Citrus Aurantifolia Research Articles

Comparative Study on Mechanic Workshop Polluted Soil Treated With Lime (Citrus Aurantifolia) Juice, Powder and Inorganic Lime Fertilizer

Comparative Study on Mechanic Workshop Polluted Soil Treated With Lime (Citrus Aurantifolia) Juice, Powder and Inorganic Lime Fertilizer

Effect of Gibberellic acid on seed germination and seedling vigour of Indi’s Kagzi Lime [Citrus aurantifolia (Christmas) Swingle

Effect of Gibberellic acid on seed germination and seedling vigour of Indi’s Kagzi Lime [Citrus aurantifolia (Christmas) Swingle

Hepatoprotective Properties of Citrus aurantifolia Juice and Carica papaya Seed Extract Mixture against Carbon Tetrachloride Induced Liver Injury in Albino Rats

Hepatoprotective Properties of Citrus aurantifolia Juice and Carica papaya Seed Extract Mixture against Carbon Tetrachloride Induced Liver Injury in Albino Rats

Phytochemical Screening and Anti – oxidant Activities of the Roots and Leaves of Citrus Aurantifolia (Lime orange) grown in Imane, Olamaboro, Kogi, Nigeria

Traditional doctors have been utilizing the advantages of the potency of natural plants in the treatment and prevention of several ailments for decades without them or their patience knowing the active ingredients responsible for the potency. Hence the need to evaluate and understand the medicinal bio – active substances present in plants. This work analyses the phytochemical substances and the anti – oxidant activities of the roots and leaves of citrus aurantifolia. The plant parts were collected, dried, pulverized and extracted in absolute ethanol. The extracts collected were analyzed for bio – active substances and antioxidant activities. The results show the presence of alkaloids, carbohydrates, glycosides, saponins, phenols, tannins, flavonoids, protein and amino acid in both the roots and leaves of citrus aurantifolia. The KMnO4 scavenging effects of the plant parts show them to displayed effective potentials as anti – oxidants as the % KMnO4 radical scavenging competes favorably; (root – 85.60% and leaves – 89.88%) with the reference ascorbic acid (83.82%). This indicate that the plant parts can scavenge free radical from the body. These finding denotes that the root and leave of citrus aurantifolia can be a good source of therapeutic for health management.

Relationships between Bacterial Zone of Inhibition and In-Situ Characteristics in Citrus aurantifolia Fruit Juice: A Linear Regression Perspective

Relationships between Bacterial Zone of Inhibition and In-Situ Characteristics in Citrus aurantifolia Fruit Juice: A Linear Regression Perspective

Métodos para estimar el nivel de poliembrionía en semillas de lima mexicana y limón italiano

Polyembryony is a common trait in most citrus varieties. The level of polyembryony has the potential to be used as a descriptor in the characterization of varieties or to select parents in conventional hybridization work. The aim of this research was to find an efficient methodology to detect, count and estimate the potential number of embryos per seed, the percentage of polyembryonic seeds and the level of polyembryony in the varieties ‘Colimex’ and ‘Lise’ of Mexican lime, and the variety ‘Rosenberger’ of true lemon. Four methodologies were evaluated: 1) direct counting of embryos in mature seeds (DCEMS), 2) direct counting of embryos in immature seeds (DCEIS), 3) counting of seedlings derived from mature seeds germinated in vitro (CSMSGV) and 4) counting of seedlings derived from mature seeds germinated in substrate (CSMSGS). The highest averages of embryos per seed and percentage of polyembryonic seeds were recorded with the DCEMS and CSMSGV methods (P ≤ 0.01), so it was concluded that they are adequate to estimate these variables in the genotypes studied. The varieties ‘Colimex’ and ‘Lise’ had 80.65 and 78.27 %, respectively, of seeds with two or more embryos, resulting in a high level of polyembryony, while ‘Rosenberger’, with 18.37 %, was slightly polyembryonic (P ≤ 0.01).

