The study evaluates irrigation water quality in the Danube-Tisa-Danube hydrosystem area (Vojvodina, northern Serbia). The area is dominantly a plain with about 75% arable land, suitable for agricultural production and irrigation. Use of water of inadequate quality can have long-term effects on irrigated land and affect the yield of cultivated crops. The analyses included data from 40 surface water and 23 groundwater quality monitoring locations, observed during the period 2013-2018. The average annual values of the concentrations of major cations and anions and of electrical conductivity in surface and groundwater were comparatively analyzed. These values were statistically significantly higher (by p < 0.05) in groundwater bodies with most of the analyzed parameters. Hydrochemical classification of water types shows that 95% of surface and 87% of groundwater locations belong to the Ca·Mg-HCO3 water type. Water suitability for irrigation was assessed using specific parameters and indices (sodium adsorption ratio, Na%, residual sodium carbonate, magnesium hazard, permeability index, and Kelly's index). The results showed that surface and groundwater resources are generally of good quality and suitable for irrigation, with sporadic deviations at several locations. The principal component analysis (PCA) was used to identify the most important variables affecting the chemical composition of the analyzed waters and group the monitoring locations by their chemical characteristics. The spatial variation of the analyzed water quality indices was shown on thematic maps.