Fish diversity and assemblage structure in relation to habitat variables were studied in 15 sites in Panna landscape, central India. The sampling was performed between February-April 2009. Fifty species of fishes belonging to 32 genera, 15 families and four orders were recorded from the study area. Cyprinids were the dominant assemblage members in all study streams (abundance ranges from 56.6-94.5 %). The cyprinid Devario aequipinnatus and the snakehead Channa gachua had highest local dominance (80% each) in Panna landscape. High Shannon and Margalef’s diversity was recorded in Madla region of Ken River. Similarity cluster analysis explained the study sites along Ken River (Gahrighat, Magradabri and Madla) had similar faunal assemblage. Canonical Correspondence Analysis (CCA) was performed to study the species association with a set of environmental variables. The CCA revealed that cyprinid abundance was associated with stream order, deeper habitat, flow and water temperature.