The growing technology investment in driverless cars, warehouse automation, human-service robots, and artificial intelligence applications in our daily lives is inspiring. However, the majority of this effort assumes a structured environment, which leaves significant, unsolved problems for autonomous robots operating in unstructured environments. Beginning in 2010, the U.S. Combat Capabilities Development Command, Army Research Laboratory (ARL), funded a 10-year research program, the Robotics Collaborative Technology Alliance (RCTA), which brought together scientists and engineers from government, academia, and industry to develop autonomous mobile robot technologies focused on solving the unique research challenges of operating in unstructured environments. While the underlying motivation came from helping soldiers operating in Army-relevant environments, the benefits extend to scenarios ranging from disasters, to planetary exploration, to reducing reliance on extensive prior knowledge in structured environments. Initially, the program focused around four key robotics technology areas: perception, intelligence, human-robot interaction, and dexterous manipulation and unique mobility (DMUM). In the program’s last several years, focus shifted to integrated capabilities, built upon the pillars of success already achieved. This revised, system-oriented approach laid the foundation for many experiments in laboratory, simulation, and field environments. The program concluded with a capstone demonstration that showcased advanced robotic capabilities for the stakeholders. This special issue highlights robotics technology developed during the RCTA program, progress of which was evaluated and documented through research articles, field experiments, and hardware/software system descriptions. The program produced hundreds of published papers describing innovations in multiple, autonomy-related research areas throughout the program. The papers selected for this special issue describe more recent work, and range from individual research advances to lessons learned from conducting system-level field experiments.