The effect of lime leaf extract (Citrus aurantifolia) through drinking water on the chemical and physical quality of broiler meat

This study aims to investigate the impact of supplementing lime leaf extract (EDJ) in broiler chickens' drinking water on both the chemical and physical qualities of their meat. A total of 128 New Lohmann MB 202 strain broiler roosters were subjected to the same basal feed regimen but received distinct drinking water treatments over a 35-day rearing period. The drinking water treatments included plain water without additives (negative control; T0), water supplemented with 50 ppm Tetracycline antibiotics (positive control; T1), water enriched with 15 ml/liter EDJ (T2), and water infused with 30 ml/liter EDJ (T3). The basal diet consisted of corn and soybean flour, with a crude protein content of 22.01% and a metabolic energy level of 3113.12 kcal/kg. Feed and drinking water are provided ad libitum during the rearing period. The parameters observed in this study were the chemical and physical quality of the meat, which included variables: moisture content, ash content, protein content, fat content, pH value, water holding capacity, cooking loss, and tenderness. The data obtained were then analyzed for variance (ANOVA) using a one-way Complete Randomized Design based on a P value of less than 5%. The findings of the study indicated that the supplementation of EDJ through drinking water did not produce significant alterations in water content, protein content, fat content, cooking loss, or tenderness of the broiler meat. However, a notable outcome was observed: the addition of 30 ml/liter of EDJ to the drinking water led to a substantial increase in water holding capacity (P<0.05) while simultaneously reducing the meat's pH value (P<0.05). These results suggest that EDJ supplementation has the potential to enhance the physical quality of broiler meat, primarily by increasing water retention and decreasing pH values, which can contribute to improve meat texture and juiciness.

Phytochemical and antibacterial potential of lime (Citrus aurantifolia) and lemon (Citrus limon) juices as bioactivators of charcoal

Citrus juices are widely consumed because of their many health benefits and antimicrobial properties. Biological activators are preferred to the conventional chemical activators for safety and less health hazards thus the need to search for available indigenous biological materials for charcoal activation. Hence, the study on the potentials of lime (Citrus aurantifolia) and lemon (Citrus limon) juices as biological activators for charcoal. Freshly harvested lime and lemon juice were extracted using juice extractor and sterilized through filtration with membrane filter (0.45µl pore size). The phytochemical of the juices was determined and results revealed the presence of (carbohydrates, flavonoids, cardiac glycosides, saponins, terpenoids, ketose(fructose) and reducing sugars). The lime and lemon juices were subjected to antibacterial screening by agar well diffusion method against the test bacterial isolates (Staphylococcus aureus and Salmonella enterica subsp. entericaserovar) at 75%, 50%,25% and 12.5% concentrations respectively. The lime and lemon juice inhibition effects decreased with decrease in concentration. However, lime juice demonstrated higher inhibition across all concentrations in both test organisms with zones of inhibition (12-30mm) in Salmonella and (10-28 mm) in S. aureus while lemon juice demonstrated a low antibacterial activity across the different concentrations with zones of inhibition ranging from (12-17mm) in Salmonella and (9-24mm) in S. aureus. The phytochemical and antibacterial activities of lime and lemon juice revealed their potential utilization as bio activators.

Population dynamics of key lime pest insects in two agroecological zones of Manabí, Ecuador

El cultivo de limón es muy importante en la economía mundial, su producción está limitada por el ataque de plagas. El objetivo de la investigación es estudiar la dinámica poblacional de los insectos plaga del limón sutil en dos zonas agro ecológicas de Manabí, Ecuador. El estudio se realizó entre agosto/2018 y julio/2019, en fincas, una para la localidad de Colón (Longitud: O80º24'47'', Latitud: S1º6'47'') y para la localidad de Riochico (Longitud: O80º24'59'', Latitud: S0º59'42''). Se determinó la dinámica poblacional de insectos plaga por un año en una finca productora de limón de ocho años de edad, para los distritos de Colón y Riochico donde se realizaron evaluaciones una vez al mes. La metodología empleada fue seleccionar 10 árboles al azar, de cada árbol se evaluó un número determinado de hojas, esto permitió establecer poblaciones de cada una de estas plagas (Castaño, 1996; Cañarte 2001). Se estudió la población de los insectos plaga Phyllocnistis citrella, Aleurothrixus floccosus, Toxoptera aurantii y factores climatológicos (temperatura, humedad relativa y precipitación) de las zonas analizadas. Se concluyó que las mayores poblaciones de los fitófagos Toxoptera aurantii y Aleurothrixus floccosus se concentraron entre los meses de junio a diciembre correspondientes a la estación seca, con Phyllocnistis citrella los meses de mayor captura comprendieron entre enero y mayo, durante la estación lluviosa. Las condiciones de baja humedad relativa, escasa precipitación y temperatura favorecieron el desarrollo de A. floccosus y T. aurantii. Condiciones de alta humedad relativa, abundante precipitación y temperatura cálida aportaron al desarrollo de P. citrella. Los estados vegetativos están relacionados con la mayor sensibilidad del cultivo a estas plagas, contribuyendo a determinar los momentos más oportunos, para realizar tratamientos y poder controlar los daños.

