Juvenile Dog Research Articles

Isolated gall bladder torsion in a juvenile greyhound dog

Isolated gall bladder torsion in a juvenile greyhound dog

Trophic ecology and habitat use of an overexploited commercial snapper (Lutjanus jocu) in a tropical nursery estuary elucidated by stable isotopes

Despite the economic importance and susceptibility to overfishing of the dog snapper Lutjanus jocu, information on their first life stages and the use of nursery habitats remains scarce. Herein, we assessed the seasonal and ontogenetic changes on trophic ecology and habitat use of juveniles in a tropical estuary. Trophic position, prey assimilation, and trophic connectivity across dry and wet seasons and different size classes (< 100 and > 100 mm of total length – TL) were investigated using stable isotope analysis. A total of 57 individuals of 101.8 ± 51.9 mm (mean ± SD) of TL, ranging from 29 mm to 245 mm, had their trophic ecology and habitat use analyzed using dorsal muscle’s carbon (δ13C) and nitrogen (δ15N) stable isotope ratios. Significant differences were observed for δ13C according to the season (ANOVA, p < 0.001), but this pattern was not observed for δ15N (ANOVA, p = 0.471). In contrast, significant differences were observed for δ13C (ANOVA, p = 0.045) and δ15N (ANOVA, p < 0.001) according to the size classes. Trophic position increased according to body size (from 3.01 ± 0.09 to 3.57 ± 0.08 in smaller and larger individuals, respectively), although it remained similar between wet and dry seasons. Mangrove crab (Goniopsis cruentata) was the most assimilated prey across seasons, with a shift towards shrimps for the larger dog snappers. Our Bayesian mixing models revealed that juvenile dog snappers depend predominantly on trophic sources from sheltered estuarine habitats. However, smaller individuals have an isotopic composition more similar to marine sources, therefore, suggesting their recent ingression into the estuarine nursery habitat. In conclusion, our findings may serve as a basis for conservation plans, reinforcing the critical importance of preserving habitat connectivity during the early stages of fish species that use estuaries as nursery grounds and face threats from overexploitation.

Case report: Spontaneous mandibular body regeneration following unilateral subtotal mandibulectomy in a 3-month-old French bulldog.

To document a case of spontaneous regeneration of the mandibular body following subtotal mandibulectomy in a juvenile dog. A 3-month-old male intact French bulldog was presented with papillary oral squamous cell carcinoma located at the dorsal aspect of the molar region of the left mandible. Initial biopsy of the mass was performed by the primary care veterinarian. Complete clinical staging revealed no signs of metastasis. Computed tomographic images of the head showed minimal contrast enhancement of the mass with no signs of periosteal or bone involvement. Subtotal mandibulectomy was performed. Histopathology indicated complete excision of the tumor. The patient returned 8-weeks later for follow up and cleft palate surgical repair, at which time bone was noted in the mandibulectomy area on palpation. Repeat computed tomography of the head revealed complete regeneration of the left mandibular body from the level of the ramus to the mandibular symphysis. No treatment for malocclusion was necessary due to the reformation of a functional mandible. The present case demonstrates that spontaneous regeneration of the mandibular body is possible following subtotal mandibulectomy in immature dogs. Subtotal mandibulectomy is a radical procedure that can lead to long term complications including mandibular drift, malocclusion, and oral pain. This case report provides evidence that these sequelae may be mitigated or eliminated in young patients undergoing this procedure.

Juvenile primary acquired hypothyroidism in a dog with suspected renal dysplasia

AbstractA 1‐year 3‐month‐old, female, neutered crossbreed dog presented for investigation of polyuria, polydipsia and azotaemia. Subsequent biochemistry, endocrine testing and abdominal ultrasound identified hypercholesterolaemia, thyroglobulin autoantibody‐positive hypothyroidism, and IRIS stage II non‐hypertensive, non‐proteinuric suspected renal dysplasia, with a concurrent Escherichia coli urinary tract infection. The patient responded well clinically to levothyroxine treatment, with improvement in hypercholesterolaemia, azotaemia and demeanour at home. At follow‐up biochemistry assessment, hyperphosphataemia and hypercalcaemia (total and ionised) were documented, presumed to be driven by renal insufficiency and renal secondary hyperparathyroidism, although this aetiology was not definitively proven. Normalisation of these values was noted following renal diet implementation and addition of phosphate binders. This case highlights acquired primary hypothyroidism with evidence of thyroiditis in a juvenile dog with suspected congenital renal dysplasia.

