Juvenile Red Research Articles

Beneficial Effects of Dietary Bacillus subtilis and Citric Acid Supplementation on Growth Performance, Feed Efficiency and Body Composition of Juvenile Red Hybrid Tilapia (O. Niloticus×O. mosambicus)

Beneficial Effects of Dietary Bacillus subtilis and Citric Acid Supplementation on Growth Performance, Feed Efficiency and Body Composition of Juvenile Red Hybrid Tilapia (O. Niloticus×O. mosambicus)

Uses of Coconut Residue from Coconut Oil with Extracted <i>Curcuma longa</i> Production for Juvenile Red Claw Crayfish Feeding

Coconut protein residue from production of coconut oil with extracted Curcuma longa has high antioxidant as curcuminoids, and high protein which are needed for growth performance and survival rate of Juvenile red claw crayfish (Cherax quadricarinatus). The objective of this work was to study the effects of coconut protein residue from production of coconut oil with extracted Curcuma longa in feed to growth performance and survival rate of Juvenile red claw crayfish. The experiment was carried out using plastic bucket systems with three treatment conditions: T1 used pellet (control); T2 used cream protein from virgin coconut oil (VCO); T3 used cream protein from coconut oil with extracted Curcuma longa (COC), respectively. Feed with cream protein from COC and VCO has significant effect on weight gain and feed conversion ratio (FCR) of Juvenile red claw crayfish when compared to Juvenile red claw crayfish in the control diet. FCR in the control group was significantly (p<0.05) higher than those of the treated groups. Weight gain was highest in the T3. Survival rate of every treatment was 100%. The result indicated that the cream protein from COC with high protein and high antioxidant is a potential additive in the Juvenile red claw crayfish diet.

Replacement of Menhaden Fish Meal by Poultry By‐Product Meal in the Diet of Juvenile Red Porgy

AbstractPoultry by‐product meal (PBM) was tested as a substitute for fish meal (FM) in the diet of juvenile Red Porgy Pagrus pagrus. Eight diets (50% crude protein, 13% crude lipid) were formulated to replace FM protein with PBM protein at 0 (control), 10, 20, 30, 40, 50, 60, and 70%. Juvenile Red Porgy were fed the diets twice daily to apparent satiation for 8 weeks. Fish were held in 75‐L rectangular tanks with 15 fish/tank using a recirculating seawater (34 g/L) system maintained at 22°C. No significant differences in body weight gain (BWG; 318–387%), final weight (17.4–20.4 g), specific growth rate (2.55–2.77% per day), feed conversion ratio (0.85–1.18), protein efficiency ratio (0.45–0.61), or survival (84–98%) were observed among fish fed diets replacing 0–70% of FM protein with PBM protein. After the feeding trial, the survival, growth, feed utilization, fish whole‐body protein, lipid, docosahexaenoic acid content, and apparent digestibility of protein were not significantly different for fish fed diets with up to 70% of FM protein replaced by PBM compared to a 100% FM‐protein‐based control diet. Broken‐line regression analysis with BWG indicated that PBM protein can replace FM protein in Red Porgy diets at levels as high as 54.0% with no reduction in fish growth performance. Poultry by‐product meal is a cheaper and more sustainable ingredient than FM and is an excellent alternative protein source for Red Porgy.

Identification, Characterization, and Mapping of a Novel SNP Associated with Body Color Transparency in Juvenile Red Sea Bream (Pagrus major).

