous studies attempted to “open the black box” and increase the interpretability of generated predictions. However, handling complex models on large-scale spatiotemporal data and discovering salient spatial and temporal patterns that significantly influence traffic flow remain challenging. To overcome these challenges, we present TrafPS, a visual analytics approach for interpreting traffic prediction outcomes to support decision-making in traffic management and urban planning. The measurements region SHAP and trajectory SHAP are proposed to quantify the impact of flow patterns on urban traffic at different levels. Based on the task requirements from domain experts, we employed an interactive visual interface for the multi-aspect exploration and analysis of significant flow patterns. Two real-world case studies demonstrate the effectiveness of TrafPS in identifying key routes and providing decision-making support for urban planning.

Assessing Per- and Polyfluoroalkyl Substances in Fish Fillet Using Non-Targeted Analyses.

Per- and polyfluoroalkyl substances (PFAS) are a class of thousands of man-made chemicals that are persistent and highly stable in the environment. Fish consumption has been identified as a key route of PFAS exposure for humans. However, routine fish monitoring targets only a handful of PFAS, and non-targeted analyses have largely only evaluated fish from heavily PFAS-impacted waters. Here, we evaluated PFAS in fish fillets from recreational and drinking water sources in central North Carolina to assess whether PFAS are present in these fillets that would not be detected by conventional targeted methods. We used liquid chromatography, ion mobility spectrometry, and mass spectrometry (LC-IMS-MS) to collect full scan feature data, performed suspect screening using an in-house library of 100 PFAS for high confidence feature identification, searched for additional PFAS features using non-targeted data analyses, and quantified perfluorooctanesulfonic acid (PFOS) in the fillet samples. A total of 36 PFAS were detected in the fish fillets, including 19 that would not be detected using common targeted methods, with a minimum of 6 and a maximum of 22 in individual fish. Median fillet PFOS levels were concerningly high at 11.6 to 42.3 ppb, and no significant correlation between PFOS levels and number of PFAS per fish was observed. Future PFAS monitoring in this region should target more of these 36 PFAS, and other regions not considered heavily PFAS contaminated should consider incorporating non-targeted analyses into ongoing fish monitoring studies.

Nrf2-mediated ferroptosis inhibition: a novel approach for managing inflammatory diseases.

Inflammatory diseases, including psoriasis, atherosclerosis, rheumatoid arthritis, and ulcerative colitis, are characterized by persistent inflammation. Moreover, the existing treatments for inflammatory diseases only provide temporary relief by controlling symptoms, and treatments of unstable and expensive. Therefore, new therapeutic solutions are urgently needed to address the underlying causes or symptoms of inflammatory diseases. Inflammation frequently coincides with a high level of (reactive oxygen species) ROS activation, serving as a fundamental element in numerous physiological and pathological phenotypes that can result in serious harm to the organism. Given its pivotal role in inflammation, oxidative stress, and ferroptosis, ROS represents a focal node for investigating the (nuclear factor E2-related factor 2) Nrf2 pathway and ferroptosis, both of which are intricately linked to ROS. Ferroptosis is mainly triggered by oxidative stress and involves iron-dependent lipid peroxidation. The transcription factor Nrf2 targets several genes within the ferroptosis pathway. Recent studies have shown that Nrf2 plays a significant role in three key ferroptosis-related routes, including the synthesis and metabolism of glutathione/glutathione peroxidase 4, iron metabolism, and lipid processes. As a result, ferroptosis-related treatments for inflammatory diseases have attracted much attention. Moreover, drugs targeting Nrf2 can be used to manage inflammatory conditions. This review aimed to assess ferroptosis regulation mechanism and the role of Nrf2 in ferroptosis inhibition. Therefore, this review article may provide the basis for more research regarding the treatment of inflammatory diseases through Nrf2-inhibited ferroptosis.

Boosting electrocatalytic ammonia synthesis from nitrate by asymmetric chemical potential activated interfacial electric fields

The electrocatalytic nitrate reduction reaction (NO3− RR) has immense potential to alleviate the problem of groundwater pollution and may also become a key route for the environmentally benign production of ammonia (NH3) products. Here, the unique effects of interfacial electric fields arising from asymmetric chemical potentials and local defects were integrated into the binary Bi2S3-Bi2O3 sublattices for enhancing electrocatalytic nitrate reduction reactions. The obtained binary system showed a superior Faraday efficiency (FE) for ammonia production of 94 % and an NH3 yield rate of 89.83 mg gcat-1h−1 at −0.4 V vs. RHE. Systematic experimental and computational results confirmed that the concerted interplay between interfacial electric fields and local defects not only promoted the accumulation and adsorption of NO3–, but also contributed to the destabilization of *NO and the subsequent deoxygenation hydrogenation reaction. This work will stimulate future designs of heterostructured catalysts for efficient electrocatalytic nitrate reduction reactions.

