Gendarussa Research Articles

GC–MS and In-silico studies of Neer Nochi (Justicia gendarussa Burm.f.) against Estrogen Receptor Alpha (ERα)

GC–MS and In-silico studies of Neer Nochi (Justicia gendarussa Burm.f.) against Estrogen Receptor Alpha (ERα)

Medicinal Plants Used for Postpartum Recuperation in an Upland Community in Leon, Iloilo, the Philippines

The utilization of medicinal plants has been a longstanding tradition – providing natural remedies with cultural and therapeutic significance, especially in the context of postpartum care. Thus, this study aims to document the usage of medicinal plants in postpartum recuperation, specifically in herbal steaming and bathing in the upland community of Leon, Iloilo, the Philippines. Semi- structured interviews were conducted with 34 key informants using a snowball sampling method from October–November 2022 and in March 2024, thereby resulting in the documentation of 70 medicinal plant species distributed in 64 genera and 35 families. The Fabaceae family was most represented with seven species, followed by Poaceae with six species, and Lamiaceae with five species. Most of the documented species are non-native (51%) and cosmopolitan, categorized as “least concern” (50%) in the IUCN Red List of Endangered Species. A variety of medicinal plants were documented – including trees (57%), herbs (20%), shrubs (17%), and climbers (6%). Roots (64%) and leaves (24%) were the plant parts used most often, and decoction was the primary form of preparation. These medicinal plants can be found growing within the community's vicinity, with most of them (51%) found in forested areas and the rest (49%) being cultivated. The use value (UV) and family use value (FUV) indices were calculated to evaluate the plant’s importance. Pittosporum pentandrum (Blanco) Merr. had the highest (0.91) UV, followed by Citrus maxima (Burm.) Merr. and Justicia gendarussa Burm.f. (0.88), and Bambusa spinosa Roxb. (0.85). Pittosporaceae (0.91) and Hypericaceae (0.62) had the highest FUVs. The findings of this study will provide a foundation for additional scientific validation of the biological properties of the documented plants, specifically focusing on the most valued species, and raise awareness for the preservation of cultural practices and beliefs regarding postpartum care for women.

Effect of Papaya Seeds (Carica papaya) and Gandarusa Leaves (Justicia gendarussa) on the Spermatozoa Quality of Male Mice

Papaya seeds (<em>Carica papaya </em>L<em>.</em>) and gandarusa leaves (<em>Justicia gendarussa </em>Burm.f.) are considered medicinal plants that can be used as natural male contraceptives. Papaya seeds contain alkaloids, triterpenoids, saponins, and papain. Meanwhile, the gandarusa leaves contain flavonoids. These compounds have the function of antifertility for men. The purpose of this study was to determine the effect of papaya seeds (<em>Carica papaya </em>L<em>.</em>) ethanol extracts, gandarusa leaves (<em>Justicia gendarussa </em>Burm.f<em>.</em>), and its combination on motility, viability, and morphology of male mice spermatozoa (<em>Mus musculus</em><em>)</em> most effective decrease concentration on motility, viability, and morphology of male mice spermatozoa (<em>Mus musculus</em>). This research was an experimental study. The samples of this study were 18 male mice with a body weight of 20-30 grams, aged 3-5 months, and were divided into six groups. The treatments given were control, papaya seed ethanol extract, gandarusa leaves ethanol extract, and its combinations to concentrations of P1 Na CMC1%, P2 (papaya seed 100mg/kgW), P3 (gandarusa leaf 50mg/kgW), P4 (papaya seed : gandarusa leaf 50:50 mg/kgW), P5 (papaya seed : gandarusa leaf 50:100 mg/ kgW), P6 (papaya seed : gandarusa leaf 100:50 mg/kgW). The extract was given for 35 days. On the 36<sup>th</sup> day, the mice were dissected to take their epididymis to observe the spermatozoa's quality (motility, viability, morphology). Those observations used a light microscope. Data were analyzed using one-way ANOVA and LSD post hoc tests. This study resulted differences in P1, P2, P3, P4, P5, and P6 towards motility, viability, and morphology of spermatozoa (<em>p</em>< 0.05). The conclusion of this study showed that both single and combination ethanol extracts of papaya seeds and gandarusa leaves could reduce the motility, viability, and morphology of spermatozoa of male mice, and the effective dosage in reducing motility, viability, and morphology of spermatozoa is P3 gandarusa leaves 50mg/kgW.