We previously reported a body color deformity in juvenile red sea bream, which shows transparency in the juvenile stage because of delayed chromatophore development compared with normal individuals, and this finding suggested a genetic cause based on parentage assessments. To conduct marker-assisted selection to eliminate broodstock inheriting the causative gene, developing DNA markers associated with the phenotype was needed. We first conducted SNP mining based on AFLP analysis using bulked-DNA from normal and transparent individuals. One SNP was identified from a transparent-specific AFLP fragment, which significantly associated with transparent individuals. Two alleles (A/G) were observed in this locus, and the genotype G/G was dominantly observed in the transparent groups (97.1%) collected from several production lots produced from different broodstock populations. A few normal individuals inherited the G/G genotype (5.0%), but the A/A and A/G genotypes were dominantly observed in the normal groups. The homologs region of the SNP was searched using a medaka genome database, and intron 12 of the Nell2a gene (located on chromosome 6 of the medaka genome) was highly matched. We also mapped the red sea bream Nell2a gene on the previously developed linkage maps, and this gene was mapped on a male linkage group, LG4-M. The newly found SNP was useful in eliminating broodstock possessing the causative gene of the body color transparency observed in juvenile stage of red sea bream.

Demonstration that Feeds Containing <1% Fishmeal Can Support Grow‐out of Large Juvenile Red Drum, Sciaenops ocellatus, and Reduce Nutrient Waste

AbstractWe conducted a 16‐wk feeding trial with large juvenile red drum, Sciaenops ocellatus. Four diets were randomly assigned to six replicate tanks per treatment. Three isonitrogenous (ca. 44.5% protein) and isolipidic (ca. 14.1% lipid) extruded diets were formulated to compare a fishmeal‐based diet with diets using alternative protein sources. Diet 1 contained 19.60% fishmeal and 21.42% poultry meals as primary protein sources. Two alternative diets were formulated reducing the fishmeal to 0.61% by substituting poultry meals (33.85%) and soybean protein concentrate (11.55% in Diet 2 and 11.70% in Diet 3). Diet 3 also included Allzyme Vegpro® and Allzyme® SSF at 0.04%. Diet 4, a natural diet consisting of chopped cigar minnows, squid, and shrimp, was used as a positive control to compare growth rates of formulated feeds to near maximum growth under these culture conditions. We found that reducing the amount of fishmeal to <1% by using alternative protein sources did not affect the growth rate, survival, or health of red drum but improved assimilation of phosphorus, reduced potential release of P to the environment, and significantly lowered the amount of feeder fish needed in feed. The control diet identified performance benchmarks for future feeds development work.

Tracking Larval, Newly Settled, and Juvenile Red Abalone (Haliotis rufescens) Recruitment in Northern California

ABSTRACT Recruitment is a central question in both ecology and fisheries biology. Little is known however about early life history stages, such as the larval and newly settled stages of marine invertebrates. No one has captured wild larval or newly settled red abalone (Haliotis rufescens) in California even though this species supports a recreational fishery. A sampling program has been developed to capture larval (290 µm), newly settled (290–2,000 µm), and juvenile (2–20 mm) red abalone in northern California from 2007 to 2015. Plankton nets were used to capture larval abalone using depth integrated tows in nearshore rocky habitats. Newly settled abalone were collected on cobbles covered in crustose coralline algae. Larval and newly settled abalone were identified to species using shell morphology confirmed with genetic techniques using polymerase chain reaction restriction fragment length polymorphism with two restriction enzymes. Artificial reefs were constructed of cinder blocks and sampled each year ...

Survival and Physiological Responses of Juvenile Red Drum Exposed to Hypersalinity and Elevated Temperatures

AbstractFingerling Red Drum Sciaenops ocellatus were held in water recirculating systems for 4–5 weeks under selected combinations of temperature and salinity: 30°C and 35‰ (reference environment), 34°C and 45‰, or 34°C and 55‰. At the end of the culture period, condition factor, hematocrit, plasma osmolality, plasma lactate concentration, plasma glucose concentration, and liver glycogen content were determined. Responses of fish in the warm, hypersaline treatments did not differ significantly from fish in the reference treatment. Based on the results of this study, juvenile Red Drum can tolerate warm, hypersaline conditions for several weeks with no apparent detrimental effects if slowly acclimated to environmental temperature and salinity as long as food is not limiting and other water quality characteristics are acceptable.Received October 2, 2015; accepted January 4, 2016