Executive Functions as Measured by the Dog Executive Function Scale (DEFS) over the Lifespan of Dogs.

Executive Functions (EFs) are needed for effortful self-regulation of behaviour and are known to change over the lifespan in humans. In domestic dogs, EFs can be assessed through behavioural rating scales, such as the Dog Executive Function Scale (DEFS). The primary aim of this study was to investigate whether the DEFS, developed initially using a sample of adult dogs, can be used in juvenile (<1 year) and senior (>8 years) dogs. Confirmatory factor analysis of a juvenile and senior dog sample led to good model fit indices, indicating that juvenile and senior dogs' EF structure follows the same functional organisation as found in the DEFS. The secondary aim was to analyse the lifespan development of EFs. Analysis of subscale scores revealed multifaceted relationships with age for four subscales. Working Memory and Attention Towards Owner showed the u-shaped curve traditionally associated with the lifespan development of EFs. Forms of inhibition showed complex associations with age, i.e., Delay Inhibition declined in aging and Motor Regulation increased during aging. Training history and Working Status influenced performance independent of age. More highly trained dogs and working dogs exhibited higher EF skills. Training history appeared more important for EF in non-working dogs than working dogs, perhaps because all working dogs receive a high level of training.

Urogenital fistula associated with complex dystocia and emergency ovariohysterectomy in a juvenile dog

AbstractA 1‐year‐old, female, spayed dog was referred for urinary incontinence, first noted after a complex dystocia and caesarean section with ovariohysterectomy. Transabdominal ultrasonography and computed tomography with intravenous contrast administration revealed a fluid‐filled tubular structure caudodorsal to the urinary bladder. Routine cystourethroscopy was unremarkable. A retrograde cystourethrogram revealed a 15 mm long connection between the urethra and a structure presumed to be a uterine body remnant. During exploratory laparotomy, the uterine remnant and urethra were separated, leaving oval defects approximately 15 mm long in both; these were then closed. A urinary catheter was left in place for 6 days. A urethrogram performed during catheter removal confirmed closure of the fistula, and the dog was subsequently continent. To the authors’ knowledge, this is the first description of a urogenital fistula suspected to be associated with dystocia and emergency ovariohysterectomy in a dog.

Localized intestinal cryptococcosis and co‐parasitism in a juvenile dog

AbstractAn 8.5‐month‐old, male, entire golden retriever presented for 6‐month history of large bowel diarrhoea and vomiting. Giardiasis was previously treated. Clinical signs recurred despite empirical antibiotics, food trials, probiotics and psyllium. Physical exam revealed circumferential cobblestone‐like rectal mucosa. Rectal scrape cytology was consistent with cryptococcosis. Latex cryptococcal agglutination test was consistently negative. Thoracic radiographs were normal. Notable abdominal ultrasound findings included jejunal and suspected mesenteric cystic lymphadenopathy and multifocal areas of submucosal thickening. Abdominal lymph node aspirates revealed lymphoid hyperplasia. Faecal sedimentation and floatation revealed few Alaria species eggs and many Coccidia oocysts, respectively. Patient was treated with fluconazole, sulfadimethoxine, Drontal Plus and subsequently developed pancytopenia secondary to sulphonamide therapy requiring hospitalization, and recovered with supportive care and discontinuation of drug. Patient's clinical signs, rectal palpation and sonographic features resolved with continued fluconazole therapy. This case demonstrates localized intestinal cryptococcosis based on cytology with negative latex cryptococcal agglutination test and pancytopenia secondary to sulphonamide therapy for co‐parasitism.

Successful Management of Congenital Radial Head Subluxation in a Juvenile Dog Using Ultra-High Molecular Weight Polyethylene Suture Toggle

AbstractCongenital humeroradial subluxation was diagnosed by clinical examination and radiography in a 16-week-old female dog that presented for left forelimb lameness. This was treated surgically by open reduction followed by placement of an ultra-high molecular weight polyethylene suture from the craniolateral aspect of the proximal radius to the caudomedial aspect of the ulna, secured with a toggle and suture button. Postoperative findings were consistent with clinical improvement and radiographs were compatible with resolution of elbow subluxation. To our knowledge, this is the first case report of congenital humeroradial subluxation to be managed with an ultra-high molecular weight polyethylene suture toggle.

