AbstractIn conjunction with the advancement of digital healthcare, the field of wearable biosensors has experienced rapid growth in recent years and is projected to expand further in the coming years. As wearable biosensors enter their next phase, emphasis is increasingly placed on developing comfortable, breathable, washable, and lightweight intelligent fibers and textiles as key components. This review examines the contributions of both natural and synthetic fibers and textiles to wearable biosensors. The structure and preparation process of intelligent fibers and textiles are systematically elucidated, including the development of the conductive layer. Additionally, key micro–nano technologies that have emerged in this domain are highlighted such as built‐in power supplies, wireless data transmission, precise data analysis driven by artificial intelligence, and machine learning. Furthermore, advanced functionalities integrated into recent biosensing systems, such as heat management, real‐time displays, and human–machine interaction are summarized. Moreover, the applications of fiber‐based wearable biosensors for monitoring biophysical and biochemical signals are presented. Finally, the challenges faced by the community working on wearable sensors based on intelligent fibers and textiles are discussed alongside promising future directions.