Influence of saHnity and food deprivation on growth and RNA-DNA ratio in red drum Sciaenops ocellatus (Pisces: Sciaenidae)

Juvenile Red drum Sciaenops ocellatus held in 30 ppl natural seawater or fresh water were starved or fed ad libitum for su weeks, and sampled each week for growth and for determination of RNA, DNA, and protein contents of white and red muscles and liver. Specific growth Tate and condition factor were used lo compare changes in nucleic acid and protein concentrations in fish tissues. Salinity had liule effect on growth, while starvalIon caused a significant reducuon in all growth parameters and in the RNA-DNA ratio after three weeks. The RNA-DNA ralIos of red and white muscles of the fed fish were greater than those of the starved fish and also were related to specific growth Tate and fish condition factor. Nucleic acid concentralIons in liver were highly variable. Since wrute muscle predorninates and is more easily sampled in red drum than red muscle, and because it has less variable RNA-DNA ratios than liver, it is the tissue recornmended for nucleic acid analysis of physiological condition and growth.

Growth Responses of Juvenile Red Drum Sciaenops ocellatus to Dietary Phenylalanine and Tyrosine Can Be Used to Calculate the Total Aromatic Amino Acid Requirement ,

A current priority in aquaculture is the replacement of fish meal with alternative feedstuffs to ensure the industry's sustainability. However, most alternative protein sources are deficient in at least 1 indispensable amino acid (IAA). Therefore, there is a critical need to establish refined estimates of IAA requirements of fish. The objectives of this study were to determine the total aromatic amino acid (TAAA) requirement (Phe + Tyr) and the Tyr replacement value for Phe in juvenile red drum Sciaenops ocellatus. The TAAA requirement was obtained by feeding juvenile red drum diets that contained incremental amounts of Phe (0.54, 0.84, 1.14, 1.44, 1.74, and 2.04 g/100 g dry diet) and a fixed concentration of Tyr. Because of the TAAA requirement obtained, a second feeding trial was conducted to determine the maximum Tyr replacement value for Phe when a control diet was prepared with an 80%:20% Phe-to-Tyr ratio (Phe:Tyr; 1.60 g Phe/100 g dry diet and 0.41 g Tyr/100 g), and 5 experimental diets were prepared by decreasing the inclusion of Phe and increasing the inclusion of Tyr (Phe:Tyr of 70%:30%, 60%:40%, 50%:50%, 40%:60%, and 30%:70%). Weight gain, the feed efficiency ratio, and the protein efficiency ratio increased 354%, 133%, and 134%, respectively, relative to that of fish fed the basal diet as the Phe concentration increased to 1.44 g/100 g of dry diet; at higher concentrations of Phe, no significant differences were found between treatments. Analysis of the weight gain data with a broken-line model estimated the TAAA requirement of red drum to be 2.10 g/100 g dry diet (1.69 g Phe + 0.41 g Tyr). The maximum Tyr replacement value for Phe was estimated at 40%:60% Phe:Tyr, because only fish fed the diet with a 30%:70% Phe:Tyr ratio had a significant reduction (42%) in growth performance. The TAAA requirement for maximum growth of juvenile red drum was estimated to be 2.10 ± 0.08 g/100 g dry diet. Moreover, Tyr can account for up to 60% of the TAAA requirement of juvenile red drum.