Detection of zoonotic vector-borne pathogens in domestic dogs in Giza, Egypt.

The public health implications of zoonotic vector-borne pathogens are numerous because domestic animals, such as dogs, live in close proximity to humans. Blood was collected from 116 domestic dogs in Cairo, Egypt from three different settings at the human-animal interface. The three settings the dogs came from were: privately owned animals seeking care at the Cairo University Faculty of Veterinary Medicine Clinic, non-laboratory reared research dogs maintained at the Cairo University Faculty of Veterinary Medicine, and an urban private animal rescue in Shabramont, Giza, Egypt. Enrolled animals were visually inspected for presence of flea or tick ectoparasites, Rhipicephalus sanguineus sensu lato ticks were recovered from 56 enrolled animals and a flea identified as Ctenocephalides felis was recovered from one animal. To test for past and/or current infection with vector-borne pathogens, conventional PCR and IDEXX SNAP® 4Dx® Plus were performed on whole blood. Pathogen targets included: Anaplasma spp., Ehrlichia spp., Babesia spp., Borrelia spp., Bartonella spp., Dirofilaria spp., and Rickettsia spp. Among dogs sampled across all locations, one dog was positive for Babesia sp. infection and one dog was positive for Anaplasma sp. infection as detected by PCR and confirmed by Sanger sequencing. Three additional dogs were positive for infection but had incomplete sequences obtained: two for Ehrlichia sp. and one for Borrelia sp. The SNAP® test results for all sampled dogs included: eight dogs positive for Anaplasma spp., 14 dogs positive for Ehrlichia spp., and five additional dogs positive for both Anaplasma spp. and Ehrlichia spp. SNAP® test results by sampling location showed that 66% of the dogs at the animal rescue were positive for Anaplasma spp. and/or Ehrlichia spp., 17% of the privately owned dogs at the Faculty of Veterinary medicine were positive for Anaplasma spp. and/or Ehrlichia spp., and none of the research dogs were positive for any of the targets on the SNAP® test. This high proportion of seropositivity in the animals sampled indicates a vector population which is not well controlled and a need for continued owner education and promotion of consistent use of preventive medications and the risk for zoonotic transmission.

Holistic management of renal nephroblastoma in a juvenile dog with severe cachexia: A case report

This report presents a case of a 2-month-old female German Shepherd dog with renal nephroblastoma and strong cachexia. The tumour was surgically removed and graded as a stage I with favourable histopathology, but due to its large size, adjuvant chemotherapy, including doxorubicin and vincristine, was recommended. Before, during and after chemotherapy, the body weight was gradually rebuilt with an appropriately balanced diet containing proper amounts of protein, fat and numerous supplements necessary for growth and development. Eighteen months after diagnosis, the dog was in good condition and had no metastases. In conclusion, this case study shows that close cooperation between oncologists and nutritionists is crucial for successful oncological therapy, especially in a cachectic patient.

Polyculture of Juvenile Dog Conch Laevistrombus canarium Reveals High Potentiality in Integrated Multitrophic Aquaculture (IMTA).

Simple SummaryThe dog conch (Laevistrombus canarium) is a marine gastropod mollusk widely distributed in the Indo-Pacific region. It is an economically crucial species; however, its population has been declining due to overfishing and overexploitation. Hence, we developed a novel polyculture and water-flow method for mass production of this species. Furthermore, the findings from this work also uncover the potentiality of L. canarium in integrated multitrophic aquaculture (IMTA) and its implication for aquaculture and resource restoration.Laevistrombus canarium, also known as dog conch, is a marine gastropod mollusk widely distributed in the Indo-Pacific region. It is an economically crucial species; however, its population has been declining due to overfishing and overexploitation. In this study, the suitable salinity for juvenile L. canarium was between 20 and 35‰. Diatoms and biological detritus by using flow-water from the fish pool were the most favorable diets for newly metamorphosed and 10 mm juveniles. In the polyculture experiment, L. canarium was cultured with whiteleg shrimp, tilapia, small abalone, purple sea urchin, and collector urchin. Better growth was found in all co-culture groups except with whiteleg shrimp. We also found that the polyculture system with or without substrates significantly affected the growth of juveniles. Additionally, we observed that water temperature was the most crucial factor for growth and survival; a water temperature of less than 10 °C might cause the death of L. canarium. We have proposed a novel polyculture and water-flow method for mass production of L. canarium and evaluated the feasibility and benefits of polyculture with other species. The findings from this work reveal the potentiality of L. canarium in integrated multitrophic aquaculture (IMTA) and its implication for aquaculture and resource restoration.