Enhancing photosynthesis and root development for better fruit quality, aroma, and lessening of radioactive materials in key lime (Citrus aurantifolia) using Trichoderma harzianum and Bacillus thuringiensis

The present study was conducted to investigate the effects of Trichoderma harzianum and Bacillus thuringiensis alone or with gradual levels of NPK on photosynthesis, growth, fruit quality, aroma improvement and reduced radionuclides of key lime fruits. The lemon seedlings were treated with (T0) without fertilizers as control, (T1) 100g of NPK at 100%, (T2) 5 g of Trichoderma. harzianum at 50% + 50 g of NPK at 50%, (T3) 5 g of Bacillus thuringiensis at 50% + 50 g of NPK at 50 %, (T4) 7.5 g of Trichoderma harzianum at 75% + 25 g of NPK at 25 %, (T5) 7.5 g of Bacillus thuringiensis at 75% + 25 g of NPK at 25 %, (T6) 10 g of Trichoderma harzianum at 100 % and (T7)10 g of Bacillus thuringiensis at 100 %. The results showed that T2 increased net photosynthetic rate, stomatal conductance, transpiration rate, internal CO2 concentration, fresh and dry root biomass by 209%, 74%, 56%, 376%, 69.4% and 71.6%, while, T5 increased root volume, root length, and root tip number by 27.1%, 167%, and 67%, respectively over the control trees. The microbial treatments developed cortex, vascular cylinder and tracheal elements of the root. Fruit number, length, diameter, weight, pulp thickness, pulp/peel ratio, juice, total soluble solids (TSS), pigment contents and antioxidant activity increased significantly in the T2 treatment. Vitamin C, total phenols, total flavonoids, and total sugar content increased by 1.59-, 1.66-, 1.44- and 2.07- fold in T5 treated fruits compared to the control. The two microbes increased volatile compounds and decreased radionucleotides in the fruit, moreover, 27 identified and 2 (two) unmatched volatile compounds were identified by GCMS analysis. It is concluded that T. harzianum and B. thuringiensis with 25–50 g NPK treatments improved photosynthesis, root structure, fruit growth, fruit quality, aroma and lessened radionuclides in key lime fruits.

Characterization of Neglected and Underutilized Fruits in the Philippines

Twelve previously unreported fruit species native to the Philippines were examined for their unique characteristics. ‘Bayag-usa’ (Voacanga globosa) is a 60.8±3.7 g dark gray, reniform fruit. Red mangosteen (Garcinia hombroniana) is a bright red, globose fruit weighing 45.2±7.77 g, with 18±2 °Brix total soluble solids (TSS). The false mangosteen (Garcinia xanthochymus) is light yellow, heart-shaped and weighs 151±36.46 g, with TSS at 16±0.89 °Brix. Yellow mabolo (Diospyros blancoi) is canary yellow, depressed ovate, weighing 273.33±87.9 g, with edible portion (EP) of 78.44±1.2%. ‘Kolis’ (Memecylon ovatum) is round, dark purple, weighing 0.73±0.02 g, with TSS at 13±1.41 °Brix and an EP of 84.2±7.59%. Raspberry Bush (Randia formosa) is a 2.03±0.18 g yellow, tubular fruit. ‘Gabiroba’ (Compomanesia xantocarpa) is round, yellow, weighs 11.95±1.68 g, has 18±1.08 oBrix TSS, and is sub-acid with a Titratable Acidity (TA) of 0.13±0.75 meq/10 mL juice. ‘Tugos’ (Alpinia galanga) is globose, yellow-brownish and weighs 1.55±0.50 g. ‘Gapas-gapas’ (Citrus hystrix) is sour, weighing 66.18±2.89 g, with a TA of 1.06±0.23 meq/10 mL juice. ‘Bisbis’ (Citrus aurantifolia) is green, weighing 36.59±2.81 g, with a TA of 0.85±0.26 meq/10 mL juice. Additionally, ‘Kolisom’ (Citrus hystrix var. micrantha) is green, sour, weighing 65.78±2.7 g, with a TA of 1.59±0.84 meq/10 mL juice, and ‘Kolong-kolong’ (Citrus hystrix var. boholensis) is also green, weighing 64±1.7 g, with a TA of 1.1±0.7 meq/10 mL juice. The study also explored the pharmacological and ethnobotanical uses of these diverse species.