Analgesic effects of semi-solid extract from four medicinal herbs (Clinacanthus nutans (Burm. f.) Lindau, Vitex negundo L., Justicia gendarussa L., Piper lolot L.) in mice

Objectives: This study was conducted with the aim of evaluating the experimental analgesic effects of the semi-solid extract from four folk medicinal herbs: Clinacanthus nutans (Burm. f.) Lindau, Vitex negundoL., Justicia gendarussa L., and Piper lolot L. (BNTL extract). This study provides a scientific basis for further research on the effects of a topically applied natural-origin remedy. Materials and methods: Swiss albino mice, 20±2 g in weight, provided by the Pasteur Institute in Ho Chi Minh city. Hot-plate method and mechanical pain method were used to determine the analgesic effects of BNTL extract. Results: BNTL extract applied topically showed only a statistically significant effect on prolonging the response time compared with the control group to mechanical agents. BNTL extract helps prolong the time to appear temperature response in white mice, but has no statistical significance compared to the control group in the hot plate model. Conclusions: BNTL extract did not show central analgesic effects, but did show analgesic effects in experimental mechanical pain models.

In silico approaches of gandarusa (Justicia gendarussa Burm. f.) potential for osteoporosis prevention via EGF pathway

Context: On a cellular level, osteoporosis is a metabolic bone disease caused by osteoclastic bone resorption that is not offset by osteoblastic bone synthesis. This increases the risk of fractures by making bones weak and brittle. The selective inactivation of the epidermal growth factor receptor (EGFR) in osteoblast lineage cells demonstrated that EGFR promotes bone formation by increasing the number of mesenchymal progenitors. Active compounds of gandarusa (Justicia gendarussa Burm. f.) are potentially playing crucial roles in EGFR activation. Aims: To evaluate the potential of J. gendarussa for osteoporosis prevention via EGF pathway in silico. Methods: A GC-MS approach was used to screen the active compounds from 70 and 95% methanol extraction, which was continued with molecular docking analysis by PyRx v0.8. The visualization and amino acid interactions were also revealed using Discovery Studio software R17. ADME analysis was performed using the SwissADME webserver to consider whether the best compound could be classified as a drug following Lipinsky rule’s of five. Results: The top five compounds with high binding affinity were selected from a total of 33 active compounds detected. One compound, namely eslicarbazepine, has a -6.7 kcal/mol affinity score and passes the threshold for drugs bioavailability, supported by the data of MW compounds 254.28 kDa, low rotatable bonds 1 bond, hydrogen bond acceptors and donors (2), high GI absorption, and ability to permeant to blood-brain barrier system. Conclusions: This finding added new insight into J. gendarussa extract potential as anti-osteoporosis in humans. Nevertheless, in vitro and in vivo experiments are necessary to confirm the potential.

Studies on Anticancer Effect of Methanolic Leaf Extract of Justicia gendarussa on Lung Cancer Cell Line

ABSTRACT Objective: The aim of study’s goal was to look into the anticancer efficacy of a methanolic extract of Justicia gendarussa against a lung cancer cell line. Materials and Methods: Cell viability assays and cell and nuclear morphology examinations were used to evaluate the anticancer efficacy against methanolic extract of Justicia gendarussa on lung cancer cell lines. The IC50 doses were calculated using different concentrations of Justicia gendarussa extract (0, 10, 20, 40, 60, and 80 μg/mL). Results: The results of MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) assay revealed that the percentage of viability in treated cells was significantly lower as compared with untreated control groups, which represented as 100%, and an inhibitory concentration of 40 μg/mL was observed. Under a phase-contrast microscope, morphological changes revealed cell shrinkage and cytoplasmic membrane blebbing. The apoptotic nuclei (intensely colored, broken nuclei, and compacted chromatin) were examined under a fluorescence microscope. Conclusions: The outcome of the research work on Justicia gendarussa was investigated for anticancer properties. The results revealed the proapoptotic and cytotoxic effects of Justicia gendarussa extract on lung cancer cell lines. From the above results and findings, it could be concluded that the Justicia gendarussa methanolic leaf extract exhibited potent anticancer activity against a lung cancer cell line. Further study needs to be conducted to investigate the active chemicals in the extract as well as the molecular mechanisms underlying its anticancer benefits.