Effects of Taurine, Phytase and Enzyme Complex Supplementation to Low Fish Meal Diets on Growth of Juvenile Red Sea Bream Pagrus major

Effects of Taurine, Phytase and Enzyme Complex Supplementation to Low Fish Meal Diets on Growth of Juvenile Red Sea Bream Pagrus major

Habitat Use by Juvenile Red Snapper in the Northern Gulf of Mexico: Ontogeny, Seasonality, and the Effects of Hypoxia

AbstractRed Snapper Lutjanus campechanus in the northern Gulf of Mexico are threatened by the combined influence of fishing pressures, the bycatch of juveniles in the shrimp trawl fishery, and the seasonal formation of hypoxic (≤2 mg/L O2) bottom water along the Louisiana–Texas continental shelf. Juveniles are especially at risk from hypoxia because they leave the plankton and transition from pelagic to demersal habitats en masse during summer, when hypoxic conditions peak. We explored the influence of hypoxia on juvenile Red Snapper by examining habitat suitability during years with low (areal extent, <10,000 km2), moderate (10,000–19,999 km2), and severe (≥20,000 km2) hypoxia through retrospective analyses of groundfish data from the Southeast Area Monitoring and Assessment Program. Significant interannual variability in the strength of juvenile recruitment was evident, and in general the weakest recruitment was observed during years of severe hypoxia. The relative abundance of juveniles in shallow (9–46 m) waters off central Louisiana was significantly reduced during years of severe hypoxia; this reduction was generally accompanied by an increase in relative abundance of Red Snapper longitudinally and in deeper waters. Patterns of habitat use during summer months varied in relation to hypoxia severity, with individuals occupying sites that were deeper, colder, and of higher salinity during years of severe hypoxia. Red Snapper also exhibited ontogenetic habitat shifts into deeper, cooler, and higher‐salinity waters as fish size increased. In general, Red Snapper populations appear resilient to the broad‐scale effects of severe hypoxia, although more localized effects may be in force but not evident because of the many factors affecting survival.Received June 19, 2014; accepted October 6, 2014

Red coloration in juvenile spiny-footed lizards, Acanthodactylus erythrurus, reduces adult aggression

In many animal taxa, coloration is a visual signal used for communication among conspecifics, for example between age classes. Juvenile coloration has been hypothesized to reduce aggression from adults in some species, in what is called the aggression avoidance hypothesis. Spiny-footed lizards are good subjects for testing this hypothesis, as juveniles develop conspicuous red coloration on their hind limbs and tails that fades in adulthood. To test the influence of juvenile coloration on adult aggressiveness, we conducted videotaped encounters in captivity between adults of both sexes and juveniles with their natural red coloration, or experimentally painted either red or white on their natural red parts. Then we recorded the number of times juveniles were bitten and attacked. In unpainted juveniles, no significant relationship was found between juvenile coloration (brightness, red chroma or hue) and adult aggressiveness. However, juveniles painted red were bitten less than those painted white when number of times bitten was controlled for number of times attacked. This result supports the aggression avoidance hypothesis, as an escalation from low-intensity (attacking) to high-intensity aggression (biting) was less probable towards red juveniles. The presence of red coloration in juveniles caused the reduction in adult aggression, while small natural variations in this red colour did not seem to have any further effect. Juvenile red coloration in this species might indicate age or sexual immaturity to adults. © 2015 The Association for the Study of Animal Behaviour. Published by Elsevier Ltd. All rights reserved.

Rice Distillers Dried Grain Is a Promising Ingredient as a Partial Replacement of Plant Origin Sources in the Diet for Juvenile Red Seabream (<i>Pagrus major</i>)

This study was designed to test the effects of dietary distillers dried grain (DDG) level on the growth performance, feed utilization, body composition and antioxidant activity of juvenile red seabream (Pagrus major). Six isonitrogenous and isocaloric diets were formulated to contain 0%, 5%, 10%, 15%, 20%, and 25% DDG from rice (designated as DDG0, DDG5, DDG10, DDG15, DDG20, and DDG25), respectively. Juvenile red seabream averaging 10.1±0.05 g were randomly distributed into 400-L tanks in a flow through systems. Three replicate groups of fish were fed one of the experimental diets to visual satiation two times a day for 10 weeks. Survival, weight gain, feed efficiency, protein efficiency ratio and hepatosomatic index of fish were not affected by dietary DDG levels (p>0.05). Proximate and amino acid composition of whole body in juvenile red seabream were not affected by dietary DDG levels (p>0.05). Plasma content of total protein, glucose, cholesterol, glutamic-pyruvic transaminase, phospholipid and triglyceride were not affected by dietary DDG levels (p>0.05). 1, 1-Diphenyl-2-picryl-hydrazyl radical and alkyl radical scavenging activities in plasma and liver of fish were not affected by dietary DDG levels (p>0.05). The results of this experiment suggest that DDG has the potential to replace plant origin ingredients such as wheat flour and corn gluten meal and could be used up to 25% in diet without incurring negative effects on the growth performance of juvenile red seabream.