A Pilot Study of the Effects of Human Intervention on Canine Group Movement Behavior

Dogs are the oldest domesticated animals. The process of domestication of dogs is still unclear; however, they have established themselves as human partners and are sometimes more cooperative with humans than their conspecifics. In this study, to determine the effect of affiliative human presence on group behavior in dogs, we conducted short-time trials analyzing dog group movements. There was a hierarchical relationship in which juvenile dogs were aware of adult dogs, and adult dogs were aware of human movements. We also found that the age of the juvenile dog and the characteristics of their mothers may affect the movement behavior of juvenile dogs.

Concurrent and Predictive Criterion Validity of a Puppy Behaviour Questionnaire for Predicting Training Outcome in Juvenile Guide Dogs

Simple SummaryThe ability to predict later success in guide dog training can be of great benefit to assistance dog providers, such as those providing guide dogs, to ensure maximum resource and production efficiency and to maintain high welfare standards. This study evaluated the predictive capabilities of a behaviour questionnaire (the refined puppy walker questionnaire, r-PWQ) completed by volunteer carers of puppies (puppy walkers) for dogs aged eight months of age. The r-PWQ includes traits such as “Distractibility” and “Excitability”, which are common withdrawal reasons for many guide dogs. The predictive validity of the r-PWQ was compared to a well-known behaviour questionnaire, (Canine Behavioral Assessment and Research Questionnaire—C-BARQ). The results show that the r-PWQ can be used to predict guide dog outcome and may be better suited to guide dog populations than the C-BARQ.Working dog organisations regularly assess the behaviour of puppies to monitor progression. Here, we tested the predictive validity (for predicting success in guide dog training) of a shortened version of a previously developed juvenile dog behaviour questionnaire (the refined puppy walker questionnaire, r-PWQ) and compared it with the Canine Behavioral Assessment and Research Questionnaire (C-BARQ). The r-PWQ is used by Guide Dogs UK, whereas the C-BARQ was designed for pet dogs and is used by some other guide dog schools internationally. A cohort of dogs aged eight months (n = 359) were scored concurrently on the r-PWQ and C-BARQ. Analogous traits between the questionnaires were evaluated for internal consistency and association with training outcome and compared for concurrent validity. The r-PWQ was associated with training outcome for five scales (r-Excitability, Trainability, Animal Chase, r-Attachment and attention seeking and Distractibility) and the C-BARQ for two scales (Excitability and Separation-related behaviour). There were significant correlations between analogous C-BARQ and r-PWQ trait scores (p < 0.001) except for Separation-related behaviour and questionnaire scales had similar internal consistencies. The r-PWQ may be more suitable to use with guide dog schools. However, due to the correlation between analogous scales (except for “Distractibility”) some scales could be substituted for one another when reviewing the behaviour of dogs between guide dog schools using different questionnaires.

Medical management of a penile fracture with presumed pyelonephritis in a juvenile dog

A three-month-old male entire Bouvier des Flandres was presented for acute onset dysuria, haematuria, lethargy and severe pain on palpation of the penis. Further investigation revealed a parcellar fracture of...

Clinical, CT and ultrasonographic features of renal secondary hyperparathyroidism in a juvenile dog

A nine-month-old female entire Rottweiler was presented for further investigation of progressive, symmetric facial swelling over the maxilla of four weeks duration. Biochemistry revealed severe azotaemia with hyperphosphataemia. CT revealed...