UHPLC-Q-Orbitrap-HRMS-based untargeted metabolomics reveal metabolites change in Justicia gendarussa and its antioxidant capacity at different doses of nitrogen fertilizer.

This study delves into the antioxidant potential of Justicia gendarussa, commonly known as gendarussa, and its response to varying doses of nitrogen fertilizer. Gendarussa exhibits the potential for antioxidant activity. The diverse ecological conditions in which it thrives may influence its biological activity and lead to inconsistent production of secondary metabolites. Nitrogen, a pivotal factor in secondary metabolite production in plants, has become a focal point of this research. This research aims to determine the optimal nitrogen fertilizer dose on gendarussa antioxidant capacity and metabolites using a metabolomics approach. Employing a randomized block design for cultivation, the investigation revealed that a maximum harvest weight of 10.9 g/aerial parts of the plant was achieved with 270 kg/ha of nitrogen fertilizer. This study explored the DPPH, ABTS, FRAP, and CUPRAC assays of antioxidant effect, and found insignificant differences between the various nitrogen treatments. UHPLCQ-Orbitrap HRMS was employed to identify 30 metabolites in positive and 18 in negative ionization modes. Gendarusin A, a major metabolite in gendarussa, is identified in both positive and negative ionization. PCA and heatmap analysis successfully categorized these metabolites in the aerial parts of gendarussa at different nitrogen fertilizer dosages. Based on the metabolomics approach, variations in nitrogen fertilizer made metabolites at doses of 90 kg/ha had higher relative concentrations of metabolites compared to doses of 180 kg/ha and 270 kg/ha. So, 90 kg/ha are the optimal nitrogen fertilizer dose for cultivation and utilization strategies.

Performance of dye-sensitized solar cells with mixed three natural pigments and reduced graphene oxide as a counter electrode

This work overviews the effect of combining three natural dyes as a photosensitizer for dye-sensitized solar cells (DSSC). Reduced graphene oxide in this work was used as a Pt-free counter electrode. The dyes consist of curcuminoid (Curcuma longa), chlorophyll (Justicia gendarussa), and anthocyanins (Clitoria ternatea). The effect of absorbance spectrum, Light Harvesting Efficiency (LHE), bandgap energy, and performance of DSSC were also studied. From the result, combination dyes have a broader absorbance spectrum and LHE than a single dyes solution, while the redox potential of the dye produces a similar gap energy (HOMO/LUMO) due to the solvent used to extract the dye, which is around 1.76–1.79 eV. The best result was achieved by a combination of three dyes, Yellow-Green-Blue, which has a short-circuit current density (Jsc) of 6.50 mA/cm2, an open-circuit voltage (Voc) of 0.94 V, a fill factor of 31.2 %, and efficiency of 1.91 %. The performance of the DSSC is not only due to a large amount of photon energy absorbed and adsorbed on the TiO2 surface and the fast electron diffusion rate due to the large LHE and narrow gap energy. Therefore, combining natural dye and using rGO as the counter electrode can improve the performance of DSSC optimally.

Antioxidant, Antiinflammatory, and Anti Uric Acid Bioactivity Correlation of some Family Acanthaceae Plants (A Review)

Uric acid is a naturally occurring antioxidant that is produced in the body as a byproduct of the enzymatic breakdown of xanthine. Inflammation of the blood vessel cells in the human body can be brought on by abnormally high amounts of uric acid in the serum. An acute inflammatory response often manifests with symptoms similar to those of gout attacks. XO is a major contributor to the production of superoxide free radicals, which are a factor in chronic inflammatory diseases. Existing drugs have been used to treat gout and inflammatory diseases, but have negative effects in humans. Medicinal plants are a source of active chemicals, which are particularly significant in the process of developing new medications due to the variety and distinctive chemical structures of these active compounds. Justicia gendarussa, Andrographis paniculata (Burn.f.) Nees, Ruellia tuberosa L, and Clinacanthus nutans Lindau are examples of plant species in the Acanthaceae family. The results of existing publications show that the bioactive compounds contained in these plants have a relationship between antioxidant activity, anti-uric acid and anti-inflammatory properties.

Effects of Shading and Nitrogen Fertilizer on Growth and Physiology of Gandarusa (Justicia gendarussa Burm. F.)

