Kampinos National Park Research Articles

Mapping forest tree species and its biodiversity using EnMAP hyperspectral data along with Sentinel-2 temporal data: An approach of tree species classification and diversity indices

Forests play a crucial role in maintaining ecological balance and biodiversity, making the accurate mapping of tree species and assessment of biodiversity indices essential for informed management decisions. This study introduces an innovative methodology that integrates EnMAP (Environmental Mapping and Analysis Program) hyperspectral data with Sentinel-2 multitemporal data to classify tree species in the biodiverse landscapes of Kampinos National Park and its surrounding regions in Poland.We extract essential vegetation indices such as NDVI, NDMI, SAVI, and EVI from Sentinel-2 data to assess forest health and dynamics. The Sentinel-2 data is upscaled from 10 m to 30 m to align with EnMAP’s spatial resolution, followed by precise co-registration of the images using QGIS. Utilizing a rich dataset from the National Forest Inventory, we extract spectral signatures of nine distinct tree species from both data sources. We employ five machine learning algorithms—Support Vector Machines (SVM), Random Forest (RF), CatBoost (CAT), Gradient Boosting Classifier (GBC), and XGBoost (XGB)—to enhance classification accuracy.Through iterative experimentation with data reduction techniques and algorithm tuning, we achieve optimal performance across needle-leaved and broad-leaved species. The resulting tree species maps are validated through quantitative accuracy assessments against mixed-species polygons from the National Forest Inventory and ground truthing in the Kampinos National Park. Achieving an overall accuracy of 85% to 93%, our study demonstrates the efficacy of this integrated approach in tree species mapping. Furthermore, the tree species maps serve as a foundation for deriving key biodiversity indices—species richness, Shannon-Wiener Diversity Index, Simpson’s Diversity Index, and a composite Biodiversity Index—providing insights into spatial biodiversity patterns and informing targeted conservation strategies. This study exemplifies the potential of combining advanced remote sensing techniques with field validation to enhance our understanding of forest ecosystems and guide sustainable management practices.

Ecological amplitude and indication potential of mining bees (Andrena spp.): a case study from the post-agricultural area of the Kampinos National Park (Poland)

The mining bee (Andrena spp.) play a key role in ensuring plant and animal diversity. The present study examines their diversity in a post-agricultural landscape exemplified by the Kampinos National Park (KNP), a UNESCO Biosphere Reserve in Poland. The following hypotheses were addressed: (H1) the mining bees demonstrate a narrow ecological amplitude, (H2) there are no indicator species for particular habitats, and (H3) the studied mining bees have the same ecological preferences to those presented in the literature. A total of 40 catch per unit effort samples (CPUE) were collected across various habitats with different soil humidity. Forty-six species were recorded, representing 46% of mining bees and approximately 10% of the known Polish bee fauna. Nineteen of the recorded species (41%) were assigned to CR-NT threat categories, indicating that the national park plays a significant role in preserving mining bee species diversity and their conservation. None of the hypotheses (H1, H2, H3) were confirmed. The mining bees were found to demonstrate a wide ecological amplitude. Surprisingly, habitats located in dry and wet soils were both characterised by high abundance and species richness. Seventeen indicators were distinguished among the dominant and rarer species. Our findings suggest that Andrena nigroaenea and A. ventralis (lower humidity), as well as A. alfkenella and A. minutuloides (higher humidity), have different significant relationships with habitat soil humidity to those reported in the literature.

The physical, mechanical, and chemical properties of black cherry tree wood (Prunus serotina Ehrh.) and its susceptibility to fungal decomposition in areas where it is secondary and invasive: a case study in the Kampinos National Park (Poland)

The properties of black cherry mature wood (Prunus serotina Ehrh.) and its susceptibility to fungal decay were studied in the invaded range of the species on six trees aged between 39 and 47 years old growing in poor, acidic soils with varying levels of moisture and organic carbon and nitrogen content. Wood from trees that grew in wetter and richer soil had better physical properties. Of the 95 parameters analyzed, 80 showed significant differences in favor of this wood. These differences included wider rings that averaged 3.25 mm, a higher density of 662.71 kg/m3 at 12% humidity, and 1.5 times higher content of extractives. Gas chromatography with mass spectrometry revealed the presence of 44 extractives. Out of these, six had antifungal properties and were found in the wood of trees grown in richer soil, corresponding to 62.93% of the peaks area of all identified substances. Only three were found in trees grown in poorer soil, corresponding to 8.68% of the peaks area respectively. The wood of trees grown in more fertile soil was also less prone to fungal decay, which was generally low. Only Trametes versicolor caused a mass loss of more than 10% of the wood in both sites out of the four basidiomycete species tested. The results indicate that even slight variations in soil fertility and moisture can benefit black cherry, leading to differences in wood features and properties in its exotic range.