Enhancing Shelf-Life Quality of Mexican Lime (Citrus Aurantifolia) Fruit Using Gelatin Edible Coating Incorporated with Pomegranate Seed Oil

Enhancing Shelf-Life Quality of Mexican Lime (Citrus Aurantifolia) Fruit Using Gelatin Edible Coating Incorporated with Pomegranate Seed Oil

Therapeutic efficacy of Citrus aurantifolia (lime) juice in experimental Eimeria tenella-infected broiler chickens.

The purpose of the present experiment was to explore the therapeutic effect of the crude fruit juice of Citrus aurantifolia (CAJ) on Eimeria tenella disorder in chickens. One hundred twenty 3-week-old Ross 308 broilers of equal sexes were assigned to six experimental groups of 20 birds each. Groups A, B, C, D, and E were experimentally infected with 20,000 sporulated Eimeria tenella oocysts. Broilers in groups A, B, and C were infected and allocated to three treatment-graded doses of C. aurantifolia fruit juice (20, 10, and 5 mL/kg body weight, respectively) which were administered orally for 7 consecutive days of the trial. Group D was infected and treated with a reference drug, Amprolium 1.5 g/L of drinking water (positive control), group E served as infected-untreated control, and group F was uninfected and non-treated (negative control). Oocysts per gram of feces were counted using the McMaster counting device, weight gain was calculated, and blood samples from each experimental group were collected on days 0, 7, 14, 21, and 28 post-infection for hematological evaluation. Results revealed that medication of broilers with C. aurantifolia fruit juice dose-dependently increased body weight, improved cecal lesion, decreased the E. tenella oocyst production rate, and significantly (P<0.05) increased the PCV of the infected broilers. C. aurantifolia provided valuable therapeutic effects against E. tenella-induced coccidiosis in broiler chickens. The plant fruit juice should be further validated to explore the vital compounds responsible for the anticoccidial efficacy.

Optimization of Citrus aurantifolia peel extract encapsulation in alginate-gelatin hydrogel microbeads for antibacterial wound dressing applications

The extract of Citrus aurantifolia peel has been encapsulated into alginate-gelatin-based microbeads using an external ionotropic gelation method to apply antibacterial wound dressings. Such variables, like the alginate-gelatin ratio, extract quantity, and CaCl2 cross-linkers, were optimized to produce a good yield, well-distributed size, and encapsulation efficiency. Antibacterial efficacy was tested against both S. aureus and E. coli. Optimal microbead characteristics were obtained at alginate:gelatin concentration ratio of 1.75 %:0.25 % (w/v), with 15 mg of extract and a 2 % CaCl2 cross-linker. These conditions resulted in the microbeads with 82.94 ± 1.056 % yield, particle sizes between 1.080–1.244 µm, and encapsulation efficiency of 79.127 ± 0.186 %. The microbeads exhibited an almost spherical morphology with a slightly rough surface. The extract release mechanism out of the microbeads was governed by both diffusion and erosion of the shells following the Korsmeyer-Peppas kinetic model (k = 1.014 ± 0.007/min). Upon immobilizing the microbeads on gauze, an add-on percentage of 73.98 % was observed. Moreover, the gauze-immobilized microbeads inhibited S. aureus and E. coli growth with inhibition zones of 11±1.48 and 12.56±4.89 mm, respectively. In conclusion, the antibacterial alginate-gelatin-based microbeads have demonstrated optimal characteristics and exhibited effective inhibition against S. aureus and E. coli, showcasing their potential as antibacterial wound dressings.

Effect of fertilization of Seaweed extracts and CuSO4 on some vegetative growth indicators of Citrus limon L. grafted seedlings on rootstock aurantifolia

Citrus aurantifolia rootstock seedlings were treated with Seaweed extracts and copper sulfate. Seaweed extracts were added as a ground feed during the growing season at four levels (0, 10, 20, and 30 ml. seedling-1) and copper sulfate sprayed at four concentrations (15,10,5, 0 ml. L-1) alone or in combination with the control (watering with water only) during the growing seasons 2020-2021. The results showed that the treatment of seedlings grafted on rootstock aurantifolia with Seaweed extracts and spraying with copper sulfate affected (height, stem diameter, area Leaf, root length, and chlorophyll content of leaves (the results also showed that the addition of Seaweed extracts at a concentration of 30 ml overlapping with copper sulfate spraying at a concentration of 15 ml. L-1 is recommended to improve the vegetative, root and nutritional growth of lemon citrus seedlings.