Gandarusa (Justicia gendarussa Burm. F.) is a shrub used in herbal medicine, but knowledge of optimal cultivation methods for enhancing plant growth and metabolite yield is limited. This research aimed to evaluate the effect of shading and nitrogen fertilizer on the growth, photosynthetic parameters, and total sugar content of gandarusa. A split-plot experimental design was used with shading (S) (0% (S0), 25% (S25), and 50% (S50)) as the main plots and nitrogen fertilizer (N) (0 (N0), 90 (N90), 180 (N180), and 270 (N270) kg ha-1) as the subplots. The results showed that the combination of S0 and N270 was the most effective treatment for plant growth, indicated by the highest values of plant height and the number of leaves and branches. It also yielded high sugar content, with a value range of 72-76 mg g-1 leaves wet weight. The combination of S0 and N0 produced the highest photosynthetic rate (Pn) in the plant at 23.91 mol CO2 m-2 s-1, and total chlorophyll content was highest with S25 and N270. Based on the results, shading decreased Pn, sugar production, and growth of gandarusa, while nitrogen fertilizer enhanced them. However, there was no interaction between shading and fertilizer on sugar production and growth of gandarusa, except for Pn.

Guided Analysis of Selected Ethnobotanical Acanthaceous Plants for Anti-Inflammatory Properties

Acanthaceous plants have emerged as important therapeutic plants on a global scale, producing biologically active chemical compounds with larvicidal, antimicrobial, and cytotoxic properties. In traditional Thai medicine, the crude extracts of these plants, including Clinacanthus nutans, Justicia gendarussa, and Rhinacanthus nasutus, have been used to detoxify bug bites and some skin illnesses caused by fungus and viruses. This study argued for investigating acanthaceous plant extracts for alternate advantages such as anti-inflammation. The presence of anti-inflammatory components in extracts was examined using HPLC in order to evaluate their potential. Lupeol, a prospective anti-inflammatory triterpene found in medicinal plants, was employed as a chemical marker. The anti-inflammatory activities of extracts containing the active chemical were studied utilizing protein denaturation and inhibition of nitric oxide production in RAW 264.7 cells stimulated with LPS. Also, the extract of combined active plants was assessed. Lupeol was detected in the tested extracts in quantities ranging from 0.25 to 1.22% w/w. It was found in the highest concentration in the extract of Clinacanthus nutans and in the lowest concentration in the extract of Justicia gendarussa. All extracts of plants containing the active ingredient exhibited anti-inflammatory effects. Their IC50 values ranged between 0.87 and 29.28 mg/mL. The extract made from a combination of plants that contained lupeol demonstrated the most potent anti-inflammatory effect, with a 0.6-fold difference when compared to diclofenac. This research implied that investigating acanthaceous plant extracts containing this active agent might be more beneficial in the design of herbal recipes with anti-inflammatory characteristics.

A Panoramic Review of Traditional Use, Phytochemical Composition and Pharmacology of Justicia Gendarussa Burm f

Justicia gendarussa Burm f. also known as Willow-leaved justicia (family Acanthaceae) is a plant species native to china. This shrub has been used in Indian and Chinese traditional medicine. This plant can be located in different Asian countries. Various phytoconstituents are identified from this plant like Flavonoids, alkaloids, steroids, triterpenoids, phenolic compounds, saponin and glycosides. The main biologically active constituents found are Lupeol, stigmasterol, Aromadendrin, naringenin, Vitexin, apigenin, Justiprocumin A and B, Patentiflorin A and Justidrusamide A-D. These constituents help to contributes various pharmacological activities like anti-inflammatory, anti-oxidant, anti-viral, anti-helmintic, anti-bacterial, anti-fungal, anti-angiogenic, hepatoprotective action and antifertility activity. The current review focus on the botanical description, traditional uses, phytochemical constituents and pharmacological activities of the plant Justicia gendarussa Burm f.

Overview of the Justicia Genus: Insights into Its Chemical Diversity and Biological Potential.