Przyrodnicze obszary prawnie chronione w województwie mazowieckim w latach 1999–2022

The article discusses the changes in the scope of natural legally protected areas, that occured after the administrative reform in 1999. It indicates the tasks carried out by the Self-Government of the Mazowieckie Voivodeship in the field of regional policy and spatial management in accordance with the principle of sustainable development. The Mazowieckie Voivodeship – created under the territorial division reform – covered a system of protected areas that included the Kampinos National Park, 164 nature reserves, 9 landscape parks and 29 protected landscape areas. In nearly 25 years, 26 nature reserves and one protected landscape area were created. The boundaries of the existing forms of nature protection have also been modified. With Poland's accession to the European Union, the most significant changes concerning legally protected areas have taken place. The implementation of EU law into Polish law resulted, inter alia, in the introduction of a new form of nature protection – Natura 2000 sites, complementing the existing system of legally protected areas. By March 2023, 80 areas of the Natura 2000 network had been designated and approved in the Mazowieckie Voivodeship. The article discusses how the position and competences of the regional self-government in the field of nature protection have evolved as a result of changes in the law. The voivodeship self-government took over competences in the field of landscape parks and protected landscape areas. As a result of the acquired rights, the Mazovian Voivodeship Sejmik, inter alia, established the Mazovian Landscape Parks complex and protection plans for landscape parks. The boundaries of the protected landscape areas have been specified. Spatial databases are also maintained in the field of protected areas. Key words: Mazowieckie Voivodeship, nature conservation, legally protected areas, landscape parks, protected landscape areas, green belt

Mites Living in the Nests of the White Stork and Black Stork in Microhabitats of the Forest Environment and Agrocenoses.

The White Stork (Ciconia ciconia) and the Black Stork (Ciconia nigra) are well-known model organisms for the study of bird migration, as well as the selectivity of nesting sites and the choice of living environment. The former breeds mainly in open areas, while the latter inhabits forest areas. The acarofauna, and in particular Oribatida, inhabiting the nests of these species, has not been thoroughly explored so far. Therefore, we analyzed the material collected from 70 White Stork nests and 34 Black Stork nests in Poland, between Poznań and Rawicz, and in Kampinos National Park. Our research has increased the faunal and ecological knowledge of the mite fauna inhabiting the nests of large migratory bird species. Oribatida constituted 5-12% of the total mites identified in the nests of White and Black Storks. Their average number was several times higher in the Black Stork nests (80.2 individuals in 500 cm3). Also, the species diversity of moss mites was greater in the Black Stork nests (47 species). In total, the nests of the two stork species were inhabited by 62 moss mite species, with only 22 recorded in both the White and the Black Storks' nests. The most numerous species included Ramusella clavipectinata, R. fasciata, Oppiella subpectinata, Acrogalumna longipluma, and Scheloribates laevigatus. In addition, we found that juvenile oribatid mites accounted for 0.6% of all the mites in the White Stork nests, with tritonymphs having the largest share, while juveniles in the Black Stork nests comprised 1.4%, of which larvae and protonymphs had the largest share. Our research shows that the nests of large migratory birds provide living space for many mite species. In addition, we noted the potential importance of White and Black Stork nests for mite dispersion and the evolution of interspecies interactions.

Contribution to the Knowledge of Fungi of the Kampinos National Park (Central Poland): Part 6 – With Particular Emphasis on the Species Occurring on Windthrown Areas

Abstract We identified 17 species of fungi that are new to Kampinos National Park. Sixteen were found during surveys of areas damaged by a strong wind in 2017. The remaining species was found outside the windthrow area. Descriptions of four species new to Poland ( Cortinarius subcompar , Hyaloscypha quercicola , Hypocrea tremelloides , and Trechispora aff. invisitata ) are also provided. The current number of macromycetes taxa identified in Kampinos National Park is 1,654.