High-speed shearing: An ultra-fast internal energy accumulation method for nucleation of SnZnO in photocatalysis

Zinc oxide (ZnO) nanoparticles and their derivatives, appreciated for their cost-effectiveness, simplicity in synthesis, and versatile applications, have garnered considerable attention. However, unraveling the nucleation and growth mechanisms of ZnO nanoparticles remains a formidable challenge. By comparing the magnetic stirring synthesis (MSS) and high-speed shearing (HSS) methods, we conducted time-dependent calculations of pressure and velocity at various positions in the solution. Under the powerful shearing force, which is the key route to accumulate internal energy (Ein), molecules could achieve at most 174 eV Ein in HSS instead of 0.81 eV Ein in MSS. Molecules could obtain enough energy for nucleation in just 1.03 μs. HSS, utilizing a high-speed rotor for robust shear forces, not only modifies nucleation patterns but also facilitates effective mixing in multi-element systems. SnxZn1−xO1+x nanoparticles prepared through HSS display uniform size, markedly reduced agglomeration, homogeneous element distribution, and a more pronounced bandgap change, causing a decrease in photocatalytic activity to the original 11.2 %.

Zero-Bias Anti-Ohmic Behaviour in Diradicaloid Molecular Wires.

Open-shell materials bearing multiple spin centres provide a key route to efficient charge transport in single-molecule electronic devices. They have narrow energy gaps, and their molecular orbitals align closely to the Fermi level of the metallic electrodes, thus allowing efficient electronic transport and higher conductance. Maintaining and stabilising multiple open-shell states-especially in contact with metallic electrodes-is however very challenging, generally requiring a continuous chemical or electrochemical potential to avoid self-immolation of the open-shell character. To overcome this issue, we designed, synthesised, and measured the conductance of a series of bis(indeno) fused acenes, where stability is imparted by a close-shell quinoidal conformation in resonance with the diradical electronic configuration. We show here that these compounds have anti-ohmic behaviour, with conductance increasing with increasing molecular length, at an unprecedented rate and across the entire bias window ( ). Density Functional Theory (DFT) calculations support our findings, showing the rapidly narrowing HOMO-LUMO gap, unique to these diradicaloid structures, is responsible for the observed behaviour. Our results provide a framework for achieving efficient transport in neutral compounds and demonstrate the promise that diradicaloid materials have in single-molecule electronics, owing to their great stability and unique electronic structure.

The Russian Revolution and photography: The tragic paradoxes of canonisation

Bourdieu (et al.’s) Photography contends that it is the fate of photography to remain for ever ‘a middlebrow art’. This is partly because its technological character is held to be inimical to canonisation as a high art-form, but also because it lacks a class willing to invest time in its reception. Here, I argue, Bourdieu has been proved wrong: photography has now been consecrated, including, ironically, his own photography. Support for this claim is drawn from the field of art in Russia. The Russian Revolution and the Constructivist movement brought into the public gaze artists such as Rodchenko, El Lissitzky and Valentina Kulagina, who saw photography as one of a series of genres that might renovate the social order and transforms the public’s habitual perception. Photography became central in the movement to replace Academic art and thus a key route to photography’s consecration. However, as Benjamin pointed out there is a danger of decontextualising and ‘eternalising’ the photographs or photomontages that make the transition into the art museum. They may remain neither the art of the community from which they emerged, nor be recognised as an expressive resource for later dominated groups; instead such canonised works may become a status adornment of those with cultural capital.

Complete 2,4,6-trichlorophenol degradation by anaerobic sludge acclimated with 4-chlorophenol: Synergetic effect of nZVI@BMPC and sodium lactate as an external nutrient

Ball-milled plastic char supported nano zero-valent iron (nZVI@BMPC) and their application combined with anaerobic sludge for microbial dechlorination of 2,4,6-trichlorophenol (2,4,6-TCP) were investigated. The XRD and FTIR analysis proved composition of zero valent states of iron, and the BET and SEM analysis showed that nZVI was uniformly distributed on the surface of BMPC. Successive addition of 1000 mg/L sodium lactate and nZVI@BMPC enhanced the acclamation of anaerobic sludge and resulted in the degradation of 4-CP within 80 days. The acclimated consortium with nZVI@BMPC completely degraded 2,4,6-TCP into CH4 and CO2, and the key dechlorination route was through 4-CP dechlorinaion and mineralization. The degradation rate of 2,4,6-TCP with nZVI@BMPC was 0.22/d, greater than that without nZVI@BMPC. The dechlorination efficiency was enhanced in the Fe2+/Fe3+ system controlled by nZVI@BMPC and iron-reducing bacteria. Metagenomic analysis result showed that the dominant de-chlorinators were Chloroflexi sp., Desulfovibrio, and Pseudomonas, which could directly degrade 2,4,6-TCP to 4-CP, especially, Chloroflexi bacterium could concurrently be used to mineralize 4-CP. The relative abundance of the functional genes cprA, acoA, acoB, and tfdB increased significantly in the presence of the nZVI@BMPC. This study provides a new strategy can be a good alternative for possible application in groundwater remediation.