Arthroscopically Guided Proximal Tibial Epiphysiodesis Screw Placement as Treatment of a Bilateral Partial Cranial Cruciate Ligament Injury in a Juvenile Dog

AbstractThis study aimed to describe surgical technique of arthroscopically guided proximal tibial epiphysiodesis screw placement in the treatment of a bilateral partial cranial cruciate ligament (CrCL) rupture in a 5.6-month-old Golden Retriever. A 19-kg, 5.6-month-old female Golden Retriever was diagnosed with bilateral partial CrCL rupture. Proximal tibial epiphysiodesis was performed bilaterally under arthroscopic guidance. Arthroscopic stifle joint inspection was performed bilaterally with a 2.7 mm arthroscope. A medial port at the level of the distal one-third of the patella was used as camera portal. A lateral instrument port was positioned at the level of the distal one-third of the patella. A 3.0 mm diameter headless cannulated self-compressive screw was placed into the centre of the tibial cranial intercondyloid area as parallel as possible to the tibial shaft axis under arthroscopic control. Screw head was buried. Arthroscopic guidance allowed good accuracy in screw placement, specifically in its insertion point. However, the screw orientation was less satisfactory. The tibial plateau angle progressively decreased in both stifles to achieve 15 degrees on the left and 16 degrees on the right at the last follow-up. A varus deformity developed on both stifles. No clinical consequences were observed. Recurrence of right pelvic lameness occurred 1 year postoperatively because of a medial meniscal lesion, which necessitated partial caudal meniscectomy. Arthroscopically guided proximal tibial epiphysiodesis is technically feasible and is a minimally invasive treatment of CrCL injury in a juvenile dog.

Quantifying the Biomagnification Capability of Arctic Wolf and Domestic Dog by Equilibrium Sampling.

The mechanism underlying contaminant biomagnification is a decrease in the volume (V) and the fugacity capacity (Z) of food during digestion in the gastrointestinal tract. Traditionally, biomagnification is quantified by measuring contaminant concentrations in animal tissues. Here, we present a proof-of-concept study to noninvasively derive the thermodynamic limit to an organism's biomagnification capability (biomagnification limit -BMFlim) by determining the ratio of the V·Z-products of undigested and digested food. We quantify Z-values by equilibrating food and feces samples, which have been homogenized and spiked with polychlorinated biphenyls (PCBs), with silicone films of variable thickness coated on the inside of glass vials. We demonstrate the feasibility of this method for wolf (Canis lupus hudsonicus) and domestic dog (Canis lupus familiaris). For an adult wolf eating a relatively lean meat diet, a BMFlim (averaged over several PCB congeners) of approximately 41 was observed, whereas the BMFlim reached 81 for an adult domestic dog eating a lipid-rich diet. Besides the dietary lipid content that strongly affects the Z-value of the diet, the capability of an animal to digest its diet also influences the BMFlim by controlling the Z-values of their feces and the volume reduction of the food in the gastrointestinal tract. Less efficient digestion leads to a lower BMFlim in a juvenile dog (approximately 35) compared to its older self, even though their diets had similar lipid contents. The effect of the volume reduction (VD/VF ranging from 4 to 15) was comparable to the effect of the Z-value reduction (ZD/ZF from 3 to 20).

Specific treatment and outcome of urethrorectal fistula associated with type 1 atresia ani in a juvenile male dog

A four-month-old, entire male, German wirehaired pointer presented with tenesmus due to type I atresia ani and with urination observed through this stenosed anal opening. A positive contrast retrograde urethrogram...

Frame-shift variant in the CHRNE gene in a juvenile dog with suspected myasthenia gravis-like disease.

Frame-shift variant in the CHRNE gene in a juvenile dog with suspected myasthenia gravis-like disease.

Colonic vascular ectasia (angiodysplasia) in a juvenile dog

An eight-month-old female English springer spaniel was presented with weight loss and severe haematochezia. Upper and lower endoscopy identified small intestinal inflammatory bowel disease and a vascular malformation within the descending colon. The colonic lesion was excised at coeliotomy and identified histopathologically as a colonic vascular ectasia. All clinical signs resolved following surgery and continued dietary management. To the authors' knowledge this is only the second published report of CVE in a juvenile dog and the first to survive to long term follow up.