Quantitative and qualitative assessment of pine seedlings under controlled undergrowth disturbance: Fire and soil scarification

Scots pine (Pinus sylvestris L.) is the most widespread forest tree species in Central Europe. The range of Scots pine depends on the influence of forest management on stand species composition, as the potential for the natural regeneration of Scots pine monocultures is smaller than the current range of the species. To achieve regeneration, pine requires specific ecological conditions, including adequate soil preparation. The literature notes that the effective natural regeneration of pine requires fire or mixing the organic soil layer with the mineral layer. This hypothesis was critically evaluated carrying out work with the main objective of comparing the germination and growth dynamic of pine seedlings in two variants, simulating fire or soil scarification against natural conditions. The research focused on analyzing the growth of pine seedlings from germination to the final number of seedlings, which remained unchanged until the end of the experiment. The evaluation was carried out in soil monoliths from Kampinos National Park (KNP), in which seeds from a homogeneous mother stand were planted. The quantitative and qualitative characteristics of the seedlings were statistically evaluated, including analyses of their root system characteristics. The results confirmed the positive effect of mixing an organic and mineral layer at the germination stage and during the subsequent growth of the pine. The seedlings had a high survival rate (65.3%). However, the positive effect of fire on the regenerative capacity of pine could not be confirmed; the number of obtained seedlings (29.5%) was significantly lower than in the control variant. In addition, the “fire variant” was characterized by high seedling mortality immediately after germination. Root systems were important for the survival of the seedlings, the development of which was affected by the tested variant. The analyses performed may have implications for the development of research on the possible natural regeneration of Pines after natural disturbances. Additional topic that needs further research is the response of seedling root systems to changes in soil conditions.

Critical check-list of spiders (Araneae) of the Kampinos National Park

This paper presents the results of the research conducted so far on Araneae of the Kampinos National Park. After verification of the published data, it was established that 305 species of spiders recorded in the Park area; this is 36.8% of Araneae reported from Poland. The results of the study so far indicate that Kampinos NP is characterized by a very great diversity of araneofauna. In the Park have been found four protected species: Agroeca dentigera, Eresus kollari, Philaeus chrysops, Yllenus arenarius and many rare and interesting spider species, e.g. Archaeodictyna ammophila, Enoplognatha mordax, Haplodrassus moderatus, Kishidaia conspicua, Titanoeca spominima and many others. Three species: Micaria micans, Pardosa alacris and Sibianor larae are reported for first time from this park.

Vascular flora changes in canopy gaps caused by wind

Abstract A study on the importance of wind-induced canopy gaps was conducted in Kampinos National Park (Poland) between2018 and 2021. Two types of habitats were considered – dry mesotrophic oak forest and wet Scots pine forest (Molinio- Pinetum). Canopy gaps were characterized by higher plant species richness than in adjacent areas, and their effect was weaker in oligotrophic coniferous forest than in mesotrophic deciduous oak stand.

Local Spatial Plans in Supporting Sustainable Water Resources Management: Case Study from Warsaw Agglomeration—Kampinos National Park Vicinity

The crisis resulting from progressive climate change is reflected in increasingly violent problems with periodic excesses and shortages of water. Integrated water management has thus become a necessity and it depends, inter alia, on the effectiveness of the adopted strategies, policies and individual investments. In many countries, including Poland, the implementation of this postulate is slow. The importance of local spatial plans, which are a tool that directly translates adopted policies into spatial development, is underestimated. The article presents studies of the provisions of planning documents with particular emphasis on the local spatial plans regulations in terms of considering the issue of water management. Some municipalities in the Warsaw agglomeration were selected for the detailed research, as the areas characterized by various water conditions. Local spatial plans adopted for this territory were compared to model planning acts from other regions of Europe. The conclusions include recommendations for local spatial plans and a method for assessing planning documents regarding the scope of the sustainable water management arrangements. The study results indicate that the principles of integrated water management have not been fully incorporated into the existing plans in Poland and that national and regional policies are poorly translated into real management planning.

Contribution to the Knowledge of Fungi of the Kampinos National Park (Central Poland): Part 5 – With Particular Emphasis on the Species Occurring on Windthrown Trees

Abstract The paper presents eleven species of fungi new to the Kampinos National Park. They were found during the studies on wind-damaged areas after the forest was damaged in 2017. Two species new to Poland were described and illustrated ( Nectria nigrescens and Strossmayeria basitricha ). The current number of macromycetes taxa known from the Kampinos National Park has reached 1,637.