The genus Justicia has more than 600 species distributed in both hemispheres, in the tropics and temperate regions, and it is used in the treatment of numerous pathologies. This study presents a review of the biological activities of plant extracts and isolated chemical constituents of Justicia (ACANTHACEAE), identified in the period from May 2011 to August 2022. We analyzed over 176 articles with various biological activities and chemical compound descriptions present in the 29 species of Justicia. These have a variety of applications, such as antioxidant and antimicrobial, with alkaloids and flavonoids (e.g., naringenin) the most frequently identified secondary metabolites. The most observed species were Justicia gendarussa Burm., Justicia procumbens L., Justicia adhatoda L., Justicia spicigera Schltdl, and Justicia pectoralis Jacq. The frontier molecular orbitals carried out using density functional theory (M062X and basis set 6-311++G(d,p) indicate reactive sites for naringenin compound and a chemical reaction on phytomedicine activity. The energy gap (206.99 kcal/mol) and dimer solid state packing point to chemical stability. Due to the wide variety of pharmacological uses of these species, this review points toward the development of new phytomedicines.

Screening of Preliminary Phytochemicals, Molecular Identification, and Antimicrobial and Anti‐Inflammatory Activity of Justicia gendarussa

Justicia gendarussa is a well‐known medicinal plant from ancient times that has been using to treat a variety of ailments. The main objectives of the current study were to assess the phytochemicals, molecular identification of the plant, and determination of MIC, as well as antimicrobial and anti‐inflammatory activities of various types of plant extracts (aqueous, ethanolic, methanolic, diethyl‐ether, dichloromethane, and ethyl‐acetate extracts) of J. gendarussa (leaves) against 11 human pathogens, including bacteria and fungi. In the present study, J. gendarussa was identified as a medicinal plant based on ITS gene amplification by PCR. The screening of the presence of important phytochemicals that carry high significance from a therapeutic standpoint was also carried out. The antimicrobial activity was assessed by following the disc diffusion and broth dilution procedures. The highest antimicrobial activity was observed against S. aureus (16 mm), S. flexneri (15 mm), and C. albicans (15 mm) compared with gentamicin (10 μg/disc) and fluconazole (25 μg/disc). Besides this, methanolic extract showed the highest anti‐inflammatory activity (76% membrane protection). The MIC values (8–2048 μg/ml) of aqueous extract were observed against both types of bacteria and fungi, with the highest MIC value recorded at (2048 μg/ml) for S. flexneri. This study is the primary investigation in Bangladesh where molecular identification of this medicinal plant was carried out and the anti‐inflammatory activity of this plant was evaluated by the HRBC membrane stabilization method. In conclusion, our findings suggest that this plant may be useful in research and development for discovering a broad spectrum of modern herbal medicines.

Response surface modeling to optimize sonication extraction with the maceration method for the phenolic content and antioxidant activity of Justicia gendarussa Burm f.

Response surface modeling to optimize sonication extraction with the maceration method for the phenolic content and antioxidant activity of Justicia gendarussa Burm f.

Comparative Study of Leaf Anatomy and Micromorphology of Selected Justicia Species from Peninsular Malaysia

Justicia has been recognized as one of the largest genera in the Acanthaceae family with 600 species have been identified. Most researchers have problems identifying Justicia species especially if the samples are incomplete. Other than that, there is no comprehensive study on Justicia species, especially in Peninsular Malaysia. Therefore, a comprehensive study of leaf anatomy and micromorphology of three selected Justicia species which are Justicia adathoda, Justicia gendarussa and Justicia procumbens were conducted in order to assess the leaf anatomical and micromorphological variations that would be useful to identify the plant species. The methods used in this study involved several methods such as cross-section using a sliding microtome and observation under the scanning electron microscope (SEM) for the epidermis of abaxial and adaxial surfaces. Collenchyma, sclerenchyma and mucilage cells can be found either in the petiole or the midrib in all species studied. Amphistomatic stomata have also been recorded in all species examined. Meanwhile, several differences were discovered in this study, including the type of trichomes, the pattern of vascular bundles, the type of epicuticular wax structures, cuticle ornamentation and the presence of cystolith cells. In conclusion, the characteristics of some Justicia species in Peninsular Malaysia can be identified using leaf anatomy and micromorphology.