Exploring the Antibacterial Potency of Citrus Fruit Peel Extracts Using Various Solvents

The study evaluates the inhibitory effect of different solvent-extracted citrus peel extracts (Citrus lemon, Citrus sinensis, Citrus maxima, Citrus reticulata and Citrus aurantiifolia) on the growth of various bacterial strains (Klebsiella. pneumonia, Staphylococcus. aureus, Pseudomonas aeruginosa, and Escherichia coli). Citrus lemon extract demonstrated the most significant inhibition with methanol and acetone showing the highest zones of inhibition. Citrus sinensis extract showed antibacterial activity, with the choice of solvent influencing the effectiveness. Methanol and acetone generally performed well. Citrus maxima extract demonstrated antibacterial activity, with methanol and butanol being effective solvents. Citrus reticulata extract's effectiveness varied depending on the solvent and bacterial strain tested. Ethanol and butanol were more effective solvents for inhibiting the growth of some bacteria, while acetone and methanol showed limited effectiveness. The Citrus aurentiifolia peel extract exhibited varying degrees of antibacterial activity against the tested bacteria. The effectiveness of the extract depended on the solvent used and the type of bacteria. For some bacteria, such as E. coli and S. aureus, the extract showed significant inhibitory effects, particularly when using Ethanol as the solvent. Further research and optimization may be necessary to enhance the antibacterial properties of Citrus aurentiifolia peel extract.

Determination of Vitamin C Levels in Limes (Citrus aurantifolia Swingle) and Lemon (Citrus limon (L.) Burm. f.) Using the UV Vis Spectrophotometric Method

Vitamin C or ascorbic acid is one of the vitamins needed by the body which functions to help the body's metabolic processes and plays a role in the formation of intercellular collagen. The source of vitamin C in food mostly comes from fresh vegetables and fruit. Limes and lemons are plants that contain vitamin C and have many benefits for the body. This research The aim was to determine the levels of vitamin C in limes (Citrus aurantifolia) and lemon (Citrus Limon) are planted in the area Situmandala Rancah District using UV-Vis Spectrophotometry at a wavelength of 400-600 nm. The samples for this research were limes and lemons. The results of this research showed that the vitamin C level in limes was 0.218% and in lemons it was 0.816%.

Effect Of Microwave In The Green Synthesis Of Colloidal Bismuth Nanoparticles

In this study, bismuth nanoparticles were synthesized and prepared using citrus aurantifolia green synthesis method. The effect of microwave heating was examined. Citrus aurantifolia was used to reduce metal ions and nanoparticle formation due to the presence of various biomolecules needed. microwave was chosen because it can accelerate and form more stable nanoparticles. Bismuth nanoparticles were successfully synthesized with UV-Vis wavelength at 276 nm and nanoparticle average size is 4.66 nm. This result also shows a better TEM result image when with microwave because there is no impurity from bioreductor.

Analysis of Nutritional Components, Antioxidant Activity and Antimicrobial Activity of Citrus Aurantiifolia (Christm.) Swingle of Assam, India

Citrus aurantiifolia (Christm.) Swingle, mostly known as the Key lime or Mexican lime. In Assam, it is known as ‘Gol Nemu’, which is a small, evergreen citrus tree having significant place in the botanical geography of Assam, India. The present study is concentrated on the analysis of the nutritional components, antioxidant activity, and antimicrobial properties of Citrus aurantiifolia (Christm.) Swingle, cultivated in the region of Kamrup District, Assam, India. The results of this research provide scientific understanding of Citrus aurantiifolia as an abundant source of nutrients like soluble carbohydrate, soluble protein, free fatty acids, vitamin C, along with potent antioxidant and antimicrobial properties. The illumination of this study could have implications for its implementation in both nutraceutical and pharmaceutical industries, as well as in encouraging its consumption for enhanced well-being.

Inventory of the Citrus in Barimbun Village, Tabalong District

Indonesia is rich in flora diversity, especially fruit plants. One of fruit plants that is familiar to Indonesian people is the orange tree. Orange trees are annual plants originating from the Asian continent, especially India to China. In Indonesia, orange plants are diverse and have unique characteristics in the form of aroma, color and taste that are different from other fruits. The aim of this research is to describe the diversity of citrus plants (genus Citrus) in Barimbun Village, Tabalong. This research uses the line transect method. The research results found 7 types of oranges, namely, Citrus microcarpa, Citrus jambhiri, Citrus aurantifolia, Citrus gandis, Citrus hystrix, Citrus sinensis, and Citrus limon. Moreover, the abiotic factors give good value which means a suitable habitat for oranges. From this study, it may conclude that Barimbun Village, Tabalong District is a suitable habitat for orange plant because this area has enough amount of rainfall and stabil temperature.