Induction of Primary Male in Juvenile Red Spotted Grouper Epinephelus akaara by Immersion of 17α-Methyltestosterone

We investigated the androgenic effects of 17α- methyltestosterone (MT) on gonadal sex reversal in juvenile red spotted grouper Epinephelus akaara. The fish were immersed in 17α-MT at 1 and 5 mg/L. Treatment method of 17α-MT was once weekly for 4 and 8 weeks. Fish were sampled at 12 months after end of the treatment period in order to histological analysis. At the initiation of an experiment (70 day after hatching), juvenile red spotted grouper have the paired primordial gonads with somatic cells bellow kidney in the posterior portion of the body cavity. Formation of ovarian cavity indicates that the ovarian differentiation beginning at 70 DAH in red spotted grouper. At 12 months after end of the treatment period, control group, 17α-MT 1 mg/L treatment group for 4 and 8 weeks, and 17α-MT 5 mg/L treatment group for 4 weeks were all female. However, sex-changed males without ovarian cavity were observed in the 17α-MT 5 mg/L treatment group for 8 weeks. In grouper, we firstly reported that the red spotted grouper be able to induce the primary males by hormone treatment prior to gonadal sex differentiation.

Hydrolyzed Tuna Meat By-Product Supplement for Juvenile Red Sea Bream, Pagrus major, and its Effect on Growth, Enzyme Activity, Plasma Parameters, and Apparent Nutrient Digestibility

A growth experiment was conducted on juvenile red sea bream, Pagrus major to investigate the effect of the inclusion in fish diets, of tuna meat by-product hydrolysate which was processed through enzymatic hydrolysis using a commercially available enzyme, derived from Bacillus subtilis. Six experimental diets were formulated in the experiment. Three diets contained 50, 150 and 250 g/kg of TPM-H (tuna meat by-product hydrolysate), and two diets with the unprocessed TPM (tuna meat by-product) at an inclusion level of 50 and 250 g/kg. A control diet was formulated without any addition of the test ingredients. Treatment diets were fed ad libitum to juvenile fish with an initial average body weight of 0.81 ±0.13 g for 56 days. Results of the feeding trial suggest that the inclusion of TPM-H at 250 g/kg in fish diets improved body weight gain rate (3271.58%), feed intake (24.55 g/fish/56 days) and feed conversion efficiency (1.12) of the fish. Apparent nutrient digestibility of hydrolyzed tuna meat by-product improved compared to the unhydrolyzed ingredient. These results suggest that TPM processed as hydrolysates can be efficiently utilized by fish.

Influences of Artificial Reefs on Juvenile Red Snapper along the Mississippi Gulf Coast