Justicia gendarussa (BURM F.) (WILLOW-LEAVED Justicia) FROM PENINSULAR MALAYSIA: LEAF ANATOMY AND MICROMORPHOLOGY

Acanthaceae or the Acanthus family has been commonly used by local peoples as traditional medicines, especially in PeninsularMalaysia. Justicia gendarussa, also known as ‘daun rusa’ or ‘genda rusa,’ treats inflammatory disorders, asthma, hepatic injuries,arthritis, and digestion problems. Many taxonomists nowadays struggle to identify Acanthaceae species due to morphologicalsimilarities and incomplete parts during the identification process. Other than that, there has been no comprehensive study ofJusticia gendarussa leaf anatomy to date. Therefore, this study aims to describe the leaf’s anatomical and micromorphologicalcharacteristics that would be useful to identify this plant species. The leaf anatomical study involved several methods such ascross-section using a sliding microtome and observation under a light microscope. While the leaf micromorphology methodinvolved the observation under a scanning electron microscope (SEM) of the epidermis of abaxial and adaxial surfaces. Theresult of this study showed the presence of collenchyma, sclerenchyma, and mucilage cells in the petiole and midrib. The patternsof petiole and midrib vascular bundles were described as the main vascular bundle (opened system with continuous rings of thevascular bundle) with two additional vascular bundles located the above right and left of the main vascular bundle near eachwing. The cystolith cells are present in the epidermis and parenchyma cortex while glandular and non-glandular trichomeswere recorded in the species studied. In conclusion, comprehensive data for the leaf anatomical and micromorphologicalcharacteristics can be used to supplement data in the identification of Justicia gendarussa.

Phenolic and flavonoid content with agro morphological characters of 12 accessions of Justica gendarussa grew in Indonesia

Abstract. Marliani N, Artika IM, Rafi M, Syukur M, Galingging RY, Nurcholis W. 2022. Phenolic and flavonoid content with agro morphological characters of 12 accessions of Justica gendarussa grew in Indonesia. Biodiversitas 23: 5101-5107. Justicia gendarussa Burm. f is a medicinal plant used in traditional and modern medicine. This study was conducted to determine the agro morphological characters, phenolic and flavonoid content of 12 accessions of J. gendarussa plants obtained from various locations in Indonesia and then grown in the same conditions and places. The agro morphological characteristics include plant height, number of leaves, number of branches, wet weight, and dry weight of leaves and stems. Total phenolic content (TPC) and total flavonoid content (TFC) were analyzed by spectrophotometric method. The results showed significant differences in the characters of 12 accessions of J. gendarussa (p<0.05). Accessions of BK4 (Bekasi 4) and KP (Kejayan Pasuruan) had the best agro morphological characteristics. Accessions of BK4 (Bekasi 4) and BG (Bogor) had the best growth rates. The highest TPC value was obtained from TG (Trenggalek) accession (0.752 mg GAE/g DW) and the highest TFC (1.899 mg QE/g DW from LK (Langkat) accession. The best accessions based on agro morphological characters were BK4 (Bekasi 4) and KP (Kejayan Pasuruan), and based on the content of polyphenols were TG (Trenggalek) and LK (Langkat). The accessions of BK4, BG, TG, and LK could be further developed into superior varieties of J. gendarussa.

First Report of Cassytha filiformis parasitizing Justicia gendarussa in Malaysia

First Report of Cassytha filiformis parasitizing Justicia gendarussa in Malaysia

Optimization Extraction for Total Phenolic, Flavonoid Contents, and Antioxidant Activity with Different Solvents and UPLC-MS/MS Metabolite Profiling of Justicia gendarussa Burm.f

Abstract Justicia gendarussa Burm.f contains polyphenol, which has pharmacological activity. Therefore, this study aims to show the potential of antioxidant compounds in J. gendarussa. The extraction method used was an experimental design that maximized phenolic recovery with the highest antioxidant activity from the leaves of this plant. Furthermore, a simplex-centroid design was used to evaluate the various extractor solvents (water, ethanol, and hexane) and their binary or ternary mixtures, while UPLC-MS/MS analysis was used to identify bioactive compounds. The total phenol (TPC) and flavonoid content (TFC) of the optimized extracts were determined using Follin-Ciocalteu and colorimetric methods, as well as their antioxidant activity were determined using the 2,2-diphenyl-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. The results was optimized to a value of 0.844 using water-hexane solvents with a predicted TPC, TFC, DPPH, and FRAP of 112.076 mg GAE/g, 34.926 mg QE/g, 45.477 µmol TE/g, and 353.611 µmol TE/g, respectively. Additionally, the UPLC-MS/MS analysis showed 62 compounds such as phenolics, isoflavonoids, alkaloids, terpenoids, alcohols, and other groups of components. This study shows the potential of antioxidant compounds J. gendarussa. Keywords: Extraction optimization, Justicia gendarussa Burm.f, Simplex centroid design, Polyphenols, Antioxidant