Conservation of Genetic Diversity of Scots Pine (Pinus sylvestris L.) in a Central European National Park Based on cpDNA Studies

In the old pine stands of national parks, it is possible to observe genetic processes in a state free from disturbance by humans. Studies of this type make it possible to evaluate the effectiveness of the conservation of genetic variation and its transfer between generations. The present study was conducted in the largest national forest park in Poland, located in the Central European pine area. The oldest stands of Kampinos National Park and their natural descendants were selected for detailed analyses. The main objective of the study was to compare the mother pine stand, excluded from forest management, with its progeny generations on the basis of their chloroplast DNA (cpDNA), which was used as a diagnostic tool. The results demonstrate significant genetic difference between the maternal and progeny generations of the studied sites. The degree of variation observed in the maternal generation haplotypes in the present study was found to be reduced in the next generation. A significant proportion of the genetic diversity of the studied stands was also lost in the subsequent progeny generation. The obtained results allow conclusions to be drawn about the genetic processes taking place in valuable old-growth forests.

Biomonitoring of Heavy Metal Air Pollution in Warsaw Using Two Moss Species Pleurozium schreberi and Sphagnum palustre

The aim of the study was to assess air pollution with heavy metals in Warsaw, on the basis of the concentrations of selected elements in moss samples. The active biomonitoring method (moss-bag technique) was applied using two moss species Pleurozium schreberi and Sphagnum palustre. Moss samples were collected in the Kampinos National Park, and the prepared moss bags were distributed and exposed on seven sites in Warsaw. The analysis of metals accumulated in mosses was performed twice in 2020, after two (August-September) and four months (August-November) of exposure. The concentrations of seven heavy metals (Cr, Cu, Pb, Ni, Fe, Cd and Zn) in the mosses were determined, using an Inductively Coupled Plasma Optical Emission Spectrometer (ICP OES). Our results showed a clear dependence of heavy metal accumulation in the mosses on the location of the exposition site and the exposure period. Both species of mosses were found to accumulate the most metals in the vicinity of pollutant emitters, such as the ArcelorMittal Warsaw smelter, exit roads or roads in the city with heavy traffic, petrol stations, or construction works. After 4 months of exposure, in both moss species, the highest increases in the concentrations were found for four elements: Cr, Pb, Ni and Cd. Higher concentrations of some heavy metals in the mosses in 2020, as compared to previous studies, indicate a negative influence of progressing urbanisation on air pollution in Warsaw.

Contribution to the Knowledge of Fungi of the Kampinos National Park (Central Poland): Part 4 – With Particular Emphasis on the Species Occurring on Windthrown Trees

AbstractThis paper presents 18 species of fungi that are new to the Kampinos National Park and their buffer zone. Seventeen of these species were found during studies on wind-damaged areas after the forest was damaged in 2017. One species new to Poland has been described and illustrated (Odonticium septocystidiatum). A few of the species recorded (Ciliolarinacfr.laricina,Daldinia petriniae,Pseudovalsa umbonata,Spongipellis litschaueri) are very rare in Poland and hitherto mentioned from single localities in the country. Two species from the Polish red list of macrofungi were recorded in the Kampinos National Park for the first time – namely,Punctularia strigosozonata(E) andTrichaptum biforme(R). The current number of macromycete taxa known from Kampinos National Park has reached a total of 1,630.

Zmiany składu gatunkowego i wskaźników struktury zbiorowisk roślinnych w latach 2001‑2018 na wybranych powierzchniach stałych w Kampinoskim Parku Narodowym = Changes of species composition and structural indices among plant communities in 2001‑2018 on selected permanent plots in Kampinos National Park