AbstractRed Snapper Lutjanus campechanus represent one of the more economically important fisheries in the northern Gulf of Mexico; as such, Red Snapper abundance has decreased dramatically in the past two decades. The use of artificial reefs could aid in the rehabilitation of Red Snapper stocks by providing refuge for juveniles and a place of foraging and recruitment. A study was initiated to determine the effectiveness of different artificial reef distribution patterns in attracting and sustaining juvenile Red Snapper in the northern Gulf of Mexico. Fish traps (0.97 m long; 0.64 m high; funnel mouth size = 175×115 mm) were used to collect Red Snapper (<406 mm TL) that were associated with pyramid‐shaped artificial reef structures (3.7‐m triangular base; 2.4‐m height; 3.2 metric tons) to evaluate two reef distribution designs: (1) five closely spaced pyramid units (“clumped” pattern) and (2) five closely spaced pyramids plus two sets of two pyramids at 30.5, 61.0, or 91.5 m from the five pyramids (“outlier” pattern). In 26 sampling trips, 927 Red Snapper were captured. Catch per unit effort (number of fish/trap soak‐hour) did not differ significantly among artificial reef patterns (P = 0.396). Red Snapper TLs differed significantly among patterns (P = 0.005), with the largest mean TL (235 mm; SE = 5.14) occurring at the outlier pattern with 61.0‐m spacing. Results from this study indicate that reef spacing and horizontal extension are important factors to consider when designing an artificial reef program, especially those that target juvenile Red Snapper.Received February 2, 2012; accepted September 26, 2012

Effects of Eucheuma denticulatum Supplementation on Performance and Blood Chemistry of Juvenile Red Sea Bream Pagrus major

Effects of Eucheuma denticulatum Supplementation on Performance and Blood Chemistry of Juvenile Red Sea Bream Pagrus major

The Effects of Shielding on the Blackening of the Body Color of Juvenile and Adult Red Sea Bream (Pagrus major)

The Effects of Shielding on the Blackening of the Body Color of Juvenile and Adult Red Sea Bream (Pagrus major)

Supplementations of dl‐Methionine and Methionine Dipeptide in Diets are Effective for the Development and Growth of Larvae and Juvenile Red Sea Bream, Pagrus major

Growth trials for larvae and juvenile red sea bream, Pagrus major, were conducted to elucidate the efficacy of two molecular forms of methionine; dl‐methionine (dl‐Met) and methionine dipeptide (Met‐Met). For the larvae experiment, five experimental diets were formulated and fed to fish (42 mg) for 30 days. A diet which has 15% soy protein isolate served as the control diet. Similarly, test diets supplemented with dl‐Met and Met‐Met at 0.5%, which were either precoated by zein or intact, were also formulated. For the juvenile experiment, five experimental diets were formulated wherein the control diet contained 25% soy protein isolate. Test diets were supplemented with dl‐Met and Met‐Met at 0.75%, which were either coated by carboxymethycellulose or intact and fed to juveniles (0.75 g) for 56 days. The results of two feeding trials showed both dl‐Met and Met‐Met can be equally utilized by red sea bream larvae and juveniles. Coating the amino acid significantly improved both fish larval and juvenile growth performance. The development of digestive protease activity of larvae was significantly influenced by coating the amino acid, but the type of methionine was not a factor in changing the protease activity of larvae.

Discrimination of Juvenile Red Snapper Otolith Chemical Signatures from Gulf of Mexico Nursery Regions

AbstractAge‐0 red snapper Lutjanus campechanus from the 2005–2007 year‐classes were sampled in six regions across the Gulf of Mexico (Gulf) to develop nursery signatures from otolith element: Ca ratios (Ba:Ca, Mg:Ca, Mn:Ca, Sr:Ca, and Li:Ca) and stable isotope delta values (δ13C and δ18O). Element: Ca ratios were analyzed with sector field inductively coupled plasma mass spectrometry on dissolved right sagittae; isotope ratio mass spectrometry was employed to analyze pulverized left otoliths for δ13C and δ18O. Otolith chemical signatures were significantly different among regions in each year. Year‐class‐specific quadratic discriminant function analysis (QDFA) distinguished nursery regions with an accuracy of 82% for 2005, 70% for 2006, and 72% for 2007. However, samples were not obtained from all six study regions in 2005 and 2006. A QDFA of all year‐classes combined produced an overall classification accuracy of 70%, thus indicating that region‐specific otolith chemical signatures from adjacent sampling years could be used as surrogates for regions where samples were not obtained in a given year.Received February 7, 2012; accepted June 5, 2012