On the basis of 2001, 2007, 2012 and 2018 phytosociological records obtained from 52 permanent plots distributed across Poland’s Kampinos National Park, it proved possible to determine main directions of change in the ecological character of undergrowth. Reference to similarity of species composition on the plots between 2001 and 2018 allowed for the identification of Groups: A (occurring in boggy alder forest habitats, and comprising communities at different successional stages), B (coniferous and mixed-coniferous forests), C (oak-hornbeam forests and similar communities) and D (vegetation representing different dynamic stages of the succession from non-forest to forest communities). Irrespective of each plot’s unique history, the above Groups of plots could be characterised in line with specific sets of features subject to directional change during the period under analysis. Where Group A communities were concerned, a rise in the level of ground water and a decline in the content of organic carbon in soil were experienced, with the result that fen sedge species (Scheuchzerio-Caricetea) came to play a reduced role, even as there was a steadily-increasing role for species associated with communities flooded at least periodically (hence Potamogetonetea and Bidentetea). This was accompanied by a decrease in the number of shrub species and an increase in the number of bryophyte species. There were also increases in the proportions of geophytes and species associated with habitats more abundant in nitrogen. Group B communities were likewise subject to rising water tables, albeit changes not reflected directly in differing vegetation characteristics. The carbon: nitrogen ratio in soils here increased, as did numbers of species characteristic of undergrowth, as well as the bryophyte layer. Nevertheless, where undergrowth species were concerned, the role of those typical for open sandy grasslands (Koelerio-Corynephoretea) is seen to be declining, with this inter alia denoting increased shares of species either tolerant of shade or avoiding full illumination. There has also been a decline affecting hemicryptophytes, even as species associated with more nitrogen-rich habitats have come to account for greater shares. Where Group C communities encountered higher water levels, certain patches present in wetter habitats saw increases in the role played by species characteristic for class Bidentetea. The sorption capacities of soils here decreased, while values for the C: N ratio rose. Tree and shrub species came to be fewer in number as numbers of undergrowth species rose. There were also increases in the roles of species characterising moist verges (Galio-Urticenea), as well as geophytes. Group D communities likewise encountered rising groundwater levels, but did not seem to experience directional change otherwise, within their phytocoenoses. The sorption capacity of their soils nevertheless decreased. Overall, it was possible to note increases over the period in question when it came to numbers of species present in the tree, undergrowth and bryophyte layers. However, while the role of species characteristic for class Nardo-Callunetea increased, that of Trifolio-Geranietea species declined. And, while correlations between vegetation and soil characteristics proved to be different for each of the four groups of communities, there was no precluding model parameters differing significantly from one year of measurement to another.

Revisiting the reintroduced Eurasian lynx population in Kampinos National Park, Poland

In the past centuries, many of the Eurasian lynx populations declined and finally disappeared. In Poland, only two populations survived until the 20th century. In the 90s a new population was reintroduced in central Poland but presently, its fate is considered uncertain. While the recurring observations of lynx in the area confirm its presence, they are insufficient to evaluate the state of this population. Therefore, using a population viability model and the reintroduction program data, we analyzed the ambiguities in the vital rates assessments to find the most likely scenario for the development of the population since its reintroduction. Finally, we modeled different options to improve the survival chances of this one and possibly other small populations. Estimated parameters result in a declining population. In the majority of the simulations, lynx populations go extinct within 20 years. This can be improved by reintroduction or natural immigration of new individuals every 4 years, but only if the new individuals arrive within a short period of time after the initial reintroduction. Also, longer time intervals are insufficient to elevate the median time to extinction beyond 50 years. Separately, moderate changes in reproduction or survival rates have only a marginal effect on the population’s survival. Decreasing only the impact of mortality would increase this population’s persistence time, but not ensure its long-term survival. However, decreased mortality in combination with immigration should result in much more successful population development. With higher longevity and release every 4 years the population is more successful even if the new individuals start entering the population up to 20 years later. Even less frequent immigration combined with enhanced longevity still improves survival. Combined adjustment of individuals to their environment and regular immigration provide the best chances of long-term survival. One or both of those factors could explain why lynx is still present in the area.

Monument trees in the Kampinos national Park (central Poland): A review

Abstract The paper presents the history of the protection of trees with special values, the current state of monument trees and the preliminary description of trees meeting the minimal circumference criteria to be regarded as natural monuments in the Kampinos National Park (KNP). The study was conducted in the years 2017–2019. In the KNP, there are 69 living trees with a status of natural monuments. Most of them are oaks – 56 specimens, Scots pine – 6 specimens, small-leaved lime – 5 specimens and European hornbeam and European ash – 1 specimen each. Among all, 27 trees grow individually and the other 42 grow in 7 groups. About 200 trees were recognised as meeting the minimal circumference criteria to be regarded as natural monuments. The thickest size tree in the KNP is the black poplar with a circumference of 805 cm, growing in the enclave of Ruska Kępa, and the thickest monument tree in the KNP area is Dąb Kobędzy (Kobendza Oak) with a circumference of 582 cm. Almost a half of the living monument trees (34 specimens) are situated in Kampinos Forest District. In Kromnów, there are 20, and in Laski Forest District 15 specimens of monument trees are mapped. Living monument trees were found in 15 (out of 17) forest subdistricts. The highest number of monument trees was found in Rózin Forest Subdistrict (16) and in Wilków Forest Subdistrict (10). The lowest number – one specimen per forest subdistrict – was found in six forest subdistricts (Dąbrówka, Grabina, Janówek, Krzywa Góra, Rybitew and Zamczysko). In total, there are around 300 specimens of natural monuments and trees meeting the minimal circumference criteria to be regarded as natural monuments, which means that there is one tree of this category for each 125 ha surface of the KNP.

Temporal and Spatial Diversity of Renewable Groundwater Resources in the River Valley

Quantitative assessment of the amount of renewable resources allows their evaluation, valorization and protection in terms of the possibility of their environmental management under climate change conditions. The aim of the study was to determine the amount of renewable resources in the Middle Vistula valley, in the region of the Kampinos National Park, central Poland. The amount of renewable resources was calculated on a hydrodynamical model for three variants, as the average, the lowest and the highest infiltration recharge rate for a specified period of 1999–2013. The modelling research was conducted in a strongly differentiated hydrogeological valley unit, in which several geomorphological units could be delineated: the floodplain, over-flood terraces and the plain area. The hydrodynamic modelling results were verified by comparing the obtained data with both the amount of drainage in the valley zone and the underground streamflow. The assessment of renewable groundwater resources in three distinctive variants was the basis for calculating the groundwater footprints, defined as a quantitative assessment of the groundwater use in climate change conditions.

First record of Agrilus hastulifer (Ratzeburg, 1837) (Coleoptera: Buprestidae) in Poland with data of its bionomy, distribution, morphology and identification

The paper presents the first records in Poland of Agrilus hastulifer (Ratzeburg, 1837) (Buprestidae). It is the 31st species of the genus Agrilus Curtis, 1825 recorded in the country. It was found in two locations in eastern (Białowieża Primeval Forest) and central Poland (Kampinos National Park). All data of the biology and economic significance of this species, based on the literature and our observations, are summarized. Due to the great difficulty in distinguishing this species from Agrilus graminis Kiesenwetter, 1857, we also provide an original, richly illustrated comparison useful for discriminating the two species.

Black cherry (Prunus serotina Ehrh.) colonization by macrofungi in the fourth season of its decline due to different control measures in the Kampinos national Park

Abstract The experiment conducted in the Kampinos National Park since 2015 was aimed at assessing the sprouting ability of black cherry (Prunus serotina Ehrh.) in response to different measures of mechanical control and mycobiota colonizing the dying trees. Basal cut-stump, cutting at ca. 1 m above the ground and girdling were performed on 4 terms, two plots and applied to 25 trees, 600 trees in total. Sprouts were removed every 8 weeks since the initial treatment for 4 consecutive growing seasons, except winter-treated trees. At the end of the fourth season of control, 515 out of 600 trees were dead (86%): 81% on Lipków and 90% on Sieraków plot. Among 18 experiment variants with sprouts removal, 17 showed more than 80% of dead trees. The lowest, 76% share, concerned summer cut-stump at the base of the tree. For winter measures, the share of dead trees was lower in all cases and ranged from 28% to 64% proving that sprouts removal contributes to the drop of sprouting strength and quicker dying of the trees. Almost 80% of trees showed sporocarps that represented 51 taxa of macrofungi in total, including 6 Ascomycota and 45 Basidiomycota. The group of six most frequently encountered fungi includes: Hyphoderma setigerum, Bjerkandera adusta, Peniophora cinerea, Armillaria ostoyae, Nectria cinnabarina, Stereum hirsutum. Both plots had similar share of black cherry individuals with sporocarps of macrofungi, that is, 81% and 78% for Sieraków and Lipków respectively. The share of colonized trees and the number of reported macrofungal taxa increased significantly compared to the year following the treatment. In addition, the composition of macrofungi changed with the progressing dying of trees. These results broaden the knowledge about macroscopic fungi colonising and living on black cherry within its secondary range of distribution. Moreover, one macrofungus and two microfungi new for KNP are reported.